Factbites
 Where results make sense
About us   |   Why use us?   |   Reviews   |   PR   |   Contact us  

Topic: Autoradiography


Related Topics

In the News (Mon 6 Jul 09)

  
  Autoradiography and fluorography
Autoradiography is the direct exposure of film by beta particles or gamma rays.
Fluorography is the exposure of film by secondary light that was generated by the excitation of a fluor or a screen by a beta particle or a gamma ray.
Note that the image provided by direct autoradiography is always sharper than is the image resulting from fluorography.
pingu.salk.edu /~sefton/Hyper_protocols/ARG.html   (478 words)

  
 Carestream Health: Autoradiography Films   (Site not responding. Last check: 2007-10-26)
Carestream Health is the worldwide leader in developing and manufacturing autoradiography films for life science research.
First there was KODAK X-Omat AR film, a general purpose, high performance autoradiography film, that soon became the world's research imaging standard.
Today Molecular Imaging Systems continues this tradition of high performance KODAK autoradiography films with the BioMax family of films, the new standard for life science imaging.
www.kodak.com /US/en/health/s2/products/autoradFilms/index.jhtml   (273 words)

  
  Autoradiography by Jeff Walding
Autoradiography is a technique that is used to study biochemical processes by labeling sites that contain radioisotopes.
However, this could prove to be a limitation of autoradiography because often times the size of the silver grain is larger than the organelle, which makes it impossible to identify the exact site of origin (Karp, 1996).
The technique of autoradiography has proven to be a valuable resource when determining the site of synthesis and the movement of molecules in various biochemical processes.
www.samford.edu /~gekeller/walding.html   (784 words)

  
 ILFORD Nuclear Emulsions FAQs   (Site not responding. Last check: 2007-10-26)
Biological autoradiography takes advantage of the fact that animals and plants cannot distinguish between stable and radioactive isotopes of the same elements in their physiological reactions.
Autoradiography has provided a useful tool in metallurgy, such as in the investigation of the distribution of different metals within an alloy.
Autoradiography may also be used in studies of frictional damage by machinery.
www.ilford.com /html/us_english/prod_html/nuclear/Autoradiography.html   (4247 words)

  
 Principles of autoradiography
Autoradiography is a procedure for localizing and recording a radiolabeled compound within a solid sample, which involves the production of an image in a photographic emulsion.
Direct autoradiography involves placing the sample in intimate contact with an X-ray film, a plastic sheet with a coating of photographic emulsion; the radioactive emissions from the sample produce dark areas on the developed film.
Indirect autoradiography is a modification in which the emitted energy is converted to light by a suitable chemical (scintillator or fluor).
www.ncbi.nlm.nih.gov /books/bv.fcgi?rid=hmg.box.472   (344 words)

  
 JAIC 1983, Volume 22, Number 2, Article 8 (pp. 101 to 102)
The book Art and Autoradiography: Insights into the Genesis of Paintings of Rembrandt, Van Dyck and Vermeer should be viewed as an example of the technique applied to well known paintings, with the art historian, conservator, and museum scientist working together.
They clearly indicate that “autoradiography by itself cannot be touted as the final word” and describe its use with other complementary methods such as x-ray radiography and analysis of pigment sample cross sections.
X-ray radiographs show some details lost to autoradiography and the reverse is also true; the authors clearly illustrate that they should be used together whenever possble.
aic.stanford.edu /jaic/articles/jaic22-02-008_indx.html   (774 words)

  
 FREQUENTLY ASKED QUESTIONS ABOUT AUTORADIOGRAPHY   (Site not responding. Last check: 2007-10-26)
Autoradiography is a technique that enables scientists to locate the distribution of radioactive compounds in a sample.
Autoradiography has provided a useful tool in metallurgy and the study of chemically reactive surfaces.
For a first attempt at Autoradiography for light microscopy we recommend the use of ILFORD K5 because of this higher sensitivity.
www.ilford.com /html/us_english/prod_html/nuclear/faq1.html   (1545 words)

