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Topic: Ascorbate peroxidase

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Haem peroxidases Fungal, plant and bacterial haem peroxidases (yeast cytochrome c peroxidase, ascorbate peroxidase, bacterial catalase­peroxidases, lignin peroxidase, manganese peroxidase, secretory plant peroxidases). Peroxidases are found in bacteria, fungi, plants and animals. On the basis of sequence similarity, haem peroxidases can be categorised into two superfamilies: fungal, plant and bacterial peroxidases form one superfamily [3] and animal enzymes form another [4]. metallo.scripps.edu /PROMISE/PEROXIDASES.html   (271 words)

Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases -- Zámocky 271 (16): 3297 -- FEBS Journal cerevisiae[7] and ascorbate peroxidase (APX) from Pisum sativum[8] database) is discernible in all the peroxidases analysed. are 100% conserved in all the peroxidases analysed. content.febsjournal.org /cgi/content/full/271/16/3297   (5294 words)

Regulation and function of ascorbate peroxidase isoenzymes -- Shigeoka et al. 53 (372): 1305 -- Journal of Experimental Botany Ascorbate peroxidase: a prominent membrane protein in oilseed glyoxysomes. Amako K, Chen GX, Asada K. Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isoenzymes of ascorbate peroxidase in plants. Chen G-X, Asada K. Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. jxb.oupjournals.org /cgi/content/full/53/372/1305   (5294 words)

Increasing vitamin C content of plants through enhanced ascorbate recycling -- Chen et al. 100 (6): 3525 -- Proceedings of the National Academy of Sciences Abbreviations: AsA, ascorbic acid • MDHA, monodehydroascorbate • MDHAR, MDHA reductase • DHA, dehydroascorbate • DHAR, DHA reductase • GSH, reduced glutathione • GSSG, oxidized glutathione • GR, glutathione reductase • APX, ascorbate peroxidase • GLDH, Effect of sucrose on ascorbate level and expression of genes involved in the ascorbate biosynthesis and recycling pathway in harvested broccoli florets the ascorbate redox state in both tobacco and maize. www.pnas.org /cgi/content/abstract/100/6/3525   (5294 words)

Table of contents for Library of Congress control number 98028908 Other Class I Peroxidases: Ascorbate Peroxidase and Bacterial Catalase-Peroxidases. Horseradish Peroxidase I: Ligand Binding, Redox Potentials, Formation of Its Compounds, and Some of Their Reactions. Class II Peroxidases: Lignin, Manganese, and Coprinus cinereus Peroxidases. www.loc.gov /catdir/toc/onix03/98028908.html   (201 words)

Citrulline and DRIP-1 Protein (ArgE Homologue) in Drought Tolerance of Wild Watermelon -- YOKOTA et al. 89 (7): 825 -- Annals of Botany Miyake C, Asada K. Inactivation mechanism of ascorbate peroxidase at low concentrations of ascorbate; hydrogen peroxide decomposes Compound I of ascorbate peroxidase. Miyake C, Yokota A. Cyclic flow of electrons within PSII in thylakoid membranes. Akashi K, Miyake C, Yokota A. Citrulline, a novel compatible solute in drought-tolerant wild watermelon leaves, is an efficient hydroxyl radical-scavenger. aob.oxfordjournals.org /cgi/content/full/89/7/825   (4218 words)

Low temperature-induced changes in the distribution of H2O2 and antioxidants between the bundle sheath and mesophyll cells of maize leaves -- Pastori et al. 51 (342): 107 -- Journal of Experimental Botany APX, ascorbate peroxidase; ASC, ascorbate; BS, bundle sheath; CAT, catalase; CuZn-SOD, CuZn-containing superoxide dismutase; DHA, dehydroascorbate; DHAR, dehydroascorbate reductase; DTT, dithiothreitol; Fe-SOD, Fe-containing superoxide dismutase; GR, glutathione reductase; M, mesophyll; MDHAR, monodehydroascorbate reductase; Mn-SOD, Mn-containing superoxide dismutase; PPFD, photosynthetic photon flux density; PSII, photosystem II; SOD, superoxide dismutase. mesophyll extracts of plants grown at 15 °C. Ascorbate and Bundle sheath proteins are more sensitive to oxidative damage than those of the mesophyll in maize leaves exposed to paraquat or low temperatures. jexbot.oupjournals.org /cgi/content/full/51/342/107   (4218 words)

