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Topic: Caulobacter


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  Caulobacter - MicrobeWiki   (Site not responding. Last check: 2007-10-05)
Caulobacter crescentus is a single-celled, small bacterium that divides asymmetrically producing cells that differ in structure and function - it is an ideal model system for the mechanisms of asymmetric cell division and has been studied thoroughly.
The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,016,942 bp encoding for 3,767 genes.
The cell cycle of Caulobacter showing the two daughter cells, one with a stalk and one with a flagellum, that are different in function and structure.
microbewiki.kenyon.edu /mediawiki-1.6.6/index.php/Caulobacter   (1047 words)

  
 Caulobacter crescentus - Wikipedia, the free encyclopedia
Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams.
Caulobacter is an important model for study of the regulation of the cell cycle and cellular differentiation.
Often surviving in nutrient-poor environs, Caulobacter crescentus is a Gram-negative bacterium ubiquitous in fresh water, soil, and sea water.
en.wikipedia.org /wiki/Caulobacter_crescentus   (277 words)

  
 PSU BMB
We have found that tmRNA is required for regulation of the cell cycle and development in Caulobacter crescentus, and we are using biochemical and genetic approaches to understand the molecular events which underlie the tmRNA mechanism and to understand the role of tmRNA in bacterial differentiation and physiology.
Caulobacter is an ideal organism to study bacterial differentiation and cell-cycle regulation, since each round of the cell cycle includes an obligatory differentiation event (Fig.
In Caulobacter, a deletion of tmRNA results in a delay in the initiation of DNA replication during the swarmer-to-stalked cell transition.
www.bmb.psu.edu /faculty/keiler/keiler.html   (1086 words)

  
 Nexus Research Group - Caulobacter in New Zealand
Caulobacter is one of the many genera (Gram negative and Gram positive) that elaborate a paracrystalline array surface (S) layer on their outermost surface.
Caulobacter could be detected from virtually every type of municipal waste water treatment plant from across the USA and Canada at all points in the process except for the strongly anaerobic regions of sludge digesters used by many facilities to reduce sludge volume and generate methane gas.
Caulobacter were present in the enrichment but not in sufficient numbers for it to be successfully isolated, nor to conclude that they were the mass contaminant.
www.nexusresearchgroup.com /microbiology/caul1994.htm   (3840 words)

  
 FAS Center: Michael Laub
Although many of the major cell-cycle regulators in Caulobacter are known, it remains a major challenge to identify the complete network underlying cell-cycle oscillations and cellular asymmetry.
Caulobacter is a powerful model for studying regulation, as cells are easily synchronized, cell-cycle progression can be tracked by monitoring a series of morphological transitions, and a complete suite of genetic tools is available.
The Caulobacter genome encodes 64 histidine kinases and 42 response regulators, at least 10 of which have been found to be involved in cell-cycle progression, in previous genetic screens.
sysbio.harvard.edu /csb/research/laub.html   (324 words)

  
 Regulation of Stalk Elongation by Phosphate in Caulobacter crescentus -- Gonin et al. 182 (2): 337 -- The Journal of ...   (Site not responding. Last check: 2007-10-05)
by the Pho regulon, the Caulobacter phoB gene that encodes the
Caulobacter cells are typically found in aquatic ecosystems, where the most common limiting nutrient is phosphorus (29).
Expression in Caulobacter crescentus of the phosphate-starvation-inducible porin OprP of Pseudomonas aeruginosa.
jb.asm.org.cob-web.org:8888 /cgi/content/full/182/2/337   (6308 words)

  
 [No title]
N-acetylglucosamine-6-phosphate deacetylase [Caulobacter crescentus CB15] nlqlbt5vvlum3dff CERT 0.00000000200000000000 1moq00 c.80.1.1 >gi16124699refNP_419263.1
N-acetylglucosamine-6-phosphate deacetylase [Caulobacter crescentus CB15] crdoidigkt44n75c CERT 0.00000000100000000000 1moq00 c.80.1.1 >gi16124790refNP_419354.1
TonB-dependent receptor [Caulobacter crescentus CB15] ctqb28s16vln4m4l CERT 0.00000000200000000000 1bhe00 b.80.1.3 >gi16124826refNP_419390.1
bioinf.cs.ucl.ac.uk:8000 /servlet/GTD.GTDGetListServlet?organism=Caulobacter_crescentus   (12040 words)

