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Topic: CDNAs

Related Topics

MRNA

Reverse transcriptase

Polymerase chain reaction

Genetic engineering

Cloning

In the News (Wed 23 Dec 09)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	Transcription-modulatory activities of differentially spliced cDNAs encoding the E2 protein of human papillomavirus ...
		cDNAs representing the a-, a'- and d-type mRNAs of HPV-16 (Fig.
		The polycistronic cDNAs (d, a and a') were transfected at molar amounts equivalent to 2 (lanes 1) and 0·5 µg (lanes 2) of the monocistronic pJS55-E2 plasmid (E2) together with 2 µg (Cf2Th) or 6 µg (C33A) of the LCR–CAT plasmid and 1 µg of the ß-gal plasmid.
		Determination of the transcription-modulatory activities of the monocistronic cDNAs E6I, E7 and E1C towards the HPV-16 LCR (a) and effect of the E1C cDNA on the E2-mediated transcription-modulatory activity (b) in Cf2Th cells.
	vir.sgmjournals.org /cgi/content/full/80/9/2461 (5233 words)

	About Construction of Full-Length cDNAs
		Construction of full-length cDNAs is a central focus in the post-sequence era of the various genome projects, including Arabidopsis.
		However, it is generally agreed upon that a cDNA comprising the entire coding sequence of a protein should be considered worthy for full-length sequencing at high accuracy.
		All three investigators have constructed numerous cDNA libraries during the last 20 years in a variety of vectors that are available to the Arabidopsis community.
	signal.salk.edu /cabout.html (397 words)

	Detailed description of cDNA Libraries (Site not responding. Last check: 2007-10-22)
		The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100.
		The cDNAs were ligated to Lone-linker LL-Sal3 (Ref. Development 127: 1737-1749 (2000) [PMID: 10725249]), purified by phenol/chloroform, and separated from free linkers by Centricon 100.
		The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100.
	lgsun.grc.nia.nih.gov /cDNA/Library3.html (4839 words)

	Characterization of Ripening-Regulated cDNAs and Their Expression in Ethylene-Suppressed Charentais Melon Fruit -- ...
		A, Group 1 cDNAs showed a decrease in gene expression in antisense fruit relative to wild type and were induced in antisense fruit treated with ethylene.
		B, Group 2 cDNA expression was not greatly reduced in antisense fruit relative to wild type, and gene expression was relatively unchanged when antisense fruit were exposed to ethylene.
		Expression of 15 of the 16 cDNAs was shown to be ripening regulated and expression in 12 was fruit specific.
	www.plantphysiol.org /cgi/content/full/122/3/977 (4398 words)

	PG-III: 139 - ANALYSIS OF cDNAs DERIVED FROM THE ARABIDOPSIS YAC CLONE EG10D9 (Site not responding. Last check: 2007-10-22)
		Although the projects on the random-sequencing of cDNAs have progressed successfully in Arabidopsis, a technique is required to identify and map the expressed genes efficiently on Arabidopsis chromosome.
		The hybridized CDNA inserts were then recovered from the DNA-latex beads so that the CDNA library could be prepared.
		The cDNAs derived from the sublibrary are ranged 0.5-2kbp in length.
	www.intl-pag.org /pag/3/abstracts/139pg3.html (267 words)

	Sharma, Mol Vis 2002; 8:251-258.
		Approximately one thousand of the resulting subtracted cDNA clones were partially sequenced and analyzed, and a non-redundant set of one hundred of these cDNAs were examined for tissue expression pattern using a mini-Northern blot procedure and for identity by sequence analysis.
		Although these cDNAs had no homology to "known genes," some bovine cDNAs exhibited significant homology to uncharacterised human cDNAs or to human genomic sequences in the database (Table 3) suggesting that latter are the human orthologues of the bovine clones.
		In conclusion, all the subtracted bovine cDNAs whose expression was detectable by Northern blotting were expressed in the RPE and/or retina, demonstrating the efficacy of the subtraction method.
	www.molvis.org /molvis/v8/a32 (4968 words)

	Full-length cDNAs: more than just reaching the ends -- DAS et al. 6 (2): 57 -- Physiological Genomics
		Following ligation, the cDNA is fractionated on sucrose gradients to eliminate unligated adaptors, dimerized adaptors, and truncated cDNAs.
		Cytoplasmic and nuclear RNA was converted to cDNA using random hexamers as primers and Superscript II as recommended by LTI.
		BstXI adaptors and BstXI dimers from the cDNA mixture.
	physiolgenomics.physiology.org /cgi/content/full/6/2/57 (9953 words)

