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BioMed Central | Full text | Conservation of the COP9/signalosome in budding yeast This phenotype, which resembles that of fission yeast csn mutants, is due to a biochemical defect in deneddylation that is complemented by wild-type cell lysate and by purified human CSN in vitro. In a recent large-scale survey of budding yeast protein complexes [28], five proteins co-purifying in a stable complex with Rri1p/Csn5p were identified by tandem mass spectrometry. Domain structure of budding yeast CSN proteins and csn deletion phenotype(A) Schematic representation of budding yeast genes encoding proteins identified in a stable complex with Rri1p/Csn5p. www.biomedcentral.com /1471-2156/3/15   (3696 words)

SURVEY AND SUMMARY: Analysis of the splicing machinery in fission yeast: a comparison with budding yeast and mammals -- ... yeast and humans that appear to be absent in budding yeast. Slt21p) in budding yeast and p220 in mammals. yeast and humans that are absent in budding yeast. nar.oxfordjournals.org /cgi/content/full/28/16/3003   (4789 words)

Yeasts: Saccharomyces, Cryptococcus, Candida   (Site not responding. Last check: 2007-10-13) Yeasts are fungi that grow as single cells, producing daughter cells either by budding (the budding yeasts) or by binary fission (the fission yeasts). Yeasts grow typically in moist environments where there is a plentiful supply of simple, soluble nutrients such as sugars and amino acids. budding yeast used for bread-making, where the carbon dioxide produced by growth in the dough causes the bread to rise. helios.bto.ed.ac.uk /bto/microbes/yeast.htm   (1310 words)

The Budding Yeast Cell Cycle   (Site not responding. Last check: 2007-10-13) Although the complexity of the regulatory network increases tremendously, the basic mechanism is highly conserved from yeast to man. Budding yeast (Saccharomyces cerevisiae) is the most well-studied model organism for cell cycle research. We show in the budding yeast website and the companion paper that a computational model, built on the consensus picture of regulatory network derived from reductive biochemical studies and genetic manipulations, is largely successful (predicting 120 out of 133 cases) in explaining the phenotypes of currently characterized mutants of the regulatory system. jigcell.biol.vt.edu /budyeastUC.html   (260 words)

Budding Yeast Saccharomyces cerevisiae, the budding yeast, is the common yeast used in baking ("baker's yeast") and brewing ("brewer's yeast"). Budding yeast can live with either two genomes (diploid) or one (haploid). In either case, it reproduces by forming buds (hence the name) by mitosis. users.rcn.com /jkimball.ma.ultranet/BiologyPages/Y/Yeast.html   (343 words)

The World-Wide Web Virtual Library: Yeast Yeast Tet-promoters Hughes Collection containing 800 essential yeast genes for which expression is regulated by doxycycline. Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, from the Yale Genome Analysis Center. The NCRR Yeast Resource Center at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction. www.yeastgenome.org /VL-yeast.html   (1486 words)

Control by Nutrients of Growth and Cell Cycle Progression in Budding Yeast, Analyzed by Double-Tag Flow Cytometry -- ... 1) (i.e., being 2.48 h for the budded phase of the yeast population phase, and duration of the budded phase of diverse cohorts of daughter cells born before, during, and after the transitory state. Structural heterogeneity in populations of budding yeast Saccharomyces cerevisiae. jb.asm.org /cgi/content/full/180/15/3864   (6169 words)

BioMed Central | Full text | Time scale and dimension analysis of a budding yeast cell cycle model The eigenvalues of the Jacobian for the Chen [12] model was calculated during the simulation of the budding yeast cell cycle (Fig. The two cell cycle events during relaxation period R2 are initiation of bud formation and DNA replication, which are coincident during budding yeast cell cycle [3]. The analysis of a budding yeast cell cycle model revealed the presence of autocatalytic excitation and subsequent relaxation periods in the cell cycle and these excitation periods can be connected to major cell cycle transitions. www.biomedcentral.com /1471-2105/7/494   (5376 words)