David Dalton, Reed College Biology Department Effectiveness of ascorbate and ascorbate peroxidase in promoting nitrogen fixation in model systems. Effects of exogenous application and stem infusion of ascorbate on soybean ( Glycine max) root nodules. Dalton, D.A., Sasha Kramer*, Nico Azios*, Suzanne Fusaro*, Elizabeth Cahill*, and Christina Kennedy. academic.reed.edu /biology/professors/ddalton/index.html   (4218 words)

Symbiotic Hydra express a plant-like peroxidase gene during oogenesis -- Habetha and Bosch 208 (11): 2157 -- Journal of Experimental Biology RT PCR (lanes 1,3,5) and genomic PCR amplification (lanes 2,4,6) of Chlorella vulgaris 211-11b and Hydra viridis ascorbate peroxidase. the Chlorella and Hydra viridis ascorbate peroxidase genes, In the Cnidarian Hydra viridis, symbiotic algae of the jeb.biologists.org /cgi/content/full/208/11/2157   (4302 words)

Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves www.uni-hohenheim.de /i3ve/00068900/19044041.htm   (4302 words)

BioMatNet Item: AIR1-CT92-0205 - Engineering Stress Tolerance in Maize (ESTIM) To this end, transgenic maize plants will be generated which over express in the chloroplasts both a superoxide dismutase and an enzyme implicated in hydrogen peroxide scavenging (ascorbate peroxidase, glutathione peroxidase, catalase, glutathione reductase, or glutathione synthetase). The damage caused by low-temperature stress occurs primarly in the chloroplasts, leading to an early inhibition of photosynthesis, photobleaching of pigments, and considerable losses in quality and yield. Since maize originates from tropical regions it is particularly sensitive to low-temperature stress, explaining why stress tolerance has become a major selection criteria in maize breeding programmes. www.biomatnet.org /secure/Air/S296.htm   (4302 words)

PubMed nucleotide query REFERENCE 1 (bases 1 to 1524) AUTHORS Jespersen,H.M., Kjfrsgerd,I.V., Oestergaard,L. and Welinder,K.G. TITLE From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure, function and evolution of seven types of ascorbate peroxidase JOURNAL Biochem. ORGANISM Arabidopsis thaliana Eukaryotae; Viridiplantae; Charophyta/Embryophyta group; Embryophyta; vascular plants; seed plants; Magnoliophyta; Magnoliopsida; Capparales; Brassicaceae; Arabidopsis. genetics.biol.ttu.edu /Agbiotech_files/Lab%203/AT%20sAPX%20seq%20ori.htm   (4302 words)

Zonal Changes in Ascorbate and Hydrogen Peroxide Contents, Peroxidase, and Ascorbate-Related Enzyme Activities in Onion Roots -- del Carmen Córdoba-Pedregosa et al. 131 (2): 697 -- PLANT PHYSIOLOGY Zonal Changes in Ascorbate and Hydrogen Peroxide Contents, Peroxidase, and Ascorbate-Related Enzyme Activities in Onion Roots -- del Carmen Córdoba-Pedregosa et al. Zonal Changes in Ascorbate and Hydrogen Peroxide Contents, Peroxidase, and Ascorbate-Related Enzyme Activities in Onion Roots our knowledge, this is the first exhaustive report on zonal differences www.plantphysiol.org /cgi/content/full/131/2/697   (6360 words)

Antioxidative Effects of Melatonin in Protection Against Cellular Damage Caused by Ionizing Radiation -- Karbownik and Reiter 225 (1): 9 -- Experimental Biology and Medicine Pablos MI, Reiter RJ, Ortiz GG, Agapito MT, Chuang JI, Sewerynek E. Rhythms of glutathione peroxidase and glutathione reductase in the brain of chick and their inhibition by light. Both melatonin and a putative nuclear melatonin receptor agonist CGP 52608 stimulate glutathione peroxidase and glutathione reductase activities in mouse brain in vivo. Melatonin, a mediator of electron transfer and repair reactions, acts synergistically with the chain-breaking antioxidants ascorbate, trolox, and glutathione. www.ebmonline.org /cgi/content/full/225/1/9   (8046 words)

Iron-Regulated Expression of a Cytosolic Ascorbate Peroxidase Encoded by the APX1 Gene in Arabidopsis Seedlings -- Fourcroy et al. 134 (2): 605 -- PLANT PHYSIOLOGY Iron-Regulated Expression of a Cytosolic Ascorbate Peroxidase Encoded by the APX1 Gene in Arabidopsis Seedlings -- Fourcroy et al. Articles by Fourcroy, P. Articles by Briat, J.-F. Iron-Regulated Expression of a Cytosolic Ascorbate Peroxidase Encoded by the APX1 Gene in Arabidopsis Seedlings Articles by Fourcroy, P. Articles by Briat, J.-F. Agricola www.plantphysiol.org /cgi/content/abstract/134/2/605   (8046 words)