	DeWitt
		The specific aims of the gene expression project are to (1) elucidate the metal-specificity of putative cadmium-induced cDNAs; (2) detect and sequence putative cadmium-induced cDNAs; and (3) isolate and sequence the corresponding full-length cDNAs for possible identification.
		A student working on this project isolate RNA from cells under conditions of interest, design sequence-specific primers for the putative cDNAs already in hand, and use those primers to drive RT PCR reactions on the isolated RNA to determine whether or not those sequences are expressed under the various conditions.
		A student working on this project would isolate RNA, use the RNA in RT-PCR reactions using degenerate primers or as template for subtractive hybridization, and isolate, amplify and clone promising new target cDNAs for sequencing to be used for future characterization studies.
	www.sfsu.edu /~seo/pages/DeWitt.html (404 words)

	Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery ...
		Highly abundant cDNAs are indicated with an arrow and are removed in the normalized/subtracted cDNAs.
		cDNA twice with 70% ethanol and resuspended it in 50 µL of water.
		Single clones from the full-length cDNA encyclopedia (http://genome.rtc.riken.go.jp/) were rearrayed for the subtraction.
	www.genome.org /cgi/content/full/10/10/1617 (6604 words)

	Addition of non-genomically encoded nucleotides to the 3'-terminus of maize mitochondrial mRNAs: truncated rps12 mRNAs ...
		Maize mitochondrial RNA (1–2 µg) was incubated with 40 pmol of anchor oligonucleotide in the presence or absence of T4 RNA ligase.
		Sequences of the 3'-terminus of the (A) atp9 and (B) cox2 cDNAs.
		(B) cDNA clone C (3'-CCA) (A) was used as probe in a nuclease S1 protection experiment (lane 3) and demonstrates that the cDNA clone protects a few additional nucleotides relative to the genomic clone (lane 2).
	nar.oxfordjournals.org /cgi/content/full/28/22/4444 (4353 words)

	Generation of influenza A viruses entirely from cloned cDNAs -- Neumann et al. 96 (16): 9345 -- Proceedings of the ...
		With both methods, transfectant viruses (i.e., those containing RNA derived from cloned cDNA) are selected from the helper virus population.
		cDNAs derived from influenza virus were amplified by PCR, digested with BsmBI, and cloned into the BsmBI sites of the pHH21 vector (34), which contains the human RNA polymerase I promoter (P) and the mouse RNA polymerase I terminator (T).
		Plasmids containing the RNA polymerase I promoter, a cDNA for each of the eight viral RNA segments, and the RNA polymerase I terminator are transfected into cells together with protein expression plasmids.
	www.pnas.org /cgi/content/full/96/16/9345 (4968 words)

	A method to identify cDNAs based on localization of green fluorescent protein fusion products -- Misawa et al. 97 (7): ...
		After converting the library to retroviruses by using Bosc23-packaging cells, the derived retroviruses were used to infect NIH 3T3 cells, and the clones of interest were subjected for further analysis as described in the experimental protocol.
		The cells exhibiting specific cytoplasmic staining patterns were found to harbor reasonable cDNAs (Table 1).
		The cDNA derived from clone 8 encoded a mouse homologue
	www.pnas.org /cgi/content/full/97/7/3062 (3193 words)

	PAG-X: FULL LENGTH CDNAS OF GRAPEVINE (Site not responding. Last check: 2007-10-22)
		The challenge for our biotech company is to establish the transcriptome of grapevine (Vitis vinifera) by isolating and sequencing the nearly complete set of full-length transcripts.
		We have constructed cDNA libraries from a variety of organs, including berries at different developmental stages.
		About 20,000 clones have been sequenced so far.
	www.intl-pag.org /pag/10/abstracts/PAGX_P116.html (172 words)

	Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs ...
		Every cDNA was aligned with the UniGene data set to identify the number of EST clusters that was hit/joined with a given cDNA.
		The fraction and the total number (in parentheses) of the cDNAs are given for the varying numbers of clusters being hit.
		The cDNA sequence of DKFZp434P211 was taken as reference; identical residues in other sequences are indicated with a dot, residues deviating from the consensus are printed.
	www.genome.org /cgi/content/full/11/3/422 (6726 words)

	Jarvis Lab -- Songbird Neurogenomics
		The RIKEN genomics group is experienced with the generation of full-length and normalized cDNAs from their work with the mouse genome.
		The brain mRNA is isolated, converted to cDNAs, the cDNAs then ligated into a viral vector, followed by in-vitro plasmid excision to form pFLC-I songbird containing clones.
		The cDNA molecules on glass slides are called probes, and the labeled cDNA population used to hybridize them are called targets.
	www.jarvislab.net /Genomics.html (632 words)

	PLANT MUTANTS AND LEAF CDNAS MAPPED IN CONCERT (Site not responding. Last check: 2007-10-22)
		Localize 100 anchor cDNAs from rice on the maize RFLP map.
		The goal of this research is to increase the number of sequenced cDNA markers onthe RFLP map and phenotypic mutants mapped relative to RFLPs to identify potential cDNA/phenotype pairs.
		A set of 150 barley, rice, and oat cDNAs from Cornell and 200 rice probes from the Japanese Rice Genome Project have been screened and probes with strong signal mapped.
	www.nal.usda.gov /pgdic/pggrantinfo/1994/9148880.html (404 words)

