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Topic: Beta-galactosidase

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Beta Galactosidase Beta galactosidase the molecule rotating on the left is an enzyme that initiates the breakdown of the sugar lactose. Beta galactosidase also forms a complex with ONPG molecules and is able to hydrolyze them. This bridge can be broken when the enzyme beta galactosidase binds to lactose and a water molecule reacts with the oxygen atom in the bridge. biology.kenyon.edu /courses/biol09/betagal/FRAMES/captxt.htm   (515 words)

beta Galactosidase antibody (ab616) datasheet Beta galactosidase is a a metalloenzyme that that splits lactose into glucose and galactose and is coded by a gene (lac z) in the lac operon of Escherichia coli. If beta Galactosidase antibody (ab616) does not perform as described on this datasheet, notify us within 90 days of delivery using this form, so that we can help you or offer you a replacement or a refund. At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta Galactosidase antibody is on this datasheet. www.abcam.com /?datasheet=616   (696 words)

beta-Galactosidase Antibody Staining Protocol Description: Beta galactosidase is a a metalloenzyme that that splits lactose into glucose and galactose and is coded by a gene (lac z) in the lac operon of Escherichia coli. www.ihcworld.com /_protocols/antibody_protocols/b-gal_abcam.htm   (78 words)

Beta Galactosidase antibodies from RDI Divison of Fitzgerald Industries Intl Reactivity: MoAb 2E9 reacts with beta galactosidase of E. Coli and Aspergillus oryzae. This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a balb/c mice immunized with purified beta galactosidase of E. Coli. Cross reactivity with beta galactosidase from bovine liver less than 10%. www.researchd.com /miscabs/bgalacto.htm   (344 words)

lacZ - Germ line transformation experiments with the upstream region of the D.melanogaster beta 2 tubulin gene fused to the Escherichia coli lacZ gene resulted in the correct tissue specific expression of the reporter gene. The lethal E phi lacZ fusions are also lethal to a mutant, designated slyD, which was isolated as a spontaneous E. coli mutant resistant to the expression of the intact E gene. The strains of E. coli that we have constructed are temperature-sensitive for the Mcr and Mrr systems and have been further modified to include a lacZ gene fused to the damage-inducible dinD locus of E. coli. www.ihop-net.org /UniPub/iHOP/gg/168125.html   (8019 words)

Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli. -- Hancock et al. 173 (10): 3084 -- The Journal of Bacteriology Roth, N. J., Huber, R. The beta -Galactosidase (Escherichia coli) Reaction Is Partly Facilitated by Interactions of His-540 with the C6 Hydroxyl of Galactose. Strey, J., Wittchen, K. D., Meinhardt, F. Regulation of beta -Galactosidase Expression in Bacillus megaterium DSM319 by a XylS/AraC-Type Transcriptional Activator. Zhang, J.-H., Dawes, G., Stemmer, W. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. jb.asm.org /cgi/lookup?vol=173&fp=3084&view=abstract   (352 words)

Lactase enzyme supplement (Beta-galactosidase) Deficiency of intestinal beta galactosidase activity causing intolerance to milk-containing foods which presents clinically as abdominal cramps, diarrhoea and bloating. Diagnosis today is made by a positive lactose H2 breath test but if contrast studies are done, the addition of lactose to the barium produces barium dilution, bowel dilatation and rapid transit. Generally, however, lactase usage has not reached its full potential, as present enzymes are relatively expensive and can only be used at low temperatures. www.greatvistachemicals.com /biochemicals/lactase.html   (826 words)

Senescence-associated beta-galactosidase histochemistry for the primate eye -- Mishima et al. 40 (7): 1590 -- Investigative Ophthalmology & Visual Science beta- galactosidase histochemistry assay, adapted for use in the primate Uniform staining of the RPE for pH 4 beta- galactosidase was seen in both Senescence associated {beta} galactosidase activity in human retinal pigment epithelial cells exposed to mild hyperoxia in vitro www.iovs.org /cgi/content/abstract/40/7/1590   (538 words)

Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase. -- Trimbur et al. 60 (12): 4544 -- Applied and Environmental Microbiology Coombs, J. M., Brenchley, J. Biochemical and Phylogenetic Analyses of a Cold-Active beta -Galactosidase from the Lactic Acid Bacterium Carnobacterium piscicola BA. Sheridan, P. P., Brenchley, J. Characterization of a Salt-Tolerant Family 42 beta -Galactosidase from a Psychrophilic Antarctic Planococcus Isolate. aem.asm.org /cgi/lookup?vol=60&fp=4544&view=abstract   (433 words)

