
	Where results make sense

Topic: Antisense therapy

Related Topics

Gene therapy

Protein engineering

In the News (Thu 31 Dec 09)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	Antisense Therapy: An Overview
		However, the primary theme of antisense therapy currently being explored for oncology purposes is the inhibition of translation of a targeted protein through complementary oligonucleotide binding to target mRNA.
		At present, the main focus of antisense therapy in oncology involves the use of approximately 20 nucleotides (oligonucleotide) synthesized to be complementary to the specific “sense” (5’ to 3’orientation) mRNA sequence responsible for coding of the targeted protein.
		Antisense properties provide a fast and convenient platform for recognizing which genes are responsible for the production of particular proteins and their possible role in biological systems.
	professional.cancerconsultants.com /current_oncology.aspx?id=29920 (1818 words)

	Gene therapy - Wikipedia, the free encyclopedia
		Gene therapy is the insertion of genes into an individual's cells and tissues to treat a disease, and hereditary diseases in particular.
		Antisense therapy is not strictly a form of gene therapy, but is often lumped together with them.
		Gene therapy trials to treat severe combined immunodeficiency (SCID) were halted or restricted in the USA when leukemia was reported in three of eleven patients treated in the French Therapy X-linked SCID (XSCID) gene therapy trial.
	en.wikipedia.org /wiki/Gene_therapy (1223 words)

	Antisense Therapy Prevents Burkitt's Lymphoma In Mice (Site not responding. Last check: 2007-10-11)
		D, have used a treatment called antisense therapy to prevent the development of Burkitt's lymphoma in laboratory mice, showing that this protection is aided by the mouse's own immunity.
		The scientists think that this strategy--using antisense therapy while at the same time revving the immune system--may also work in human patients who have relapsed after chemotherapy effectively halted their disease.
		In the lymphoma experiment, antisense DNAs were made to bind to RNA messages of the c-myc cancer gene so that they can no longer generate excess myc proteins that lead to uncontrolled blood-cell proliferation and cancer.
	www.eurekalert.org /pub_releases/1998-08/TJU-ATPB-040898.php (878 words)

	Kimmel Cancer Center: Antisense Therapy
		D, have used a treatment called antisense therapy to prevent the development of Burkitt’s lymphoma in laboratory mice, showing that this protection is aided by the mouse’s own immunity.
		The scientists think that this strategy--using antisense therapy while at the same time revving the immune system--may also work in human patients who have relapsed after chemotherapy effectively halted their disease.
		In his newly published book, "Clinical Trials of Genetic Therapy with Antisense DNA and DNA Vectors," he and his coauthors describe various preclinical and clinical studies in the United States and other countries.
	www.kcc.tju.edu /clinical/kccnews/AntisenseTherapy.htm (965 words)

	Gene therapy
		Gene therapy is the insertion of genes into an individual's cells and tissuess to treat a disease, and hereditary diseases in particular.
		In theory it is possible to transform either somatic cells (most cells of the body) or cells of the germline (such as stem cells, sperm and eggs).
		Gene therapy trials to treat severe combined immunodeficiency (SCID) were halted when leukemia was reported in several of the patients.
	www.sciencedaily.com /encyclopedia/gene_therapy (1476 words)

	Hospital Practice: Antisense Therapy (Site not responding. Last check: 2007-10-11)
		One idea is to engineer antisense capable of interacting with a specific stretch of the DNA double helix, thereby creating a triplex (a triple-strand helix) within a gene or in one of its control elements.
		Antisense designed to target messenger RNA may succeed by supporting the binding of RNase H, a nuclear enzyme that cleaves the messenger.
		To refer to antisense with effects unrelated to the span of genetic code they were intended specifically to recognize, the term aptamer has been coined.
	www.hosppract.com /genetics/9909mmc.htm (3439 words)