The MCM2p-binding is crucial for the function of CDC7-DBF4 protein kinase during the initiation of chromosomal DNA ... The MCM2p-binding is crucial for the function of CDC7-DBF4 protein kinase during the initiation of chromosomal DNA replication in budding yeast This study analyzes the biological importance of Mcm2p phosphorylation in budding yeast. Yeast cells carrying the mutant allele mcm2-12SA grow normally and mcm2-12SA fully complements a defect in chromosomal mcm2, but mcm2-12SA is synthetically lethal with temperature-sensitive dbf4-4 and it enhances the temperature sensitivity of dbf4-1, -2, -3 or -5. www.jbc.org /cgi/content/short/M603586200v1   (288 words)

Kinetic Analysis of a Molecular Model of the Budding Yeast Cell Cycle — CellML However, budding yeast are unusual in that they divide asymmetrically. S and M phases are completed before the bud can grow as large as the mother cell and consequently, budding produces a large mother cell and a small daughter cell. A second anomaly of yeast budding is that the cells pass through the S and M phases of the cell cycle without their chromatin condensing into discrete chromosomes. www.cellml.org /models/chen_csikasz-nagy_gyorffy_val_novak_tyson_2000_version01   (664 words)

Microtubule capture by the cleavage apparatus is required for proper spindle positioning in yeast -- Kusch et al. 16 ... The percentage of cells with a nucleus in the vicinity of the bud neck (within 1 µm from the bud neck) is shown. The position of the spindle relative to the bud neck was compared at the beginning and at the end of capture events (n = 89 at bud neck, 40 at bud cortex, 43 at mother cortex). Muhua, L., Karpova, T.S., and Cooper, J.A. A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration. www.genesdev.org /cgi/content/full/16/13/1627   (7705 words)

BioMed Central | Abstract | Characterization of the minimum domain required for targeting budding yeast myosin II to ... Budding yeast has a single myosin II protein, named Myo1. We show that the MLD is a small region in the centre of the tail of Myo1 and that it is both necessary and sufficient for localisation of Myo1 to the yeast bud neck, the pre-determined site of cell division. Our results define the intrinsic determinant for the localization of budding yeast myosin II and show it to be an oligomer of tentatively 3 monomers. www.biomedcentral.com /1741-7007/4/19/abstract   (490 words)

Mutational and hyperexpression-induced disruption of bipolar budding in yeast -- Freedman et al. 146 (11): 2833 -- ... Mutational and hyperexpression-induced disruption of bipolar budding in yeast -- Freedman et al. Mutational and hyperexpression-induced disruption of bipolar budding in yeast axial and bipolar budding polarity (Bender and Pringle, 1989 mic.sgmjournals.org /cgi/content/full/146/11/2833   (5146 words)

Direct Evidence for a Critical Role of Myosin II in Budding Yeast Cytokinesis and the Evolvability of New Cytokinetic ... Direct Evidence for a Critical Role of Myosin II in Budding Yeast Cytokinesis and the Evolvability of New Cytokinetic Mechanisms in the Absence of Myosin II -- Tolliday et al. Direct Evidence for a Critical Role of Myosin II in Budding Yeast Cytokinesis and the Evolvability of New Cytokinetic Mechanisms in the Absence of Myosin II Nicola Tolliday, In the budding yeast Saccharomyces cerevisiae, an actomyosin-based contractile ring is present during cytokinesis, as occurs www.molbiolcell.org /cgi/content/abstract/14/2/798   (607 words)

Sloan-Kettering - Projects: CDK Activation in Fission Yeast Metazoans and budding yeast each have a single CAK, but they are not homologous to one another. In contrast, fission yeast has two CAKs: the Mcs6 complex, which is homologous to the metazoan CAK (Cdk7); and Csk1, which is homologous to budding yeast Cak1. Remarkably, the fission yeast Schizosaccharomyces pombe contains two CAKs — one homologous to Cdk7 of higher eukaryotes and one superficially similar to budding yeast Cak1. www.mskcc.org /mskcc/html/10516.cfm   (521 words)