  
 Researchers pop bacterial hood, uncover engine that drives cell division   (Site not responding. Last check: 2007-10-05)
Caulobacter has even been found living in aquifers contaminated by oil or gasoline, having adapted to consume oil as food.
Caulobacter, on the other hand, always divides into two different daughter cell types, a stalked cell and a motile swarmer cell.
Biologists use Caulobacter as a model to study the molecular processes by which a cell divides into two unequal daughter cells, a fundamental problem in developmental biology.
news-service.stanford.edu /news/medical/2004/may19/bacteria.html   (815 words)

  
 Princeton Caulobacter Laboratory   (Site not responding. Last check: 2007-10-05)
We have now used transcription systems containing highly purified sy from Caulobacter and a s54 holoenzyme from E. coli to demonstrate that both the positive and negative regulation of these genes requires FlbD, a cell cycle regulated ftr sequence-specific DNA binding protein (Benson et al., 1994).
Regulation of the Caulobacter crescentus rpoN gene and function of the purified sigma 54 in flagellar gene transcription.
The cell cycle-regulated flagellar gene flbF of Caulobacter crescentus is homologous to a virulence locus (lcrD) of Yersinia pestis.
www.molbio.princeton.edu /labs/newton/austinframe.html   (1094 words)

  
 Caulobacter Henrici and Johnson 1935, genus
The genus Caulobacter is polyphyletic and only one of the lineages of Caulobacter species can retain the generic name.
Caulobacter crescentus could not be discerned clearly from Caulobacter vibrioides by a polyphasic approach
The type strain of Caulobacter vibrioides Henrici and Johnson 1935 (Approved Lists 1980), which the "Approved Lists of Bacterial Names" list as being "not extant" is in fact held in the DSMZ (strain DSM 9893) and VKM (strain VKM B-1496).
www.bacterio.cict.fr /c/caulobacter.html   (1547 words)

  
 Barnard News Center
Caulobacter divides asymmetrically, with one half of the original cell developing a flagellum, a wavy "tail" attached to the cell, and the second half developing a straight "tail," or a stalk (caulis is Latin for "stalk").
As the two cells develop, the stalk cell renews the division process immediately, while the cell with the flagellum goes through a second stage of developing a stalk before dividing again.
The Caulobacter Meeting, along with others like it, was designed as a specialized breakout meeting for those whose research focuses on that particular bacterium.
www.barnard.edu /newnews/news073102.html   (274 words)

  
 IU Biology Faculty: Yves Brun
We study the simple differentiating bacterium Caulobacter crescentusin which each cell division produces a motile swarmer cell and a stalked cell that attaches to surfaces via its adhesive holdfast.
The recent completion of the genomic sequence of the Caulobacter genome provides new avenues to study the control of cell differentiation.
The Caulobacter crescentus polar organelle development protein PodJ is differentially localized and is required for polar targeting of the PleC development regulator.
www.bio.indiana.edu /facultyresearch/faculty/Brun.html   (701 words)

  
 Nexus Research Group - Isolation of Caulobacter sp.
Caulobacter was first isolated in New Zealand in the Manawatu (1).
Caulobacter may serve as a reservoir of antibiotic resistance determinants which then persist in the environment and can be transferred back to human associated bacteria.
The ability of Caulobacter to survive in oligotrophic conditions is the basis for the enrichment step.
www.nexusresearchgroup.com /research_papers/nzms1999a.htm   (484 words)

  
 Proteomics Database of Caulobacter crescentus, Experimental Procedures
A global approach was used to analyze protein synthesis and stability during the cell cycle of the bacterium Caulobacter crescentus.
The concentration of several important modulators of the Caulobacter cell cycle progression such as the transcriptional regulator CtrA, the cell division protein FtsZ, and the DNA methyltransferase CcrM fluctuate during the cell cycle as a consequence of timed synthesis and degradation.
This is, for instance, the case for the flagellar proteins, which are expressed towards the end of the Caulobacter cell cycle in a three-tired expression cascade resulting in the ordered assembly of a single flagellum at one pole of the predivisional cell.
proteom.biomed.cas.cz /caulob/experimental.php   (9546 words)

