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Topic: Bisulfite

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	621. Sulfur dioxide and sulfites (WHO Food Additives Series 21)
		Bisulfite at a concentration of 0.1 M was non-mutagenic to E.
		The cleavage of DNA by the oxygen-dependent reaction of bisulfite.
		Hoppe, J.O. & Goble, F.C. The intravenous toxicity of sodium bisulfite.
	www.inchem.org /documents/jecfa/jecmono/v21je15.htm (11593 words)

	KODAK: H-24 Module 3 (Site not responding. Last check: 2007-10-20)
		The theoretical consumption of iodine by sulfite, bisulfite, and thiosulfate is a close approximation of the actual consumption (see Reducing Capacity of Fixer Constituents) The "theoretical" Hypo Index is calculated from a consideration of the equivalent weights of ammonium thiosulfate, sodium sulfite, sodium bisulfite, and sodium thiosulfate pentahydrate.
		Photographic-grade sodium bisulfite is a mixture of sodium sulfite, sodium metabisulfite and water.
		Its purity, as determined by an iodine titration and expressed as sodium bisulfite, is approximately 107.5 percent with a minimum assay of 105 percent.
	www.kodak.com /US/en/motion/support/processing/h243/h2403ecr1308.shtml (872 words)

	Diversity of Dissimilatory Bisulfite Reductase Genes of Bacteria Associated with the Deep-Sea Hydrothermal Vent ...
		Abundance of clone families in the library of bisulfite reductase gene fragments amplified by PCR from the microbial community associated with the dorsal surface of A.
		bisulfite reductase alpha subunit in the database (nonredundant
		with the alpha subunits of the dissimilatory bisulfite reductases
	aem.asm.org /cgi/content/full/65/3/1127 (3486 words)

	ASBC Journal 1999 - Characterization of the Reaction of Bisulfite with Unsaturated Aldehydes in a Beer Model System ... (Site not responding. Last check: 2007-10-20)
		The mechanism and chemical equilibria of addition of bisulfite to unsaturated aldehydes were investigated using NMR spectroscopy.
		Preparative analytical procedures to liberate bisulfite were unable to revert the addition to the double bond.
		The irreversible nature of bisulfite addition to the double bond, and the stability of such adducts, did not support the generally accepted mechanism of the release of unsaturated aldehydes from non-volatile species.
	www.asbcnet.org /journal/abstracts/1999/0908-02a.htm (456 words)

	AllRefer.com - glycerol (Organic Chemistry) - Encyclopedia
		Glycerol is also synthesized on a commercial scale from propylene (obtained by cracking petroleum), since supplies of natural glycerol are inadequate.
		Glycerol can also be obtained during the fermentation of sugars if sodium bisulfite is added with the yeast.
		Glycerol is widely used as a solvent; as a sweetener; in the manufacture of dynamite, cosmetics, liquid soaps, candy, liqueurs, inks, and lubricants; to keep fabrics pliable; as a component of antifreeze mixtures; as a source of nutrients for fermentation cultures in the production of antibiotics; and in medicine.
	reference.allrefer.com /encyclopedia/G/glycerol.html (269 words)

	[No title] (Site not responding. Last check: 2007-10-20)
		In general, the extracted DNA is further modified by sodium bisulfite and then subjected to a method of promoter methylation analysis that involves the use of bisulfite-modified DNA.
		CpG retrieval, however, differs from antigen retrieval in that CpG retrieval recovers the cytosines that are otherwise unmodifiable by sodium bisulfite, as opposed to antigen retrieval that retrieves protein antigen that are otherwise undetectable by antibody due to formalin-induced cross-links.
		cytosines resistant to bisulfite modification varied among different samples and this was probably due to variations in the duration of formalin fixation.
	www.wipo.int /cgi-pct/guest/getbykey5?KEY=03/83107.031009&ELEMENT_SET=DECL (3962 words)

	Toxicologic Review of Selected Chemicals (Site not responding. Last check: 2007-10-20)
		Sodium bisulfite is a white crystalline powder and has an odor like that of sulfur dioxide.
		The ACGIH reports that sodium bisulfite is an eye, skin, and mucous membrane irritant; acute exposures have resulted in mild eye and respiratory effects (ACGIH 1986/Ex.
		Although cases of severe, and even lethal, allergic reactions to this material have been documented from the use of sodium bisulfite as a food additive, OSHA does not believe that there is sufficient information to use as a basis for an exposure limit to protect against inhalation-induced allergic reactions.
	www.cdc.gov /niosh/pel88/7631-90.html (403 words)

