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Topic: Bdellovibrio


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  Bdellovibrio - Wikipedia, the free encyclopedia
Bdellovibrio is a genus of Gram-negative, obligate aerobic bacteria.
Under the microscope, a Bdellovibrio appears as a comma-shaped motile rod that is about 0.3-0.5 by 0.5-1.4 µm in size with a barely discernible flagellum.
Bdellovibrio attacks other gram-negative bacteria by colliding with them at a relatively blazing speed of 100 µm per second, which is about 100 times its size.
en.wikipedia.org /wiki/Bdellovibrio   (352 words)

  
 Bdellovibrio -- Facts, Info, and Encyclopedia article   (Site not responding. Last check: 2007-09-15)
To enrich for Bdellovibrio use NB/500 (nutrient broth at 1:500 dilution) and mix with hot soft (Any culture medium that uses agar as the gelling agent) agar with (A species of bacterium normally present in intestinal tract of humans and other animals; sometimes pathogenic; can be a threat to food safety) E.
Under the microscope, a Bdellovibrio appears as a comma-shaped motile rod that is about 0.3-0.5 by 0.5-1.4 (Click link for more info and facts about µm) µm in size with a barely discernible (A lash-like appendage used for locomotion (e.g., in sperm cells and some bacteria and protozoa)) flagellum.
Bdellovibrio attacks other gram-negative bacteria by colliding with them at a relatively blazing speed of 100 (Click link for more info and facts about µm) µm per second, which is about 100 times its size.
www.absoluteastronomy.com /encyclopedia/B/Bd/Bdellovibrio.htm   (467 words)

  
 Bdellovibrio
One of its more notable characteristics of this genus is that members parasitize other gram-negative bacteria by entering into their periplasmic space[?] and feeding on the cytoplasmic fluid of it's host.
Under the microscope, a Bdellovibrio appears as a comma-shapped motile rod that is about 0.3 by 1.5µ in size with a barely discernible flagellum.
Bdellovibrio attack other gram-negative bacteria, enter into their periplasmic space, drain the host of nutrients, becomes a spherical bdelloplast, then elongates, and divides.
www.ebroadcast.com.au /lookup/encyclopedia/bd/Bdellovibrio.html   (169 words)

  
 Publications on Bdellovibrio Bacteriovorus   (Site not responding. Last check: 2007-09-15)
Intraperiplasmic growth of Bdellovibrio bacteriovorus 109J: solubilization of Escherichia coli peptidoglycan.
Intraperiplasmic growth of Bdellovibrio bacteriovorus 109J: attachment of long-chain fatty acids to Escherichia coli peptidoglycan.
A conjugation procedure for Bdellovibrio bacteriovorus and its use to identify DNA sequences that enhance the plaque forming ability of a spontaneous host-independent mutant.
www.msu.edu /~mftlab/bdell.html   (173 words)

  
 A killer microbe as a living antibiotic
Bdellovibrio bacteriovorus is a fascinating predatory bacterium that attaches specifically to certain other bacteria in order to invade them.
Bdellovibrio can remain encysted at this stage, while the entry pore has been sealed and the prey cell remains viable.
These attributes, together with the facts that certain Bdellovibrio strains show a very narrow prey spectrum and are capable of penetrating the same tissues as may human-pathogens, gives promise to the development of novel anti-microbial strategies.
www.eurekalert.org /pub_releases/2004-01/m-akm012704.php   (687 words)

  
 Bdellovibrio
Bdellovibrio is placed with Myxoccus in the delta subdivision of the phototrophic purple-bacteria.
After the Bdellovibrio is attached to the cell wall it undergoes an irreversible stage in which it penetrates the preys cell wall.
One of the most unusual characteristics of the Bdellovibrio is its ability to modify the structure and function of the host cells organelles and structure, such as the cell wall.
web.umr.edu /~microbio/BIO221_1999/Bdellovibrio.html   (904 words)

  
 Special Instructions: Cultivation of Bdellovibrios
Bdellovibrios can be found in a wide variety of habitats, ranging from soil to sewage, provided these environments are densely populated with bacteria.
For the microscopical observation of attack-phase bdellovibrios with their characteristic swift motility remove a sample from the cleared zone of the top agar that is adjacent to the boundary of intact prey cells (area is marked by a red box in Fig.
This suspension is inoculated with 100 µl of a fresh bdellovibrio lysate and incubated for 24 to 48 h to lyse all the prey cells.
www.dsmz.de /media/hint2.htm   (1033 words)