  
 [No title]   (Site not responding. Last check: 2007-10-26)
For $^3$H autoradiography, the efficiency is low, to achieve the highest resolution one must cut thin sections, and high doses and long exposure times are required.
For $^{14}C$ autoradiography, typical slice thicknesses are 20-30 $\mu$m, which is roughly the achievable rms resolution.
Behaviors in "freely moving" subjects A problem for both human and animal studies, using autoradiography or non-invasive imaging methods, is the intrusive effect of the system for tracer delivery and scanning.
www.ps.uci.edu /~markm/dan/london/london_072204.txt   (753 words)

  
 Autoradiography   (Site not responding. Last check: 2007-10-26)
L-Plus screens are especially suitable for autoradiographic applications where reduced exposure times are required with the use of high-energy beta and gamma particle emitters such as 32P, 175I and 45CA.
High Plus screens are also recommended for reduced autoradiography exposure times.
Relative to L-Plus, High Plus screens provide higher resolutions with slightly longer exposure times.
www.mcio.com /autoradi.htm   (279 words)

  
 History of Autoradiography   (Site not responding. Last check: 2007-10-26)
Autoradiography has a wide range of spatial resolutions; microautoradiography, with resolution down to 0.05 µm, is used to locate tracers within or between cells; macroautoradiography, with resolution of about 50 µm, is used to determine tracer concentration within tissues.
The first biological experiment involving autoradiography traces the distribution of polonium in biological specimens.
The use of autoradiography is also proposed for the measurement of ligand binding and drug interaction.
laxmi.nuc.ucla.edu:8248 /M248_03/autorad/intro_hist.html   (262 words)

  
 Autoradiography   (Site not responding. Last check: 2007-10-26)
Autoradiography is a photochemical detection method in which a radioactive sample is placed above a photographic emulsion or film.
Autoradiography, may be combined with the study of cell physiology by feeding cells specific compounds with radioactive atoms and then microscopically observing on a photographic emulsion the distribution of radioactive events.
The technique of labeling compounds with radioactive isotopes makes it possible to study the distribution and concentration of isotopes in tissue slices similar to those studied routinely under the microscope.
home.earthlink.net /~dayvdanls/GET1.htm   (135 words)

  
 Direct Beta Autoradiography using MCP Detectors
At present most digital autoradiography methods have great difficulty in efficiently detecting tritium due to the low energy of the emitted beta.
This line of research, new within the Physics Department, forms the basis of an inter-disciplinary research project involving other departments (Genetics, CMHT and Dermatology) within our own University and extending existing collaborations with other laboratories (such as the Institute of Cancer Research in Sutton) and commercial companies (Photonis SAS and Photek Limited).
C Autoradiography, Nuclear Instruments and Methods in Physics Research A, 45 (1998).
www.star.le.ac.uk /mcp/betaauto   (374 words)

  
 Autoradiography
Autoradiography with GaAs pixel detectors : genetics and dynamic studies (Medipix electronics)
You can see here a 5x5 mm2 autoradiography of mammary cell clusters, obtained in a 10 minute exposure time, using 32P-orthophosphate in acqueous solution as a marker: we estimated about 77 nCi total activity of the clusters.
Our present set-up is based on a GaAs pixel array (64x64 pixels, 170 micron size) and bump-bonded low-noise electronics (Medipix), developed within an international collaboration for Digital Radiography.
www.na.infn.it /mfa/med/autoradiography.html   (772 words)

  
 Neurological Research: Protective effect of FK506 in the reperfusion model after short-term occlusion of middle ...   (Site not responding. Last check: 2007-10-26)
The protective effect of the immunosuppressant agent FK506 in the reperfusion after short-term occlusion of the middle cerebral artery in the rat model was evaluated using [125l]PK-11195 autoradiography.
This study investigated the protective effect of FK506 during short-term occlusion of the MLA with reperfusion in the rat assessed by autoradiography using [1251]PK11195.
Our autoradiography results using [1251]PK11195 clearly delineated the infarcted area and allowed measurement of the density of ['ZSl]PK-11195.
www.findarticles.com /p/articles/mi_qa3909/is_200009/ai_n8909853   (1243 words)

