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Topic: Capillary electrophoresis

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DNA electrophoresis

HPLC

Gel electrophoresis

Agarose gel electrophoresis

Voltage

Atomic spectroscopy

Protein electrophoresis

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	The Scientist : Capillary Electrophoresis: Automating A Valuable Technique
		The history of capillary electrophoresis is tightly interwoven with that of gel electrophoresis.
		Since the capillaries are so small (usually 10 to 100 centimeters in length, with an inner diameter of 50 to 100 microns), the Joule heat generated by the electric current is quickly dissipated.
		Capillaries are coated with a polymer to minimize sample adsorption and electroendosmosis.
	www.the-scientist.com /article/display/10296 (2145 words)

	Capillary electrophoresis - Wikipedia, the free encyclopedia
		Introduced in the 1960s, the technique of capillary electrophoresis (CE) was designed to separate species based on their size to charge ratio in the interior of a small capillary filled with an electrolyte.
		In general, capillaries used in capillary electrophoresis are coated with a polymer for increased stability.
		The separation of compounds by capillary electrophoresis is dependent on the differential migration of analytes in an applied electric field.
	en.wikipedia.org /wiki/Capillary_electrophoresis (2132 words)

	Capillary Electrophoresis: A Simple Technique
		Capillary electrophoresis (CE) encompasses a family of related separation techniques that use narrow-bore fused-silica capillaries to separate a complex array of large and small molecules.
		Capillary Isoelectric Focusing (CIEF) allows amphoteric molecules, such as proteins, to be separated by electrophoresis in a pH gradient generated between the cathode and anode.
		Capillary Electrochromatography (CEC) is a hybrid separation method that couples the high separation efficiency of CZE with HPLC and uses an electric field rather than hydraulic pressure to propel the mobile phase through a packed bed.
	www.beckman.com /resourcecenter/labresources/ce/cedefinitionmodes.asp (2669 words)

	Capillary Electrophoresis (Site not responding. Last check: 2007-09-18)
		Performing electrophoresis in small-diameter capillaries allows the use of very high electric fields because the small capillaries efficientlydissipate the heat that is produced.
		Capillaries are typically of 50 µm inner diameter and 0.5 to 1 m in length.
		A small volume of sample (10 nL) is injected at the positive end of the capillary and the separated components are detected near the negative end of the capillary.
	elchem.kaist.ac.kr /vt/chem-ed/sep/electrop/cap-el.htm (223 words)

	High speed, high resolution compositions, methods, and kits for capillary electrophoresis - Patent 6706162
		The term "capillary" as used herein, refers to a tube or channel or other structure for carrying out electrophoresis that is capable of supporting a volume of separation medium, such as a composition for separating analytes, as disclosed herein.
		The term "uncoated capillary" means that the capillary is uncoated prior to the introduction of compositions of the invention, i.e., not covalently coated prior to use.
		Capillary electrophoresis elements were prepared by pumping one of the five compositions through uncoated capillaries for 400 seconds before each analysis.
	www.freepatentsonline.com /6706162.html (6425 words)

	Capillary electrophoresis. - Physiology - What's Been Published (Site not responding. Last check: 2007-09-18)
		Capillary electrophoresis : theory & practice / edited by Paul D. Grossman, Joel C. Colburn.
		Capillary electrophoresis : theory and practice / edited by Patrick Camilleri.
		Capillary electrophoresis of nucleic acids / edited by Keith R. Mitchelson and Jing Cheng.
	www.pitbossannie.com /rps-qp-capillary-electrophoresis.html (252 words)

	Intro to Capillary Electrophoresis (Site not responding. Last check: 2007-09-18)
		Electrophoresis is a separation technique based on the differential migration of molecules based on charge when subjected to an electric field.
		Capillary electrophoresis is very efficient at separating hundreds of components simultaneously despite requiring only simple instrumentation and being relatively inexpensive to run.
		Capillary electrophoresis is thus a useful tool in biochemical analysis.
	www.unc.edu /~sidray/CE-intro2.html (129 words)

	DETECTION OF DIVALENT TRANSITION METAL IONS IN COMPLEX MEDIA BY CAPILLARY ELECTROPHORESIS
		Capillary electrophoresis methods have a number of advantages over many of the other methods: ability to quantitate multiple ions in a single sample, sample size, speed, and ease of use.
		Capillary electrophoresis is especially well-suited for analyzing samples generated in screening studies, due to its facility with multiple, small samples.
		But a capillary electrophoresis method suitable for transition metals in water was unsuitable for use in complex media.
	www.engg.ksu.edu /HSRC/97Proceed/Analytical2/detection.html (1693 words)

	Biopharmaceutical chromatography & electrophoresis glossary
		Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis.
		Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
		Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
	www.genomicglossaries.com /content/chromatography_glossary.asp (2004 words)