ARS Publication request: Ascorbate Peroxidase Is Sorted Indirectly to the Glyoxysome Boundary Membrane Via a Subdomain of the Endoplasmic Reticulum In this study, the intracellular sorting of pAPX to the boundary membrane of glyoxysomes was examined in BY-2 cells. Both transiently-expressed, epitope-tagged pAPX and a chloramphenicol acetyltransferase (CAT) pAPX fusion protein (CAT-pAPX) were localized in vivo to glyoxysomes and to a subdomain of the endoplasmic reticulum (ER) termed herein as 'glyoxysomal ER' (gER). Collectively, these results suggest that pAPX, a tail-anchored (Ncytosol-Cmatrix) integral membrane protein, is targeted to glyoxysomes via a pre-glyoxysomal compartment that is derived from a portion of the ER. www.ars.usda.gov /research/publications/publications.htm?SEQ_NO_115=99881&pf=1   (292 words)

Peroxisomal Ascorbate Peroxidase Resides within a Subdomain of Rough Endoplasmic Reticulum in Wild-Type Arabidopsis Cells -- Lisenbee et al. 132 (2): 870 -- PLANT PHYSIOLOGY AtAPX in peroxisomal and rough ER vesicle membranes. Electron micrograph of a portion of an Arabidopsis suspension cell illustrating profiles of rough ER (er), peroxisomes (p), mitochondria (m), plastids with starch grains (pl), and Golgi bodies (g) dispersed throughout the cytoplasm. Intact peroxisomes and rough ER vesicles from the pellet fraction (Fig. www.plantphysiol.org /cgi/content/full/132/2/870   (7688 words)

Glutathione It is evident that glutathione's reducing power is used in conjunction with ascorbate and other antioxidants to protect the entire spectrum of biomolecules, to help regulate their function, and to facilitate the survival and optimal performance of the cell as a living unit. Reduced levels of GSH peroxidase have been found in the platelets of patients with acute myocardial infarction, Glanzmann's thrombasthenia, and the Hermansky-Pudlak syndrome characterized by dysfunctional platelets. Reducing power is also the key to the multiple actions of GSH at the molecular, cellular, and tissue levels, and to its effectiveness as a systemic antitoxin. www.thorne.com /altmedrev/fulltext/glut.html   (7688 words)

Effects of methyl viologen (paraquat) stress on SODs of Arabidopsis seedlings: protein, enzyme and transcript responses M methyl viologen on plastid Fe SOD, cytoplasmic Cu/Zn SOD, cytoplasmic ascorbate peroxidase protein populations and on Mn, Fe and Cu/Zn SOD enzyme activities was investigated in Arabidopsis leaves over a 50 hour post-treatment period. An increase in the amounts of proteins for all three enzymes was observed by western analysis whereas a decrease in Fe SOD and an increase in Mn SOD enzyme activity was observed on activity gels. Effects of methyl viologen (paraquat) stress on SODs of Arabidopsis seedlings: protein, enzyme and transcript responses abstracts.aspb.org /pb1997/65/1887.shtml   (250 words)

Location and effects of long-term NaCl stress on superoxide dismutase and ascorbate peroxidase isoenzymes of pea (Pisum sativum cv. Puget) chloroplasts -- Gómez et al. 55 (394): 119 -- Journal of Experimental Botany Miyake C, Asada K. Inactivation mechanism of ascorbate peroxidase at low concentrations of ascorbate; hydrogen peroxide descomposes compound I of ascorbate peroxidase. The percentage of activity was calculated by dividing the specific activity of each isoenzyme in the thylakoids or stroma by the total activity, considered as the sum of its specific activity in solubilized thylakoids plus stroma, as described in the Materials and methods. Stroma and thylakoid samples applied to the gels contained 200 µg of proteins. jxb.oxfordjournals.org /cgi/content/full/55/394/119   (6719 words)