	GENE THERAPY: TREATMENTS (Site not responding. Last check: 2007-10-11)
		It has been suggested by many researchers that antisense therapy, being able to suppress the expression of these specific genes in glioma cells, could provide a powerful approach to treating GB as well as other malignant diseases.
		The main findings from the preliminary studies suggest that antisense therapy, with the aim to impair the expression of genes that cause tumours, could be a potential treatment for glioblastomas.
		Immunogene therapy is characterized by the induction of immune responses against the tumour; it is based on the use of recombinant DNA constructs that express cytokines.
	dragon.zoo.utoronto.ca /~jlm2001/J01T0701C/gttreat.htm (4476 words)

	Breast Cancer: Antisense Therapy May Enhance Effects Of Chemotherapy, December 11, 2001 (Site not responding. Last check: 2007-10-11)
		Antisense therapy is a relatively new way of attacking cancer cells.
		"Antisense is of particular value because you don't have to worry about p53 status, so it can potentially be used for all types of tumors," says Dr. Ruiwen Zhang, associate professor of pharmacology and toxicology at UAB and a senior author of the paper.
		By using antisense therapy to sensitize the cancer cells, it is possible to treat a patient longer or use a lower dose."
	www.obgyn.net /newsrx/womens_health-Breast_Cancer-20011211-3.asp (462 words)

	ACS :: Antisense Therapy Helps Melanoma Patients
		A new DNA-based treatment called antisense therapy has eliminated tumors in one patient with advanced melanoma – the most dangerous type of skin cancer – and caused tumors to shrink in five others patients when used along with a chemotherapy drug.
		Overall, the therapy was effective in 43 percent of patients and extended their lives by a third.
		The "antisense" strand can prevent the expression of the gene encoded by its strand-mate when fragments of it attach to some sense strand genes and prevent them from producing their proteins.
	www.cancer.org /docroot/NWS/content/NWS_1_1x_Antisense_Therapy_Helps_Melanoma_Patients.asp (682 words)

	Antisense Nucleotides Summary - Antisense Nucleotides Information
		Antisense nucleotides are strings of RNA or DNA that are complementary to "sense" strands of nucleotides.
		Antisense RNA is currently being investigated as a human therapy for certain forms of cancer.
		One advantage of using antisense therapy in treating infectious diseases such as virus infections is that it can be tailored to the particular strain in circulation, and then modified as the virus mutates.
	www.bookrags.com /sciences/genetics/antisense-nucleotides-gen-01.html (756 words)

	Antisense therapy - Wikipedia, the free encyclopedia
		Antisense therapy is a theoretical form of treatment for genetic disorders.
		When the genetic sequence of a particular gene is known to be causative of a particular disease, it is possible to synthesize a strand of nucleic acid (DNA, RNA or a chemical analogue) that will bind to the messenger RNA produced by that gene, effectively turning that gene "off".
		Most potential therapies have not yet produced significant clinical results, though one antisense drug, Formivirsen (marketed as Vitravene), has been approved by the US edit]
	en.wikipedia.org /wiki/Antisense_therapy (167 words)

	BioMedNet News and Comment (Site not responding. Last check: 2007-10-11)
		Successful oligonucleotide design is critical in antisense therapy, a pharmacologic method that uses sequence-specific nucleic acid drugs to target and degrade deleterious mRNA.
		For such therapies to be effective, the complementary target sequences must be available for hybridization and not be sequestered in secondary structure.
		Antisense activity did not improve for sequences predicted to be inactive.
	www.nasw.org /users/ajacobson/WCP_antisense.html (464 words)

	VEGF antisense therapy inhibits tumor growth and improves survival in experimental pancreatic cancer.
		VEGF antisense therapy inhibits tumor growth and improves survival in experimental pancreatic cancer.
		METHODS: In vitro: VEGF in cell culture supernatant of pancreatic cancer cells (AsPC-1, poorly differentiated; HPAF-2, moderately differentiated) was assessed by enzyme-linked immunosorbent assay.
		AS-3 therapy normalizes plasma VEGF and decreases neoangiogenesis, thereby reducing tumor growth and metastasis and improving survival.
	pancreaticcancer.researchtoday.net /archive/2/1/484.htm (372 words)