  
 Bacteriophage Ecology Group - Reference Abstracts (1976)
Failure of bdellovibrio parasitization was unrelated to gonococcal species, colony type, piliation, penicillin susceptibility, or virulence as influenced by passage in embryonated eggs.
Bdellovibrio was able to multiply on the high numbers of bacteria present in the aerobic percolating filter film but could not survive in anaerobic sludge.
Bdellovibrio proved to play an insignificant role in the processes of self-purification from the intestinal microbial flora of weakly and moderately contaminated sea water.
www.mansfield.ohio-state.edu /~sabedon/ab1976.htm   (5656 words)

  
 Untitled   (Site not responding. Last check: 2007-09-15)
Bdellovibrio are gram negative obligate parasitic bacteria, which require certain living conditions to grow.
The Bdellovibrio and E.coli are mixed into a soft top layer and then poured onto a solid bottom layer to provide a fluent mixture.
Presence of Bdellovibrio is determined by examining the plates two to three days later for plaques, clear spots of lysed out bacteria that appear after 48 hours of incubation.
www.wam.umd.edu /~changh/Introduction.htm   (163 words)

  
 Bacteriophage Ecology Group - Reference Abstracts (1977)
The maximum rate of infection was found at 37 degrees C. The maximum yield of Bdellovibrio and the maximum lysis of the host cells occurred at 22.5 degrees C. The cardinal points, at which no interaction was observed, have been determined.
Bdellovibrio underwent photooxidation by visible light in the presence of exogenous photosensitizer and by near-ultraviolet light (325 to 400 nm) in its absence.
Bdellovibrio inoculated into rabbit ileal loops was greatly reduced in number within 24 h.
www.mansfield.ohio-state.edu /~sabedon/ab1977.htm   (6141 words)

  
 Prey Range Characterization, Ribotyping, and Diversity of Soil and Rhizosphere Bdellovibrio spp. Isolated on ...
Thirty new Bdellovibrio strains were isolated from an agricultural soil and from the rhizosphere of plants grown in that soil.
The Bdellovibrio and prey strains used are shown in Tables 1 and 2, respectively; the origins of the strains are also shown.
bdellovibrios are components of biofilms (13, 31) and exhibit
aem.asm.org /cgi/content/full/66/6/2365   (4749 words)

  
 Genomics and Signal Transduction Research Group - Stephan C. Schuster - MPI Tübingen   (Site not responding. Last check: 2007-09-15)
The life cycle of Bdellovibrio based on previous data is being extended accordingly to the predicted physiology.
In its life cycle Bdellovibrio grows and develops in the periplasm of its prey.
Understanding the modes of operation and the absence of type III and type IV secretion systems encourages for the development of Bdellovibrio as basis for antibiotics as in addition no reports of invading mammalian cells are known.
www.eb.tuebingen.mpg.de /schuster/research_bd.htm   (348 words)

  
 Press Releases - Public Affairs Office - The University of Nottingham   (Site not responding. Last check: 2007-09-15)
Bdellovibrio, bacteria that were discovered in 1962, are about six times smaller than the E.coli bacteria.
As Bdellovibrio only grow and divide by eating other bacteria and are not harmful to human or animal cells, the bacteria could be used in the future as ‘living antibiotics’ to treat persistent bacterial infections.
The University laboratory has specific expertise in the biology of Bdellovibrio bacteria and in altering their genes and testing the effects of these alterations on how efficiently the Bdellovibrio prey on other bacteria.
www.nottingham.ac.uk /public-affairs/press-releases/index.phtml?menu=pressreleases&code=PRED-15/04&create_date=05-feb-2004   (395 words)

  
 Bdellovibrio   (Site not responding. Last check: 2007-09-15)
Bdellovibrio es un género de gram-negative, obliga bacterias aerobias.
Debajo del microscopio, un Bdellovibrio aparece como barra motile coma-formada que sea cerca de 0.3-0.5 al lado de 0.5-1.4 µm de tamaño con un flagelo pelado perceptible.
Bdellovibrio ataca otras bacterias gram-negative chocando con ellas a una velocidad relativamente ardiente del µm 100 por el segundo, que es cerca de 100 veces su tamaño.
www.yotor.net /wiki/es/bd/Bdellovibrio.htm   (308 words)

