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Topic: Actomyosin

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	Reviewers about "Evidence of a new type of protein-protein interaction"
		It is like that under centrifugal force the natural actomyosin ended up at the bottom of the tube and the desensitized actomyosin, being less dense was on top.
		Actomyosin is insoluble in solutions with low ionic strength (I used these solutions).
		Actomyosin can be sol only in 0,5 M KCl solution, but I did not use such solutions.
	www.actomyosin.spb.ru /reviewers.htm (1699 words)

	Skeletal muscle force and actomyosin ATPase activity reduced by nitric oxide donor -- Perkins et al. 83 (4): 1326 -- ...
		Isometric actomyosin ATPase activity was measured at 15°C by using a fluorescence-coupled enzyme assay (14).
		actomyosin ATPase is proportional to slope of NADH fluorescence,
		in the pCa 9 actomyosin ATPase was not observed.
	jap.physiology.org /cgi/content/full/83/4/1326 (5267 words)

	phleomycin-muscle.html
		A correlation of 0.99 was obtained in DNA and actomyosin synthesis for right and left muscles in these cases, suggesting that the reaction would be all or none within the range of traumatization used in these investigations.
		Actomyosin synthesis was judged by the incorporation into this protein complex of L-lysine-14C (uniformly labeled 223 mc/mmole) given intraperitoneally in two doses of 10.0 ul each at 94 and 117 h after wounding.
		Concentration of actomyosin was determined from its ultraviolet absorption maximum (275 mu), after the Folin reaction.
	www.indiana.edu /~pietsch/phleomycin-muscle.html (3869 words)

	Myofibril - Wikipedia, the free encyclopedia
		Thick filaments consist primarily of the protein myosin, held in place by titin filaments.
		The protein complex composed of actin and myosin is sometimes referred to as "actomyosin."
		In striated muscle, such as skeletal and cardiac muscle, the actin and myosin filaments each have a specific and constant length on the order of a few micrometers, far less than the length of the elongated muscle cell (a few millimeters in the case of human skeletal muscle cells).
	en.wikipedia.org /wiki/Actomyosin (657 words)

	actomyosin - Search Results - MSN Encarta
		Myosin, the protein chiefly responsible for muscle contraction, combines with actin, another muscle protein, forming actomyosin, the different...
		actomyosin - definition of actomyosin in the Medical dictionary - by...
		NMR (2000) 32: 167-178 Evidence of a New Type of Protein-Protein Interaction: Desensitized Actomyosin Blocks Ca 2+ -Sensitivity of the Natural One.
	encarta.msn.com /actomyosin.html (151 words)

	NEL, Cardiac Mechanoenergetics in silico
		In the third section, the studies of connection between mechanical stress developed by actomyosin complex and ATPase activity of this complex at tissue [Vendelin et al., 2000,Engelbrecht et al., 2000] and left ventricle [Vendelin et al., submitted] levels are overviewed.
		This transformation occurs in the actomyosin complex, aligned into the filaments in the heart, as a result of cyclic interaction of myosin heads with actin filaments [Huxley, 1990,Irving, 1985,Saeki, 1995].
		This hierarchical structure incorporates the cross-bridge state distribution functions and the variables describing the activation of the actomyosin complex by calcium ions as used in [Vendelin et al., 2000].
	www.nel.edu /23_1/NEL230102R01_Vendelin.htm (5589 words)

	Quantum Biology - Myosin Coherence (Site not responding. Last check: 2007-10-23)
		While on the microscale the axon clearly functions as a kind of wire that propagates an electric current to the synapse, on the nanoscale it serves the purpose of transporting vesicles containing the neurotransmitters required for signal transduction across the synapse.
		The vesicles (nanoscale vessels or containers) are transported by the actomyosin molecular motors described on the previous page.
		Actin and myosin are the two components of actomyosin, which is a ubiquitous complex used for muscle contraction in addition to its vesicle transport function in neurons.
	www.nanoword.net /library/weekly/000625b.php (405 words)

