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Topic: Biotinylation

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Biotin

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	Metabolic Biotinylation
		Given metabolic biotinylation allows one to tag a number of capsomer proteins with BAPs and then combine these with any biotinylated ligand, this provides a unique platform to compare the functions of a given viruses proteins for targeting and purification.
		As a first example of this approach, we have utilized metabolic biotinylation to directly compare targeted transduction of adenovirus as mediated by its fiber, protein IX, and hexon capsomeres using a variety of biotinylated ligands (Virology 349(2):453-62 2006).
		As such, biotinylated vectors can be used as a platform for combinatorial screening of both cell-targeting ligands and for receptors that are compatible with cell targeting.
	mayoresearch.mayo.edu /mayo/research/barry_lab/metabolic-biotinylation.cfm (906 words)

	Exposure to UV light causes increased biotinylation of histones in Jurkat cells -- Peters et al. 283 (3): C878 -- AJP ...
		Previous studies have suggested that biotinylation of histones is catalyzed by biotinidase (8).
		Biotinylated histones are enriched in transcriptionally inactive chromatin.
		The magnitude by which biotinylation of histones increased in response to UV exposure was quantitatively moderate.
	ajpcell.physiology.org /cgi/content/full/283/3/C878 (4215 words)

	Biotinylation of reactive amino groups in native recombinant human interleukin-1 beta -- Yem et al. 264 (30): 17691 -- ...
		Biotinylation of reactive amino groups in native recombinant human interleukin-1 beta -- Yem et al.
		Biotinylation of reactive amino groups in native recombinant human interleukin-1 beta
		biotinylated rIL-1 beta by C4 reverse phase HPLC resolved a protein
	www.jbc.org /cgi/content/abstract/264/30/17691 (324 words)

	Structure and selectivity in post-translational modification: attaching the biotinyl-lysine and lipoyl-lysine swinging ... (Site not responding. Last check: )
		Wild-type and mutant biotinyl domains were isolated from E.coli BL21(DE3) cells transformed with the relevant plasmid and grown in the presence of either d-biotin (10 mg/l, bio) or DL-lipoic acid (10 mg/l, lip).
		The extent (%) of biotinylation was determined densitometrically and plotted against the quantity (µg) of BPL present in the reaction mixture.
		Murtif, V.L. and Samols, D. Mutagenesis affecting the carboxyl terminus of the biotinyl subunit of transcarboxylase.
	embojournal.npgjournals.com /cgi/content/full/18/10/2673 (6753 words)

	Search for the Autoantibody Immunodominant Region on Thyroid Peroxidase: Epitopic Footprinting with a Human Monoclonal ...
		The extent of biotinylation of TPO and the TPO-TR1.9
		The tryptic fragment eluting at 38 min on reverse-phase HPLC is biotinylated.
		Purification of the biotinylated polypeptide fragment eluting on reverse-phase HPLC at 38 min.
	www.jimmunol.org /cgi/content/full/166/2/1327 (5489 words)

	Method: Biotinylation
		Biotinylation is a rapid method of detecting nucleic acids for use in a Western blot.
		Biotinylation of antigens is used to improve immunoassays.
		CHO B2 cells transfected with alpha5 were washed with buffer and biotinylated for 60 minutes.
	student.biology.arizona.edu /honors2001/group08/method/method3.htm (73 words)

	Protein Biotinylation Kit
		Protein (antigen and antibody) biotinylation is widely used in ELISA/EIA as a detection molecule.
		Biotinylation often requires fine adjustments between the ratios of biotin and conjugating protein.
		It is also possible to coat the ELISA plates directly with the biotinylated proteins and then detecting it streptavidin-HRP or anti-biotin antibodies.
	www.4adi.com /kits/80300.html (467 words)

	ARS \| Publication request: Lysine-8 and Lysine-12 Are Biotinylated on Human Histone H4.
		This study elucidates the lysine residues of Human histone H4 that are the targets for biotinylation and has utilized synthetic peptides to delineate regions of the H4 protein that are biotinylated.
		Substitution of lysine-8 or lysine-12 by alanine or arginine decreased biotinylation, suggesting that these lysines are targets for biotinylation; lysine-8 and lysine-12 are also known targets for acetylation.
		Here we sought to identify amino acid residues that are biotinylated in histone H4, and we sought to determine whether acetylation and methylation of histones affect biotinylation.
	ars.usda.gov /research/publications/publications.htm?seq_no_115=158398 (691 words)