  
 Department of Molecular Biology   (Site not responding. Last check: 2007-10-05)
The model system for these studies is the alpha-proteobacterium Caulobacter crescentus, in which the stalked cell divides repeatedly, much like a stem cell, to produce the mother stalked cell plus a new, motile swarmer cell (Figure 1).
Construction of the swarmer cell requires the biosynthesis of a unique set of surface structures, including the flagellum, DNA phage receptors and pili, which are assembled sequentially at one pole of the predivisional cell during the course of the cell cycle (Figure 2 and reviewed in Newton and Ohta 2003).
Thus, the importance of spatial regulation with respect to cell cycle progression and development in Caulobacter is variable.
www.molbio.princeton.edu /research_facultymember.php?id=14   (1703 words)

  
 tmRNA in Caulobacter crescentus Is Cell Cycle Regulated by Temporally Controlled Transcription and RNA Degradation -- ...   (Site not responding. Last check: 2007-10-05)
that the Caulobacter SsrA RNA is transcribed and nucleolytically
Caulobacter strains were grown in M2G or PYE medium at
Genetic analysis of a temporally transcribed chemotaxis gene cluster in Caulobacter crescentus.
jb.asm.org /cgi/content/full/185/6/1825   (3549 words)

  
 Stanford scientists to probe inner workings of remarkable microbe: 11/01
As concern mounts over the spread of anthrax and other types of germ warfare, it is worth bearing in mind that many microbes have the potential to benefit humanity.
Scientists have long been fascinated with the ability of Caulobacter bacteria to divide asymmetrically into two distinct daughter cells -- a "swarmer" equipped with a tiny tail for swimming, and a larger "stalk" cell shaped like a lollipop on a stick.
The life cycle of a Caulobacter swarmer cell is shown through a series of electron micrographs.
news-service.stanford.edu /news/november14/bacteriagrant-1114.html   (1035 words)

  
 New members of the ctrA regulon: the major chemotaxis operon in Caulobacter is CtrA dependent -- Jones et al. 147 (4): ...
Anderson, P. and Gober, J. FlbT, the post-transcriptional regulator of flagellin synthesis in Caulobacter crescentus, interacts with the 5' untranslated region of flagellin mRNA.
Ramakrishnan, G., Zhao, J. and Newton, A. The cell cycle-regulated flagellar gene flbF of Caulobacter crescentus is homologous to a virulence locus (lcrD) of Yersinia pestis.
Yu, J. and Shapiro, L. Early Caulobacter crescentus genes fliL and fliM are required for flagellar gene expression and normal cell division.
mic.sgmjournals.org /cgi/content/full/147/4/949   (4085 words)

  
 Isolation and Characterization of NaCl-Sensitive Mutants of Caulobacter crescentus -- Zuleta et al. 69 (6): 3029 -- ...   (Site not responding. Last check: 2007-10-05)
Isolation and Characterization of NaCl-Sensitive Mutants of Caulobacter crescentus -- Zuleta et al.
Isolation and Characterization of NaCl-Sensitive Mutants of Caulobacter crescentus
Caulobacter crescentus is one of the major models for studying
aem.asm.org /cgi/content/full/69/6/3029   (3896 words)

  
 Nature's strongest glue could be used as a medical adhesive (via CobWeb/3.1 planetlab-3.cs.princeton.edu)   (Site not responding. Last check: 2007-10-05)
The scientists found they had to apply a force of about 1 micronewton to remove a single Caulobacter crescentus from a glass pipette.
By contrast, commercial "super" glue breaks when a shear force of 18 to 28 newtons per square millimeter is applied.
Caulobacter crescentus affixes itself to solid objects with its stalk and holdfast.
newsinfo.iu.edu.cob-web.org:8888 /news/page/normal/3258.html   (530 words)