	Wenise's Research (Site not responding. Last check: 2007-10-23)
		The actomyosin system (Figure 1) has been studied extensively as a model of chemomechanical transduction, with a major focus on the conformational changes arising within actin and myosin that lead to force generation.
		My work focuses on: (1) Determining residues in actin that are important in the actomyosin interaction; and, (2) Testing the hypothesis that specific charged residues in the N-terminus of actin are important in the transition from weakly- to strongly-bound actomyosin complexes.
		Since the transition from weakly- to strongly-bound actomyosin states directly precedes the powerstroke, free energy resulting from this transition (A-M-ADP-Pi to A-M*-ADP-Pi) is considered to be instrumental in the transduction of the chemical energy of ATP to mechanical force.
	www.chem.ucla.edu /~wong/work.html (415 words)

	Elizabeth Vallen Research Projects
		The utilization of an actomyosin contractile ring for cytokinesis is conserved evolutionarily; however, many questions about the mechanism and regulation of contraction remain unanswered.
		Because the actomyosin system is redundent in S. cerevisiae, null mutations that affect ring contraction can be isolated and monitored.
		We are exploiting yeast lacking hof1 to identify genes and proteins that are important for the function of the actomyosin pathway.
	www.swarthmore.edu /NatSci/evallen1/projects.html (1136 words)

	Actomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo -- Nishii and Ogihara ...
		Actomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo -- Nishii and Ogihara 126 (10): 2117 -- Development
		Actomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo
		We conclude that the principal role of actomyosin in
	dev.biologists.org /cgi/content/abstract/126/10/2117 (295 words)

	Definition of actomyosin - Merriam-Webster Online Dictionary
		Learn more about "actomyosin" and related topics at Britannica.com
		Find more about "actomyosin" instantly with Live Search
		See a map of "actomyosin" in the Visual Thesaurus
	www.webster.com /dictionary/actomyosin (52 words)

	Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor Biophysical Journal - Find Articles
		Hasselbach (1956) reported an excellent correlation between the rate of NTP hydrolysis by actomyosin and the isometric tension of glycerinated muscle fibers.
		After the success of fluorescence microscopy in visualizing fluorescently labeled single actin filaments (Yanagida et al., 1984), a more versatile and stable assay was developed wherein actin filaments move over surfaces coated with myosin filaments (Kron and Spudich, 1986), or proteolytic fragments of myosin (Toyoshima et al., 1987).
		First, it has been shown that the velocity of actin filaments sliding over myosin-coated surfaces and the velocity of myosin-coated beads along actin cables is analogous (with slight differences, depending on conditions) to the speed of unloaded shortening of muscle fibers (Sheetz et al., 1984; Homsher et al., 1992).
	www.findarticles.com /p/articles/mi_qa3938/is_200101/ai_n8933078 (925 words)

	Modest actomyosin energy conservation increases myocardial postischemic function -- Blunt et al. 288 (3): H1088 -- AJP ...
		Modest actomyosin energy conservation increases myocardial postischemic function -- Blunt et al.
		with the hypothesis that moderate decreases in actomyosin ATPase
		Cumulative absolute (A) and relative (B) Ca -activated actomyosin ATPase activity as a function of Ca concentration in ventricular myofibrils from PTU-treated and control rats.
	ajpheart.physiology.org /cgi/content/full/288/3/H1088 (4802 words)

	Goldman (Site not responding. Last check: 2007-10-23)
		These values are compared to the corresponding rate constants obtained with myosin and actomyosin in solution to determine whether or not the packing of the proteins into the normal filament lattice influences the reaction rates and to determine which elementary reactions are modulated by mechanical stress and strain on the proteins.
		A biochemical scheme for the actomyosin ATPase in solution is given in the General Introduction to this Special Topic section and is hereafter referred to as Scheme 1.
		Reasons to expect important differences between the kinetics of the actomyosin ATPase in solution and in fibers are discussed in the General Introduction.
	www.uphs.upenn.edu /pmi/Abstracts/yegabs5.html (226 words)

	Vladimir Matveev. Protoreaction of Protoplasm
		For instance, the maximal stabilizing effect of ethanol on ribonuclease is achieved at 2000 mM ethanol (3), whereas 680 mM is sufficient in the case of actomyosin (16).
		In this connection, it is interesting to compare data obtained on living muscle and glycerol-treated muscle models (see 28 for references), and on isolated actomyosin (12, 16, 17).
		Matveev, V.V., Evidence of a new type of protein-protein interaction: desensitized actomyosin blocks Ca -sensitivity of the natural one.
	www.actomyosin.spb.ru /protoreaction.htm (7253 words)