	Antisense Therapy for Cancer
		Antisense therapy works at the genetic level to prevent mutated or overactive genes from directing the synthesis of proteins that are causing cancer.
		Antisense drugs have a more selective action, and they have the potential to be more effective and less toxic than conventional drugs.
		Oligo means “a few”, and it has been found that an ideal length for an antisense molecule is from 13 to 30 nucleotides.
	www.suite101.com /article.cfm/new_cancer_treatments/60326 (444 words)

	Antisense Oligodeoxynucleotide Technology: Potential Use for the Treatment of Malignant Brain Tumors
		The number of additional potential targets for ODN therapy of glioma cells is extremely large, including genes coding for other examples of (1) growth factors and their receptors, (2) cellular proteases, kinases, and second messengers, (3) proto-oncogenes, and (4) factors and proteins important in cell cycle control and apoptosis, to identify but a few categories.
		Antisense expression of protein kinase C alpha inhibits the growth and tumorigenicity of human glioblastoma cells.
		Antisense oligodeoxynucleotide attenuates in vivo expression of c-fos in the paraventricular hypothalamic nucleus of the rat brain.
	www.moffitt.usf.edu /pubs/ccj/v5n2/article7.html (4322 words)

	antisense therapy
		antisense therapy The in vivo treatment of a genetic disease by blocking translation of a protein with a DNA or an RNA sequence that is complementary to a specific mRNA.(From Glossary of Biotechnology for Food and Agriculture)
		PSA responses (reductions of greater than 50%), seven disease stabilizations and one disease progression on this regimen of antisense therapy targeting the R2...
		Therapies that have been specially studied include, immunotherapy, gene therapy and antisense therapy.
	www.mongabay.com /igapo/biotech/antisense_therapy.html (217 words)

	Chemotherapy Foundation Symposium and Online Education Program - Mount Sinai School of Medicine (Site not responding. Last check: 2007-10-11)
		Thus, the cytotoxic effects of current therapy might be dramatically enhanced by reducing Bcl-2 expression prior to therapy, as well as by preventing its subsequent over-expression that is induced by such treatment.
		Antisense is a well-established technology for deciphering the precise function of individual proteins, and it represents a novel form of human therapeutics.
		Thus, antisense offers an opportunity to knock out a specific protein that may be critical in the biology of disease without altering any other protein, thus affording very high specificity, selectivity, and minimal toxicity.
	www.mssm.edu /tcf/archives/symposiumxviii/abstracts/1110004a_c_abs.shtml (1033 words)

	Antisense Therapy Makes Perfect Sense for Duchenne’s Muscular Dystrophy
		The treatment relies on antisense technology, the delivery of small pieces of synthetic DNA or RNA that block the action of mutated gene segments.
		The goal of antisense therapy in Duchenne’s muscular dystrophy is to bypass the mutation responsible for the inadequate dystrophin expression that eventually leads to muscle wasting and replacement with adipose and connective tissue.
		Antisense therapy restored normal dystrophin expression in about 20% of the mice’s muscle fibers and increased their muscle strength to about 70% of normal, effects that appeared quickly and persisted for up to two months.
	www.neurologyreviews.com /sep03/nr_sep03_sense.html (696 words)

	The Future of Hypertension Therapy: Sense, Antisense, or Nonsense? -- Pachori et al. 37 (2): 357 -- Hypertension (Site not responding. Last check: 2007-10-11)
		Antisense inhibition of AT receptor mRNA and angiotensinogen mRNA in the brain of spontaneously hypertensive rats reduces hypertension of neurogenic origin.
		Intravenous angiotensinogen antisense in AAV-based vector decreases hypertension.
		Antisense inhibition of hypertension in the spontaneously hypertensive rat.
	hyper.ahajournals.org /cgi/content/full/37/2/357 (4619 words)

	City of Hope :: Urology Department
		City of Hope researchers are developing gene therapy approaches that may eventually be used to treat a number of malignancies, including prostate cancer.
		In antisense therapy, pieces of genetically engineered materials that are complementary to the abnormal “messenger” material produced by the targeted gene are used to block the gene from producing its harmful protein product.
		Ultimately, it is hoped that gene therapy will fulfill its promise as an anti-cancer therapy that is more effective than the standard drug and radiation treatments available today, and less likely to be associated with unwanted side effects.
	www.cityofhope.org /urology/geneTherapy.asp (265 words)

