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Topic: Aspergillus niger

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	Encyclopedia: Aspergillus niger (Site not responding. Last check: 2007-10-29)
		Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus.
		Aspergillus niger is less likely to cause disease than some other Aspergillus species, but if large amounts of the spores are breathed in, the serious lung disease aspergillosis can occur.
		In culture, Aspergillus niger is used in the manufacture of citric acid (E330), gluconic acid (E574) and the enzyme alpha-galactosidase (Beano®, Bean-zyme™) and has been assessed as acceptable for daily intake by the World Health Organisation.
	www.nationmaster.com /encyclopedia/Aspergillus-niger (748 words)

	Aspergillus niger vacuolar aspartyl protease - Patent 5674728 (Site not responding. Last check: 2007-10-29)
		The invention further concerns a novel Aspergillus mutant strain defective in a protease of the aspartic proteinase-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.
		Conidiospores of Aspergillus niger strain N400 are inoculated in 200 ml minimal medium to a final spore density of 10.sup.6 spores/ml and shaken in 11 Erlenmeyers for 24 h at 28.degree.
		The terminator of the A. niger pyruvate kinase gene is amplified from pGW1100 (DSM 5747) by PCR using oligonucleotides 5 and 6.
	www.freepatentsonline.com /5674728.html (14430 words)

	Aspergillus niger mutants - US Patent 5318895 (Site not responding. Last check: 2007-10-29)
		Disclosed are new Aspergillus niger mutants which can be used for identifying active FK-506/ rapamycin type, or cyclosporin A type inununosuppressants in a broth, natural product extract, or composition exhibiting inununosuppressant activity.
		The Aspergillus niger microorganisms are currently on deposit with the American Type Culture Collection, 12301 Parklawn Drive in Rockville, Maryland as indicated by the herein described ATCC Nos., and in the Merck Culture Collection at Rahway, New Jersey as indicated by the respective MF Nos.
		The starting parent strain is Aspergillus niger, ATCC 6275 (NRRL 334) in the Merck culture collection as MF 442 and used in the antifungal screening panel.
	www.patentstorm.us /patents/5318895.html (2896 words)

	Aspergillus niger Antibodies
		Aspergillus precipitins are seen in 90% of patients with fungus balls, 70% of patients with allergic bronchopulmonary aspergillosis, and less often in patient's with invasive aspergillosis.
		The demonstration of Aspergillus antigen in serum is extremely sensitive and specific for the diagnosis of aspergillosis, but is not yet widely available.
		Brummund W, Resnick A, Fink JN, et al, "Aspergillus fumigatus-Specific Antibodies in Allergic Bronchopulmonary Aspergillosis and Aspergilloma: Evidence for a Polyclonal Antibody Response," J Clin Microbiol, 1987, 25(1):5-9.
	www.labcorp.com /datasets/labcorp/html/chapter/mono/al003400.htm (222 words)

	Aspergillus niger. (Site not responding. Last check: 2007-10-29)
		This fungus is the third most common Aspergillus species associated with disease and is a very common environmental isolate.
		Aspergillus niger and Aspergillus fumigatus have been reported to cause skin diseases and are a common cause of fungal related ear infections - otomycosis.
		Aspergillus niger generates many types of secondary metabolites including malformin C and some of the naptho-y-quinones.
	www.kgainc.com /artman/publish/printer_27.shtml (110 words)

	630. Enzymes derived from Aspergillus niger (WHO Food Additives Series 22)
		niger is a contaminant of food and was not considered in the same light as those organisms regarded as normal constituents of food.
		NIGER Aspergillus niger is a contaminant of food.
		niger, and different cultural conditions might be used to prepare the various enzymes, the available toxicity data, which consist primarily of short-term feeding studies in rats and some studies in dogs, show that all the enzyme preparations tested were of a very low order of toxicity.
	www.inchem.org /documents/jecfa/jecmono/v22je04.htm (2500 words)

	384. Microbial carbohydrase (aspergillus niger) (WHO Food Additives Series 6)
		Three groups of 10 male and 10 female Charles River rats were fed 0, 5 and 10% of Aspergillus niger mycelial preparation in their diet for 13 weeks.
		Mouse, Rat, Cat Enzyme preparations from Aspergillus niger, strain "Pectolytic" produced by different cultivation methods were in several different acute and short-term studies given to a total number of 300 mice, 123 rats and 17 cats.
		The conclusion of the author is, that surface and deep culture preparations on media rich in sugars (sugar beet pulp, wheat bran) gave effects in some experimental animals after 45-750 mg/kg bw/day, of the different preparations mainly given in the drinking water.
	www.inchem.org /documents/jecfa/jecmono/v06je31.htm (920 words)

