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	Acid-fast Stain
		The ability of the bacteria to resist decolorization with ACID alcohol confers acid fastness to the bacterium.
		Acid-fast bacteria, of which there are very few---the major genus Mycobacterium, have a high concentration of mycolic acid, a lipid, in their walls.
		Add the decolorizer acid alcohol (1% HCl + ethanol) and decolorize for 15-20 seconds.
	www.rlc.dcccd.edu /mathsci/reynolds/micro/lab_manual/AF_stain.html (310 words)

	Reference.com/Encyclopedia/Acid-fast
		Acid-fastness is a physical property of some bacteria referring to their resistance to decolorization by acids during staining procedures.
		The high mycolic acid content of certain bacterial cell walls, like those of Mycobacterium, is responsible for the staining pattern of poor absorption followed by high retention.
		The most common staining technique used to identify acid-fast bacteria is the Ziehl-Neelsen stain, in which the bacteria are stained bright red and stand out clearly against a blue background.
	www.reference.com /browse/wiki/Acid-fast (248 words)

	Acid-fast stain
		The slide is then washed with an acid solution, and a counter-stain is applied.
		The bacteria that retain the first dye are acid-fast because they resist the acid wash. Bacteria that wash free of the first dye and take the counterstain are nonacid-fast.
		Preparation for the test depends on the sample to be collected.
	www.healthscout.com /ency/1/003766.html (280 words)

	Epochem dyestuffs: Acid dyes.
		Fast Light Yellow 2G / Brilliant Yellow 2G
		Violet N-FBL / FB / BL / Fast Milling Violet FBL
		Fast Blue P-L / BW Acid Blue 82
	www.epochem.com /products/dyes.htm (428 words)

	Use of Fluorochrome Staining for Detecting Acid-Fast Mycobacteria
		You should be aware that the concentrations of both the acid and the alcohol in the decolorizing solution used for fluorochrome staining are less than the concentrations in the decolorizing solution used for fuchsin-staining; thus, you cannot use the same decolorizing solution for both methods.
		One acid fast bacillus in the center of the field is obvious.
		If only 1-2 acid fast organisms are seen after observing more than 70 fields at 400x or 450x magnification, the smear should be reported as "equivocal or doubtful" and should be repeated.
	www.phppo.cdc.gov /dls/lta/afb_fluoromx_trans.aspx (4237 words)

	TB Acid Fast
		The lower magnification used with fluorescent staining allows us to observe a greater area of the smear, and the fluorescing organisms are easier to observe than the pale pink staining mycobacteria using one of the carbolfuchsin stains.
		Since mycobacteria grow slowly, the acid fast smear plays an important role in the early diagnosis of mycobacterial infections.
		Acid fastness is not specific for Mycobacterium tuberculosis, as all species of mycobacteria possess this characteristic (i.e.
	sld.state.nm.us /tb/acid.htm (245 words)

	Medmicro Chapter 33
		Mycobacteria are slender, curved rods that are acid fast and resistant to acids, alkalis, and dehydration.
		The mycolic acids containing extremely long (C60 to C90) side chains are joined to the muramic acid moiety of the peptidoglycan by phosphodiester bridges and to arabinogalactan by esterified glycolipid linkages.
		The resistance to acids and alkalis is useful in the isolation of mycobacteria from contaminated clinical specimens such as sputum.
	gsbs.utmb.edu /microbook/ch033.htm (6623 words)

	Ellis and Zabrowarny method for Acid Fast Bacilli
		The property of acid fastness is related to the carbon chain length of the mycolic acid found in any particular species (Lyon H 1991).
		The mycolic acid (and other cell wall lipids) present a barrier to dye entry as well as elution (washing out with solvent) and this is partly overcome by adding a lipophilic agent to a concentrated aqueous solution of basic fuchsin and partly by heating.
		Acetic acid is a strong irritant and a corrosive.
	home.primus.com.au /royellis/afb.htm (783 words)

	Eating Food Too Fast Speeds Acid Reflux
		May 23, 2003 - Fast eating, not just fast food, can increase the risk of acid reflux after meals, according to a new study.
		GERD occurs when stomach acids wash up into the esophagus and cause symptoms such as chest pain and heartburn.
		Acid reflux episodes were reported a total of 12.5 times after the five-minute meal versus 8.5 times after the 30-minute meal.
	www.webmd.com /heartburn-gerd/news/20030523/eating-food-too-fast-speeds-heartburn (293 words)