MedlinePlus Medical Encyclopedia: Morquio syndrome Type B disease results from deficiency of the enzyme beta galactosidase. Two forms are recognized, type A and type B. Type A disease is characterized by the absence of the enzyme galactosamine-6-sulfatase, and the excretion of keratan sulfate in the urine. Morquio syndrome is an inherited disease belonging to the group of mucopolysaccharide storage diseases. www.nlm.nih.gov /medlineplus/ency/article/001206.htm   (572 words)

GTM Specialty Media advertises reagents for beta galactosidase staining.of mouse embryos and tissue. This link is posted for your convenience, we have not used their reagents. A protocol from our research lab will be posted here in the future. www.med.umich.edu /tamc/laczstain.html   (38 words)

beta-galactosidase The other two are shown in "structure" colors where helices are in pink and beta sheets in yellow. The lactose substrate is shown in spacefilling view with CPK colors. Two of the four chains have been colored red and green. www.bio.davidson.edu /courses/genomics/chime/betagal.html   (72 words)

Beta-Galactosidase Staining Kit Compatible With In Vivo and In Vitro Applications—Efficiently identifies beta galactosidase expressing cells in both in vitro and in vivo samples. Ease of Use—Fast staining process allows accurate visualization of in situ beta galactosidase expression for transfection and DNA delivery experiments. Easy and fast identification of beta galactosidase expression for in vitro and in vivo applications www.mirusbio.com /products/transit/galactosidase/index.asp   (136 words)

A Gene Coding for Tomato Fruit beta -Galactosidase II Is Expressed during Fruit Ripening . Cloning, Characterization, and Expression Pattern -- Smith et al. 117 (2): 417 -- PLANT PHYSIOLOGY A Gene Coding for Tomato Fruit beta -Galactosidase II Is Expressed during Fruit Ripening. A Family of at Least Seven beta -Galactosidase Genes Is Expressed during Tomato Fruit Development Down-Regulation of Tomato beta -Galactosidase 4 Results in Decreased Fruit Softening www.plantphysiol.org /cgi/content/abstract/117/2/417   (668 words)

Chemicon - Product #AB1211-5MG - Anti-beta Galactosidase Some cross reactivity with Beta Galactosidase from other species may occur. ELISA: 1:10,000-1:50,000 when assayed against 1 μg of Beta Galactosidase in a standard sandwich ELISA. Immunoglobulin purified by delipidation, salt fractionation and ion-exchange chromatography. www.chemicon.com /Product/ProductDataSheet.asp?ProductItem=AB1211-5MG   (243 words)

Worthington Galactosidase, Beta Dey, P., and Dixon, M.: Separation and Properties of a-Galactosidase and beta-Galactosidase from Cajanus indicus, Biochim Biophys Acta 370, 269, 1974 Pih, N., and Dhurjati, P.: Oscillatory Behavior of beta-Galactosidase Enzyme Activity in Escherichia coli During Perturbed Batch Experiments, Biotechnol Bioeng 29, 292, 1987 Kelemen, M., and Whelan, W.: Inhibition of Glucosidases and Galactosidases by Polyols, Arch Biochem Biophys 117, 423, 1966 www.worthington-biochem.com /BG   (4497 words)

beta-galactosidase The mice carried a marker gene, beta galactosidase, that turns tissues blue. Genetic key to growth of new arteries is identified www.mongabay.com /igapo/biotech/beta-galactosidase.html   (154 words)

Beta Galactosidase Staining Kits Product Catalog > Section 6 > Beta Galactosidase Staining Kits Our Beta-Gal products are for visualizing the expression of the LacZ Reporter Gene in cell culture. Cells expressing active B-galactosidase produce blue colonies when stained with X-Gal. specialtymedia.com /01Products/0601_BetaGalactosidaseStainingKits.htm   (184 words)

beta galactosidase questions Why is ONPG used in the assay for beta galactosidase activity? How many molecules of lactose are associated with each chain of beta galactosidase? How many molecules of ONPG are associated with each chain of beta galactosidase? biology.kenyon.edu /courses/biol09/betagal/question.htm   (132 words)

Beta-Galactosidase Deficiency, Leukocytes Diagnose patients with bets-galactosidase deficiency, Morquio disease type B (MPS IVb), and combined beta- galactosidase/neuraminidase deficiency (galactosialidosis) This test does not rule out a diagnosis of Morquio disease type A (MPS IVa). Quantity not sufficient for analysis; frozen specimen; incorrect specimen collection tube; specimen not received within 48 hours of collection www.labcorp.com /datasets/labcorp/html/chapter/mono/nf00002070.htm   (121 words)

