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Topic: Blood agar

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Agar plate

Agar

Petri dish

Acanthamoeba

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Viral plaque

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	Blood agar (Site not responding. Last check: 2007-10-08)
		Blood agar is made by enriching the nutrient agar with horse blood.
		Blood agar plates (BAP) are used both as enriched media and to differentiatebetween individuals on the basis of their ability to produce hemolysins,...
		CDC Anaerobe Blood Agar with Kanamycin and Vancomycin (KV)...
	www.cappuccinoworld.com /blood+agar.html (965 words)

	Learn more about Agar plate in the online encyclopedia. (Site not responding. Last check: 2007-10-08)
		Once dissolved the agar needs to be sterilised by pouring it into a conical flask, then sealing the top with a cotton wool wad, and finally covering the cotton wool with a loose layer of aluminium foil.
		The sterile agar is then allowed to cool to 50°C. This is just above the setting point, Pouring at this temperature helps prevent condensation forming on the lid.
		With this method, a small amount of sample is placed on the side of the agar plate (either with a swab, or a drop from an inoculating loop if it's a liquid).
	www.onlineencyclopedia.org /a/ag/agar_plate.html (1134 words)

	Hemolysis on Blood Agar (Site not responding. Last check: 2007-10-08)
		Hemolysis on blood agar is used for the preliminary or confirmatory identification of many types of clinically important bacteria.
		Alpha-hemolysis is a greenish discoloration of the blood agar surrounding a bacterial colony; it is a characteristic of Streptococcus pneumoniae.
		C in the presence of 5% CO This results in an overall brownish discoloration of the blood agar, from its original blood-red hue.
	web.indstate.edu /thcme/micro/hemolys.html (219 words)

	Blood Agar (Site not responding. Last check: 2007-10-08)
		One advantage of sheep blood is its inhibitory effect on the growth of bacteria such as Haemophilus haemolyticus, the colonial morphology of which may resemble that of the b-hemolytic streptococci.
		Isolation/Maintenance: Inoculate the specimen onto the surface of the blood agar plate and streak for isolation.
		Growth on the blood agar indicates the bacteria requires only the X factor, whereas, growth only near the S.
	dentistry.ouhsc.edu /intranet-WEB/Courses/DMI_8351/Blood_Agar.html (419 words)

	[No title] (Site not responding. Last check: 2007-10-08)
		blood agar, chocolate agar, supplemented blood agar for anaerobes and THIO broth blood agar, chocolate agar, and supplemented blood agar for anaerobes blood agar, chocolate agar, and potato dextrose agar category a 1995 The work-up of bacterial isolates for orbital cellulitis is the inoculation of blood agar and chocolate agar.
		blood agar, chocolate agar, and potato dextrose agar category c 1995 Bilateral bacterial cultures are mandatory in diagnosing conjunctivitis, even in cases of suspected unilateral conjunctivitis.
		Blood cultures are not essential in the case of endophthalmitis.
	www.meddean.luc.edu /lumen/bbs/infdis/chap23 (891 words)

	Agar -- Facts, Info, and Encyclopedia article (Site not responding. Last check: 2007-10-08)
		Chemically, agar is a polymer made up of subunits of the sugar galactose; it is a component of the algae's cell walls.
		Dissolved in hot water and cooled, agar becomes (An edible jelly (sweet or pungent) made with gelatin and used as a dessert or salad base or a coating for foods) gelatinous; its chief use is as a culture medium for (Click link for more info and facts about microbiological) microbiological work.
		The basic agar formula can be used to grow almost all microbes; however, other more specific nutrient agars are available, e.g., blood agar, which is generally combined with horse blood, and can be used to detect the presence of haemophoragic micro-organisms such as E.coli O:157 H:7 (the bacteria digest the blood, turning the plate clear).
	www.absoluteastronomy.com /encyclopedia/a/ag/agar.htm (218 words)

	Cell culture (Site not responding. Last check: 2007-10-08)
		blood agar (BA) plates (BAP) : an enriched non-selective trypticase soy agar medium containing beef heart infusion base, peptone, and sodium chloride, autoclaved and enriched by the addition of 5% sterile defibrinated blood, used for primary plating and subculturing, especially to determine bacterial hemolysis.
		blood agar except that the blood is laked (hemolyzed) by freezing and thawing.
		Petragnani culture medium : a agar culture medium containing bovine milk, potato flour, potato, whole egg and egg yolk, and malachite green, for the culture of tubercle bacilli; the medium is solidified by heat coagulation of the egg.
	focosi.altervista.org /cellculture.html (9125 words)

	Bordetella bronchiseptica on blood agar (Site not responding. Last check: 2007-10-08)
		The colonies formed are in the region of 1mm in diameter and develop a rough surface that is pitted like beaten metal.
		bronchiseptica forms larger colonies than on BG agar, and the colonies are greyish-white, somewhat flat and lack the metallic appearance that they have on BG agar.
		bronchiseptica may produce hazy zones of haemolysis on BG agar.
	www.felinebb.info /disease/microbiology/figure1.asp (58 words)