	[No title] (Site not responding. Last check: 2007-10-20)
		Preferably said sulfite, bisulfite or meta bisulfite is that of an alkali metal.
		A method of claim 9 wherein said bisulfite is a meta bisulfite.
		A method of any one of claims 8 to 10 said sulfite, bisulfite or meta bisulfite is that of an alkali metal.
	www.wipo.int /cgi-pct/guest/getbykey5?KEY=01/17380.010315&ELEMENT_SET=DECL (1488 words)

	Water Technologies: Bottled Water - Pretreatment: Chemical (Site not responding. Last check: 2007-10-20)
		However, sodium bisulfite cannot serve as a substitute for a carbon filter because it has no effect on organics.
		Sodium bisulfite systems are appropriate for water sources with organics that cause no foaming in the evaporation process.
		Injecting enough sodium bisulfite in the presence of chlorine converts the chlorine to chlorides, but there must be sufficient contact time between the chlorine and sodium bisulfite.
	www.water-technologies.com /Bottled/Pretreatment/Chemical.html (204 words)

	Textile Research Journal: A Novel Perfluoroalkyl-Containing Bisulfite Adduct of Polyisocyanate: Synthesis and Finishing ...
		From the literature [5], we know that the bisulfite adduct of polyisocyanate can be decomposed to reform free isocyanate under heat or alkaline conditions, which is able to react with hydroxyl groups of cellulose or with itself to form polyurethane elastomers.
		We therefore believe that the bisulfite adduct of polyisocyanate will be beneficial for improving the wrinkle recovery angles and strength of cotton fabrics.
		In order to make the bisulfite adduct of polyisocyanate with water and oil repellent properties, F-diol was synthesized and introduced into a polyisocyanate.
	www.findarticles.com /p/articles/mi_qa4025/is_200503/ai_n13616765 (1159 words)

	Textile Research Journal: Applying a Bisulfite Adduct of Polyisocyanate to Cotton Fabrics as a Durable Press Finishing ...
		The bisulfite adduct of polyisocyanate (BAP), designed and synthesized in our laboratory, is applied to cotton fabrics as a durable press finishing agent.
		Nonformaldehyde fabrics with good durable press performance are produced by treatment with 4.0% BAP in combination with 7% citric acid (CA) under conditions of drying 3 minutes at 80°C and curing 2 minutes at 170°C with sodium hypophosphite as a curing catalyst.
		When Petersen [9] described the formation and reactions of isocyanate bisulfite adducts (BAP) in 1949, he noted that they could be used in textile finishing, particularly for multifunctional products, because these would form high molecular weight condensation products on the fibers as a result of an initial aqueous treatment.
	www.findarticles.com /p/articles/mi_qa4025/is_200501/ai_n9478338 (1336 words)

	Eleusis: Purification of Ketones by Sodium Bisulfite (Site not responding. Last check: 2007-10-20)
		It is a somewhat well known fact that sodium bisulfite will add to the carbonyl group of a ketone to form a crystalline addition product.
		The bisulfite addition is a fairly general process, and may be adapted to many different ketones (keeping the molar ratios in proportion), however, there are going to be some ketones which will either not react or react to a low extent (mainly ones in which there is significant steric hindrance to the carbonyl carbon).
		Add 52g sodium bisulfite (NaHSO3,.5moles) to approximately 90mL of distilled water at room temperature with vigorous stirring (slightly more water may be used to get it all into solution).
	www.erowid.org /archive/rhodium/chemistry/eleusis/bisulfite.html (628 words)

	DNA Methylation (Site not responding. Last check: 2007-10-20)
		Tag-Adapted Bisulfite Genomic Sequencing for Fine-Mapping of DNA Methylation
		The standard approach is to use bisulfite chemical conversion of unmethylated cytosine residues, specifically, to uracil, and subsequent substitution with thymidine on subsequent cycles of PCR.
		Methylated cytosines are resistant to bisulfite conversion, and remain cytosines in the PCR.
	www.hrinet.org /techtran/technologies/dna_methylation.htm (647 words)