Blau Lab Research Ponte, P., Gunning, P., Blau, H., and Kedes, L. Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution. www.stanford.edu /group/blau/publications.html   (3827 words)

All Beta Galactosidase We have 17 products in: Tags & Cell Markers >> Fusion / Marker Proteins >> Beta Galactosidase www.abcam.com /?c=599   (79 words)

Beta Galactosidase Active Site The Active Site of E597Q Beta Galactosidase Complexed with IPTG web.mit.edu /course/7/7.01x/7.014/structures/bgalcomplexIPTG.html   (10 words)

39B-9 Relationship between beta-galactosidase activity and grape firmness In contrast, Suffolk Red grapes contained higher beta-galactosidase activity (186 units per mg protein), but had a much lower firmness value (less than 1 N). The correlation coefficient (r) between the activity of beta-galactosidase and the firmness of mature grapes were 0.97. The results of this study indicate that there is an inverse relationship between the activity of beta-galactosidase and the firmness of mature grapes. ift.confex.com /ift/2000/techprogram/paper_1764.htm   (10 words)

44C-1 Purification and characterization of grape beta-galactosidase Beta-galactosidase from Einset Seedless grapes was purified 33-fold by affinity chromatography. However, there is no information in the literature regarding the activity of grape beta-galactosidase. The molecular weight of grape beta-galactosidase was 63 kDa. ift.confex.com /ift/2001/techprogram/paper_6171.htm   (10 words)

BTP - Current Students - Emilie Porter Active beta-peptides induce the leakage of beta-galactosidase from peptide-treated B. subtilis cells, as do alpha-helical antibiotic peptides, and this similarity suggests that the beta-peptide mode of action involves disruption of microbial membranes. We have previously identified a 17-residue beta-peptide (called beta-17) with antibiotic activity similar to that of a magainin derivative against a variety of bacteria, including two clinical isolates that are resistant to common antibiotics. We have designed beta-peptides that are helical, cationic and amphiphilic with the intention of mimicking the biological activity of amphiphilic, cationic alpha-helical antimicrobial peptides found in nature (e.g., magainins). www.bact.wisc.edu /Biotech/student/STUDENT/EmiliePorter/profile.htm   (330 words)

Promega Beta-Glo Method for the Veritas multiwell plate microplate luminometer enzyme assays from Turner BioSystems Assay System provides a reliable, homogeneous method to quantitate ß-galactosidase expression in mammalian cells. For 96-well plates, typically 100 µL of reagent is added to cells grown in 100 µL of medium. For optimal results, do not reduce the volume of reagent to less than a 1:1 ratio with the volume of medium. www.turnerbiosystems.com /doc/appnotes/S_0093.html   (813 words)

Sequences Represented in UniVec gi209094gbM21875.1SYNPMTL20P Cloning vector pMTL20P beta-galactosidase alpha-chain and beta- lactamase genes, complete cds gi207990gbM37852.1SYNB2061 Cloning vector pMMB206 beta lactamase (bla, truncated), 3' end gi833821gbU25060.1XXU25060 Plasmid pBBR1MCS-4 plasmid replication REP gene, LacZ alpha peptide (lacZ alpha) gene, and beta lactamase gene, complete cds www.ncbi.nlm.nih.gov /VecScreen/replist.html   (3188 words)

Beta-galactosidase-neuraminidase deficiency: restoration of beta-galactosidase activity by protease inhibitors -- Suzuki et al. 90 (1): 271 -- Journal of Biochemistry Beta-galactosidase-neuraminidase deficiency: restoration of beta-galactosidase activity by protease inhibitors -- Suzuki et al. Beta-galactosidase-neuraminidase deficiency: restoration of beta-galactosidase activity by protease inhibitors Neuraminidase was not affected by any of these jb.oupjournals.org /cgi/content/abstract/90/1/271   (3188 words)

LAH Publications #300 - #399 In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene are purified according to their level of beta-galactosidase expression with a fluorogenic beta-galactosidase substrate and fluorescence-activated cell sorting. Detection for inversional activation of this gene is achieved by a fluorogenic assay, termed FACS-Gal, that detects beta-galactosidase activity in viable cells. In the second vector, the activatable gene is the bacterial beta-galactosidase gene (lacZ). herzenberg.stanford.edu /Publications/300-399.htm   (3188 words)

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