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Topic: Assay

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In the News (Mon 19 Aug 19)

  Definition of assay - Merriam-Webster Online Dictionary
1archaic : trial, attempt2: examination and determination as to characteristics (as weight, measure, or quality)3: analysis (as of an ore or drug) to determine the presence, absence, or quantity of one or more components; also : a test used in this analysis4: a substance to be assayed; also : the tabulated result of assaying
Learn more about "assay" and related topics at Britannica.com
See a map of "assay" in the Visual Thesaurus
www.m-w.com /cgi-bin/dictionary?book=Dictionary&va=assay   (84 words)

  Comet Assay Interest Group
Single cell gel (SCG) electrophoresis or 'Comet assay' is a rapid and very sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level.
This assay has critical applications in fields of toxicology ranging from aging and clinical investigations to genetic toxicology and molecular epidemiology.
The assay is attractive because of its simplicity, sensitivity, versatility, speed, and economy, and the number of publications it spawns rises each year.
www.cometassay.com   (555 words)

  Assay - Wikipedia, the free encyclopedia
An assay is a procedure where the concentration of a component part of a mixture is determined.
There are numerous applications of an assay, such as an antigen capture assay, bioassay, competitive protein binding assay, four-point assay, immunoassay, microbiological assay, stem cell assay, and many others.
Assays are regularly utilized in scientific research laboratories.
en.wikipedia.org /wiki/Assay   (239 words)

 Enzyme assay - Wikipedia, the free encyclopedia
Enzyme assays are laboratory methods for measuring enzymatic activity.
The MTT assay, a redox assay using a tetrazolium dye as substrate is an example of a colorimetric assay.
These assays can be used to measure reactions that are impossible to assay in any other way.
en.wikipedia.org /wiki/Enzyme_assay   (1030 words)

 Fire Assay Information
Fire assaying is the quantitative determination in which a metal or metals are separated from impurities by fusion processes and weighed in order to determine the amount present in the original sample.
For fire assaying ores, usually fluxes, materials such as borax, soda, silica are added to the ore. The amounts of each chemical added to the flux mixture depends upon the elements present in the particular ore, such as sulfur and iron.
The most common unit of fire assay samples is called the Assay Ton, which is 29.166 grams of ore. A milligram (0.001) gram of gold in a sample weighing 29.166 grams gives an assay equivalent to 1 troy ounce of gold in a ton (2,000 pound) of ore. A troy ounce is 1.097 ounces.
www.mine-engineer.com /mining/assay2.htm   (532 words)

Precious metals, platinum, gold and silver, used in jewellery, silverware and other items, are assayed to test the purity of the metal.
This is done in the UK by an Assay Office.
Once an item has been assayed, it is usually hallmarked to the relevant standard.
www.ibpassociation.org /encyclopedia/Chemistry/Assay.php   (296 words)

 The Goldsmiths' Company - The London Assay Office
The testing and marking of jewellery and silverware to guarantee the precious metal content of an article has been carried out by the Goldsmiths' Company since 1300.
The process is known today as hallmarking, which originally meant 'marked at Goldsmiths' Hall'.
The techniques of sampling, testing (assaying) of the precious metal content (fineness) and marking have altered over the centuries, but the principle of providing a guarantee for manufacturers, importers, retailers, and the eventual purchaser, remains the same.
www.thegoldsmiths.co.uk /assayoffice   (187 words)

 Assay definition - Medical Dictionary definitions of popular medical terms easily defined on MedTerms
Thus, an assay may be done for example to determine the level of thyroid hormones in the blood of a person suspected of being hypothyroid (or hyperthyroid).
For example, an assay may be done of a vaccine to determine its potency.
The words "assay" and "essay" are often confounded, a cardinal sin according to some word mavens.
www.medterms.com /script/main/art.asp?articlekey=8412   (263 words)

The Clinical Ligand Assay Society cordially invites you to become an active member of the Society that promotes and educates individuals in the use of Ligand assays.
Members of the Society come from a variety of backgrounds but are unified by their special interest in ligand assays.
The Society is of a size that allows for close interaction among the members at both the national and local chapter level.
www.clas.org   (158 words)

 Free Assay Analysis
Let us analyze a parallel-line assay or parallel-logistics assay on the basis of your measurement data with PLA 2.0.
Please use the form below or send us your assay data by fax at +49 (6106) 7701029 or by e-mail at support@bioassay.de.
If your assay configuration is not reflected by this form, please
www.bioassay.de /index.php?id=assay_analysis   (197 words)

 Cellular Cytotoxicity Assay
This colorimetric assay kit can be used to monitor the release of a cytosolic enzyme from cultured cells that suffer damage or death as the result of in vitro exposure to toxins.
One is for cellular cytotoxicity assays in which the lysis of target cells by effector lymphocytes is monitored.
The second protocol is for chemotoxicity assays in which the potential toxic effects of chemical agents are examined by monitoring their toxicity for indicator cell lines upon challenge with dilutions of the chemical agent.
www.oxfordbiomed.com /lk100cyto.html   (213 words)