	Blood Agar (Site not responding. Last check: 2007-10-08)
		Purpose Blood agar is used both as an enriched medium for growing fastidious bacteria and as a differential medium.
		Alpha hemolysis refers to the partial lysis of RBC's and hemoglobin and produces a greenish discolration of the blood agar around the colonies.
		A common mistake is to look at the color of the bacteria, rather than the agar.
	www.austin.cc.tx.us /microbugz/19blood.html (185 words)

	Antimicrobial Resistance of S. pneumoniae and H. influenzae
		For laboratories handling blood culture specimens, technicians must be able to identify possible bacterial growth in the culture bottles, isolate bacteria on solid media, and subculture isolates as described in detail in the laboratory section of the manual.
		Blood cultures will be positive for a bacterial pathogen in approximately 10% to 35% of children with pneumonia (3,16,17,18).
		Blood cultures from young children should be diluted to 1-2 ml of blood in 20 ml of broth (1:10 to 1:20).
	who.int /child-adolescent-health/New_Publications/CHILD_HEALTH/bact.htm (10161 words)

	AGAR (Site not responding. Last check: 2007-10-08)
		The agar is dissolved in the boiling water.
		In the particular case of blood agar the bacteria are getting the majority of their nutrients from the blood (could be sheep' blood), other nutrients which are necessary for growth may be added to the agar.
		As for temperature, if you are growing bacteria on blood agar plates it usually implies that you are trying to culture bacteria which live in an animal.
	agar.net (869 words)

	Bacterial cultivation MCQ
		When some bacteria are grown on a culture medium containing blood they produce a change in the appearance of the blood in the area around the colony.
		MacConkey Agar has been used because it is a good and useful example of a growth medium which is both a selective and ani indicator medium.
		MacConkey Agar contains bile salts to inhibit non-enteric bacteria, a mixture of peptides, as a source of nitrogen, various minerals and vitamins needed to support bacterial growth and a pH indicator called Neutral Red which is pink in acid condiditons and yellow in alkali.
	www.ncl.ac.uk /dental/oralbiol/oralenv/mcqs/genmicro/cultivation2.htm (1549 words)

	Encyclopedia: Sputum
		Red blood cells (erythrocytes) are present in the blood and help carry oxygen to the rest of the cells in the body Blood is a circulating tissue composed of fluid plasma and cells (red blood cells, white blood cells, platelets).
		Fibrin is a protein involved in the clotting of blood.
		An agar plate streaked with microorganisms isolated from a deep-water sponge.
	www.nationmaster.com /encyclopedia/Sputum (501 words)

	CDC - Ruling Out Bacillus anthracis
		Presumptive identification is based on demonstrating a lack of β-hemolysis on sheep (2,3) or horse (3) blood agar plates and the organism’s lack of motility (2,3).
		At 24 h, strong β-hemolysis was produced by 55 (76%), 41 (57%), and 55 (76%) strains on human, sheep, and horse blood agar plates, respectively, while 6 (8%), 10 (14%) and 7 (10%) strains demonstrated weak β-hemolysis (Table).
		Finally, β-hemolysis results obtained with horse and human blood agar plates, although not identical, were usually in agreement and differed from those obtained with sheep blood agar plates (data not shown).
	www.cdc.gov /ncidod/EID/vol10no4/03-0544.htm (2214 words)

	BSCI424 Laboratory Media
		Blood Agar (BAP): consists of a basal medium such as TSA enriched with 5% defibrinated sheep blood or in some locations, horse blood.
		Motility-Indole-Ornithine Agar: motility is indicated by the character of the growth in the butt of this tube.
		Simmons Citrate Agar: utilization of citrate as the sole source of carbon is indicated by the medium turning a deep blue color because of an alkaline reaction.
	www.life.umd.edu /classroom/bsci424/LabMaterialsMethods/BSCI424Media.htm (1564 words)

	3954staphylococcus.html
		A sugar-free blood agar base is recommended with the addition of 5% sheep or rabbit blood.
		Hemolytic activity on blood agar is the way we detect the production of certain toxins, hemolysins, or lysins (all of these names refer to the same material) by staphylococci.
		This observation is performed on the radially streaked blood agar plate with the knowns and the isolates.
	www.tarleton.edu /~jkonvicka/3954staphylococcus.html (2378 words)

	3954streptococcus.html
		Also stab each organism into the agar using the loop (at approximately a 45 degree angle) in other words, puncture the agar a couple of times in an unused portion of the streaked sector.
		Streptococcus pyogenesisolation is aided by the use of blood agar plates with stabs to allow subsurface growth, giving improved conditions for this organism to give its typical beta-hemolysis.
		A plate of blood agar should accomodate about six organisms (three on one side and three on the other) for testing.
	www.tarleton.edu /~jkonvicka/3954streptococcus.html (1930 words)

