[IVANOVA (E.P.) and MIKHAILOV (V.V.): A new family, Alteromonadaceae fam.
IVANOVA (E.P.), FLAVIER (S.) and CHRISTEN (R.): Phylogenetic relationships among marine Alteromonas -like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam.
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Comparative 16S rRNA gene sequence analysis revealed that strain BH030046(T) formed a distinct lineage in the family Alteromonadaceae and was distinguished from its closest related genera Alteromonas (91.4-94.8 %), Aestuariibacter (92.1-93.5 %) and Glaciecola (92.1-93.5 %) on the basis of low 16S rRNA gene sequence similarities.
Physiological and biochemical data also showed that the isolate was different from members of these three genera.
On the basis of physiological and molecular properties, strain BH030046(T) (=KCTC 12239(T)=DSM 16280(T)) represents a novel genus and species in the family Alteromonadaceae, for which the name Salinimonas chungwhensis gen.
The comparison of physico-chemical and biological parameters revealed significant differences between the sampling sites and led to the supposition of the salterns of Lanzarote being more promising for the detection of new hydrolases with outstanding extreme properties for biotechnological applications than the sites near La Baule.
223 enzyme-positive strains from Lanzarote were identified as members of the following taxonomic groups, typical for hypersaline habitats: Halomonadaceae (69 strains), Pseudomonadaceae (50 strains), Vibrionaceae (47 strains), Bacillaceae (27 strains), Alteromonadaceae (6 strains), and Archeae (12 strains).
Several of the tested strains seem to represent new species.
Emphasis will be placed on newly described species and organism that are most likely to be encountered in the clinical laboratory.
This session will cover the clinically relevant members of the families Pseudomonadaceae, Burkholderiaceae, Comamonadaceae, Caulobacteraceae, Xanthomonadaceae, Sphingomonadaceae, Oceanospirillaceae, Alteromonadaceae, Oxalobacteraceae and Methylobacteriaceae.
Aids to identification and tips on key morphologic and biochemical properties will be presented that can be used to supplement or confirm the identification obtained using commercial manual and automated test systems.
Bacteria of the genus Shewanella are gram-negative bacilli, facultatively anaerobic, belonging to the family Alteromonadaceae ( 25), that are widely distributed in marine and freshwater environments ( 23).
Shewanella algae is a bacterial species recently identified ( 23, 24) that is closely related to Shewanella putrefaciens (formerly Pseudomonas putrefaciens) ( 25).
In this study, we begin a characterization of the lux operon in a less well-studied weakly luminous bacterium Shewanella hanedai.
S. hanedai (family Alteromonadaceae) grows by non-fermentative (oxidative) metabolism and is only weakly luminescent, distinguishing it from the luminous bacteria in the family Vibrionacea.
As a first step in characterization of structural and functional aspects of S. hanedai's luciferase, we have cloned a 743 bp luxA amplicon, determined its nucleotide sequence, and evaluated its relationship to other luxA amplicons/genes.