	78B-8 Application of antifreeze proteins in food preservation: efficiency of antifreeze proteins in actomyosin ... (Site not responding. Last check: 2007-10-23)
		Unfortunately, the gel-forming quality of actomyosin is demolished during frozen storage.
		The mechanisms are believed to involve freeze-induced denaturation of actomyosin and ice recrystallization.
		The actomyosin deterioration process was induced by a slow freezing process and three cycles of freeze-thaw processes.
	ift.confex.com /ift/2000/techprogram/paper_4614.htm (269 words)

	Myosin light chains: These were discovered by analytical ultracentrifugation; succinylated, or K2CO3 treated myosin ...
		As actin is added to myosin in increasing amount, part of the myosin combines with actin, forming the high molecular weight actomyosin (AM) and 2 peaks are seen.
		The viscosity of actomyosin is much higher than the sum of its individual components actin and myosin.
		In suspension, at physiological ionic strength, the myosin-actin binding is measured under conditions when both myosin and actomyosin are insoluble, that is they sediment at low centrifugal fields.
	www.uic.edu /classes/phyb/phyb516/myosinfunctionu.htm (1585 words)

	Cortical Actomyosin Breakage Triggers Shape Oscillations in Cells and Cell Fragments Biophysical Journal - Find Articles
		The proposed mechanism for breakage of the actomyosin cortex may be used for cell polarization.
		As MTs have been shown to regulate actomyosin contractility, possibly due to the Rho-kinase pathway, the spontaneous behavior of the actomyosin cortex in the absence of MTs is thus an important issue in cell dynamics.
		Since such oscillations are observed only in situations where adhesion is weak, they may reveal a specific regime of actomyosin dynamics that would be unveiled in the absence of adhesion.
	www.findarticles.com /p/articles/mi_qa3938/is_200507/ai_n14879419 (1032 words)

	Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds -- Mandato ...
		By 21 min the zone is almost gone near the original break point, but by 30 min, a new zone has assembled (arrowheads).
		Wound-induced assembly and closure of an actomyosin purse string in Xenopus oocytes.
		Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast.
	www.jcb.org /cgi/content/full/154/4/785 (7283 words)

	Interactive Fly, Drosophila (Site not responding. Last check: 2007-10-23)
		During cellularization, the actomyosin cytoskeleton forms a hexagonal array and drives invagination of the plasma membrane between the nuclei located at the cortex of the syncytial blastoderm.
		It is a driver of leading edge cell contractility from the earliest stages of dorsal closure, but it is also required for restraining the leading edge epithelial cells and maintaining a coherent and taut epithelial margin during the later phases of dorsal closure, particularly as the epithelial faces are being zippered together.
		The dual role for the actomyosin cable during dorsal closure, operating both as a 'purse string' and as a cell restrainer, is likely to be a finely balanced operation.
	www.sdbonline.org /fly/cytoskel/zipper4.htm (20408 words)

	Research and Reviews: Meat 2001, Special Circular 183-02
		The physicochemical state of the myofibrillar proteins, myosin and actomyosin, confer functionality to meat systems and thereby have a direct role in determining the quality and value of processed meat (Yasui et al., 1982; Camou and Sebranek, 1991).
		Thus, myosin and actomyosin are the most important factors for the development of the binding properties of thermally induced meat protein gels (Samejima et al., 1969; Samejima, et al., 1981 and 1982).
		Not only do myosin and actomyosin contribute to the rigidity (firmness) of thermally induced meat gels, they are also critical to the water holding capacity (WHC) and high cooking yields of comminuted meat products such as sausages (Hamann, 1988; Smith, 1988).
	ohioline.osu.edu /sc183/sc183_5a.html (679 words)

