For the Bacilalles data set, the branches of the DNA topology were found from 261 to 662 topologies, with bootstrap average value of 82,7% (± 17.3).
The branch presented exclusively in the concatenated protein topology was found in 153 partition topologies, with bootstrap average value of 60.4% (± 19.6), whereas the branch presented only in the concatenated DNA topology was found in 261 partitions with bootstrap average value of 67.6% (± 20.6).
The next steps of this work is to include these new OTUs in order to test the influence of the long branches, and to construct the partition trees using the protein sequences and other methods in order to search for the differences between DNA and protein sequences as well as among the methods.
The large number of proteins grouped into GntR and GalR/LacI families may provide these bacteria the ability to grow in the presence of several carbon sources and to rapidly adapt their gene expression to changing nutrient conditions as has been suggested previously [26].
It should be noted that most Bacillales include a phase of sporulation in their life cycle.
Further research is necessary to determine the physiological function of species-specific and shared transcriptional regulators that might be involved either in the regulation of cellular processes relevant for biotechnological production or that might control the expression of genes involved, for instance, in virulence of pathogenic bacteria.
Bacillus (Geobacillus) stearothermophilus Strain 10 is being sequenced by Sharon Lewis, a graduate student in our laboratory at the University of Oklahoma, Norman Campus's Advanced Center for Genome Technology, in the Department of Chemistry and Biochemistry, which is funded in part by an NSF EPSCoR grant
To date, we now are sequencing individual, shotgun-based, double stranded templates generated from a randomly sheared library of the Bacillus (Geobacillus) stearothermophilus genome, with both the universal forward and reverse pUC sequencing primers.
www.genome.ou.edu /bstearo.html (813 words)
morphfig2(Site not responding. Last check: 2007-11-05)
Comprehensive RDP tree based on the 16S rRNA gene sequences of members of the Order Bacillales mapped with classical morphologies.
The Order Bacillales is one branch of the Low G+C lineage from Figure 1.
Blue designates cocci morphology and green indicates rod morphology.
by clones related to Clostridiales, Lactobacillales, and Bacillales
Assignable phylogenetic groups are given in parentheses with the following abbreviations: AB, Actinobacteria; B, Bacillales; CFB, CFB phylum; C, Clostridiales, LB, Lactobacillales; P, Planctomycetales; ßPB, ß-Proteobacteria; n.a., not assignable.
Boldface T-RFs in the soil profiles indicate major T-RFs without a corresponding major T-RF in the gut profiles.