	B&W Photo - Film & Processing Forum: sodium sulfite v sodium bisulfite
		Sodium bisulfite as a dry chemical agent is really sodium metabisulfite.
		Sodium bisulfite or metabisulfite provides mostly bisulfite ion and sodium ion in a dilute solution.
		Depending on the pH of the solution, which can be adjusted by any of the standard technique, the bisulfite ion is at equilibrium with sulfurous acid or sulfite.
	www.photo.net /bboard/q-and-a-fetch-msg?msg_id=00CGON&unified_p=1 (624 words)

	Sodium bisulfite 26% - Juan Messina S.A. Chemical Industries
		Transparent light yellow to greenish liquid, with a sharp and penetrating smell.
		In terms of chemistry, it is a sodium bisulfite solution with an average concentration of 26%.
		Its main application in industry is controlling the corrosion by removing the dissolved oxygen which is present in water Ilnes such as the secondary recovery of petroleum.
	www.juanmessina.com.ar /ingles/sodiumbisulfite26.htm (144 words)

	Protocol Online: Bisulfite Modification (Conversion) of DNA
		Abstract: Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detecting methylated cytosines using methylation specific PCR (MSP) technique or bisulfite genomic sequencing after PCR amplification with or without cloning.
		Alternatively, 2M sodium metabisulfite can be used instead, which is equivalent to 3.9 M sodium bisulfite in molarity.
		Its purpose is to prevent DNA from strand breakage because of depurination.
	www.protocol-online.org /prot/Detailed/3160.html (856 words)

	Association of tissue-specific differentially methylated regions (TDMs) with differential gene expression -- Song et ...
		by methylation-specific PCR (MSP) and bisulfite genomic sequencing.
		The normal sequence and bisulfite sequence are shown.
		Genomic DNA was treated with sodium bisulfite and then amplified with primers specific for methylated and unmethylated sequences within the Pst6 TDM (CpG1) and a small CpG island just 3' of Pst6 (CpG2).
	www.pnas.org /cgi/content/full/102/9/3336 (3432 words)

	Current Protocols in Food Analytical Chemistry-Table of Contents (Site not responding. Last check: 2007-10-20)
		Polymerized colored anthocyanin-tannin complexes are resistant to bleaching by bisulfite, whereas the bleaching reaction of monomeric anthocyanins will rapidly go to completion.
		The bisulfite bleaching reaction is utilized to generate the various degradation indices.
		The absorption of these compounds are in general not affected by the addition of a bisulfite solution.
	www.wiley.com /legacy/cp/cpfac/facsample.htm (3971 words)

	PCR/Bisulfite Conversion Based PCR Protocols
		The bisulfite reaction reaction was first described in early 1970s and was used by Frommer et al to distinguish between cytosine and 5mCytosine (5mC) in DNA.
		In this reaction, DNA is first treated with sodium bisulfite to convert cytosine residues to uracil in single stranded DNA, under conditions whereby 5mC remains essentially non-reactive.
		All methods share the same procedure of modifying DNA with sodium bisulfite as the first step and subsequently PCR amplification with primers specific for modified DNA.
	www.protocol-online.org /prot/Molecular_Biology/PCR/Bisulfite_Conversion_Based_PCR (277 words)

	Addition of Bisulfite to Aldehydes
		The unshared electron pair of the sulfur atom of bisulfite anion is fitted into the electron gap of the positively polarized carbonyl C atom:
		Aldehydes are isolated from reaction mixtures through its bisulfite derivate.
		The addition compound can be easily split to regenerate aldehyde by treating with dilute mineral acid.
	www.uni-regensburg.de /Fakultaeten/nat_Fak_IV/Organische_Chemie/Didaktik/Keusch/D-Aldehyde-e.htm (190 words)

	X inactivation-specific methylation of LINE-1 elements by DNMT3B: implications for the Lyon repeat hypothesis -- Hansen ... (Site not responding. Last check: 2007-10-20)
		A map of the 500 bp L1 region examined by bisulfite methylation analysis is shown at the top.
		Newly replicated DNA that was labeled with bromodeoxyuridine was isolated from cell cycle-fractionated cells and subject to PCR amplification and Southern blot analysis.
		Bisulfite methylation analysis of the antisense strand was performed
	hmg.oxfordjournals.org /cgi/content/full/12/19/2559 (5289 words)