 Tutorial: Assay: Miniaturized Cellular Assays   (Site not responding. Last check: )
For agonist assays, cell suspension (25 l) was dispensed onto compound (25 l) in a 384-well fl, clear-bottom plate (Greiner) with simultaneous imaging of the entire plate for 40 seconds with 10-second baseline prior to cell addition.
For antagonist assays, compound and cells were pre-mixed (25 l of each), incubated for 10 minutes, with a final addition (25 l) of agonist (EC80).
For the antagonist assay compound and cells were pre-mixed (3 l of each), incubated for 10 minutes, with a final addition (3 l) of agonist (EC80).
www.genengnews.com /current/article.aspx?cat=Tutorial&id=1320   (1195 words)

In the DISC assay, the entire contents of the cell culture are cytocentrifuged onto permanent microscope slides and differentially stained to allow discrimination of normal and neoplastic cells and living and dead cells.
Furthermore, results are compared with a database of assays which have similar technical characteristics, as the technical characteristics of these assays influence the in vitro results, along with the intrinsic drug resistance of the tumor cells.
The assay is conceptually a cell death assay, albeit one which is performed on pre-cultured (and ostensibly amplified) cells.
www.htaj.com /faq.htm   (4444 words)

 Chemical Identification vs. Assay
Assay specifications are usually very tight such as 99.0- 101.0%.
Titrations and other wet chemical assays are usually not very specific, and their use assumes the material has been correctly identified.
For example the assay methods for both sodium and potassium chloride involves measuring the chloride ion concentration using a silver nitrate titration.
www.wcaslab.com /tech/assay.htm   (330 words)

 SHE assay - Syrian Hamster Embryo Cell Transformation Assay used in toxicology   (Site not responding. Last check: )
The SHE assay is a short-term in vitro assay that assesses a substance's carcinogenic potential based on its ability to cause morphologic transformation of SHE cells.
The Syrian Hamster Embryo (SHE) assay is a short-term in vitro test designed to assess a substances carcinogenic potential based it its ability to cause morphologic transformation of SHE cells.
BioReliance's SHE assay is the latest in a series of expansions designed to enhance the range of services available to clients interested in genetic toxicology testing.
www.bioreliance.com /SHE_assay.html   (351 words)

 Extreme Drug Resistance (EDR) Assay
The Extreme Drug Resistance (EDR) Assay is an advanced laboratory test for cancer, also known as a "chemotherapy drug resistance test." This test is performed by growing a portion of the cancer tumor in the presence of different chemotherapy drugs in the laboratory.
EDR Assay results have been widely published in scientific literature and have been shown to be 99% accurate in clinical trials.
By eliminating inactive drugs from the therapy regimen, use of the EDR Assay avoids exposing patients to the toxicity of those drugs that are unlikely to be effective in treating the tumor and can help to improve the quality of life of the cancer patient during treatment.
virtualtrials.com /draassay.cfm   (833 words)

 COMET assay - single cell gel electrophoresis used in toxicology   (Site not responding. Last check: )
Comet assay, also known as single cell gel electrophoresis (SCG), is a microgel electrophoresis technique which detects DNA damage and repair in individual cells.
Comet assay can be conducted in both in vitro and in vivo test systems and is increasingly being used in genotoxic testing of industrial chemicals, agrochemicals and pharmaceuticals.
The assay can also be used to complement the information gained through the ICH Gene Tox battery of tests and be part of the submission package to regulatory agencies.
www.bioreliance.com /comet_assay.html   (475 words)

 ATCC: MTT Cell Proliferation Assay
ATCC's MTT Cell Proliferation Assay offers a quantitative, convenient method for evaluating a cell population's response to external factors, whether it be an increase in cell growth, no effect, or a decrease in growth due to necrosis or apoptosis.
Assays are run in a 96-well plate and read with a microtitre plate reader, allowing high-throughput handling of samples.
Because the MTT Cell Proliferation Assay requires less cell manipulation than [3H]thymidine incorporation assays (no cell harvesting or medium changes are necessary), the possibility of error is reduced and the standard deviation values are lower.
www.atcc.org /Products/MttCell.cfm   (895 words)

 CDC - Rapid Antigen-Capture Assay To Detect West Nile Virus in Dead Corvids
Sensitivity and specificity of the antigen-capture assay applied to oropharyngeal and cloacal swabs from corvids were calculated by using the results of TaqMan RT-PCR assays on swab or tissue samples as the “true” outcome (6).
The sensitivity and specificity of the antigen-capture assay, when oropharyngeal swabs from crows collected in 2002 were used, were 83.3% and 95.8%, respectively, indicating that this assay is acceptable for use as a field detection test in a WNV surveillance program.
The poorer performance of the VecTest assay in noncorvids may be related to species-specific differences (and variability) in virus titers in excretions and secretions at the time of death (3).
www.cdc.gov /ncidod/EID/vol9no11/03-0318.htm   (2923 words)

