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Topic: COPII

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	Concentrative sorting of secretory cargo proteins into COPII-coated vesicles -- Malkus et al. 159 (6): 915 -- The ...
		10 fold in COPII vesicles relative to phospholipid.
		We mutated this diacidic motif in Gap1p en bloc to alanine
		Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat.
	www.jcb.org /cgi/content/full/159/6/915 (4327 words)

	Self-assembly of minimal COPII cages
		As the pH was decreased to 6.00, the proportion of unsassembled material decreased, and the diameter of the aggregates increased from 70 nm to 110 nm, suggesting flexibility in the self-assembly process (Fig.
		For COPII, lattices cannot be observed easily by conventional EM, either for the protein coat on membranes (Barlowe et al., 1994; Matsuoka et al., 1998) or for the protein aggregates in solution (this study).
		The assembly of the COPII coat on lipid membranes is strictly dependent on the presence of Sar1−GTP at the bilayer surface (Barlowe et al., 1994; Matsuoka et al., 1998).
	www.nature.com /embor/journal/v4/n4/full/embor812.html (3829 words)

	[No title] (Site not responding. Last check: 2007-10-12)
		COPII vesicle bind cargo during the successive recruitment of a small GTP-ase Sar1 followed by protein complexes Sec23/24 and Sec 31/13 from the cytosol.
		In particular we are focussing of mitotic phosphorylation of COPII coat components and its role in recruitment and function of COPII components.
		COPII vesicle formation is restricted as cells enter mitosis but recycling via COPI dependent mechanism depleting the Golgi stack of membrane and leaving Golgi residents in a shrunken remnant, the Golgi cluster.
	www.personal.dundee.ac.uk /~jmlucocq/text.htm (604 words)

	Randy Schekman
		A direct interaction between one of the COPII subunits, Sec24p, and membrane proteins is implicated in the capture of cargo proteins.
		Liposomes formulated with phospholipids representative of a yeast ER membrane fraction bind the COPII proteins in the same sequence of events and with the same nucleotide dependence as observed with native ER membrane.
		Furthermore, COPII buds and vesicles form on the surface of the liposome and capture solute from the interior of the liposome.
	www.hhmi.org /research/investigators/schekman.html (1218 words)

	Sec16p potentiates the action of COPII proteins to bud transport vesicles -- Supek et al. 158 (6): 1029 -- The Journal ...
		by incubation of liposomes with COPII and Sec16p.
		COPII coat subunit interaction: Sec24p and Sec23p bind to adjacent regions of Sec16p.
		COPII coat subunit interactions in the assembly of the vesicle coat.
	www.jcb.org /cgi/content/full/158/6/1029 (6025 words)

	COPII Child Outreach Partnership Initiative, Inc. Art Therapy Summer Camp for Romanian Orphans
		Child Outreach Partnership Initiative, Incorporated or C.O.P.I.I (from the Romanian word “copii” for children) is an art therapies summer camp for abandoned children in Romania.
		We couple expressive therapists from the United States with orphanage teachers and invite groups of high school students to develop a mentoring relationship with the children that will prosper year round.
		C.O.P.I.I. combats the commonly held perception that the children are incapable of inventiveness by educating camp participants in a new way of working with the children and fostering respect for each child’s individuality and creative potential.
	www.copiiproject.org (138 words)

	Dartmouth Medical School: Barlowe Laboratory - Research Projects
		Transport between these compartments is mediated by membrane vesicles, termed COPII vesicles, that bud from the ER and fuse with and/or form the Golgi complex.
		The coat protein complex II (COPII) catalyzes transport vesicle formation from the ER and segregates secretory proteins from ER-resident proteins.
		We hypothesize that Uso1p tethers vesicles by binding to factors on the surface of COPII vesicles and to a Ypt1p protein complex on acceptor membranes.
	dms.dartmouth.edu /barlowe/research (835 words)

	Randy Schekman
		These proteins comprise a novel coat protein complex, COPII, which is distinct from the coat complex, COPI, involved in vesicle budding in the Golgi apparatus.
		A comparable reaction with mammalian COPII proteins has been reconstituted and is being used to explore the mechanism of transport of proteins implicated in familial forms of Alzheimer's disease.
		The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins.
	mcb.berkeley.edu /faculty/CDB/schekmanr.html (721 words)