	Cape May Foods Recalls Canned Shellfish Because of Label Error (Site not responding. Last check: 2007-10-20)
		The Scungilli was labeled without reference to the presence of sodium bisulfite, a common additive used to preserve color.
		Some people are allergic to sodium bisulfite and, accordingly, the product label should include reference to the presence of sodium bisulfite so that consumers can be aware and take appropriate precautions.
		If a consumer is not allergic to sodium bisulfite, they may use the product as anticipated, the product itself is wholesome and fit for human consumption.
	www.fda.gov /oc/po/firmrecalls/capemay12_01.html (327 words)

	Bleaching Lumber (Site not responding. Last check: 2007-10-20)
		This method works well.The soduim bisulfite is on its way and I was wondering if the sodium hydrosulfite was generally similar to soduim bisulfite?
		Sodium bisulfite is an old name for sodium hydrogen sulfite -- the "bi" in the older literature standing for "hydrogen".
		I'm a bit confused by your equating this substance with "laundry bleach" which is typically sodium hypochlorite (an oxidizing agent, as is potassium permanganate).
	www.newton.dep.anl.gov /askasci/chem00/chem00675.htm (253 words)

	Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients ...
		Bisulfite modification of DNA samples (up to 1 µg) was
		Genomic DNA was treated with bisulfite and amplified for a specific region of interest containing multiple CpG islands by PCR.
		The average signal intensity was taken from two spots on four slides proceeded in parallel.
	nar.oxfordjournals.org /cgi/content/full/33/5/e46 (3333 words)

	General Chemistry Online: FAQ: Simple compounds: What are the sulfite and bisulfite ions, and what are they used for? (Site not responding. Last check: 2007-10-20)
		What are the sulfite and bisulfite ions, and what are they used for?
		What are some common sulfite and bisulfite compounds?
		What are the structures of the sulfite and bisulfite ions?
	antoine.frostburg.edu /chem/senese/101/compounds/faq/sulfite-bisulfite.shtml (741 words)

	SODIUM BISULFITE
		Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.
		Sodium Bisulfite [7631-90-5]: Oral rat LD50: 2000 mg/kg.
		Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility.
	www.jtbaker.com /msds/englishhtml/s3074.htm (1105 words)

	RTC # 02-348
		Sodium bisulfite is then metered into the sidestream via a temporary system consisting of a bisulfite storage tank and metering pump.
		The deliverables from the consultant will include a design of a sodium bisulfite system, plans and specifications suitable for construction, an operations manual, a training program for city staff, and final record drawings.
		It is recommended that Council award a contract to Kennedy Jenks Consultants for engineering design and construction support services for the automatic sodium bisulfite system in substantially the same form as the attached draft and in an amount not to exceed $83,400.
	sunnyvale.ca.gov /200208/rtcs/02-348.asp (345 words)

	UCLA/MCDB/Jacobsen Lab/Bisulfite genomic sequencing (Site not responding. Last check: 2007-10-20)
		5) Dissolve 40.5g of sodium bisulfite (Fisher S654-500) in 80mls of water with slow stirring to avoid aeration.
		Note, because it takes >10min for the sodium bisulfite** to dissolve, this step should be started at the same time as step 3 is started.
		Do this in the PCR machine, so that the denatured DNA is still at 39C.
	www.mcdb.ucla.edu /Research/Jacobsen/BisulfiteGenomicSequencing.html (679 words)

	Sodium bisulfite Use
		Sodium bisulfite - Pesticide use statistics for 2003
		Top 50 Crops and Sites for for Sodium bisulfite use in California in 2003
		Regional Use for Sodium bisulfite on All Sites in 2003
	www.pesticideinfo.org /Detail_ChemUse.jsp?Rec_Id=PC34354 (890 words)

	Quantitative Analysis of Tumor-derived Methylated p16INK4a Sequences in Plasma, Serum, and Blood Cells of ...
		was the percentage of bisulfite converted unmethylated and methylated
		Bisulfite modification was conducted based on the principle
		The completeness of bisulfite conversion was measured by calculating
	clincancerres.aacrjournals.org /cgi/content/full/9/3/1047 (3892 words)

	M. D. Anderson Cancer Center - DNA Methylation in Cancer - Protocols - Bisulfite Treatment of DNA
		Anderson Cancer Center - DNA Methylation in Cancer - Protocols - Bisulfite Treatment of DNA
		Incubate at 37°C for 10 minutes (to create single stranded DNA).
		l freshly prepared 3M Sodium bisulfite (Sigma S-8890), prepared by adding 1.88 gm of sodium bisulfite per 5 ml of H
	www.mdanderson.org /departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2 (258 words)

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