	Perinuclear localization of Na-K-Cl-cotransporter protein after human cytomegalovirus infection -- Maglova et al. 286 ...
		The percent decrease of biotinylated NKCC was calculated from the linear regression data obtained from 2 experiments, as shown in A and C.
		Samples of the biotinylated NKCC protein from mock-infected cells (M) and HCMV-infected cells at various times (6, 24, 48, 72, and 96 h) after viral exposure (H) were applied to the SDS-PAGE.
		Data from 2 identical experiments are presented as the average percentage of the biotinylated NKCC in HCMV-infected cells relative to that found in mock-infected MRC-5 cells (M).
	ajpcell.physiology.org /cgi/content/full/286/6/C1324 (7638 words)

	K8 and K12 are biotinylated in human histone H4 -- Camporeale et al. 271 (11): 2257 -- FEBS Journal (Site not responding. Last check: )
		Biotinylated histone H4 was probed using the antibody to histone H4 (biotinylated at
		Biotinylation of lysine residues in histones is not stereospecific.
		Peptides were probed using an antibody to histone H4, biotinylated at K12 (lanes a and b), preimmune serum (lanes c and d), or streptavidin–peroxidase (lanes e and f).
	content.febsjournal.org /cgi/content/full/271/11/2257 (3807 words)

	BK101 Biotinylation Kits
		The ester provides a carbonyl carbon adjacent to a labile ester linkage as a target for primary amine side chains of accessible lysine residues, joining the biotinamidocaproate to the protein via an amide bond.
		Kit includes all reagents necessary for biotinylation and for determination of biotin/protein molar ratio.
		When conjugated to enzymes or fluorchromes avidin provides a means of identifying biotinylated compounds via enzymatic conversion of substrate to form a visible product or detection of fluorescence by spectrophotometry or flow cytometry.
	www.sigmaaldrich.com /catalog/search/ProductDetail?ProdNo=BK101&Brand=SIGMA (407 words)

	Complications in cell-surface labelling by biotinylation of Candida albicans due to avidin conjugate binding to ...
		Complications in cell-surface labelling by biotinylation of Candida albicans due to avidin conjugate binding to cell-wall proteins -- Masuoka et al.
		biotinylated bands began to be lost at the higher sample dilutions.
		Cole, S. R., Ashman, L. and Ey, P. Biotinylation: an alternative to radioiodination for the identification of cell surface antigens in immunoprecipitates.
	mic.sgmjournals.org /cgi/content/full/148/4/1073 (3232 words)

	Determination of the Extent of Protein Biotinylation by Fluorescence Binding Assay
		The extent of biotinylation was determined by complete acid hydrolysis or by enzymatic digestion using proteinase K to release biotin from the biotinylated proteins, followed by sensitive detection of biotin using a coupled HPLC-binding assay system.
		The extent of biotinylation determined by this method was compared with the values obtained by a conventional colorimetric method that is based on the displacement of the dye 4-hydroxyazobenzene-2-carboxylic acid (HABA) from the binding sites of avidin.
		It was found that, because the described method determines the amount of liberated biotin after hydrolysis, it does not suffer from steric hindrance problems associated with the ability of biotin on a protein surface to displace HABA from avidin.
	pubs.acs.org /cgi-bin/abstract.cgi/bcches/1997/8/i01/abs/bc960080p.html (253 words)

	Synaptic-like Microvesicles of Neuroendocrine Cells Originate from a Novel Compartment That Is Continuous with the ...
		biotinylated at 18°C was present in these subplasmalemmal membranes.
		For biotinylation at 18°C, the 37°C rinse was removed, the dishes were rinsed with 5 ml of PBS
		biotinylated for 30 min at 18°C and chased for 5 min at 18°C were
	www.jcb.org /cgi/content/full/137/2/445 (9333 words)

