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Topic: Arabinose

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In the News (Thu 23 May 19)

  Properties of tartaric acid...
The mean arabinose concentration in the urine samples of males with autism was over five times that of the normal male controls and the median value six times that of the normal male controls.
Elevated protein-bound arabinose has been found in the serum proteins of schizophrenics (21) and in children with conduct disorders(22) and alteration of protein function by arabinose might be another mechanism by which arabinose might effect biochemical processes in autism and other diseases.
Arabinose may also be formed from the breakdown of the sugar glucose (23) and antioxidants such as glutathione may inhibit this conversion(24).
www.greatplainslaboratory.com /book/bk5sect2.html   (2222 words)

 PLP 428: Lecture notes: lecture 25   (Site not responding. Last check: 2007-10-13)
coli, the arabinose operon is responsible for the ability of the cell to use the sugar arabinose as a carbon source.
Arabinose is a five carbon sugar that is converted into D-xylulose-5-phosphate, an intermediate in the Pentose phosphate pat hway.
Arabinose is not as efficient a carbon source as glucose, so you might expect (and you would be correct) that the cAMP/CRP regulatory system is required for the positive regulation of the arabinose catabolic operon (ara operon).
latin.arizona.edu /~mgen/micgen_98/Lect22/Lect_22.htm   (730 words)

 TnAraOut, A transposon-based approach to identify and characterize essential bacterial genes - Nature Biotechnology
Arabinose was removed from overnight cultures and cultures were diluted 1:50,000 into six different arabinose concentrations as described in the Experimental protocol.
However, in the absence of arabinose there was a range of responses, with some insertion mutants severely reduced in their ability to compete with the tester strain in liquid media (Table 1).
Support for this conclusion comes from the observation that the growth defect of these mutants in absence of arabinose is partially alleviated by culturing them in partially spent medium in which a wt strain was previously grown (Table 2).
www.nature.com /nbt/journal/v18/n7/full/nbt0700_740.html   (4226 words)

 Discussion   (Site not responding. Last check: 2007-10-13)
This is because MC4100 is arabinose sensitive and its mutated araD gene produces a nonfunctional gene product which disrupts the arabinose pathway and leads to the build-up of ribulose 5-phosphate, a cell toxin.
Another possible pattern, white on arabinose, white on lactose and red on arabinose + lactose plates, suggests that insertion occurs on the A or B gene.
The arabinose pathway is disrupted, but on an arabinose+lactose plate, the arabinose +C gene product will turn on the promoter and allow the β-galactosidase to be produced.
userpages.umbc.edu /~jtai1/discussion.htm   (610 words)

The fungal pathway for utilization of arabinose consists of five enzymes, and the dehydrogenase enzyme responsible for the reduction of arabinose to arabinitol has been characterized in Saccharomyces cerevisiae.
Thus, not only is there no evidence that arabinose is produced by any strain of yeast, the extant evidence favors the conclusion that any available evidence favors the conclusion that any available arabinose in their environment would be consumed by yeast.
Arabinose is one of the most abundant pentoses in nature and it is present in various hemicellulosic and pectic plant polysaccharides, such as heteroglycanes (e.
www.greatvistachemicals.com /proteins-sugars-nucleotides/l-arabinose.html   (485 words)

 Formal for Genetics   (Site not responding. Last check: 2007-10-13)
Through the selection of ampicillin resistant lysogens and arabinose resistant lysogens, it was discovered that the frequency of arabinose resistant lysogens to ampicillin lysogens showed a higher occurrence of ampicillin resistant colonies.
The arabinose operon is positively regulated by the Ara c gene to turn on the BAD structural genes in the presence of arabinose through self regulation of the Ara C gene.
Arabinose resistance occurs when Mud1 inserts into either the Ara A, Ara B or Ara C gene of the arabinose pathway.
userpages.umbc.edu /~singhsa1   (2145 words)

 Arabinose, pentosidine and protein crosslinks
The aldehyde group of arabinose can react with the extra amino chemical group(called an epsilon amino group) of an amino acid called lysine that is present in a wide variety of proteins.
I have found that urine arabinose is elevated in some cases of Alzheimer's disease and have received a report of a favorable response from antifungal therapy to treat Alzheimer's disease from a woman with a child with autism and a father with Alzheimer's disease.
Mechanism of autoxidative glycosylation: identification of glyoxal and arabinose as intermediates in the autoxidative modification of proteins by glucose.
www.greatplainslaboratory.com /book/bk5sect3.html   (1603 words)