	Tom Volk's Fungus of the Month for February 1997 (Site not responding. Last check: 2007-10-29)
		Aspergillus is a member of the "deuteromycetes," or Fungi Imperfecti, which is a group (technically) reserved for fungi for which there is no known sexual state.
		Aspergillus species are very difficult to distinguish from one another without a great deal of practice.
		Aspergillus is named for a device called an aspergillum, which is used by a priest at a Catholic mass to sprinkle holy water on the faithful.
	botit.botany.wisc.edu /toms_fungi/feb97.html (844 words)

	Aspergillus/Aspergillosis Website
		The sequencing of A. niger is the first step in one of the largest industrial genome projects in Europe - integrating a number of techniques, including high throughput DNA sequencing, DNA-micro-analyses, proteomics, metabolomics and bio-informatics - which has confirmed DSM's positioning as one of Europe's leading bio-technology companies.
		The Aspergillus niger genome was sequenced by Gene Alliance.The genome was annotated by Biomax Informatics.
		The DNA sequence of Aspergillus niger is estimated to consist of approximately 30 million base pairs, which would make this study what is believed to be the largest industrial genome contract sequencing project in Europe to date.
	www.aspergillus.man.ac.uk /secure/sequence_info/genalliance.html (1104 words)

	El-Holi and Al-Delaimy. Citric acid production from whey with sugars and additives by Aspergillus niger. Afr. J. ...
		Rohr et al (1983) reported that CA can be produced by fermentation process using species of microorganisms namely Aspergillus niger, a fungus which was used commercially for the first time in 1923.
		niger from cheese whey fortified with different concentrations of sucrose, glucose, fructose, galactose, tricalcium phosphates, methanol and riboflavin in a liquid surface culture process.
		niger ATCC 9642 stock culture was reactivated and cultivated by streaking a loopfull of the culture on Petri dishes containing solidified acidified(with 10% tartaric acid) potato dextrose agar (PDA) and incubated at 25
	www.academicjournals.org /AJB/manuscripts/manuscripts2003/Octobermanuscripts2003/El-Holi/El-Holi%20and%20Al-Delaimy.htm (1275 words)

	ipedia.com: Aspergillus niger Article (Site not responding. Last check: 2007-10-29)
		It causes fl mould on certain types of fruit and vegetables.
		It is used in the manufacture of citric acid (E330), gluconic acid (E574) and the enzyme alpha-galactosidase (Beano®, Bean-zyme™) and has been assessed as acceptable for daily intake by the World Health Organisation.
		They report that the fungus is a common contaminant of food but is not pathogenic to humans.
	www.ipedia.com /aspergillus_niger.html (167 words)

	Title of Invention: Gene sequence encoding Aspergil lus niger catalase-R
		The A. niger catA gene, cloned by cross-hybridization to the yeast CTA1 gene, encodes a catalase enzyme which is induced primarily during growth on fatty acids and is presumably peroxisomal.
		Features of the A. niger strain used as a host for expression of the glucoamylase-catalase cassette include a) uridine-requiring auxotrophy, specifically a pyrG auxotrophic mutation, b) deletion of the gene encoding glucose oxidase, goxA, and (c) a methionine-requiring auxotrophy, specifically mutation which renders the cells deficient in cystathionase (metC) activity.
		The A. niger pyrG gene was isolated from pUC4XL as an EcoRI fragment having 27 bp and 16 bp of pUC4XL polylinker DNA at either end.
	www.nal.usda.gov /bic/Biotech_Patents/1994patents/05360901.html (3637 words)

	Aspergillus niger (Site not responding. Last check: 2007-10-29)
		Aspergillus niger is a fungus and one of the most commonspecies of the genus Aspergillus.It causes fl mould on certain types of fruit and vegetables.
		Although often found in thepassages of the outer ear, Aspergillus niger is not likely to cause disease.
		It is used in the manufacture of citric acid (E330), gluconic acid (E574) and the enzyme alpha-galactosidase (Beano®, Bean-zyme™) and has been assessed as acceptable for daily intake bythe World Health Organisation.
	www.therfcc.org /aspergillus-niger-61574.html (107 words)