	Effects of {kappa}-opioid receptor activation on myocardium -- Pyle et al. 281 (2): 669 -- AJP - Heart and Circulatory ...
		The reduction in actomyosin Mg -ATPase activity was associated with an increase in whole heart
		The effects of PKC on actomyosin Mg -ATPase activity depends on which myofilament protein is phosphorylated
		Protein kinase C phosphorylation of cardiac troponin I and troponin T inhibits Ca -stimulated MgATPase activity in reconstituted actomyosin and isolated myofibrils, and decreases actin-myosin interactions.
	ajpheart.physiology.org /cgi/content/full/281/2/H669 (5125 words)

	Phosphorylation of myosin light chains in mouse fast-twitch muscle associated with reduced actomyosin turnover rate -- ...
		Phosphorylation of the 18,000-dalton light chains of the fast-twitch myosin in mouse extensor digitorum longus muscles was correlated with reduction in the rate of the actomyosin adenosinetriphosphatase in vivo, but neither of these changes occurred in the soleus muscle.
		These results suggest that actomyosin interactions can be down-regulated by a reversible covalent modification of myosin light chains, that a mechanism for thick-filament regulation occurs in vertebrate skeletal muscle, and that the expression of this regulation may be limited to a specific fiber type.
		Actomyosin energy turnover declines while force remains constant during isometric muscle contraction.
	www.sciencemag.org /cgi/content/abstract/217/4562/835 (327 words)

	Interactive Fly, Drosophila (Site not responding. Last check: 2007-10-23)
		While actomyosin regulation and microtubule bundling may be the primary regulatory roles of these factors, there are additional ways that the Pbl RhoGEF and RacGAP50C could influence both the actin and microtubule-based cytoskeleton.
		Although the molecular mechanism behind this reorganization of the actomyosin cytoskeleton is not known, it indicates that the motor protein is able to mediate associations between the actomyosin and microtubule cytoskeletons.
		The formation and maintenance of both the central spindle and the actomyosin ring could be mediated by physical interactions between interzonal microtubules and components of the contractile ring.
	www.sdbonline.org /fly/cytoskel/pavart4.htm (14090 words)

	Crystal Protein Store: Actomyosin
		The structure of actomyosin, produced as a 3D etching inside a a block of clear glass 150 x 75 x 75mm (6" long).
		Part of the price goes to Dr. Bagshaw for use of the design, to be used towards future molecule models.
		The white LED stand fits the actomyosin crystal in horizontal orientation.
	www.crystalprotein.com /store/actomyosin.html (217 words)

	Rigor mortis - Wikipedia, the free encyclopedia
		The biochemical cause of rigor mortis is hydrolysis of ATP in the muscle tissue, the chemical energy source required for movement.
		Myosin molecules devoid of ATP become permanently adherent to actin filaments to form actomyosin complex, causing muscles to become rigid.
		As a result, the actomyosin complex is broken down and muscles become "soft" again.
	en.wikipedia.org /wiki/Rigor_mortis (433 words)

	Drc1p/Cps1p, a 1,3-{beta}-Glucan Synthase Subunit, Is Essential for Division Septum Assembly in Schizosaccharomyces ...
		of the actomyosin ring during mitosis and cytokinesis.
		Actomyosin rings are unusually stable in drc1-191 mutant cells:
		Actomyosin rings are assembled at the onset of mitosis and disassembled
	www.genetics.org /cgi/content/full/153/3/1193 (5885 words)

	Changes in actomyosin ATP consumption rate in rat diaphragm muscle fibers during postnatal development -- Sieck et al. ...
		Changes in actomyosin ATP consumption rate in rat diaphragm muscle fibers during postnatal development -- Sieck et al.
		In reaction 1, ATP is hydrolyzed by the actomyosin ATPase to ADP and P
		Cross-bridge cycling kinetics, actomyosin ATPase activity and myosin heavy chain isoforms in skeletal and smooth respiratory muscles.
	jap.physiology.org /cgi/content/full/94/5/1896 (3439 words)

	Hsp90 Protein in Fission Yeast Swo1p and UCS Protein Rng3p Facilitate Myosin II Assembly and Function -- Mishra et al. ...
		of the actomyosin ring is required for and precedes septum assembly.
		Swo1p function is essential for assembly of a normal actomyosin ring.
		Importance of a myosin II-containing progenitor for actomyosin ring assembly in fission yeast.
	ec.asm.org /cgi/content/full/4/3/567 (5335 words)

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