  
 Spatial mapping of phosphorus influx in bean root systems using digital autoradiography -- Rubio et al. 55 (406): 2269 ...
The grey intensity is proportional to the phosphorus accumulation.
Relationship between the root length measured by analysing the digital autoradiography images of the ionizing radiation emitted by the roots (as in Fig.
Owusu-Bennoah E, Wild A. Autoradiography of the depletion zone of phosphate around onion roots in the presence of vesicular-arbuscular mycorrhiza Glomus mosseae.
jxb.oxfordjournals.org /cgi/content/full/erh246?ijkey=28Z3Bk1uKxCVc&keytype=ref   (5991 words)

  
 Principles_of_autoradiography   (Site not responding. Last check: 2007-10-26)
Autoradiography is a procedure for localizing and recording a radiolabled compound within a solid sample, which involves the production of an image in a photographic emulsion.
Indirect autoradiography is a modification in which the emitted energy is converted to light by a suitable chemical (scintillator or fluor) using either of the following methods:
By effectively superimposing a photographic emission upon the direct autoradiographic emission, the image is intensified.
www.fmv.ulg.ac.be /genmol/MODGEN/ChapterIII/Principles_of_autoradiography.htm   (406 words)

  
 GE Healthcare - formerly Amersham Biosciences - Products - Autoradiography Films, Screens and Accessories
Ideal for whole body autoradiography, in situ hybridization, and receptor localization applications.
Emulsion coating on both sides provides high sensitivity for both direct exposure autoradiography and exposures with Hyperscreen™, BioMax™ MS or TranScreen Intensifying Screens.
Robust and chip-proof autoradiography cassettes for light-tight exposures at temperatures as low as -70 °C. Maximum contact between film and sample.
www5.amershambiosciences.com /APTRIX/upp01077.nsf/Content/Products?OpenDocument&parentid=46987   (743 words)

  
 "Inquiry-more you can do with Inquiry"   (Site not responding. Last check: 2007-10-26)
The identification, localization, and measurement of receptors using receptor autoradiography is important in studies of the mechanism of action and efficacy of drugs, neurotransmitters, neuropeptides, and hormones.
Glucose Metabolism Cerebral Blood Flow Metabolic Autoradiography techniques facilitate the measurement of regional biochemical and physiological characteristics of brain metabolic function in experimental conditions of altered physiological states as well as pathological conditions.
Karyotyping, in conjunction with chromosome staining and banding techniques, is useful for classicification of genetic disease, evaluation of mutagenicity and in fundamental genetic research.
www.loats.com /inq_more.html   (221 words)

  
 Bioimaging at Leicester University - Publications
Autoradiography of high energy radionuclides using MCP detectors, IEEE Trans.Nuc.Sci,(2001)
Direct Beta Autoradiography using MCP Detectors, Nuclear Instruments and Methods in Physics Research A, 392,349-353, (1997).
See also the Microsoft Powerpoint presentation Efficiency Enhancements for MCP based Beta Autoradiography.
www.src.le.ac.uk /projects/bioimaging/publications   (132 words)

  
 Neuroscience-Net-Pharmacology Section   (Site not responding. Last check: 2007-10-26)
This study focused secondarily on mapping the neuroanatomical distribution of D1 receptors in squirrel monkey brain with quantitative receptor autoradiography, which, to our knowledge, has yet to be described in the literature.
Tissue sections containing caudate nucleus and putamen were used in parametric studies to establish appropriate conditions for dopamine D1 and D2 receptor autoradiography.
H]WIN 35,428 autoradiography was conducted using a modification of the method of Canfield et al.
www.neuroscience.com /mjk0696/htm/main.html   (7584 words)

  
 Bioresearch Online: Digital Marketplace for the science industry
Kodak X-Omat AR film is the world standard for a general-purpose autoradiography film.
Kodak continues this tradition of high performance autoradiography films with the BioMax family of films, the NEW standard for life science imaging.
X-OMAT AR Film is an autoradiography film for use with all commonly used isotopes and chemiluminescence labels.
www.bioresearchonline.com /Content/ProductShowcase/product.asp?DocID={967547CE-DA83-11D3-8C1C-009027DE0829}   (202 words)