	SPF: Capillary Electrophoresis (Site not responding. Last check: 2007-09-18)
		The PAN Biotechnology Facility can supply analytical support of capillary electrophoresis by means of Beckman 2000 instrumentation.
		Capillary electrophoresis analyses are available for characterization of synthetic peptides and oligonucleotides and as verification of post synthesis purification procedures.
		To place an order for Capillary Electrophoresis, please print and fill out an order form and mail it to the address provided on the form.
	cmgm.stanford.edu /panfac/ce/index.html (267 words)

	Polymicro Technologies > Catalog > Capillary Electrophoresis
		“Capillary electrophoresis (CE) was born of the marriage of the powerful separation mechanisms of electrophoresis with the instrumentation and automation concepts of chromatography.”
		CE is a separation method based on the differential migration rates of sample components within a capillary when an electrical field is applied axially to that capillary.
		Capillary can be easily cleaved to length, providing users great latitude in adjusting column length and economies of scale in tubing procurement.
	www.polymicro.com /catalog/3_4.htm (623 words)

	Rapid and Automated Genetic Typing using Capillary Electrophoresis for the Analysis of STRs and D1S80
		The recent development of capillary electrophoresis (CE) for DNA typing provides an avenue for this process (2-7).
		A Beckman P/ACETM 2050 capillary electrophoresis instrument with a Laser Module 488 argon ion laser (Beckman Instruments, Fullerton, CA) was used with a 520 nm bandpass filter.
		McCord B.R., McClure D.M. and Jung J.M. (1993) Capillary electrophoresis of PCR-amplified DNA using fluorescence detection with an intercalating dye.
	www.promega.com /geneticidproc/ussymp6proc/mccord.htm (2652 words)

	Capillary Electrophoresis
		Electrophoresis is a powerful method that was developed long before molecular biology came into existence.
		The two limiting factors of traditional electrophoresis were: 1) detection of molecules upon completion of electrophoretic separation and 2) only low voltages could be used to prevent heat damage of the samples.
		Samples are applied to the capillary tubes when the cathode buffer is moved aside and sample chamber placed at the opening of the capillary tube.
	www.bio.davidson.edu /courses/genomics/method/Capillary.html (370 words)

	Modular system for Electrophoresis
		The 2-D capillary gel and electroblotting modules can be purchased as stand-alone units with their own gel tank and lid or as individual modules for use in a previously purchased CDC gel tank.
		The Scie-Plas Capillary Gel Module which performs the first phase of 2-D electrophoresis (Isoelectric Focusing - IEF), allows 10 capillary gels to be run at any one time, using either the 10 x 10cm or 20 x 20cm CDC systems.
		The Scie-Plas capillary tubes have a wide capillary bore diameter, which greatly eases the extraction of the capillary gel once the IEF phase is completed.
	www.topac.com /epv_modular.html (612 words)

	Baker, Dale R.: Capillary Electrophoresis (Site not responding. Last check: 2007-09-18)
		Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is fast, sensitive, easy to automate, requires only small sample volumes and reagent amounts, and has been successfully applied to an ever-expanding list of sample types.
		In Capillary Electrophoresis, analytical practitioners will find a complete, practical guide to the principles, forms, and instrumentation of this technique.
		Capillary Electrophoresis will serve both as an excellent introduction to those who are new to the technique and as a comprehensive reference book to experienced practitioners.
	www.forbesbookclub.com /bookpage.asp?prod_cd=I34MT (196 words)

	Project details (Site not responding. Last check: 2007-09-18)
		The aim of the project is to improve and enlarge the applicability of capillary electrophoresis especially its modification EMMA (Electrophoretically Mediated MicroAnalysis) method for the assay and study of membrane bound enzymes.
		the part of capillary is filled with the buffer suitable for enzyme reaction whereas the rest of the capillary is filled with the background electrolyte optimal for separation of substrate(s) and product(s).
		Various modes of capillary electrophoresis – capillary zone electrophoresis, micellar electrokinetic capillary chromatography, even chiral capillary electrophoresis will be used depending on the nature of studied compounds and their metabolites.
	www.muni.cz /sci/research/projects/2238 (315 words)

	EPRI: Field Tests of Capillary Electrophoresis for Online Sub-ppb Anion Analysis (Site not responding. Last check: 2007-09-18)
		Capillary electrophoresis (CE) is a well-known analytical technique that has potential for quantitative measurement of high mobility anions and cations at very low concentrations.
		The project team optimized the conventional capillary electrophoresis method for extremely low detection limits through several improvements in materials and techniques.
		A previous EPRI report, "Capillary Electrophoresis for On-line Sub-ppb Analysis, Interim Report — Laboratory Evaluation" (1002887), described the development of the high-sensitivity improvements incorporated into a prototype device.
	www.epri.com /OrderableitemDesc.asp?product_id=000000000001007874&targetnid=273227&value=06T061146&marketnid=273141&oitype=1&searchdate=12/3/2003 (567 words)