	Antisense approaches to cancer gene therapy.
		Recent advances in the use of oligodeoxynucleotide and plasmid-derived RNA as antisense agents of special relevance to cancer gene therapy are summarized with emphasis on agents and systems which have lead to clinical trials and/or regression of established tumors in animal model systems.
		Transformed cell lines bearing plasmids and viruses designed for the transcription of antisense RNA have the advantage that they can be characterized thoroughly and the effects of antisense RNA on target gene expression and phenotype can be studied easily in vivo.
		The importance of examination of antisense effects in syngeneic and immunocompetent hosts is illustrated by studies of insulin-like growth factor and insulin-like growth factor receptor where tumor regression and protection against tumor formation have been observed for particular cell types in defined settings.
	www.aegis.com /aidsline/1995/nov/M95B0806.html (400 words)

	DG DISPATCH - AACR: Clinical Response Seen with Synthetic Antisense-based Therapy in Advanced Melanoma
		The antisense therapy targets the bcl-2 gene, Dr. Jansen said.
		The antisense protein -- a short strand of synthetic DNA -- interferes with the bcl-2 gene, keeping it from producing the shield, he said.
		He said that while there is a low threshold for effectiveness of the antisense molecule, doctors have yet to find an upper dose limit.
	www.docguide.com /dg.nsf/PrintPrint/B57E918D64D7CA2B852568B8006E6151 (578 words)

	New Approaches to Gene Therapy
		Triple-helix-forming oligonucleotide (pronounced AHL-ih-go-NOOK-leo-tide) gene therapy targets the DNA sequence of a mutated gene to prevent its transcription.
		Antisense gene therapy aims to turn off a mutated gene in a cell by targeting the mRNA transcripts copied from the gene.
		Like antisense, ribozyme (pronounced RYE-bo-ZYME) gene therapy aims to turn off a mutated gene in a cell by targeting the mRNA transcripts copied from the gene.
	gslc.genetics.utah.edu /units/genetherapy/gtapproaches (665 words)

	Antisense Therapy (AT) Blocks Cancer Growth
		A short stretch of oligonucleotides called antisense can be made to pair up with mRNA made from the PKC alpha gene.
		Fortunately, PKC alpha kinase in cancer cells is more sensitive to antisense treatment than in normal cells.
		An ambulatory pump provided continuous intravenous infusion of ISIS 3521, a 20-base phosphothiolate oligonucleotide which is complementary to the 3'-untranslated region of mRNA for human PKC alpha.
	www.meds.com /conrad/asco97/sikic.html (457 words)

	Is antisense gene therapy a step in the right direction in the control of hypertension? -- Raizada et al. 277 (2): 423 ... (Site not responding. Last check: 2007-10-11)
		Antisense gene therapy in the long-term control of hypertension.
		Retrovirus-mediated transfer of an angiotensin type 1 receptor (AT -R) antisense sequence decreases AT RS and angiotensin action in astroglia and neuronal cells in primary culture.
		Reduction of cold-induced hypertension by antisense oligodeoxynucleotides to angiotensinogen mRNA and AT receptor mRNA in brain and blood.
	ajpheart.physiology.org /cgi/content/full/277/2/H423 (4426 words)

	TGF-beta Antisense Gene Therapy (Site not responding. Last check: 2007-10-11)
		The antisense DNA segments were incorporated into the cell genome using electroporation, a process in which cells undergo an electric shock, opening the cell membrane and allowing the new DNA into the cytoplasm.
		By using a vector for introduction of the antisense TGF-, the DNA must replicate independently and as such does not incorporate into the genome, causing the scientist to use another method as a back up.
		IL-2 is capable of this since it, as a retrovirus, carries its own reverse transcriptase, allowing it to transcribe itself into the DNA of the cell.
	student.biology.arizona.edu /honors96/group15/methods.htm (442 words)

Try your search on: Qwika (all wikis)