  
 Discussion   (Site not responding. Last check: 2007-09-15)
Confirmation of the isolation was made by observations of the plates on which the organism grew, the isolated organisms’ morphological characteristics, a gram stain test and an oxidase test.
Bdellovibrio can be found in its natural habitat in soil, sewage, freshwater, and marine environments.
Today Bdellovibrio are being used in studies of food spoilage; the bdellovibrio could potentially lyse gram-negative pathogens and spoilage bacteria in the food we eat.
www.wam.umd.edu /~changh/Discussion.htm   (285 words)

  
 Natural predators - Edition 5 - Nottingham University | Research | Vision Magazine
Bdellovibrio are one of few bacterial species known to prey on other bacteria, and can be found not only in the human body, but also in water and soil; anywhere in fact where there are other bacteria.
Once they are inside, Bdellovibrio settle between the two membranes and set about eating the prey from the inside, breaking them down into their basic components.
The Bdellovibrio lifecycle was described in 1962, but the genetics of the process has so far remained a mystery.
research.nottingham.ac.uk /Vision/display.aspx?id=991&pid=204   (495 words)

  
 Biophysical Journal: Investigations into the life cycle of the bacterial predator Bdellovibrio bacteriovorus 109J at an ...
On the filters, wild-type bdellovibrios might have "grazed" on the remaining debris of lysed cells, permitting them to stay long and healthy even after the local prey cells were all killed.
The presence of this coating was not observed by AFM with wild-type bdellovibrios.
Truly host-independent bdellovibrios are thought to arise from at least two mutations, whereas host-independent bdellovibrios arising from a single mutation grow only in the presence of other host-independent bdellovibrios or cell extracts (Thomashow and Cotter, 1992).
www.findarticles.com /p/articles/mi_qa3938/is_200305/ai_n9273900/pg_4   (1399 words)

  
 Eaten alive: Bacteria offers a new way to treat persistent infections
Bdellovibrio (literally, 'curved leech') is one of the bacteria being studied by Dr Liz Sockett in the Genetics Department at the University of Nottingham.
As well as working on typical laboratory bugs, while on a visit to Oxford she was introduced to Bdellovibrio - the 'Thorpedo' of the bacterial world, able to propel itself 70-100 body lengths every second (imagine a human running at 400 mph).
Bdellovibrio attach to their target, burrow through the outer membrane and take up residence in the periplasmic space.
www.wellcome.ac.uk /doc_WTD004687.html   (880 words)

  
 Fate of Predator and Prey Proteins during Growth of Bdellovibrio bacteriovorus on Escherichia coli and Pseudomonas ...
Two-dimensional gel electrophoresis of proteins from (a) a washed, fresh bdellovibrio culture of a two membered culture of Escherichia coli and Bdellovibrio bacteriovorus 109J-1, (b) Escherichia coli, (c) a washed, fresh bdellovibrio culture of a two-membered culture of Pseudomonas syringae pv.
Bdellovibrio bacteriovorus strains produce major outer membrane protein during predacious growth in the periplasm of prey bacteria.
Uptake of intake nucleoside monophosphates by Bdellovibrio bacteriovorus 109J.
jb.asm.org /cgi/content/full/187/1/329   (3729 words)

  
 Bdellovibrio_bacteriovorus   (Site not responding. Last check: 2007-09-15)
Bdellovibrio bacteriovorus is a highly motile Gram-negative delta-proteobacterium that preys on other bacteria.
Bdellovibrio are ubiquitous in nature, having been discovered in a wide variety of environments that include both aquatic and terrestrial habitats as well as mammalian intestines.
Studying the molecular mechanisms of Bdellovibrio's degradative enzymes gives insight into those targets in prey cells that have proven to be evolutionarily successful points of attack and offers the possibility for the design of antimicrobial agents.
www.ebi.ac.uk /2can/genomes/bacteria/Bdellovibrio_bacteriovorus   (283 words)