	Faculty
		At present, it is not clear why some proteins are exported from the ER to the Golgi apparatus by COPII coated vesicles, while others remain behind in the ER.
		COPII machinery in a specific orientation to result in efficient packaging.
		In contrast, the mechanisms responsible for the sorting of soluble cargo molecules into COPII vesicles are unknown.
	www.uic.edu /com/bcmg/steff.html (860 words)

	Surface structure of the COPII-coated vesicle -- Matsuoka et al. 98 (24): 13705 -- Proceedings of the National Academy ...
		The spatial arrangement of COPII coat protein subunits was analyzed by crosslinking to an artificial membrane surface and
		We have analyzed COPII vesicles as a model to understand how transport vesicles are formed during the protein transport process.
		Purified COPII proteins were prepared as described previously (3, 4).
	www.pnas.org /cgi/content/full/98/24/13705 (2943 words)

	TechRef-155 - COPI-COATED ER-TO-GOLGI TRANSPORT COMPLEXES SEGREGATE FROM COPII IN CLOSE PROXIMITY TO ER EXIT SITES.
		Genetic, biochemical and morphological data have accumulated into a model which suggests a sequential mode of action with COPII mediating the selection of cargo and formation of transport vesicles at the ER membrane for ER-to-Golgi transport and COPI mediating recycling of the transport machinery from post-ER membranes.
		To test this transport model directly in vivo, and to study the precise temporal sequence of COPI and COPII action in ER-to-Golgi transport, we have used time lapse microscopy of living cells to visualise simultaneously the dynamics of COPII and COPI, as well as COPII and GFP tagged secretory markers in living cells.
		COPII is not present on these transport complexes and remains associated with the ER.
	www.glass-bottom-dishes.com /technical/technical-155.html (327 words)

	PhD \| Project Details
		COPII is responsible for the export of proteins from the ER.
		While much is known about the function of COPII from yeast genetics and in vitro assays, little is known about the role of COPII in mammalian cells.
		The project will use biochemistry, cell and molecular biology (siRNA transfection, live cell imaging of GFP fusion proteins and immunofluorescence) to characterize compounds that selectively inhibit secretion The aim of the project is to identify the targets of these compounds and to define the molecular basis of their selectivity.
	www.findaphd.com /search/showproject.asp?projectid=6880 (544 words)

	Structure reveals details of cell’s cargo-carriers
		Understanding vesicle "budding" is one of the prerequisites for learning how proteins and other molecules are routed to their correct destinations in the cell.
		The pre-budding complex is the triggering component of a protein coat called COPII that grabs a section of the ER membrane, pinches it off to form the vesicle and packages the protein cargo to be transported.
		"What makes the COPII coat unique is that encoded in its proteins is much of the information that tells it to go to the endoplasmic reticulum and which cargo to take up from the ER," said Goldberg.
	www.eurekalert.org /pub_releases/2002-09/hhmi-srd091802.php (688 words)

	Potassium channels as tools for studying the intracellular vesicular traffic of membrane proteins (Site not responding. Last check: 2007-10-12)
		Transport from the endoplasmic reticulum to the cis-Golgi depends on the COPII coat protein complex.
		The COPII coat consists of the Sar1 GTPase, the Sec23-Sec24p complex and the Sec13-Sec31p complex.
		Budding occurs by the polymerisation of the COPs and is accompanied by the recruitment of SNARE proteins (7-9).
	www.uni-marburg.de /physiology/Daut/Forschung/tools.htm (2530 words)

	Reconstitution of Coat Protein Complex II (COPII) Vesicle Formation from Cargo-reconstituted Proteoliposomes Reveals ...
		COPII vesicles, it is important to examine GTP hydrolysis of
		COPII vesicle fractions 5–8 from a were used as a "vesicle fraction." The amounts of fluorescent lipids in the vesicle fraction is the same as in proteoliposome lane x1.
		Total reactions (5, 3, and 1%), budded COPII vesicles isolated after incubation with (lanes 2 and 3) or without (lane 1) COPII proteins were analyzed by SDS-PAGE followed by immunoblotting.
	www.jbc.org /cgi/content/full/279/2/1330 (3686 words)