	GramStain
		Acid fast organisms resist decolorization with acid alcohol.
		After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast.
		Non acid fast organisms and tissue cells stain blue.
	www.spcollege.edu /hec/vt/VTDE/ATE2639LGS/gramstain.htm (501 words)

	Acid-fast stain; Baltimore, Maryland MD
		The slide is then washed with an acid solution, and a counter-stain is applied.
		The bacteria that retain the first dye are acid-fast because they resist the acid wash. Bacteria that wash free of the first dye and take the counterstain are nonacid-fast.
		Preparation for the test depends on the sample to be collected.
	www.goodsam-md.org /1911.cfm (354 words)

	Part ii : acid-fast staining procedures
		Always add acid slowly to alcohol, not vice versa.
		In countries where the acquisition of alcohol may be problematic, a solution of 25% sulphuric acid may be used as decolourising agent.
		Always add acid slowly to water, not vice versa.
	www.afrihealth.net /tb/microacid.htm (793 words)

	Sensitivity of Acid-Fast Staining for Mycobacterium tuberculosis in Formalin-fixed Tissue -- Fukunaga et al. 166 (7): ...
		Cocito C. Biological, chemical, immunological and staining properties of bacteria isolated from tissues of leprosy patients.
		Popper HH, Winter E, Hofler G. DNA of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissue in tuberculosis and sarcoidosis detected by polymerase chain reaction.
		Ninet B. Detection of mycobacterial nucleic acids by polymerase chain reaction in fixed tissue specimens of patients with human immunodeficiency virus infection: Swiss HIV Cohort Study.
	ajrccm.atsjournals.org /cgi/content/full/166/7/994 (2616 words)

	Prokaryotic Cell Structure: The Acid-Fast Cell Wall
		The arabinogalactan/mycolic acid layer is overlaid with a layer of polypeptides and mycolic acids consisting of free lipids, glycolipids, and peptidoglycolipids.
		The mycolic acids and other glycolipids also impede the entry of chemicals causing the organisms to grow slowly and be more resistant to chemical agents and lysosomal components of phagocytes than most bacteria.
		The mycolic acid molecules and the peptidoglycan fragments bind to specific toll-like receptors and NODs on the surface of macrophages causing them to release cytokines such as tumor necrosis factor-alpha (TNF-alpha).
	student.ccbcmd.edu /courses/bio141/lecguide/unit1/prostruct/afcw.html (580 words)

	Histology Lab: STAIN FOR ACID FAST BACILLI
		These are [fl]-hydroxy carboxylic acids with chain lengths of up to 90 carbon atoms.
		Ethanol is rapidly oxidised, in the body, to carbon dioxide and water and does not produce permanent damage to the central nervous system.
		ACETIC ACID: is a corrosive substance and will cause severe damage to exposed skin.
	www.nottingham.ac.uk /pathology/protocols/afb.html (746 words)

	acid fast - OneLook Dictionary Search
		We found 15 dictionaries with English definitions that include the word acid fast:
		Tip: Click on the first link on a line below to go directly to a page where "acid fast" is defined.
		Phrases that include acid fast: acid fast stain, acid fast bacilli stain, acid fast organism
	www.onelook.com /?w=acid+fast&ls=a (134 words)

	Acid-Fast (Mycobacteria) Broth-Based Culture and Smear and Susceptibility
		Requests with only a written order and no test number indicated will be processed according to Default Test Order for Ambiguous Orders.
		First morning sputum (not saliva) (three separate specimens from three separate days are recommended), fasting gastric aspirate, induced sputum, whole blood, tissue, biopsy, bronchial aspirate, urine, skin, cerebrospinal fluid, bone marrow, body fluid, stool.
		Swabs of exudate from skin sources are acceptable, otherwise, swab specimens should not be submitted.
	www.labcorp.com /datasets/labcorp/html/chapter/mono/mb025400.htm (2227 words)

	Emerging Disease Issues - Histopathology and Acid Fast Staining
		Emerging Disease Issues - Histopathology and Acid Fast Staining
		Browsers that can not handle javascript will not be able to access some features of this site.
		Natural resources; hunting; prohibition for feeding deer and elk; extend sunset.
	www.michigan.gov /emergingdiseases/0,1607,7-186-25804_25810-74336--,00.html (393 words)

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		Decosup provides toll blending and private labeling packaging.
		Non-Hydrochloric acid formulation provides a safer product and non haz-mat shipping.
	www.decosup.com /services.html (226 words)

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