	Capillary Electrophoresis Systems: Smoothing The Pathway To Discovery - Biocompare Technology Spotlight
		Capillary electrophoresis is providing biologists relief from their universal struggle to separate components of the cell.
		Capillary electrophoresis brought separation into thin capillaries that offer high-resolution of a wide variety of items, including ions, small molecules, macromolecules, viral components, and entire cells.
		In a process that takes less than 30 minutes, an electric voltage applied across the capillary teases apart the sample's components based on their size, charge, hydrophobicity, binding properties, and chirality, depending upon the experimental set-up.
	www.biocompare.com /spotlight.asp?id=250 (506 words)

	Improved Protocol for T-RFLP by Capillary Electrophoresis (Site not responding. Last check: 2007-09-18)
		However, capillary electrophoresis has been gaining popularity, but the standard procedures for the capillary equipment are not suitable for T-RFLP.
		In capillary electrophoresis the injection of DNA samples can be achieved by two methods.
		A fifth to a third of the desalted restriction digest is generally loaded onto the capillary electrophoresis system using the default settings optimized for sequencing.
	rdp8.cme.msu.edu /html/t-rflp_jul02.html (1290 words)

	Capillary Electrophoresis
		The past ten years have seen countless demonstrations of the potential of capillary electrophoresis (CE) in many areas of biotechnology.
		The fundamental difference between traditional slab format electrophoresis and CE is that the separation is achieved inside small fused-silica capillaries, typically 25-100 Âµm, as first demonstrated by Jorgenson and co-workers.
		The most common is capillary zone electrophoresis (CZE), which is similar to traditional paper electrophoresis where charged species migrate in a continuous electrolyte based on their net charge and size.
	www.noble.org /Plantbio/MS/meta.capelect.html (211 words)

	Genotypes in Polyclonal Infections by Single Species \| CDC EID
		Capillary electrophoresis defines the number of genotypes within each allotype and the intensity of infection by genotype.
		The advantages of real-time PCR followed by capillary electrophoresis are that it can be performed without waiting days or weeks for cultures to grow and that it detects pathogens that do not grow in conventional culture media or under standard conditions (31).
		Third, capillary electrophoresis may need to be performed separately for each allotype to avoid confusion between amplicons of similar size from different allotypes (Figures 1–3).
	www.cdc.gov /Ncidod/eid/vol12no03/05-0572.htm (4339 words)

	Electrophoresis - Wikipedia, the free encyclopedia
		Electrophoresis is the movement of an electrically charged substance under the influence of an electric field.
		This movement is due to the Lorentz force, which may be related to fundamental electrical properties of the body under study and the ambient electrical conditions by the equation given below.
		Gel electrophoresis is an application of electrophoresis in molecular biology.
	en.wikipedia.org /wiki/Electrophoresis (502 words)

	Capillary electrophoresis - Lumex International Products
		Based on the differential migration of componenys of aqueous samples within a narrow quartz capillary under the influence of the applied electric field.
		Separated solutes are quantitatively detected at the capillary outlet by high sensitive optical system based on direct or indirect UV absorbance.
		CAPEL-103R/103RT, their upgraded automated version CAPEL-104/104T, and CAPEL-105 are high-performance capillary electrophoresis instruments, equipped with a photometric detector.
	www.lumexint.com /product/kapel104.shtml (595 words)

	index
		As separation in capillary electrophoresis is based on differences in the charge to mass ratio of solvated ions, the technique is an ideal complement to adsorption based chromatographic techniques.
		We are investigating the use of in-line extraction coupled to capillary electrophoresis as a method for detection of trace quantities of these drugs in urine.
		Additionally we are investigating the use of monolithic capillary electrochromatography and the use of CE with fluorescent detection in forensic toxicology.
	www.fiu.edu /~mccordb (1446 words)

	Capillary electrophoresis equipment
		In the process of electrophoresis, proteins are separated and characterized according to attributes like size and charge when moved across an applied electric field.
		The processes are very similar; however, in capillary electrophoresis, the medium used for separation is not a gel but a liquid buffer.
		Sebia, the global leader in electrophoresis innovation, has capitalized on all of these advantages with our latest piece of capillary electrophoresis equipment, the CAPILLARYS™ 2.
	www.sebia-usa.com /siteMap/capillary.html (270 words)

	CAMIS
		Capillary electrophoresis provides first principle insight into the solution charge of macromolecules.
		High field strengths are used to separate proteins introduced into the capillary by either electrokinetic or hydrodynamic means.
		Coated capillaries are used for more basic proteins to prevent adsorption on the capillary wall.
	www.camis.unh.edu /CE/capillary_electrophoresis.html (276 words)

	Capillary Electrophoresis Course 2004 (Site not responding. Last check: 2007-09-18)
		The term Capillary Electrophoresis (CE) covers several modes of separation, all of which harness electrical forces in capillary tubes for analytical purposes.
		CE is used throughout chemistry, pharmaceuticals and biotechnology, with separations of both charged and uncharged species ranging in size from metal ions to proteins and polynucleotides.
		As numbers are limited on the Capillary Electrophoresis Course, early registration is advised.
	www.york.ac.uk /depts/chem/extern/extcourses/cecour04.htm (910 words)

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