
	Where results make sense

Topic: Agarose

Related Topics

Agarose gel electrophoresis

DNA ladder

Molecular biology

Polysaccharide

Agar

Agar plate

Max Perutz

Algae

Phosphorus

DNA

Francis Crick

Suffrage

Politics of El Salvador

Rosalind Franklin

Opera

In the News (Sat 19 Dec 09)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	Millipore - Technical Library - DNA Extraction from Agarose Gels with Montage Gel Extraction Kit or Ultrafree-DA ...
		Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and captures the resultant gel slurry in the sample filter cup.
		As the agarose is compressed at 5,000 x g, DNA is extruded from the gel's pores.
		Centrifugation forces the agarose through the gel nebulizer, converting it to a fine slurry that is captured by the sample filter cup.
	www.millipore.com /publications.nsf/docs/6dllx5 (537 words)

	Agarose (Site not responding. Last check: 2007-11-07)
		Agarose is a natural colloid extracted from sea weed.
		Agarose is a linear polysaccharide (average molecular mas about 12,000) made up of the basic repeat unit agarobiose, which comprises alternating units of galactose and 3,6-anhydrogalactose.
		Agarose is usually used at concentrations between 1% and 3%.
	www.bergen.org /AAST/Projects/Gel/agarose.htm (168 words)

	Agarose gel electrophoresis (Site not responding. Last check: 2007-11-07)
		Agarose gel electrophoresis (2) is employed to check the progression of a restriction enzyme digestion, to quickly determine the yield and purity of a DNA isolation or PCR reaction, and to size fractionate DNA molecules, which then could be eluted from the gel.
		Although 0.7% agarose gels typically are used, in cases where the accurate size fractionation of DNA molecules smaller than 1 kb is required, a 1, 1.5, or 2% agarose gel is prepared, depending on the expected size(s) of the fragment(s).
		Agarose gels are submerged in electrophoresis buffer in a horizontal electrophoresis apparatus.
	mycoplasmas.vm.iastate.edu /lab_site/methods/DNA/agarosegel2.html (497 words)

	Agarose gel electrophoresis
		Agarose gel electrophoresis separates DNA fragments according to their size.
		An electric current is used to move the DNA molecules across an agarose gel, which is a polysaccharide matrix that functions as a sort of sieve to help "catch" the molecules as they are transported by the electric current.
		Unknown DNA samples are typically run on the same gel with a "ladder." A ladder is a sample of DNA where the sizes of the bands are known.
	lifesciences.asu.edu /resources/mamajis/agarose/agarose.html (461 words)

	Reference.com/Encyclopedia/Agarose gel electrophoresis
		Agarose gel electrophoresis is a method used in biochemistry and molecular biology to separate DNA or RNA molecules by size.
		Increasing the agarose concentration of a gel reduces the migration speed and enables separation of smaller DNA molecules.
		The vast majority of agarose gels used in modern biochemistry and molecular biology are prepared and run horizontally.
	www.reference.com /browse/wiki/Agarose_gel (1460 words)

	Agarose Gel Electrophoresis
		Agarose, which is extracted from seaweed, is a linear polymer whose basic structure is shown in figure fig:agar.
		Agarose gels are cast by melting the agarose in the presence of the desired buffer until a clear, transparent solution is achieved.
		Upon hardening, the agarose forms a matrix, the density of which is determined by the concentration of the agarose.
	www.uni-graz.at /~binder/thesis/final/node41.html (872 words)

	Notice
		Agarose is a high quality purified agar that is used to separate molecules in the range of thousands to hundreds of bases of DNA.
		It is important to notice that agarose gels are not polymerized molecules, rather they are agarose molecules that have absorbed the water and buffer forming a matrix.
		This denaturing agarose gels are used to separate RNA for northern analysis.
	www.upct.es /~genetica/prv2/electrophoresis.html (586 words)

	Agarose Gel Electrophoresis of Food Coloring Dyes
		Agarose, a highly purified form of agar, is a polymer that is made entirely of sugar molecules.
		Therefore, we are going to use agarose gel electrophoresis to pull dye molecules through an agarose gel and separate them according to size and positive charge.
		Boil the mixture to melt the agarose by heating it in a microwave.
	www.biol.sc.edu /~elygen/AgGel.html (874 words)

	Agarose Gel Electrophoresis with Dyes- Teacher Guide
		While the agarose is solidifying in the gel tray, the students can practice using the micropipet with the practice dye.
		Agarose is the gel matrix used to separate molecules, such as DNA and dyes, during electrophoresis.
		To keep the agarose liquified (for example, during several biology classes), store the bottle or flask of agarose in a hot water bath between 600 and 700C.
	biotech.biology.arizona.edu /labs/Electrophoresis_dyes_teach.html (1477 words)