Candida albicans budding yeast cell: image by Russell Kightley Media FUNGUS or YEAST PICTURE: illustration of a budding yeast cell of the pathogen Candida albicans EXPLANATION OF CANDIDA IMAGE: Candida yeast cells are ovoid and reproduce by budding (bud shown at upper left corner). It can grow by budding (budding yeast cell as pictured), hyphae, or pseudohyphae and it can form resistant spores (chlamydospores). www.rkm.com.au /FUNGI/Candida.html   (378 words)

Research Could Lead To New Drugs For HIV In budding yeast, these mobile elements (called Ty -- or transposable yeast -- elements) encode proteins that are homologs of retroviral proteins. The proteins encoded by Ty elements and the steps of the life cycle in yeast are similar to the proteins encoded by retroviruses and their life cycles in animal cells. Yeast has previously been used as a model to help scientists understand how cancer cells replicate out of control. www.sciencedaily.com /releases/2005/04/050417204306.htm   (601 words)

Budding Yeast Cell Cycle Model Battogtokh, D. and Tyson, J. Bifurcation analysis of a model of the budding yeast cell cycle. Ciliberto, A., Novak, B. and Tyson J.J. Mathematical model of the morphogenesis checkpoint in budding yeast. Chen, K.C., Csikasz-Nagy, A., Gyorffy, B., Val, J., Novak, B. and Tyson, J.J. Kinetic analysis of a molecular model of the budding yeast cell cycle. mpf.biol.vt.edu /research/budding_yeast_model/pp/publications.php?PHPS...   (933 words)

Saccharomyces Genome Database is a scientific database of the molecular biology and genetics of the yeast Saccharomyces cerevisiae, which is commonly known as baker's or budding yeast. In addition to the map itself, Mortimer discovered many of the RAD genes and their epistasis groups, discovered the phenomenon of aging in yeast, and made tetrad dissection facile with the discovery of enzyme extracts that allowed digestion of asci. He was a leading researcher in the field of yeast mitochondrial biogenesis; in recent years his work had focused on nuclear-mitochondrial interactions and inheritance of the mitochondrial chromosome. www.yeastgenome.org   (1039 words)

Cell cycle control of septin ring dynamics in the budding yeast -- Cid et al. 147 (6): 1437 -- Microbiology Rho1 is essential for the proper establishment of bud emergence at a stage subsequent to septin assembly at the pre-bud neck. During bud growth throughout S, G2 and M phases of the cell cycle, the septin ring gradually expands to conform the neck-spanning structure that covers both mother and daughter sides of the constriction, appearing under fluorescence microscopy as the hourglass-shaped ring that is characteristic of metaphase- and anaphase-arrested cells. Sanders, S. and Herskowitz, I. The Bud4p protein of yeast, required for axial budding, is localised to the mother/bud neck in a cell cycle-dependent manner. mic.sgmjournals.org /cgi/content/full/147/6/1437   (7332 words)

GSK-3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down-regulation by Ca2+ in budding yeast -- Mizunuma et al. ...   (Site not responding. Last check: 2007-10-13) Andoh,T., Hirata,Y. and Kikuchi,A. (2000) Yeast glycogen synthase kinase 3 is involved in protein degradation in cooperation with Bul1, Bul2 and Rsp5. Irniger,S., Piatti,S., Michaelis,C. and Nasmyth,K. (1995) Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast. Mizunuma,M., Hirata,D., Miyahara,K., Tsuchiya,E. and Miyakawa,T. (1998) Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast. embojournal.npgjournals.com /cgi/content/full/20/5/1074   (6339 words)

