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Topic: Agarose gel electrophoresis


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DNA

In the News (Fri 19 Jul 19)

  
  Gel electrophoresis - Wikipedia, the free encyclopedia
Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point.
Gel electrophoresis is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules prior to use of other methods such as mass spectrometry, PCR, cloning, DNA sequencing, or immuno-blotting for further characterization.
Gel electrophoresis of proteins is usually done in an SDS polyacrylamide gel (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), by native gel electrophoresis, or by 2-D electrophoresis.
en.wikipedia.org /wiki/Gel_electrophoresis   (951 words)

  
 Agarose gel electrophoresis   (Site not responding. Last check: 2007-11-06)
Agarose gel electrophoresis (2) is employed to check the progression of a restriction enzyme digestion, to quickly determine the yield and purity of a DNA isolation or PCR reaction, and to size fractionate DNA molecules, which then could be eluted from the gel.
Although 0.7% agarose gels typically are used, in cases where the accurate size fractionation of DNA molecules smaller than 1 kb is required, a 1, 1.5, or 2% agarose gel is prepared, depending on the expected size(s) of the fragment(s).
Agarose gels are submerged in electrophoresis buffer in a horizontal electrophoresis apparatus.
mycoplasmas.vm.iastate.edu /lab_site/methods/DNA/agarosegel2.html   (497 words)

  
 Agarose gel electrophoresis - Wikipedia, the free encyclopedia
Agarose gel electrophoresis is a method used in molecular biology to separate DNA strands by size, and to determine the size of the separated strands by comparison to strands of known length.
In the case of DNA-based gel electrophoresis, an electric field is used to push negatively charged DNA molecules through a gel matrix.
DNA-based gel electrophoresis can be used for the separation of DNA fragments of 50 base pairs up to several megabases (millions of bases).
en.wikipedia.org /wiki/Agarose_gel_electrophoresis   (1256 words)

  
 Gel Electrophoresis of DNA   (Site not responding. Last check: 2007-11-06)
Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules.
Purified agarose is in powdered form, and is insoluble in water (or buffer) at room temperature.
The more agarose is dissolved in the boiling water, the firmer the gel will be.
www.life.uiuc.edu /molbio/geldigest/electro.html   (390 words)

  
 Agarose gel electrophoresis (basic method)
The volume of agarose required for a minigel is around 30–50mL, for a larger gel it may be 250mL.
I load gels from right to left with the wells facing me. This is because gels are published, by convention, as if the wells were at the top and the DNA had run down the page.
Some gel holders are not UV transparent so you have to carefully place the gel onto the glass surface of the light-box.
www.methodbook.net /dna/agarogel.html   (2136 words)

  
 Experiment 11b
In Experiment 6, it was stated that the mobility of a molecule under the influence of an electric field "is determined by its charge, its formula weight, the pore size of the matrix material and the strength of the electric field".
Agarose is used as the matrix in DNA electrophoresis because it can be used to form much larger pores than polyacrylamide.
The lower the percentage of agarose or polyacrylamide, the larger the pores.
www-class.unl.edu /bioc433/experiment11b.htm   (1541 words)

  
 Electrophoresis
Agarose gel electrophoresis is an easy way to separate DNA fragments by their sizes and visualize them.
The technique of electrophoresis is based on the fact that DNA is negatively charged at neutral pH due to its phosphate backbone.
These gels are visualized on a U.V. trans-illuminator by staining the DNA with a fluorescent dye (ethidium bromide).
faculty.plattsburgh.edu /donald.slish/Electrophoresis.html   (1251 words)

  
 Agarose Gel Electrophoresis of DNA
Agarose I™ is a standard melting/gelling agarose, suitable for routine nucleic acid and protein analytical and preparative applications.
Agarose SFR™ is a high resolution sieving agarose with unsurpassed clarity.
This agarose is suitable for the analysis of AFLP’s (Amplified Fragment Length Polymorphisms), STR’s (Short Tandem Repeats) and tetranucleotide repeats.
www.midsci.com /docs/opt/agarose.html?products_Description=Agarose   (244 words)

  
 [No title]
Portions of the reactions are mixed with gel loading dye and loaded into a well of a polyacrylamide gel and electrophoresed.
The gel percentage and electrophoresis conditions varied depending on the sizes of the DNA molecules of interest.
After electrophoresis, the gel is dried and exposed to x-ray film, as discussed below for radiolabeled DNA sequencing.
www.genome.ou.edu /protocol_book/protocol_partI.html   (3791 words)

  
 Agarose Gel Electrophoresis of DNA
During this laboratory you will use agarose gel electrophoresis to separate DNA fragments which have been generated by digestion of your plasmid DNA with restriction endonucleases.
While the gel is cooling, prepare the DNA samples by adding 1 uL of tracking dye to 5 uL of each restriction digest.
Run the gel until the tracking dye is approximately 3/4 the way across the gel.
csm.jmu.edu /biology/courses/bio480_580/mblab/agarose.html   (656 words)