  
 Bdellovibrio
One of its more notable characteristics of this genus is that members parasitize other gram-negative bacteria by entering into their periplasmic space and feeding on the biopolymers, e.g.
In most cases however Bdellovibrio moves on and devours its prey.
At the completion of its life cycle, which takes from one to three hours, the Bdellovibrios burst free from the remainder of the host cell in order to attack new preys.
www.sciencedaily.com /encyclopedia/bdellovibrio   (344 words)

  
 The Curious Microbe: Bdellovibrio
The Bdellovibrio (which literally means "curved leech") make a living by attacking and devouring other bacteria, and are found in diverse environments such as marine and fresh waters, sewage, and soil.
The Bdellovibrio growth phase is the period when new cells are created and requires the parasitism of a suitable host cell.
The growing Bdellovibrio is now considered a parasite and continues to elongate into a filament.
commtechlab.msu.edu /sites/dlc-me/curious/caOc96LC.html   (612 words)

  
 Health: Bad Bug Does Good - Newsweek: International Editions - MSNBC.com
The potential of Bdellovibrio as a weapon against bacterial infections may seem obvious, but until biochemist Stephan Schuster of the Max-Planck Institute in Tubingen, Germany, thought to look in 2002 nobody had studied the complete genome of this benign bug.
And Bdellovibrio is itself a product of evolution—so it will probably continue to make the adaptations needed to keep a leg up on its prey.
Because Bdellovibrio attacks cells that are no longer growing, it might be well suited to treating late-stage infections.
msnbc.msn.com /id/4123505   (721 words)

  
 Metabolism of RNA-ribose by Bdellovibrio bacteriovorus during intraperiplasmic growth on Escherichia coli.
Metabolism of RNA-ribose by Bdellovibrio bacteriovorus during intraperiplasmic growth on Escherichia coli.
Metabolism of RNA-ribose by Bdellovibrio bacteriovorus during intraperiplasmic growth on Escherichia coli.No similar effects were found with added ribose-5-phosphate.
During intraperiplasmic growth of Bdellovibrio bacteriovorus 109J on Escherichia coli some 30 to 60% of the initial E. coli RNA-ribose disappeared as cell-associated orcinol-positive material.
www.pdg.cnb.uam.es /UniPub/iHOP/gp/3165311.html   (376 words)

  
 Investigations into the Life Cycle of the Bacterial Predator Bdellovibrio bacteriovorus 109J at an Interface by Atomic ...
A Bdellovibrio predator and the killed prey cell in which it is growing are together termed a bdelloplast.
Arrows indicate bdellovibrios that were in the process of attacking prey cells.
The prey bacteria, which have a life cycle with a much shorter period, multiplied at the edges of the circular community away from the predator bacteria at the center, and thus a gradient of predation stages was present on the filter.
www.biophysj.org /cgi/content/full/84/5/3379   (5638 words)

  
 Effects of methotrexate on intraperiplasmic and axenic growth of Bdellovibrio bacteriovorus.
Total bdellovibrio DNA after growth on the depleted E. coli in the presence or absence of methotrexate exceeded the initial quanity of E. coli DNA present.
Mexthotrexate (10-3 M) inhibited intraperiplasmic growth of bdellovibrio on the depleted E. coli somewhat more than it inhibited growth on normal E. coli, but the effects were small compared with inhibition of axenic growth of the mutant.
The intraperiplasmic growth rate and cell yield of wild-type Bdellovibrio bacteriovorus 109J, growing on Escherichia coli of normal composition as the substrate, were not markedly inhibited by 10-3 M methotrexate (4-amino-N10-methylpteroylglutamic acid).
www.pdg.cnb.uam.es /UniPub/iHOP/gp/2250567.html   (297 words)

  
 Bdellovibrio bacteriovorus Strains Produce a Novel Major Outer Membrane Protein during Predacious Growth in the ...
Bdellovibrio bacteriovorus Strains Produce a Novel Major Outer Membrane Protein during Predacious Growth in the Periplasm of Prey Bacteria -- Beck et al.
Bdellovibrio bacteriovorus is a predatory bacterium that is
Bdellovibrio possesses a prey-derived OmpF protein in its membrane.
jb.asm.org /cgi/content/full/186/9/2766   (4423 words)

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