	Aspergillus Niger Inquiry of July 02 (Site not responding. Last check: 2007-10-29)
		Aspergillus Niger or Black Mold, most likely will also be found in your carpets, drapes and linen.
		Most likely a large number of patients seen by ENTs are suffering from some form of Aspergillus Niger contamination they picked up from the heating or air-conditioning system in their home or at work.
		However, Aspergillus Niger is identifiable and it can be a very serious infection if allowed to go untreated.
	www.infostew.com /children/_children/00000008.htm (1079 words)

	Purification and properties of a 4-nitrophenylphosphatase from Aspergillus niger. (Site not responding. Last check: 2007-10-29)
		Purification and properties of a 4-nitrophenylphosphatase from Aspergillus niger.
		Purification and properties of a 4-nitrophenylphosphatase from Aspergillus niger.A small, but reproducible, amount of activity was measured using 5'-DNA phosphate as a substrate.
		A 4-nitrophenylphosphatase (EC 3.1.3.41) was identified in extracts of Aspergillus niger.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/41664.html (194 words)

	Aspergillus niger mutants - Patent 5318895 (Site not responding. Last check: 2007-10-29)
		The disc results can be observed visually, or read with a spectrophotometer to measure intensity and compared to a known standard of FK-506, rapamycin or CsA to determine the MIC.sub.50, the relative concentration of the known or unknown drug needed to inhibit 50% of the growth of the microorganism.
		Spontaneous mutants resistant to FK-506/Rapamycin were obtained by spreading 10.sup.5 /mL conidia of the parent Aspergillus niger ATCC No. 6275 on agar containing 4 g/1 yeast extract (Difco), 10 g/1 malt extract (Difco) and 4 g/1 dextrose (YME) and FK-506 at 20.mu.g/ml.
		Utilizing the above procedure, the FK-506/Rapamycin Resistant isolate, ATCC No. 74087 in conjunction with the sensitive parent, A. niger ATCC No. 6275, or with the CsA-Resistant strain, ATCC No. 74085, or both is used to detect and validate the presence in broths or any other medium of the FK-506 or rapamycin class of compounds.
	www.freepatentsonline.com /5318895.html (2653 words)

	Mannitol Is Required for Stress Tolerance in Aspergillus niger Conidiospores -- Ruijter et al. 2 (4): 690 -- Eukaryotic ...
		niger mpdA gene, encoding MPD; the disruption of the gene by
		Isolation and expression of the Aspergillus nidulans pyruvate kinase gene.
		Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans.
	ec.asm.org /cgi/content/full/2/4/690 (5616 words)

	Expression of an Aspergillus niger Phytase Gene (phyA) in Saccharomyces cerevisiae -- Han et al. 65 (5): 1915 -- ...
		Aspergillus niger phytase (EC 3.1.3.8) has been well characterized: it is an extracellular glycoprotein, and the mature
		Expression of an Aspergillus niger phytase (phyA) in Escherichia coli.
		Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger.
	aem.asm.org /cgi/content/full/65/5/1915 (3457 words)

	Mycology Online -- Aspergillus
		Aspergillus niger is one of the most common and easily identifiable species of the genus Aspergillus, with its white to yellow mat later bearing fl conidia.
		This is the third most common species associated with invasive pulmonary aspergillosis.
		A. niger may also be a common laboratory contaminant.
	www.mycology.adelaide.edu.au /Fungal_Descriptions/Hyphomycetes_(hyaline)/Aspergillus/niger.html (181 words)

	Aspergillus niger endocarditis in an immunocompetent patient: an unusual course -- Kreiss et al. 76 (892): 105 -- ...
		Aspergillus niger endocarditis in an immunocompetent patient: an unusual course
		Aspergillus is an opportunistic nosocomial fungus generally associated with a high mortality rate.
		Direct microscopy of the vegetation stained with 10% KOH-Quink revealed septate hyphae and culture grew A niger (figure 2).
	www.postgradmedj.com /cgi/content/full/76/892/105 (845 words)

	Physical characterization of the aldehyde-dehydrogenase-encoding gene of Aspergillus niger. (Site not responding. Last check: 2007-10-29)
		Physical characterization of the aldehyde-dehydrogenase-encoding gene of Aspergillus niger.
		The aa sequence of the A. niger enzyme is significantly homologous to mammalian sequences, particularly around one Cys residue, which is hypothesized to be adjacent to another Cys known to be at the active site of mammalian AldDHs.
		Sequence comparisons of the 5' region of the A. niger aldA gene to other Aspergillus genes has shown a common sequence in the 5' regions of several A. nidulans genes, all but one of which are subject to carbon catabolite repression.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/2382.html (231 words)