Mechanism of peroxidase actions for salicylic acid-induced generation of active oxygen species and an increase in cytosolic calcium in tobacco cell suspension culture -- Kawano and Muto 51 (345): 685 -- Journal of Experimental Botany Miyake C, Sano S, Asada K. A new assay of ascorbate peroxidase using the coupled system with monodehydroascorbate radical reductase. Heber U, Miyake C, Mano J, Ohno C, Asada K. Monodehydroascorbate radical detected by electron paramagnetic resonance spectrometry is a sensitive probe of oxidative stress in intact leaves. Kawano T, Sahashi N, Takahashi K, Uozumi N, Muto S. Salicylic acid induces extracellular generation of superoxide followed by an increase in cytosolic calcium ion in tobacco suspension culture: the earliest events in salicylic acid signal transduction. jxb.oxfordjournals.org /cgi/content/full/51/345/685   (4423 words)

article-14745826-changes-leucine-transport-activity-chironomus-riparius-larvae.htm Transport inhibition observed in larvae exposed to potassium dichromate was accompanied by changes in ascorbate peroxidase and dehydroascorbate reductase activities, whereas those exposed to fenitrothion displayed an increase in transaminase activity. Exposure to potassium dichromate and fenitrothion caused a dose- and time-dependent inhibition of leucine uptake. The effect of sublethal concentrations of potassium dichromate and fenitrothion on sodium-leucine cotransport in brush border membrane vesicles from Chironomus riparius larvae has been investigated. www.find-health-articles.com /article-14745826-changes-leucine-transport-activity-chironomus-riparius-larvae.htm   (4423 words)

Post-Transcriptional Suppression of Cytosolic Ascorbate Peroxidase Expression during Pathogen-Induced Programmed Cell Death in Tobacco -- Mittler et al. 10 (3): 461 -- THE PLANT CELL PCD that occurs during the HR is accompanied by an increase PCD may be untranslatable due to specific or nonspecific modifications. PCD, the translation of the mRNA encoding cAPX is inhibited. www.plantcell.org /cgi/content/full/10/3/461   (4423 words)

Peroxisomal Membrane Ascorbate Peroxidase Is Sorted to a Membranous Network That Resembles a Subdomain of the Endoplasmic Reticulum -- Mullen et al. 11 (11): 2167 -- THE PLANT CELL Nontransformed cells, or cells transiently transformed with HA-pAPX by biolistic bombardment, were fixed in formaldehyde (~20 hr after bombardment), treated with pectolyase Y-23, incubated in 0.3% Triton X-100 (to permeabilize all cellular membranes), and then incubated with primary and dye-conjugated secondary antibodies. Cells transiently transformed with HA-pAPX (except [D] and [E]) were fixed (except [E]) in formaldehyde (~20 hr after bombardment), treated with pectolyase Y-23, incubated in 0.3% Triton X-100, and then incubated with primary and dye-conjugated secondary antibodies. Transiently transformed cells were fixed in formaldehyde (~20 hr after bombardment), treated with pectolyase Y-23, incubated in 0.3% Triton X-100, and then incubated with primary and dye-conjugated secondary antibodies. www.plantcell.org /cgi/content/full/11/11/2167   (8265 words)

Heat Stress- and Heat Shock Transcription Factor-Dependent Expression and Activity of Ascorbate Peroxidase in Arabidopsis -- Panchuk et al. 129 (2): 838 -- PLANT PHYSIOLOGY APX activity and AsA/DHA content were measured for at least four batches of plants in four replicates each. APX expression and the interdependence of heat stress and oxidative APX activity decreased to 31% in WT and 35% in HSF3 transgenic www.plantphysiol.org /cgi/content/full/129/2/838   (8039 words)

Plant Gene Register PGR98-131 Kubo A, Saji H, Tanaka K, Kondo N (1993a) Genomic DNA structure of a gene encoding cytosolic ascorbate peroxidase from Arabidopsis thaliana. Kubo A, Sano T, Saji H, Tanaka K, Kondo N, Tanaka K (1993b) Primary structure and properties of glutathione reductase from Arabidopsis thaliana. Glutathione reductase (GR; EC 1.6.4.2), which is a member of the flavoprotein disulfide oxidoreductase family, catalyzes the conversion of GSSG to GSH using NADPH as an electron donor. www.tarweed.com /pgr/PGR98-131.html   (1055 words)

Chronic Fatigue Syndrome Anderson R. Ascorbate-mediated stimulation of neutrophil motility and lymphocyte transformation by inhibition of the peroxidase/H2O2/halide system in vitro and in vivo. Gray and Martinovic found changes in the ratio of biologically active EFA metabolites such as would be expected as an exaggeration of normal physiological response to excessive or prolonged stress. Warren G, McKendrick M, Peet M. The role of essential fatty acids in chronic fatigue syndrome. www.thorne.com /altmedrev/fulltext/fatigue5-2.html   (6234 words)

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