	Gel electrophoresis
		Two types of agarose from the same manufacturer (both in use in this laboratory) were compared for their efficiency in separating the multiplex PCR products (Fig.
		Although NuSieve agarose is much more expensive, it provides some cost reduction by requiring less amount of agarose for the same separation power and by requiring less amount of separation time.
		Agarose gels can be run at various voltages, depending on the separation desired and the available time.
	info.med.yale.edu /genetics/ward/tavi/p15.html (1013 words)

	Agarose
		This agarose is suitable for Northern and Southern blotting.
		This is am intermediate melting and gelling point agarose with a high resolution and high transparency, even at concentrations as high as 5%.
		RESolve Low agaroses have greater sieving properties than standard agaroses and are recommended for DNA/RNA separations greater than 1000bp as well as preparative protein electrophoresis.
	www.geneflow.co.uk /products/prodinfo/agarose.asp (495 words)

	Agarose Summary
		Both agar and agarose are able to liquefy when heated sufficiently, and both return to a gel state upon cooling.
		Agarose is obtained by purification of the agar.
		Sheets of agarose gels are readily prepared by pouring the warm, liquid solution into a mold, and are frequently used in molecular biology for the separation of large molecules by electrophoresis.
	www.bookrags.com /Agarose (835 words)

	LAB MANUAL, EXERCISE #22: DNA Video & Agarose Gels (Site not responding. Last check: 2007-11-07)
		Since molecules free in solution easily disperse, agarose is used to restrain the diffusion of the molecules being separated.
		When electrical current is applied, the molecules in the wells migrate through the agarose gel towards the respective electrodes while remaining in a relatively compact band.
		If proper controls are not run, if the samples are allowed to become contaminated or degraded (or tampered with), if the personnel carrying out the tests and their interpretation are not adequately trained professionals or if record-keeping is not of the highest quality, the data must be suspect.
	www.towson.edu /~gekpenyo/315lab22.htm (1693 words)

	Agarose Gel Electrophoresis of DNA (Site not responding. Last check: 2007-11-07)
		Agarose I™ is a standard melting/gelling agarose, suitable for routine nucleic acid and protein analytical and preparative applications.
		Agarose SFR™ is a high resolution sieving agarose with unsurpassed clarity.
		This agarose is suitable for the analysis of AFLP’s (Amplified Fragment Length Polymorphisms), STR’s (Short Tandem Repeats) and tetranucleotide repeats.
	www.midsci.com /docs/opt/agarose.html?products_Description=Agarose (244 words)

	Casting and Loading an Agarose Gel
		Agarose is a very pure form of agar, which is actually made from a kind of seaweed.
		To prepare or "cast" an agarose gel, agarose powder is mixed with buffer, heated, and poured into a casting or gel tray containing a comb.
		Agarose can be disposed of in everyday trash, and buffer can be disposed of in the sink (should be washed down with water to prevent salt build-up in sinks).
	nerds.unl.edu /pages/preser/sec/skills/gel.html (1777 words)

	Electrophoresis I
		Agarose gel electrophoresis is a method for separating and visualizing DNA fragments produced by restriction digestion of DNA.
		The fragments are separated by charge and size by forcing them to move through a agarose gel matrix which is subjected to an electric field.
		Agarose is a marine colloid purified from algae.
	a32.lehman.cuny.edu /molbio_course/agarose1.htm (458 words)

	Molecular Research Center Super Agarose for gel electrophoresis
		Super Agarose is stable at room temperature for at least one year after the date of purchase.
		Use repeated pipetting of the electrophoretic buffer to wash the residual agarose from the pipette.
		Mix the diluted suspension and heat it to boiling in a microwave or on a hot plate with stirring, until all the agarose is melted and dissolved to form a clear homogenous solution.
	www.mrcgene.com /SuperAgarose.htm (686 words)

	Agarose gel electrophoresis (basic method)
		Agarose gel electrophoresis is the easiest and commonest way of separating and analyzing DNA.
		The volume of agarose required for a minigel is around 30–50mL, for a larger gel it may be 250mL.
		While the agarose is cooling, prepare the gel tank ready, on a level surface.
	www.methodbook.net /dna/agarogel.html (2136 words)

	Millipore Catalogue - DNA Extraction from Agarose Gels
		The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).
		Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
		Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.
	www.millipore.com /catalogue.nsf/docs/C7487 (258 words)