	Optochin Revisited: Defining the Optimal Type of Blood Agar for Presumptive Identification of Streptococcus pneumoniae ...
		at 35°C in 5% CO on Columbia agar with 5% sheep blood.
		pneumoniae on COL-, TSA-, and MH-sheep blood agar incubated in air and in CO The current NCCLS criteria for the presumptive identification of S.
		blood agar, as none of the isolates produced optochin zones of
	jcm.asm.org /cgi/content/full/36/3/833 (1156 words)

	Gonorrhoea : Methods in diagnosis (Contd. 2)
		The GC agar base medium is prepared at single strength which is half the strengh recommended by the manufacturer, since the addition of equal volume of 2% haemoglobin is replaced by adding lysed blood.
		For LBA the GC agar base medium is prepared at single strength, which is half the strength recommended by the manufacturer if other additives are not being included.
		If the numbers are high such that confluent or semi-confluent growth is evident, then an estimate of growth can still be made by examining both the amount of growth on the main inoculum as well as the extent of growth on the plate, provided the standard procedure was used for streaking the inoculum.
	w3.whosea.org /bct/book33/1a.htm (2781 words)

	BBL Images
		CIN (Cefsulodin-Irgasan-Novobiocin) Agar is superior to MacConkey Agar as a primary plating medium for the isolation of Yersinia spp.
		CDC Anaerobe Blood Agar is the preferred formulation for the isolation and cultivation of obligately anaerobic bacteria.
		BBL Mycosel Agar is a selective medium for the isolation and cultivation of pahtogenic fungi, both yeasts and molds, from highly contaminated specimens.
	www.troybio.com /images/Product_Images_BBL/BBL.htm (6083 words)

	gram stains using blood agar (Site not responding. Last check: 2007-10-08)
		The susceptibility agar medium must be of a...
		Diagnosis: cultures and gram stains of blood, sputum, CSF and...
		is that the red blood cells in the...
	www.all-stain-removal.info /gramstainsusingbloodagar (447 words)

	Culture of Bartonella quintana and Bartonella henselae from Human Samples: a 5-Year Experience (1993 to 1998) -- La ...
		Growth of bartonellae is slow, since when blood agar is used (16, 34, 40, 60), primary isolates are typically obtained
		For patients with endocarditis, inoculation of blood culture broth and heparinized blood onto shell vial was significantly
		blood, (ii) the presence in blood of a soluble and labile factor
	jcm.asm.org /cgi/content/full/37/6/1899 (6191 words)

	Scarlet fever
		The Group A streptococci are; hemolytic bacteria, which means that the bacteria have the ability to lyse or break red blood cells.
		A throat swab from the infected person is brushed over a nutrient gel (a sheep blood agar plate) and incubated overnight to detect the presence of hemolytic bacteria.
		A petri dish filled with a nutrient gel containing red blood cells that is used to detect the presence of streptococcal bacteria in a throat culture.
	www.healthatoz.com /healthatoz/Atoz/ency/scarlet_fever.jsp (1387 words)

	BBL Prepared Microbiology Media
		Sabouraud Dextrose Agar with Lecithin and Polysorbate 80
		Blood Agar Slants (Trypticase Soy Agar with Defibrinated Sheep Blood Slants) (Ctn.
		Blood Agar Slants (Trypticase Soy Agar with Defibrinated Sheep Blood Slants) (Pkg.
	www.voigtglobal.com /PML_Standard_Plates.htm (1914 words)

	[No title] (Site not responding. Last check: 2007-10-08)
		Do isolation streak onto blood agar and incubate at 37o C for 48 hours.
		Many of these are catalase negative, alpha hemolytic on blood agar, A disk negative, not dissolved by bile salts, and are CAMP negative.
		You can pick an isolated colony from the second blood agar streak plate, inoculate into a TSB tube and incubate for 24 hrs (or more) at 37o C. Use this culture to swab the surface of a Mueller-Hinton plate, using a sterile cotton-tipped swab.
	science.kennesaw.edu /~ddavis/4460/FLOWCHART.doc (422 words)

	Viral plaque (Site not responding. Last check: 2007-10-08)
		Here we have a sample of plaques formed by viruses within a blood agar medium.
		The red region is the solidified blood agar, while the white spots are the plaques.
		The viruses spread through the agar, destroying the blood cells within the agar and generating these characteristic spots.
	lymphocytes.741.com /plaque.html (102 words)

	Production by Bacillus pumilus (MSH) of an antifungal compound that is active against Mucoraceae and Aspergillus ...
		agar, nutrient agar, trypticase soy agar and Mueller–Hinton
		The stability of the compound in agar was determined by preparing
		equivalent in diameter to that on sheep-blood agar.
	jmm.sgmjournals.org /cgi/content/full/52/1/69 (2389 words)

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