  
 Autoradiography Wax
When used with detection methods which rely on low energy radiation detection of light emissions, EA-Wax can dramatically enhance detection efficiencies and significantly reduce the time required to obtain experimental results.
The Enhanced Autoradiography process is a low-cost, environmentally friendly and inherently safe process.
Enhanced Autoradiography using EA-Wax offers many benefits to researchers in University life science departments, hospitals, pharmaceutical companies and research institutes.
www.gentaur.com /ea_wax.htm   (109 words)

  
 tlc_txt   (Site not responding. Last check: 2007-10-26)
Standard autoradiography was used in an attempt to visualise the spatial distribution of the various tritiated(
The same TLC plate was then prepared for Enhanced Autoradiography.
The superior detection efficiency of Enhanced Autoradiography over autoradiography is obvious, by visual inspection.
www.eabiotech.com /tlc_txt.htm   (213 words)

  
 Amazon.ca: Books: Receptor Autoradiography: Principles & Practice   (Site not responding. Last check: 2007-10-26)
Receptor autoradiography is an invaluable technique that is widely used to localize and characterize peptide and neurotransmitter binding sites.
This instructive work makes receptor autoradiography more accessible by giving a detailed account of the methodology and its application in the analysis of receptors in the brain and peripheral organs.
These examples are not exhaustive but illustrate the versatility of the technique as well as its potential pitfalls.
www.amazon.ca /exec/obidos/ASIN/0192622099   (363 words)

  
 autoradiography - OneLook Dictionary Search
Tip: Click on the first link on a line below to go directly to a page where "autoradiography" is defined.
Autoradiography : Drug Discovery and Development [home, info]
Autoradiography : Ethics, Law and Science of Using New Genetic Technology in Medicine and Agriculture [home, info]
www.onelook.com /?w=autoradiography   (143 words)

  
 Autoradiography definition - Medical Dictionary definitions of popular medical terms
Autoradiography definition - Medical Dictionary definitions of popular medical terms
Autoradiography: A technique using X- ray film to visualize molecules or fragments of molecules that have been radioactively labeled.
Autoradiography can, for example, be used to analyze the length and number of DNA fragments after they are separated from one another by a method called gel electrophoresis.
www.medterms.com /script/main/art.asp?articlekey=2405   (104 words)

  
 Radiolabeled Native Low-Density Lipoprotein Injected Into Patients With Carotid Stenosis Accumulates in Macrophages of ...
For autoradiography, sections were coated with Ilford K2 emulsion
LDL was detected in atherosclerotic plaques by light microscopy autoradiography.
Autoradiography and immunohistochemical autoradiography of atherosclerotic plaques of patients injected with radiolabeled LDL or radiolabeled human serum albumin.
circ.ahajournals.org /cgi/content/full/101/11/1249   (3086 words)

  
 Photography
Optimized for use with BioMax MS film, but can be used with other films.
For use with all Kodak autoradiography films with a standard 15W or smaller frosted bulb; with X-OMAT AR, use a 7.5W bulb.
Bright green ink is visible in normal light, and phosphoresces with an emission peak at 560 nm.
www.sigmaaldrich.com /Area_of_Interest/Equipment_Supplies__Books/Photography.html   (1381 words)

  
 General Laboratory Techniques/Autoradiography Protocols
Added: Tue May 14 2002, Hits: 1735, Reviews: 0 Write review Cached
Autoradiography of Tissue Sections (Gene Delivery Group, University of Birmingham)
Added: Mon Sep 09 2002, Hits: 1948, Reviews: 0 Write review Cached
www.protocol-online.org /prot/General_Laboratory_Techniques/Autoradiography   (93 words)

  
 Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies -- Perry et al. 289 ...
Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies -- Perry et al.
Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies
Autoradiography was carried out according to published methods (Perry and Kellar, 1995
jpet.aspetjournals.org /cgi/content/full/289/3/1545   (5358 words)

Try your search on: Qwika (all wikis)

Factbites
  About us   |   Why use us?   |   Reviews   |   Press   |   Contact us  
Copyright © 2005-2007 www.factbites.com Usage implies agreement with terms.