	Kinase Signaling Initiates Coat Complex II (COPII) Recruitment and Export from the Mammalian Endoplasmic Reticulum -- ...
		COPII polymerization by interfering with the recruitment of the
		COPII vesicles are believed to fuse to form pre-Golgi intermediates
		COPII recruitment (as analyzed by Sec23 binding); this occurred
	www.jbc.org /cgi/content/full/275/46/35673 (3784 words)

	The Laboratory of Tom Walz
		Transport of proteins in eukaryotic cells from the endoplasmic reticulum (ER) to the Golgi complex proceeds by deformation of specialized portions of the donor membrane to form carrier vesicles.
		A group of cytosolic proteins collectively known as COPII carry out a programmed set of sequential interactions, leading to cargo sorting and vesicle budding.
		It is thought that on GTP hydrolysis, Sar1p-GDP is released, leading to uncoating before fusion of the vesicle to the target membrane and recycling of COPII components.
	walz.med.harvard.edu /Research/Vesicular_Coat_Proteins/COPII.php (655 words)

	Erv14p Directs a Transmembrane Secretory Protein into COPII-coated Transport Vesicles -- Powers and Barlowe 13 (3): 880 ...
		COPII vesicles by using an in vitro budding assay that reconstitutes
		(C) Isolation of COPII complexes from CBY409 (wild-type), CBY808 (91-95A), and CBY809 (97-101) microsomes as in Figure 2 except lanes 2, 6, and 10 are beads alone; lanes 3, 7, and 11 are complete reactions minus GMP-PNP; and lanes 4, 8, and 12 are complete reactions (GMP-PNP, Sec23p/24p complex, and Sar1-GST).
		COPII budding machinery accumulated in the ER and was detected
	www.molbiolcell.org /cgi/content/full/13/3/880 (6948 words)

	COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport ...
		COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle -- Siddiqi et al.
		COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle
		COPII or associated proteins are required for PCTV fusion with
	jcs.biologists.org /cgi/content/full/116/2/415 (7644 words)

	Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat -- ...
		Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat -- Contreras et al.
		Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat
		COPII coat proteins from plant cytosol compete for binding to
	pcp.oxfordjournals.org /cgi/content/abstract/45/12/1779 (248 words)

	Rust, René Christian : Die Vesikel des Poliovirus-Replikationskomplex entstehen an den Membranen des ... (Site not responding. Last check: 2007-10-12)
		The exclusion of ER membrane proteins, that was previously reported for the vesicles of the cellular antero rade transport, was shown to occur with the formation of the Poliovirus vesicles also.
		Furthermore, it was shown that Poliovirus vesicles that accumulated after either the expression of PV protein 2BC or the entire set of the viral non structural proteins, were also carrying the COPII coat proteins and thus were produced by the COPII mechanism like the vesicles during the poliovirus infection.
		These results demonstrate that Poliovirus vesicles are formed at the ER membranes by the cellular COPII mechanism homologous to the anterograde transport vesicles and not through the function of a single virale protein.
	bibd.uni-giessen.de /ghtm/2002/uni/d010158b.htm (470 words)

	COPII - Wikipedia, the free encyclopedia
		COPII vesicles transport proteins from the rough endoplasmic reticulum to the Golgi apparatus.
		The coats of COPII vesicles consist of large protein subcomplexes that consist of four different coat-protein subunits.
		This page was last modified 13:51, 5 February 2006.
	en.wikipedia.org /wiki/COPII (74 words)

	Dartmouth Medical School: Barlowe Laboratory - Publications
		Miller, E.A., Liu, Y. Barlowe, C., Schekman, R. (2005) ER-Golgi transport defects are associated with mutations in the Sed5p-binding domain of the COPII coat subunit, Sec24p.
		Otte, S., Belden, W.J., Heidtman, M., Liu, J., Jensen, O.N. and Barlowe, C. (2001) Erv41p and Erv46p: New components of COPII vesicles involved in transport between the ER and Golgi complex.
		Cao, X. and Barlowe, C. (2000) Asymmetric requirements for a Rab GTPase and SNARE proteins in fusion of COPII vesicles with acceptor membranes.
	dms.dartmouth.edu /barlowe/publications (781 words)

