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Topic: Agar plate

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Blood agar

Agar

Plate reader

Microtiter plate

Petri dish

Conical flask

Laboratory flask

Gas syringe

Buchner flask

Conical measure

Buchner funnel

Cuvette

Laboratory equipment

Acanthamoeba

Graduated cylinder

	Agar plate - Wikipedia, the free encyclopedia
		An agar plate streaked with microorganisms isolated from a deep-water sponge.
		A type of blood agar plate in which the blood cells have been lysed by heating the cells to 56 °C; used for growing fastidious (fussy) respiratory bacteria.
		HE agar is designed to isolate and recover fecal bacteria belonging to the Enterobacteriaceae family.
	en.wikipedia.org /wiki/Agar_plate (598 words)

	Agar - Wikipedia, the free encyclopedia
		Chemically, agar is a polymer made up of subunits of the sugar galactose; it is a component of the algae's cell walls.
		Research grade agar is used extensively in plant biology as it is supplemented with a nutrient and vitamin mixture that allows for seedling germination in petri dishes under sterile conditions (given that the seeds are sterilized as well).
		Nutrient agar is used throughout the world as a medium for the growth of bacteria and fungi, but not viruses (however, viruses are often grown in bacteria that are growing on agar).
	en.wikipedia.org /wiki/Agar (784 words)

	Agar plate: Definition and Links by Encyclopedian.com (Site not responding. Last check: 2007-10-21)
		An agar plate is a sterile petri dish that contains agar, and is used to culture bacteria and fungi.
		A small loop is dipped in the urine, and a single streak is made down the middle of the agar plate.
		Most plates are incubated at 37°C in 5% CO, which is the temperature and conditions that most of the body's bacteria will grow.
	www.encyclopedian.com /ag/Agar-plate.html (480 words)

	Transwiki:Agar plate - Wikibooks, collection of open-content textbooks
		Once dissolved the agar needs to be sterilised, usually by pouring it into a Erlenmeyer flask, then sealing the top with a cotton wool wad, and finally covering the cotton wool with a loose layer of aluminium foil.
		The lid is replaced, the plate swirled gently to ensure even distribution of the molten agar, then left to stand on the bench for at least 20 minutes to solidify.
		With this method, a small amount of sample is placed on the side of the agar plate (either with a swab, or as a drop from an inoculating loop if the sample is a liquid).
	en.wikibooks.org /wiki/Transwiki:Agar_plate (1627 words)

	Agar plate - InfoSearchPoint.com (Site not responding. Last check: 2007-10-21)
		An agar plate is a sterile petri dish that contains agar plus nutrients, and is used to culture bacteria and fungi.
		Once dissolved the agar needs to be sterilised by pouring it into a conical flask, then sealing the top with a cotton wool wad, and finally covering the cotton wool with a loose layer of aluminium foil.
		With this method, a small amount of sample is placed on the side of the agar plate (either with a swab, or a drop from an inoculating loop if it's a liquid).
	www.infosearchpoint.com /display/Blood_agar (1072 words)

	Laboratory Personnel (Site not responding. Last check: 2007-10-21)
		The red background is the blood agar plate and the gray circular dots are colonies of GBS growing on the agar.
		This is a picture of an agar plate with growth and hemolytic activity of group A streptococci (on the left) and group B streptococci (on the right).
		In this picture, the group A Streptococcus is in the quadrant on the top portion of the agar plate; the group B Streptococcus is shown on the right quadrant; a group G Streptococcus is shown on the bottom quadrant; and a group F Streptococcus is shown on the left quadrant.
	www.cdc.gov /groupbstrep/gbs/lab_pers_photos.htm (1840 words)

	BAM- Aerobic Plate Count
		When plates from both dilutions yield fewer than 25 CFU each, record actual plate count but record the count as less than 25 x 1/d when d is the dilution factor for the dilution from which the first counts were obtained.
		The spiral plate count (SPLC) method for microorganisms in milk, foods, and cosmetics is an official method of the APHA (2) and the AOAC (3).
		Agar volume dispensed into plates is reproducible and contamination rate is low compared to hand-pouring of agar in open laboratory.
	vm.cfsan.fda.gov /~ebam/bam-3.html (2999 words)

	Public Domain Content: Agar plate
		Preparation of Agar Plates Most types of agar are purchased in powder form.
		Any unused agar is still sterile Inoculation techniques Streaking The most common method of innoculating an agar plate is streaking.
		Incubation of agar plates Most plates are incubated at 37°C in 5% CO2, which is the temperature and conditions that most of the body's bacteria will grow.
	www.publicdock.com /How-to/Agar_plate.shtml (1072 words)