Global Replication-Independent Histone H4 Exchange in Budding Yeast -- Linger and Tyler 5 (10): 1780 -- Eukaryotic Cell density on the endogenous yeast 2µm plasmid (20). Yeast histone H3 and H4 amino termini are important for nucleosome assembly in vivo and in vitro: redundant and position-independent functions in assembly but not in gene regulation. Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1. ec.asm.org /cgi/content/full/5/10/1780   (4489 words)

Apoptosis in budding yeast caused by defects in initiation of DNA replication -- Weinberger et al. 118 (15): 3543 -- ... Apoptosis in budding yeast caused by defects in initiation of DNA replication -- Weinberger et al. Apoptosis in budding yeast caused by defects in initiation of DNA replication The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase jcs.biologists.org /cgi/content/abstract/118/15/3543   (454 words)

Homologous activators of ras in fission and budding yeast. Homologous activators of ras in fission and budding yeast. In the budding yeast Saccharomyces cerevisiae, the CDC25 gene product acts upstream of Ras proteins, but it has not been clear whether CDC25 function is unique to the S. cerevisiae ras pathway. Here we report that the ste6 gene of fission yeast Schizosaccharomyces pombe is a homologue of CDC25: the ste6 gene product and the CDC25 gene product have significant amino-acid similarity in their C-terminal regions. www.pdg.cnb.uam.es /UniPub/iHOP/gp/6915.html   (184 words)

Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast -- Yoshida et al. 161 (5): 889 -- The ... Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast -- Yoshida et al. Fraschini, R., D'Ambrosio, C., Venturetti, M., Lucchini, G., Piatti, S. Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit. Simanis, V. Events at the end of mitosis in the budding and fission yeasts. www.jcb.org /cgi/content/abstract/161/5/889   (438 words)

Mathematical model of the morphogenesis checkpoint in budding yeast -- Ciliberto et al. 163 (6): 1243 -- The Journal of ... Mathematical model of the morphogenesis checkpoint in budding yeast -- Ciliberto et al. Mathematical model of the morphogenesis checkpoint in budding yeast The morphogenesis checkpoint in budding yeast delays progression www.jcb.org /cgi/content/abstract/163/6/1243   (329 words)

A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization -- Butty et ...   (Site not responding. Last check: 2007-10-13) Ayscough,K.R., Stryker,J., Pokala,N., Sanders,M., Crews,P. and Drubin,D.G. (1997) High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor Latrunculin-A. Cell Biol. Leeuw,T., Fourest,L.A., Wu,C., Chenevert,J., Clark,K., Whiteway,M., Thomas,D.Y. and Leberer,E. (1995) Pheromone response in yeast: association of Bem1 with proteins of the MAP kinase cascade and actin. Lew,D.J. and Reed,S.I. (1993) Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins. embojournal.npgjournals.com /cgi/content/full/21/7/1565   (6440 words)

The Encyclopedia of Life Project - Index   (Site not responding. Last check: 2007-10-13) Candida albicans may form a budding yeast, pseudohyphae, germ tubes, true hyphae, and chlamydospores. A number of investigators are interested in germ tube formation because it represents a transition between a yeast and a mold. Generally, either low temperature or pH favors the development of a budding yeast. www.eolproject.org:8080 /alphaindex.jsp?char=c   (513 words)

Chl1p, a DNA Helicase-Like Protein in Budding Yeast, Functions in Sister-Chromatid Cohesion -- Skibbens 166 (1): 33 -- ... Budding yeast Chl1p is 24% identical and 42% similar to human BACH1 (P value of 3e-58). The DEAH motif is underlined in the consensus sequence. Significant sequence similarities (not shown) were also noted between budding yeast Chl1p and the helicase-like protein NHL, a tumor necrosis factor receptor superfamily member (P value 7e-25) and Xeroderma pigmentosum group D complementing protein (P value 1e-24). www.genetics.org /cgi/content/full/166/1/33   (4695 words)

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