	In Vitro Enzymatic Biotinylation of Recombinant Fab Fragments through a Peptide Acceptor Tail (Site not responding. Last check: )
		We describe the site-specific enzymatic biotinylation of recombinant anti-estradiol Fab fragments through a 13 amino acid acceptor peptide translationally fused to the C-terminus of the Fd chain.
		After the removal of nonbiotinylated Fab fragments by monomeric avidin chromatography, the overall yield of biotinylated Fab was 40%.
		The site-specifically biotinylated Fab fragments (BioFab) were tested in streptavidin-coated microtitration wells, to which they were shown to bind linearly with respect to the amount of BioFab added, specifically as indicated by biotin inhibition, and tightly with a half-life of several days.
	pubs.acs.org /cgi-bin/abstract.cgi/bcches/1998/9/i06/abs/bc9800217.html (272 words)

	Biotinylation of Tyramine (Site not responding. Last check: )
		The solution was gently rocked overnight in the dark at RT and filtered through a.2µm filter.
		One ml aliquots of this biotinylated tyramine (BT) were frozen at -75 Celcius.
		Following the application of anti-digoxigenin HRP labeled antibody at a concentration of 1:100 the sections were washed in.1MPBS for 3 x 10 minutes at RT.
	www.uchsc.edu /rmtsc/finger/protocol/Biotinyltyramide.html (136 words)

	Rat kidney inner medulla (organge box, Fig). LKEM, DIR, NHLBI, NIH
		The fixation of lipids prior to protein biotinylation was necessary to ensure apical protein biotinylation.
		Blue and red food dyes were used in the perfusates to visualize fluid changes in the IMCDs.
		The biotinylation was nevertheless confined to the perfused IMCDs as indicated by IMCD marker protein AQP2 staining (Fig.
	dir.nhlbi.nih.gov /papers/lkem/IMCDApicalBiotinylation.aspx (270 words)

	Biotinylation of K12 in Histone H4 Decreases in Response to DNA Double-Strand Breaks in Human JAr Choriocarcinoma Cells ... (Site not responding. Last check: )
		Biotinylation of K12 in Histone H4 Decreases in Response to DNA Double-Strand Breaks in Human JAr Choriocarcinoma Cells -- Kothapalli et al.
		Biotinylation of K12 in histone H4 decreased by 50% as early as 10–20 min after initiation
		Decreased biotinylation of K12 of histone H4 was specific for
	nutrition.org /cgi/content/abstract/135/10/2337 (229 words)

	Avidity - Biotin AviTag Applications
		Because the biotinylation is performed enzymatically, the reaction conditions are very gentle and the labeling is highly specific.
		In addition, it should be noted that the Biotin AviTag has only one fifth of the bulk of alternative biotinylation tag sequences that are over 85 amino acid residues long; an important consideration if steric problems are to be minimized.
		Fast and efficient in vitro biotinylation is achieved using Avidity's highly purified Biotin Ligase that comes complete with our exclusive reaction mix.
	www.avidity.com /apps.html (667 words)

	Biotin Supply Affects Expression of Biotin Transporters, Biotinylation of Carboxylases and Metabolism of Interleukin-2 ... (Site not responding. Last check: )
		Biotin Supply Affects Expression of Biotin Transporters, Biotinylation of Carboxylases and Metabolism of Interleukin-2 in Jurkat Cells -- Manthey et al.
		Biotin Supply Affects Expression of Biotin Transporters, Biotinylation of Carboxylases and Metabolism of Interleukin-2 in Jurkat Cells
		biotinylation of proteins, gene expression and metabolism of
	nutrition.org /cgi/content/abstract/132/5/887 (608 words)

	Z-lab Protocol : Biotinylation of Surface Receptors
		Incubate plates with 2.5 mL biotinylation solution/ 6 cm plate for 30' at 4 degC.
		NOTE: To increase signal, this reaction may also be performed at RT.
		Quench biotinylation reaction by rinsing with Tris-buffered saline (10 mM Tris pH 7.4, 154 mM NaCl) 3x.
	www.ucsf.edu /vonzast/biotin.html (559 words)

	Biotinylation Reagents for Protein Research
		Soltec Ventures offers a comprehensive listing of biotinylation reagents for protein research.
		Listed will be the structure, properties and references to important protein research references.
		Keep in mind this custom service is available once production quantities are needed.
	www.soltecventures.com /biotinylation.htm (75 words)