	Cellular COPII Proteins Are Involved in Production of the Vesicles That Form the Poliovirus Replication Complex -- Rust ...
		COPII proteins Sar1, Sec23p and Sec24p, and Sec13 and Sec31 are
		The budding process of vesicles carrying the COPII complex was visualized in PV-infected BT7-H cells.
		COPII complex is functional (3, 76, 77) and acts as the
	jvi.asm.org /cgi/content/full/75/20/9808 (7476 words)

	Schekman papers (Site not responding. Last check: 2007-10-12)
		Miller, E. A., Liu, Y., Barlowe, C., and Schekman, R. ER-Golgi transport defects are associated with mutations in the Sed5p-binding domain of the COPII coat subunit, Sec24p.
		SEC31 encodes an essential component of the COPII coat required for transport vesicle budding from the endoplasmic reticulum.
		GPI-anchor attachment is required for Gas1p transport from the endoplasmic reticulum in COPII vesicles.
	mcb.berkeley.edu /labs/schekman/papers.html (2645 words)

	[No title]
		This is a good overview of the forward pathway employing COPII vesicles and the backward pathway employing COPI vesicles.
		These buds likely form the small COPII vesicles that move secretory proteins from the rough ER to the Golgi complex.
		The small vesicles located near the Golgi stacks are mostly COPI vesicles involved in the backward or retrograde flow of enzymes and proteins that function in younger Golgi cisternae.
	web.uconn.edu /mcb380/lecture10.html (1691 words)

	Clathrin and Two Components of the COPII Complex, Sec23p and Sec24p, Could Be Involved in Endocytosis of the ... (Site not responding. Last check: 2007-10-12)
		Clathrin and Two Components of the COPII Complex, Sec23p and Sec24p, Could Be Involved in Endocytosis of the Saccharomyces cerevisiae Maltose Transporter -- Peñalver et al.
		Clathrin and Two Components of the COPII Complex, Sec23p and Sec24p, Could Be Involved in Endocytosis of the Saccharomyces cerevisiae Maltose Transporter
		Internalization and degradation of the transporter in mutants deficient in COPII components.
	jb.asm.org /cgi/content/full/181/8/2555 (4513 words)

	[No title] (Site not responding. Last check: 2007-10-12)
		Casa de Copii nu e Acasa“ campaign was launched on November 22nd, 2001 in a press conference held by Mr.
		Yet, the most reassuring thing is that there are many calls regarding adoption, which demonstrates the availability to offer a home to some of these children.
		All these things as well as many, many others, that we have nor the time or space to mention here, are the living expression of one desire: to stay close to these children, to change their life for the better, to offer them the love and warmth of a home and a family.
	www.copii.ro /home.doc (1676 words)

	COPII Coat Assembly and Selective Export from the Endoplasmic Reticulum -- Sato 136 (6): 755 -- Journal of Biochemistry
		COPII Coat Assembly and Selective Export from the Endoplasmic Reticulum -- Sato 136 (6): 755 -- Journal of Biochemistry
		COPII Coat Assembly and Selective Export from the Endoplasmic Reticulum
		that the COPII coat is responsible for direct capture of membrane
	jb.oupjournals.org /cgi/content/abstract/136/6/755 (175 words)

	HHMI News: Structure Reveals Details of Cell’s Cargo-Carriers
		Jonathan Goldberg, Xiping Bi and Richard Corpina at Memorial Sloan-Kettering Cancer Center unveil the intricate architecture of the “pre-budding complex,” which is a set of proteins that participates in the formation of vesicles on the cell’s endoplasmic reticulum (ER).
		Randy Schekman, a pioneer in vesicle studies at the University of California, Berkeley.
		In order to understand the process of vesicle formation and transport in molecular terms, one must begin with the initiating event — with the multi-component pre-budding complex, Goldberg said.
	www.hhmi.org /news/goldbergj2.html (845 words)

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