 Chemicon - Product #SGT410 - Tyrosine Kinase Activity Assay
Assaying of PTK activity allows for purification and characterization of protein tyrosine kinases, elucidation of their biological functions as well as aiding in development of specific PTK inhibitors.
Therefore, the composition of the assay buffer (buffer substance, pH, stabilizers) depends largely on the specific tyrosine kinase of interest (refer to literature) (5, 6).
The assay utilizes a synthetic biotinylated peptide substrate that can be phosphorylated by a wide range of tyrosine kinases in solution.
www.chemicon.com /browse/productdetail.asp?ProductID=SGT410   (830 words)

 Cayman Chemical | New Products | Antibodies/Enzymes/Assay Kits
The assay procedure involves cell lysis and PBMC quantification in the pellet using an intercalating substrate followed by fluorescence detection.
The assay is primarily intended for use in routine monitoring of DA levels in bivalve molluscs to comply with the regulatory MPL, but is also applicable for DA quantification in other marine matrices.
The assay utilizes arachidonoyl-1-thio-glycerol as a novel chromogenic substrate for the measurement of MGL activity.
www.caymanchem.com /app/template/NewProducts.vm/dicer/antibodiesAssayKits/a/z;jsessionid=AC6556F1C64EE5F8171F5804587E7B65.neptuneprimeworker   (1911 words)

 RFA-HG-05-001: Molecular Screening Assay Development for SCD
The assays should be suitable to be candidates for screening in the NIH supported high throughput screening (HTS) centers that will be funded under the NIH Molecular Libraries and Imaging Roadmap Initiative: http://nihroadmap.nih.gov/molecularlibraries/index.asp).
Proposals should include assay development plans that are sufficient to demonstrate reproducibility in a low-to-moderate throughput setting, i.e., tens or hundreds of compounds, and must be feasible for adaptation to an automated, high-throughput screening approach.
Although assays developed under this RFA will be eligible for consideration by the NIH screening centers, funding under this RFA does not carry a commitment by NIH to accept the assay for screening at a center.
grants1.nih.gov /grants/guide/rfa-files/RFA-HG-05-001.html   (5486 words)

 PROTOCOLS- COMET ASSAY FORUM   (Site not responding. Last check: )
This was a neutral assay in which the lysis and electrophoresis were done under neutral conditions.
The neutral assay was modified by two groups, Singh and co-workers (1988) and Olive et al (1989).
Hence, comet assay can be used to study various classes of DNA damage by controlling the conditions of the lysing and electrophoresis steps.
www.comet.itrcindia.org /protocols.htm   (710 words)

 IMAP FP PKA Assay Kit
This assay kit is designed for use in drug discovery to screen the activity of PKA in the presence of putative drug compounds.
For FP detection such binding causes a change in the rate of the molecular motion of the peptide, and results in an increase in the fluorescence polarization value observed for the fluorescein label attached at the end of the peptide (Figure 1).
This assay, unlike antibody-based kinase assays, is applicable to a wide variety of kinases without regard to the substrate peptide sequences.
www.moleculardevices.com /pages/reagents/imap_pka.html   (343 words)

 Elsevier MDL :: Company Information :: News :: Press Releases :: 2005
The flexible Assay Explorer system will streamline workflow by making it easy for biologists to add columns and associated calculations to a spreadsheet when analyzing experimental results—without having to wait for administrative help to modify a template.
The new Assay Explorer makes it possible to create and calculate a 500 × 96-well-plate HTS experiment in less than five minutes and has been shown to recalculate an existing experiment in two seconds.
Assay Explorer also integrates out-of-the-box with GraphPad Prism® for non-linear regression analysis and with a variety of other third-party statistical analysis and modeling tools.
www.mdli.com /company/news/press_releases/2005/pr_assayexplorer_8aug05.jsp   (523 words)

 HistoSoft » Bradford Assay » Bradford Assay Background
The Bradford Assay is a colorimetric assay for measuring total protein concentration in a given solution.
Within a particular range of concentrations, this proportional relationship is linear and relatively predictable; consequently, a regression curve can be derived from a series of standards and used as the basis for estimating a series of unknowns.
Besides selecting the protein standard that most closely mimics the properties of the experimental protein samples, one should adjust the protein concentrations based upon the standard stock concentration and the expected range of the protein of interest in solution.
www.histosoft.com /services/background/ba/baback.html   (1206 words)

 The Bradford Assay
The assay is monitored at 595 nm in a spectrophotometer, and thus measures the CBBG complex with the protein.
The assay does perform linearly over short concentration stretches, and this has most likely resulted in the overall conclusion that the assay is linear.
The micro assay format is designed for protein concentrations between 1-20 micrograms and the macro assay is designed for protein concentrations in the range of 20-100 micrograms.
www-class.unl.edu /biochem/protein_assay/bradford_assay.htm   (960 words)

 Assay Validation, NIAID Division of HIV Vaccine Site
Assay validation is a process of determining the suitability of a given laboratory method for providing useful analytical data.
Thus, an assay that is selected for large-scale international efficacy trials should exhibit a high throughput format that is suitable to analyze thousands of cryopreserved specimens as well as exhibit high levels of sensitivity, specificity and reproducibility.
Moreover, it is important to state that optimizing an assay does not equate with validating the assay, nor does repeating the assay a number of times constitute validation.
www.niaid.nih.gov /hivvaccines/validation.htm   (821 words)

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