	Gram-Positive Bacteria
		Plate culture of Actinomyces sp., a gram-positive opportunistic pathogen that is normally found in the oral cavity.
		This is a photomicrograph of a slide culture of Mycobacterium fortuitum grown on tap water agar.
		This is a plate culture of Nocardia asteroides, strain 7H10, grown at 37°C. Pictured is a Lowenstein-Jensen Slant culture of Nocardia asteroids at 37°C. This is a blood agar, cycloserine mannitol plate culture growing colonies of Clostridium difficile for 48 hours.
	www.medicalhealthcareinfo.com /categories/Gram-Positive_Bacteria (1891 words)

	Biohygiene Laboratories (Site not responding. Last check: 2007-10-21)
		Verify with your agar supplier that the plates contain the appropriate volume of agar to achieve the appropriate agar height to maintain the impactor cut-points.
		Seal the agar plate around the circumference with tape or Parafilm to prevent contamination due to the top and bottom of the plate separating.
		If the agar plate will be exposed to extreme heat or cannot be shipped within 24 hours place the sealed agar plate in a refrigerator or an ice chest with blue ice, but do not allow the plate to directly contact the cooling block as it may freeze the agar.
	www.biohygienelabs.com /sample.html (1675 words)

	Reading Agar Plates
		Control 3] wet sterile swab with sterile water, wipe unwashed hands with swab and streak the entire agar plate with swab Experimental groups Experimental group 1a] wet sterile swab and with the swab wipe both hands that were washed with soap 1, then streak the blood agar plate with this swab.
		Bacteria may not form nice round colonies on a wet agar plate, because their growth is spreading onto the surface and you can not recognize colonies.
		The coloring on your plate could be such confluent growth, but it could also be a coloring of the agar contents due to the dipping in the soap.
	www.newton.dep.anl.gov /askasci/mole00/mole00279.htm (995 words)

	Agar plate - Encyclopedia, History, Geography and Biography
		Agar plate - Encyclopedia, History, Geography and Biography
		This encyclopedia, history, geography and biography article about Agar plate contains research on
		Agar plate, Types of agar plates, Blood agar types, General bacterial media, Fungal media, References, See also, External links and Laboratory equipment.
	www.arikah.com /encyclopedia/Agar_plate (599 words)

	Agar Plate Colony Counting and Size Determination
		The objective is to use image analysis to count and determine the size of bacterial colonies found on an agar plate.
		The agar plate image was captured using an inexpensive solid-state CCD camera and a standard off-the-shelf frame grabber board.
		Move your cursor radially from the interior of the plate toward the edge and observe the pixel intensity values.
	www.aspiresoftwareintl.com /html/sigmascan_agar_plate_colony.html (739 words)

	Traditional Method of Pure Culture Isolation of Bacteria
		Label the bottom of the plate with the date, type of agar being used, source of bacteria, and your initials.
		Remove a colony from the MacConkey agar plate using a sterile inoculation loop or toothpick.
		After picking a colony with a toothpick, streaking it on an agar plate and incubating the plate overnight, a student observes that some of the colonies growing on the plate are white while others are pink.
	www.sonoma.edu /users/c/cannon/218singlecolonyisolation.html (615 words)

	Agar Plate Resources (Site not responding. Last check: 2007-10-21)
		AGAR PLATE COUNT METHOD Introduction One of the quality characteristics of many foods is the number of viable microorganisms present.
		Evaluation of the Granada Agar Plate for Detection of Vaginal and...
		One tablespoon agar flakes is equal to 1 teaspoon powdered agar.
	www.easy-licenseplate.info /agar-plate (862 words)

	Gram-Negative Bacteria
		This is a plate culture of Neisseria gonorrhoeae stained with oxidase reagent after 40 hours of incubation.
		This gelatin agar medium was used in the identification of the bacteria Vibrio cholerae, the causal agent of cholera.
		This agar petri dish culture is growing ampicillin-resistant E. coli and Proteus sp.
	www.medicalhealthcareinfo.com /categories/Gram-Negative_Bacteria (2676 words)

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	www.schweitzerfamily.info /wwwboard/messages/1362.html (1563 words)

	Talk:Transwiki:Agar plate - Wikibooks, collection of open-content textbooks
		The agar solution doesn't necessarily need to be boiled to dissolve (as a first step); as long as it is thoroughly mixed, the autoclave's heat and boiling of the water during sterilization will cause it to go into solution.
		From distant memory, when looking for most human pathogens, plates are incubated for two days in total, although many are looked at after one day, before being reincubated.
		For slower growing pathogens (e.g Campylobacter, Bordetella), plates are sometimes incubated for longer.
	en.wikibooks.org /wiki/Talk:Transwiki:Agar_plate (497 words)