	A Streamlined Process to Phenotypically Profile Heterologous cDNAs in Parallel Using Yeast Cell-Based Assays -- ... (Site not responding. Last check: 2007-10-22)
		Heterologous cDNAs from cDNA collections are transferred in parallel to yeast expression vectors and transformed into yeast strains optimized for drug screening.
		As shown in Table 1, 16 cDNAs were cloned in parallel using GRIPP.
		For those cDNAs that we scored as positive for growth interference in yeast, we showed that the phenotype was due to the activity
	www.genome.org /cgi/content/full/11/11/1899 (8800 words)

	Glycosylation and proteolyticprocessing of 70 kDa C-terminal recombinant polypeptides of Plasmodiumfalciparum merozoite ...
		The construction of cDNAs that encode the 70 kDa C-terminal
		GPI anchorsignal; cDNA P3 is devoid of both the signal peptide
		cDNAs that encode the 70-kDaC-terminal portion of P.falciparum MSP-1 with orwithout the MSP-1 signal peptide sequence (Si) at the N-terminalend and with or without the MSP-1 GPI anchor signal sequence (A)at the C-terminal end.
	glycob.oxfordjournals.org /cgi/content/full/9/12/1347 (4662 words)

	BDGP: cDNA & EST Projects (Site not responding. Last check: 2007-10-22)
		A selection of non-redundant clones from over 10,000 full-insert sequenced cDNAs that can be utilized for proteomics and functional genomics
		A description of a new directed approach to isolate missing cDNAs
		cDNA sequence data sets and annotations in fasta or xml formats.
	www.fruitfly.org /EST (123 words)

	Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies -- Haas et al. 31 (19): 5654 ...
		PASA has a parameter, called ‘fuzz distance’, which specifies the length of mismatches to discount at transcript ends, where sequence alignment quality is often poor.
		The cDNA alignments supporting the five splicing variations identified for the WD-40 repeat gene (At2g32700) are illustrated.
		Xiao,Y.L., Malik,M., Whitelaw,C.A. and Town,C.D. (2002) Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2 of Arabidopsis.
	nar.oxfordjournals.org /cgi/content/full/31/19/5654 (7327 words)

	Cloning Antibody cDNAs
		In this series of labs we have been attempting to clone antibody cDNAs using RT-PCR.
		This is smaller than we would predict for a nearly full-length heavy chain cDNA.
		(We expect to amplify a nearly full-length cDNA since the upstream primer is at the translational start site and the downstream primer is in the 3' UTR.) It is this 1,100 bp product that was sent out for sequencing.
	www.csus.edu /indiv/e/ewingn/index/CloningAntibodyseq.html (510 words)

	Prostate Epithelium-derived Ets Transcription Factor mRNA Is Overexpressed in Human Breast Tumors and Is A Candidate ...
		cDNA was used as a template in real-time PCR.
		Table 2 Relative frequencies of specific cDNAs in the cDNA libraries from human normal and tumor tissues
		Amplification was for 25 cycles, and the PCR-amplified PDEF cDNA in the top panel (indicated by an arrow) was 452 nucleotides long.
	clincancerres.aacrjournals.org /cgi/content/full/7/9/2731 (5271 words)

	ARS \| Publication request: Molecular Cloning of Trypsin-Like Cdnas and Comparison of Proteinase Activities in the ... (Site not responding. Last check: 2007-10-22)
		Citation: Zhu, Y., Zeng, F., Cohen, A.C., Oppert, B.S. Molecular Cloning Of Trypsin-Like Cdnas And Comparison Of Proteinase Activities In The Salivary Glands And Gut Of The Tarnished Plant Bug Lygus Lineolaris (Hemiptera: Miridae).
		Three trypsin-like cDNAs, LlSgP2-4, and one trypsin-like cDNA, LlGtP1, were cloned from salivary glands and gut, respectively.
		Deduced trypsin-like proteins from LlSgP2, LlSgP3, and LlGtP1 cDNAs shared 98-99% sequence identity with a previously reported trypsin-like precursor, whereas the trypsin-like protein of LlSgP4 shared only 44% sequence identity with all other trypsin-like proteins, indicating multi-trypsin forms are present in L. lineolaris.
	ars.usda.gov /research/publications/Publications.htm?seq_no_115=146470 (990 words)

	Cytokine Family Database (Site not responding. Last check: 2007-10-22)
		EST sequences in the dbCFC were periodically BLAST-searched by us, and the identification of each EST record in the dbCFC is not necessarily identical to the identifier that is found, for example, in the DEFINITION line of the GenBank record.
		Details of cDNA libraries (tissue types, etc.) are available for the EST records deposited by Washington U-Merck EST Project, and The I.M.A.G.E. Consortium.
		If you have any comments and suggestions, send a mail to dbCFC-admin.
	cytokine.medic.kumamoto-u.ac.jp (216 words)

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