  
 Agarose Gel Electrophoresis
Fill an electrophoresis chamber with 1X running buffer until the gel is covered by a couple of millimeters.
Electrophoresis the gel at 11 volts overnight or 100 volts for a couple of hours.
Agarose % Range of separation 0.3 60 - 5 kb 0.6 20 - 1 kb 0.7 10 - 0.8 kb 0.9 7 - 0.5 kb 1.2 6 - 0.4 kb 1.5 4 - 0.2 kb 2.0 3 - 0.1 kb
wheat.pw.usda.gov /~lazo/methods/lazo/agarose.html   (339 words)

  
 Agarose Gel Electrophoresis of DNA
The image to the right shows migration of a set of DNA fragments in three concentrations of agarose, all of which were in the same gel tray and electrophoresed at the same voltage and for identical times.
Agarose gels, as discussed above provide the most commonly-used means of isolating and purifying fragments of DNA, which is a prerequisite for building any type of recombinant DNA molecule.
Isolation of DNA from Agarose and Polyacrilamide Gels
arbl.cvmbs.colostate.edu /hbooks/genetics/biotech/gels/agardna.html   (1131 words)

  
 Agarose Gel Electrophoresis with Dyes- Student Handout
In this laboratory you will use gel electrophoresis to separate molecules present in different dyes, and you will see some of the applications of electrophoresis.
When the agarose gel is hard, take out the stoppers and pour TAE solution over your gel so that the gel casting tray is completely covered.
Carefully remove casting tray with gel and draw a picture of your gel to scale by measuring the distance traveled by the bands in the dyes.
biotech.biology.arizona.edu /labs/Electrophoresis_dyes_stude.html   (417 words)

  
 Agarose gel electrophoresis   (Site not responding. Last check: 2007-11-06)
In agarose gel electrophoresis the DNA is forced to move through a sieve of molecular proportions that is made of agarose (agarose can be used to thicken soup, but it is expensive!).
The place in the gel that the DNA migrated to is observable under ultraviolet light when the current is turned off and the gel is stained with ethidium bromide.
The adjoining picture shows an agarose gel being loaded with DNA and then being run by the application of an electric current.
www.biochem.uwo.ca /community/molbio/agarose.html   (166 words)

  
 Agarose gel electrophoresis   (Site not responding. Last check: 2007-11-06)
Place casting platform with well former sideways in gel stand where you wish to pour the gel (preferably in the 4'C cold room).
For a 1.2% gel, add 1.2g high purity, wide range Agarose per 100 ml to be made.
When gel solidifies, turn gel and tray to proper position and fill gel stand with 0.5X TBE so that it covers gel completely.
www.unc.edu /~fbottone/protocol/electro.html   (292 words)

  
 Beginning Molecular Biology Laboratory Manual
Prepare gel tray by sealing ends with tape or other custom-made dam.
Place comb in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray
To run, gently remove the comb, place tray in electrophoresis chamber, and cover (just until wells are submerged) with electrophoresis buffer (the same buffer used to prepare the agarose).
www.research.umbc.edu /~jwolf/m6.htm   (266 words)

  
 Agarose Gel Electrophoresis with Food Color- Student Handout
In this laboratory you will use gel electrophoresis to separate molecules present in different food color mixtures.
Pour until the gel comes close to the top of the gel deck, but do not overfill.
Draw a diagram on your gel sheet of the banding pattern of the samples.
biotech.biology.arizona.edu /labs/Electrophoresis_fdclr_stud.html   (269 words)

  
 Virtual Lab: Agarose Electrophoresis   (Site not responding. Last check: 2007-11-06)
The program running below is a simulation of an agarose gel electrophoresis setup that allows you to understand how restriction enzyme digests are analyzed.
To get the best appreciation for this technique, it would be best to review the sections on Agarose Gel Electrophoresis of DNA and Restriction Mapping if you have not done so already.
One thing you will observe in the simulation is also important in the real world: if two fragments of DNA differ in size by only a small amount (say less than 100 bp for fragments larger than about 1 kb), they will run sufficiently close to one another to appear as a single band.
arbl.cvmbs.colostate.edu /hbooks/genetics/biotech/gels/virgel.html   (334 words)

  
 Agarose Gel Electrophoresis   (Site not responding. Last check: 2007-11-06)
Pour 1X TAE into the horizontal electrophoresis chamber (p.
Place gel in apparatus, and add 1X TAE to completely cover wells.
agarose gel, sometimes 85V for small cast, 106V for large cast).
www-swiss.ai.mit.edu /~rweiss/exp-sig/node61.html   (76 words)

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