 Transcriptional Activation of ydeA, Which Encodes a Member of the Major Facilitator Superfamily, Interferes with ...
arabinose uptake was mediated by AraE or AraFGH.
The properties of the arabinose regulon have led to the development of a family of expression plasmids that are extensively
Intracellular arabinose levels (in picomoles/0.2 ml of cells) were normalized to the optical density of the cultures.
jb.asm.org /cgi/content/full/181/7/2185   (5012 words)

 Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture -- Khlebnikov ...
Arabinose (0.02%) and IPTG (32 µg/ml) were added to the culture at time zero.
Six hours after addition of arabinose, the percentage of the population that was induced (A) and the population-averaged fluorescence (Fluorescence/OD ratio) (B) were determined.
Finally, at saturating arabinose concentrations (2%), a decline in the specific fluorescence was observed.
jb.asm.org /cgi/content/full/182/24/7029?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&searchid=1128011094156_2603&stored_search=&FIRSTINDEX=0&volume=182&firstpage=7029&journalcode=jb   (3084 words)

 Contribution of Gene Loss to the Pathogenic Evolution of Burkholderia pseudomallei and Burkholderia mallei -- Moore et ...
[TTSS], bsaN and bsaP; the araC gene of the arabinose operon;
The arabinose assimilation operon is present in the chromosome of B.
PCR amplification of the araA gene and the arabinose assimilation operon from B.
iai.asm.org /cgi/content/full/72/7/4172   (5753 words)

 AraC dimer, complexed with L-arabinose
Arabinose Operon Regulatory Protein, AraC complexed with L-Arabinose.
The arabinose binding protein, one of many transport proteins in E. coli.
This monomer binds the arabinose in its center, and serves as transport
www.ucmb.ulb.ac.be /~jean/BioInformatic/Session98-99/Yu.html   (346 words)

 The Arabinose Operon
Englrsberg did all the proper genetic analysis and came to the conclusion that expression of the arabinose operon was regulated in a positive manner.
Regulation of the arabinose operon is, clearly, much more complex than the lactose operon.
When arabinose is absent, there is no need to express the structural genes.
www.mun.ca /biochem/courses/3107/Lectures/Topics/Ara_operon.html   (376 words)

 Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis -- ...
or in the presence of the inducer arabinose and in repressing
increase of arabinose and consequently an increase of the metabolic
In conclusion, CCR of arabinose catabolism by glucose and glycerol
mic.sgmjournals.org /cgi/content/full/149/9/2345   (5134 words)

 Development and Validation of a Whole-Cell Inhibition Assay for Bacterial Methionine Aminopeptidase by Surface-Enhanced ...
arabinose were stained with a fluorescent viability stain.
Parental or MAPr strains were grown in the presence or absence of arabinose and stained with a fluorescence viability stain as described in Materials and Methods.
Cultures of parental and MAPr strains after the dilution of arabinose were collected and prepared for SELDI-TOF MS as described in Materials and Methods.
aac.asm.org /cgi/content/full/49/8/3428   (3920 words)

 JHU CMDB Graduate Program
Arabinose C. Protein: Regulation of the Arabinose Operon in vitro, J. Greenblatt and R. Schleif, Nature New Biology 233, 166-170 1971.
The Isolation and Characterization of Plaque-forming Arabinose Transducing Bacteriophage, J. Lis and R. Schleif, J. Mol.
The Regulatory Region of the L-arabinose Operon: Its Isolation on a 1000 Base-pair Fragment from DNA Heteroduplexes, J. Lis and R. Schleif, J. Mol.
www.jhu.edu /cmdb/Faculty/Schleif/pub.htm   (2027 words)

 [No title]
The control of the Arabinose operon is much like the lactose operon since both control metabolism of sugars.
One difference is that the araC protein also acts as an activator when bound to arabinose which is necessary to start transcription.
Predict the phenotypes (expression of araA or araB in the presence or absence of glucose or arabinose) for these strains: Arabinose presentArabinose absent Arabinose and Glucose presentaraB activityaraA activityaraB activityaraA activityaraB activityaraA activityaraC+ araB+ araA+ araC- araB+ araA- araC+ araB- araA-/F’araB+araA+araC+ araO- araB+ araA+/ F’ araO+ araB- araA- 3.
www.uvm.edu /~dstratto/bcor101/problems10.doc   (385 words)

 Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides -- de Vries and Visser 65 (4): 497 -- ...
Arabinose is connected to the backbone of xylan via an
O-5 of terminal arabinose residues (324, 343, 397).
In cereals, cinnamic acids (mainly ferulic acid) are ester-linked to arabinose residues in arabinoxylan in the primary cell
mmbr.asm.org /cgi/content/full/65/4/497   (6218 words)

 Information on the herb aloe vera.
The anthraquinones contained in aloe, which has the purging and laxative action on the digestive system includes aloin (barbaloin), isobarloin, anthranol, aloetic acid, anthracene, ester of cinnamic acid, aloe-emodin, emodin, chrysophanoic acid, ethereal oil as well as resistannol.
It contains various saccharides including glycoprotein, mucopolysaccharide and polysaccharides such as galactose, xylose, arabinose, acetylated mannose as well as the remarkable acemannan.
The conversion of fatty acids to prostaglandins are rare but the major unsaturated fatty acid, gamma-linolenic acid can be converted to 1-series prostaglandins and have a very effective influence on reducing inflammation, allergy as well as helping platelet aggregation (blood clotting at wound sites) as well as wound healing.
www.ageless.co.za /herb-aloe.htm   (1376 words)

 Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents ...
Expression after induction by 1.33 mM arabinose also was examined in individual cells by fluorescence microscopy.
of arabinose, the majority of the cells are faintly fluorescent.
After the lag in induction at low arabinose concentrations, the level of GFP approaches a plateau that is substantially lower
www.pnas.org /cgi/content/full/94/15/8168   (4322 words)

 Cells Can Respond to Changes in Their Environments
Chemicals that could pass into cells, either by diffusion through the cell membrane or by the action of transport proteins, and could bind directly to proteins inside the cell and modulate their activities.
An example is the use of the sugar arabinose by the bacterium Escherichia coli (Figure 2.19).
However, if arabinose is their only source of carbon, E.
www.ncbi.nlm.nih.gov /books/bv.fcgi?rid=stryer.section.223   (1817 words)

 Site-Specific Recombination-Based Genetic System for Reporting Transient or Low-Level Gene Expression -- Casavant et ...
arabinose was released from the seeds of several legumes and
Arabinose is a component of seedling exudates (31) and
Heyn, A. A gas chromatographic analysis of the release of arabinose from coleoptile cell walls under the influence of auxin and dextranase preparations.
aem.asm.org /cgi/content/full/68/7/3588   (5439 words)

 Pfam 19.0 : AraC_binding   (Site not responding. Last check: 2007-10-13)
The crystal structure of the arabinose-binding and dimerization domain of the Escherchia coli gene regulatory protein AraC was determined in the presence andabsence of L-arabinose.
The arabinose-bound molecule shows that the protein adopts an unusual fold, binding sugar within a beta barrel and completely burying the arabinose with the amino-terminal arm of the protein.
Dimer contacts in the presence of arabinose are mediated by an antiparallel coiled-coil.
pfam.wustl.edu /cgi-bin/getdesc?name=AraC_binding   (177 words)

 Health, Autistic Spectrum Disorder
He noted that whenever the Nystatin was stopped the symptoms would worsen and the arabinose levels would increase and when it was restarted the symptoms would decline again along with the arabinose.
He concluded that arabinose probably has some neurotoxic effect and that it is necessary to treat autistic children for long periods of time (often over a year) in order to prevent this toxic problem.
Both HPHPA and arabinose, as well as other toxic substances, may be measured by getting a urine organic acid test.
www.springboard4health.com /notebook/health_autism2.html   (5952 words)

 [No title]
Currently ethanol is produced by fermentation of glucose from the starch portion of the corn kernel, however nearly 11% of the kernel is represented in the fiber portion.
Complete arabinose uptake studies to determine if transport is taking place.
Explore classical mutagenesis (Creation of a new trait in an organism) techniques for strain modification to utilize arabinose.
www.ncga.com /research/pdfs/PFS_C_FF.doc   (1241 words)

 Chemical Compound Outline (Part I)
It also prevents the formation of ice crystals in frozen deserts and adds viscosity to catsup.
Complex polysaccharides composed of sugars other than glucose (primarily arabinose, fucose, galactose, mannose, rhamnose and xylose).
Many different species of plants in a variety of families produce sticky saps composed of gums.
waynesword.palomar.edu /chemid1.htm   (15712 words)

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