	Promega - FAQs
		Agarose Digesting Enzyme is a unique agarose-digesting enzyme developed by Promega for simple and quantitative recovery of intact DNA or RNA from agarose gels.
		Agarose Digesting Enzyme performs equally well in TAE (pH 7.3, 7.8 or 8.3) and TBE (pH 8.3) and performs adequately in 20mM phosphate and MOPS buffers across the pH range 6.5–8.5.
		Agarose Digesting Enzyme is used according to suggested procedures, >95% of the nucleic acid of interest will be recovered from an agarose gel slice.
	www.promega.com /faq/agarace.html (458 words)

	[No title]
		The intensity of this band changed inversely to the template band, indicating competition during the PCR amplification between the authentic and synthetic cDNA molecules.
		This transition is not observed with chromatin depleted of histones H1-H5 (Fig.3b).
		Taking into account that histones H1-H5 are dissociated from chromatin when the NaCl concentration is 0.6 M(34), these results indicate that the presence of histones H1-H5 is necessary in order to maintain the integrity of the structures that produce the typical electrophoretic bands of native chromatin.
	www.lycos.com /info/agarose-gel-electrophoresis--miscellaneous.html (678 words)

	Protocol for Agarose Gel Preparation
		To prepare the highest quality agarose gels of any percentage,an additional 3-5min of boiling after completely melting the agarose is recommended.
		A significant amount of water evaporates during this procedure and therefore restoring of the initial weight (in step 5) is required to obtain the desired percentage gel.
		Low percentage LM agarose gels can be solidified at +4°C. Immerse the gel into the desired electrophoresis buffer and load samples.
	www.fermentas.com /techinfo/electrophoresis/pagarosegels.htm (286 words)

	Agarose Gel Electrophoresis of RNA
		This denaturing agarose gel method for RNA electrophoresis is modified from "Current Protocols in Molecular Biology", Section 4.9 (Ausubel et al., eds.).
		Native agarose gel electrophoresis may be sufficient to judge the integrity and overall quality of a total RNA preparation by inspection of the 28S and 18S rRNA bands.
		Add 10X native agarose gel loading buffer (15% ficoll, 0.25% xylene cyanol, 0.25% bromophenol blue) to the RNA samples to a final concentration of 1X.
	www.ambion.com /techlib/append/supp/rna_gel.html (964 words)

	LabScientific, Inc.
		Agarose-MB Grade-High Gel Strength is a standard gelling temperature, high gel strength agarose that resolves DNA fragments greater than 1,000 bp This agarose is designed for preparative DNA electrophoresis.
		Agarose gels (2-4%) approximate the resolution of (4-8%) polyacrylamide gels.
		Agarose 3:1 is a molecular biology grade, standard melting temperature agarose that yields strong gels for fine resolution of small DNA, RNA and PCR products less than 1 Kb.
	www.labscientific.com /BiochemicalReagents_Agarose.asp (308 words)

	Agarose Gel DNA Quantitation
		Students will be looking at agarose gels, or photographs of agarose gels, to compare a known DNA sample (Lambda/HindIII) to an unknown sample to determine the quantity and size of an unknown DNA sample.
		Gel quantitation is a simple technique used by research scientists to quickly estimate the quantity and size of DNA fragments in a particular sample run on an agarose gel stained with ethidum bromide.
		This can be done by adding 0.8 grams of agarose to 99.2 ml of 1X TBE buffer and microwaving on medium low for 2-3 minutes or until clear(make sure not to seal the container while microwaving).
	www.accessexcellence.org /AE/AEC/AEF/1996/brown_dna.html (1429 words)

	Efficient removal of PCR inhibitors using agarose-embedded DNA preparations -- Moreira 26 (13): 3309 -- Nucleic Acids ...
		The use of agarose blocks containing embedded DNA improves the PCR amplification from templates naturally contaminated with polysaccharides or humic acids, two powerful PCR inhibitors.
		Large DNAs remain trapped within the agarose blocks, whereas cell debris and contaminants are free to diffuse during lysis and washing steps.
		Agarose blocks were extracted from moulds and the embedded cells were lysed by overnight incubation at 50°C in a 0.01 M Tris, 0.5 M EDTA pH 9.2, 1% Lauroyl sarcosine and 2 mg/ml proteinase K solution (~1 ml of solution per agarose block).
	nar.oxfordjournals.org /cgi/content/full/26/13/3309 (1862 words)

Try your search on: Qwika (all wikis)