	Antibody Labeling Kits (Biotinylation and HRP conjugation) & Western Blot Reagents and Kits
		Biotinylation of antigens/antibody is routinely performed to enhance the sensitivity of immunoassays by using Streptavidin-based detection methods.
		The kit provides all necessary reagents and buffers to successfully biotinylate proteins in 1 hr using a long arm Biotin derivative *-aminohexanoyl-Biotin-N-Hydroxysuccinimide Ester) to generate more flexible biotinylated-protein conjugates.
		This kit provides all necessary reagents (Blocking Buffer/antibody diluent, Wash buffer Concn.; a choice of anti-mouse/human/goat/sheep/rabbit/chicken IgG-HRP Conjugate, TMB Substrate except primary antibody and preblotted antigens on nitrocellulose sheet) to perform Western Blots.
	www.4adi.com /kits/westreag.html (486 words)

	TCR-Induced Transmembrane Signaling by Peptide/MHC Class II Via Associated Ig-alpha /beta Dimers -- Lang et al. 291 ...
		Both cells and beads were washed extensively before incubating them together for 3 hours (6 hours for late time point) at 37°C to allow conjugate formation.
		The biotinylation of the soluble TCR was carried out as previously described for biotinylation of soluble MHC molecules [F. Crawford, H. Kozono, J. White, P. Marrack, J. Kappler, Immunity 8, 675 (1998)].
		(upper panels) and M12.g3r (lower panels) cells was achieved using biotinylated 14-4-4 (antibodies against I-E) and FITC streptavidin (in green).
	www.sciencemag.org /cgi/content/full/291/5508/1537/DC1 (710 words)

	Avidity - Frequently Asked Questions
		For mammalian or insect cell expression, we recommend a protease inhibitor cocktail containing leupeptin, pepstatin and PMSF to the biotinylation reaction mix.
		There are three biotinylated proteins in yeast and four biotinylated proteins in mammalian cells, but we have no information whether the E.
		In vitro biotinylation of BSA has been reported, but the reaction is orders of magnitude less efficient than the biotinylation reaction of the AviTag.
	www.avidity.com /faq.html (462 words)

	Site-specific biotinylation of colicin Ia. A probe for protein conformation in the membrane -- Qiu et al. 269 (10): ...
		Site-specific biotinylation of colicin Ia. A probe for protein conformation in the membrane -- Qiu et al.
		Site-specific biotinylation of colicin Ia. A probe for protein conformation in the membrane
		cysteine was biotinylated chemically, so that it could serve as a target
	www.jbc.org /cgi/content/abstract/269/10/7483 (757 words)

	Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic ...
		Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice -- de Boer et al.
		Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice
		F. HOSTERT, and J. Isolation and Characterization of Hematopoietic Transcription Factor Complexes by in Vivo Biotinylation Tagging and Mass Spectrometry
	www.pnas.org /cgi/content/short/100/13/7480 (1009 words)

	Biotinylation Reagent Selection Guide
		We can help you choose a suitable biotinylation reagent for your application.
		To choose a suitable biotinylation reagent for your application, select the required reagent features listed below.
		If the specific feature is not relevant to your application, selecting "No Specific Requirement" will expedite your search.
	www.piercenet.com /products/browse.cfm?fldID=48842E1D-F817-4EFD-AC41-75959543B030 (94 words)

	Research Publications Menu
		Mannanoligosaccharides in fish nutrition: Effects of dietary supplementation on growth and gastrointestinal villi structure in Gulf of Mexico Sturgeon..
		Dietary carbamazepine administration decreases liver pyruvate carboxylase activity and biotinylation by decreasing protein and mRNA expression in rats.
		Effects of implant regimens (trebolone acetate-estradial) and vitamin D3 on fresh beef color and quality.
	fas.ifas.ufl.edu /Report/ResearchPublications/AnimalSciences.html (1993 words)

	Invitrogen - Molecular Probes - Chapter 4 - Biotin Derivatives and Haptens
		Figure 4.3 — ELISA-type assay comparing the binding capacity of biotinylated BSA and goat anti–mouse IgG
		Figure 4.5 — Identification of cell-surface proteins in Jurkat cells labeled with the FluoReporter Cell-Surface Biotinylation Kit
		Figure 4.12 — Motor neuron in a three-day-old chick embryo labeled with lysine-fixable biotinylated dextran
	probes.invitrogen.com /handbook/sections/0400.html (185 words)

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