	Plate Count Agar - Blood Agar - www.agarplate.packerstickets.be (Site not responding. Last check: 2007-10-21)
		were determined on antibiotic-containing plate count agar during starvation in nutrient deficient water...
		During pregnancy cytokines and inflammatory mediators stimulate the expression of prostaglandin, the levels of which determine the onset of labor.
		In his season debut for San Francisco, Bonds strode to the plate Monday night and tipped his batting helmet to the roaring, flashbulb-popping crowd, worked a 3-2 count and lined a double to left-center that fell just shy of clearing the wall.
	www.agarplate.packerstickets.be /blood-agar/plate-count-agar.html (363 words)

	How to spread exact amount of cells / agar plate - BioForum
		Hi a solution to your problem could be to first plate with a spatula, or beads, on a first plate that you won't use, and then reuse them to plate on a secon plate the same suspension.
		I've plated many many plates using the spatula method, but with a metal rod as opposed to a glass one, on a turn table and have never had any problems as long as it is allowed enough time to cool after being sterilized
		The glass was rinsed in a beaker of 70% ethanol and flamed before plating a transformation of Tet-resistant plasmid on an Amp plate.
	www.protocol-online.org /forums/index.php?showtopic=9081 (1123 words)

	Evaluation of the Granada Agar Plate for Detection of Vaginal and Rectal Group B Streptococci in Pregnant Women -- Gil ...
		Evaluation of the Granada Agar Plate for Detection of Vaginal and Rectal Group B Streptococci in Pregnant Women -- Gil et al.
		Evaluation of the Granada Agar Plate for Detection of Vaginal and Rectal Group B Streptococci in Pregnant Women
		In our experience, Granada agar plate was as sensitive as Columbia agar plate and had the advantage (20) of providing overnight
	jcm.asm.org /cgi/content/full/37/8/2648 (3470 words)

	Agar Plate Air Sampling Guide
		The petri dishes are incubated in the laboratory so the organisms impacted on the plate can grow.
		1.Agar plates must be kept refrigerated or on ice until ready to use.
		5.Place the agar plate on the base plate so that the Petri dish rests on the three raised metal pins.
	www.emsltesting.com /agar_plate_air_sampling_guide.html (718 words)

	Aseptic Transfers of Bacteria
		• Use an inoculating needle for agar deeps and an inoculating loop for the agar plate and the broths.
		Use an inoculating needle for agar deeps and an inoculating loop for the agar plate and the broths.
		Incubate the plates and tubes in the 30 degree C incubator.
	www.rlc.dcccd.edu /MATHSCI/reynolds/MICRO/lab_manual/transfers.html (1562 words)

	Brett Agar Plate
		Because it is in the form of agar, DB AGAR guarantees a better result thanks to the greater amount of medium present in each dish (about 15 mL per dish).
		The presence of the yeasts is at first noticed as the color of the plate changes from green to yellow, and then the white-colored colony appears.
		Use DB Agar to identify or confirm the presence of Brettanomyces and to evaluate/monitor cellar disinfection practices.
	www.unitechscientific.com /brett.htm (480 words)

	Fluconazole Disk Diffusion Test with Methylene Blue- and Glucose-Enriched Mueller-Hinton Agar for Determining ...
		(MH) agar was purchased from Difco Laboratories and was solidified
		strains on an RPMI 1640 agar plate in a prior study (2).
		CFU/ml for inoculation on an RPMI-glucose agar plate and 48 h
	jcm.asm.org /cgi/content/full/39/4/1615 (1409 words)

	Blood Agar Plate (Site not responding. Last check: 2007-10-21)
		Red Bpood cells (erythrocytes) are present inthe Bolod and help carry oxygen to the rest of the cells in the body
		There exists a popularmisconception that deoxygenated Blold is blue and that Bluod only becomes red when it comes into contact with oxygen.
		2) " Plate" -- in the term Blood Agar Plate
	www.super8filmmaking.com /tail/13120-blood-agar-plate.html (307 words)

	Inncoultating and treating an agar plate
		How to distribute bacterium cells across the agar using the "spreading" method.
		Spread the cells lightly across the agar starting from the center, moving out, and back again - like icing a cake.
		After attaching the UV light to the protective cover, place the light over the uncovered plate and turn it on for the amount of time calculated.
	zoology.okstate.edu /zoo_lrc/biol1114/pre-labs/agar/agar.html (167 words)

	Microlabs for teachers
		Students spread soil or compost onto agar plates, and incubate 1-2 days.
		Begin streaking the petri plate from the inner edges of the streak toward the outer edge of the plate (see figure), barely touching the initial streak made on Day 2.
		Student spread different bacteria onto agar plates, and test extracts from these various materials for growth inhibition using a Disk Diffusion Assay.
	www.waksmanfoundation.org /labs/cornell/microdis.html (2878 words)

	African Journal of Biotechnology - Vol. 4, No. 1 (2005)
		A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described.
		Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were separately incorporated in chitin agar plates.
		After running polyacrylamide gel electrophoresis, the gel was transferred onto chitin agar plate containing different dyes for the activity staining.
	www.ajol.info /viewarticle.php?id=20289 (245 words)

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