	Cyclic AMP-dependent protein kinase is involved in morphogenesis of Aspergillus niger -- Saudohar et al. 148 (8): 2635 ...
		Cyclic AMP-dependent protein kinase is involved in morphogenesis of Aspergillus niger -- Saudohar et al.
		a, M. A spontaneous change in the intracellular cyclic AMP level in Aspergillus niger is influenced by the sucrose concentration in the medium and by light.
		Kusters-van Someren, M. A., Harmsen, J. M., Kester, H. and Visser, J. Structure of the Aspergillus niger pelA gene and its expression in the Aspergillus niger and Aspergillus nidulans.
	mic.sgmjournals.org /cgi/content/full/148/8/2635 (4724 words)

	Expression of an Aspergillus niger Phytase Gene (phyA) in Saccharomyces cerevisiae -- Han et al. 65 (5): 1915 -- ...
		Expression of an Aspergillus niger Phytase Gene (phyA) in Saccharomyces cerevisiae -- Han et al.
		Expression of an Aspergillus niger Phytase Gene (phyA) in Saccharomyces cerevisiae
		niger phytase gene (phyA) in Saccharomyces cerevisiae and to determine
	aem.asm.org /cgi/content/abstract/65/5/1915 (403 words)

	Expression of the Caldariomyces fumago Chloroperoxidase in Aspergillus niger and Characterization of the Recombinant ...
		Expression of the Caldariomyces fumago Chloroperoxidase in Aspergillus niger and Characterization of the Recombinant Enzyme -- Conesa et al.
		The Caldariomyces fumago chloroperoxidase was successfully expressed in Aspergillus niger.
		niger strain MGG029 was transformed with a mixture of plasmids pCPO3.I-AmdS and pAB4-1.
	www.jbc.org /cgi/content/full/276/21/17635 (3917 words)

	MSN Encarta - Mold (fungi)
		Mold and mildew are commonly used interchangeably, although mold is often applied to fl, blue, green, and red fungal growths, and mildew to whitish growths.
		Black bread mold, Aspergillus niger, one of the most familiar molds, begins as a microscopic, airborne spore that germinates on contact with the moist surface of nonliving organic matter.
		It spreads rapidly, forming the mycelium (fungal body), which is made up of a fine network of filaments (hyphae).
	encarta.msn.com /encnet/refpages/refarticle.aspx?refid=761573526 (357 words)

	76A-25 Accumulation and recovery of gallic acid in a submerged culture of Aspergillus niger Aa-20 (Site not responding. Last check: 2007-10-29)
		Accumulation and recovery of gallic acid in a submerged culture of Aspergillus niger Aa-20
		The main objective of this work was to evaluate the accumulation of gallic acid in submerged cultures (SmC) of Aspergillus niger using tannic acid as sole carbon source.
		Spores harvested of A. niger, were used to inoculate the SmC.
	ift.confex.com /ift/2002/techprogram/paper_14159.htm (365 words)

	COMPARISON OF DIFFERENT METHODS FOR METABOLITE EXTRACTION FROM ASPERGILLUS NIGER MYCELIUM (Site not responding. Last check: 2007-10-29)
		Two ways of quenching metabolism and four procedures for extraction of intracellular metabolites were elaborated with the filamentous fungus Aspergillus niger.
		Amounts of pyruvate in Aspergillus niger mycelia varied from 0.5 to 2.0 mmol/g dry biomass, 2-oxoglutarate ranged between 0.2 and 0.8 mmol/g dry mycelium and malate from 3.0 to 16.0 mmol/g respectively, depending on time of cultivation.
		Extractions of Aspergillus niger mycelia by perchloric acid and by sodium hydroxide were efficient and reliable, whereas treatment with buffered boiling ethanol returned too low amounts of metabolites examined.
	acta.chem-soc.si /51/51-3-567.htm (214 words)

	BioMatNet Item: QLK3-2002-02038 - Demonstration of increased yield and productivity in selected commercial organisms by ...
		This programme will demonstrate that the transfer of specific genes from an Aspergillus niger strain, which produces citric acid with close to theoretical efficiency, into other commercial micro-organisms will enhance the pool of precursors in those organisms.
		The main objective of the proposed project is to demonstrate that the transfer of some strategic genes from Aspergillus niger, one of the most efficient industrial micro-organisms, into other commercially important micro-organisms will increase the productivity and/or yield of their bio-products.
		niger genes that enhance anapleurotic pathways, the reactions that replenish citric acid cycle intermediates.
	www.biomatnet.org /secure/FP5/S1712.htm (631 words)

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