  
 Why Sequence Two Caulobacter Species?
As ubiquitous inhabitants of aquatic ecosystems, particularly nutrient-poor ("oligotrophic") habitats, Caulobacters may be exploitable for bioremediation applications.
The polar stalk is a key appendage in the Caulobacter life cycle, in which the stalked cell acts as a stem cell to generate a motile "swarmer" cell.
This fascinating, obligatory cell cycle has made Caulobacter crescentus one of the dominant prokaryotic model systems for studying molecular mechanisms of cell-cycle control and cellular differentiation.
www.jgi.doe.gov /sequencing/why/CSP2006/Caulobacters.html   (309 words)

  
 The phylogenetic relationships of Caulobacter, Asticcacaulis and Brevundimonas species and their taxonomic implications ...
The phylogenetic relationships of Caulobacter, Asticcacaulis and Brevundimonas species and their taxonomic implications -- Sly et al.
The phylogenetic relationships of Caulobacter, Asticcacaulis and Brevundimonas species and their taxonomic implications
Caulobacter reported previously [Stahl, D. A., Key, R., Flesher, B. and
ijs.sgmjournals.org /cgi/content/abstract/49/2/483   (391 words)

  
 Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: molecular characterization of the polyhydroxybutyrate ...
Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: molecular characterization of the polyhydroxybutyrate synthase -- Qi and Rehm 147 (12): 3353 -- Microbiology
Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: molecular characterization of the polyhydroxybutyrate synthase
Caulobacter crescentus was investigated with respect to polyhydroxybutyrate
mic.sgmjournals.org /cgi/content/full/147/12/3353   (2531 words)

  
 UIC - DDN - Dr. Malakooti
A consensus promoter sequence for Caulobacter crescentus genes involved in biosynthetic and housekeeping functions.
Identification and characterization of the ilvR gene, a LysR-type regulator of the Caulobacter crescentus.
Molecular characterization, nucleotide sequence and expression of four flagellar genes, fliO, fliP, fliQ, and fliR of Escherichia coli: FliP is a membrane protein with N-terminal signal sequence.
www.uic.edu /com/dom/gastro/malakooti.html   (545 words)

  
 Caulobacter crescentus Requires RodA and MreB for Stalk Synthesis and Prevention of Ectopic Pole Formation -- Wagner et ...   (Site not responding. Last check: 2007-10-05)
Caulobacter crescentus Requires RodA and MreB for Stalk Synthesis and Prevention of Ectopic Pole Formation -- Wagner et al.
Proteomic analysis of the Caulobacter crescentus stalk indicates competence for nutrient uptake.
Use of the Caulobacter crescentus genome sequence to develop a method for systematic genetic mapping.
jb.asm.org.cob-web.org:8888 /cgi/content/full/187/2/544   (5969 words)

  
 Oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate ...
Anast, N. and Smit, J. Isolation and characterization of marine caulobacters and assessment of their potential for genetic experimentation.
Merker, R. and Smit, J. Characterization of the adhesive holdfast of marine and freshwater caulobacters.
Nikitin, D. I., Vishnewetskaya, O. Yu., Chumakov, K. and Zlatkin, I. Evolutionary relationship of some stalked and budding bacteria (genera Caulobacter, "Hyphobacter", Hyphomonas and Hyphomicrobium) as studied by the new integral taxonomical method.
ijs.sgmjournals.org /cgi/content/full/53/6/1901   (2707 words)

  
 Cell surface patterning and morphogenesis: biogenesis of a periodic surface array during Caulobacter development -- ...   (Site not responding. Last check: 2007-10-05)
Cell surface patterning and morphogenesis: biogenesis of a periodic surface array during Caulobacter development -- Smit and Agabian 95 (1): 41 -- The Journal of Cell Biology
Cell surface patterning and morphogenesis: biogenesis of a periodic surface array during Caulobacter development
Caulobacter crescentus Synthesizes an S-Layer-Editing Metalloprotease Possessing a Domain Sharing Sequence Similarity with Its Paracrystalline S-Layer Protein
www.jcb.org /cgi/content/abstract/95/1/41   (479 words)

  
 An SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus -- Galhardo et al. 33 (8): ...   (Site not responding. Last check: 2007-10-05)
An SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus -- Galhardo et al.
Bender, R.A. (1984) Ultraviolet mutagenesis and inducible DNA repair in Caulobacter crescentus Mol.
Gober, J.W. and Shapiro, L. (1992) A developmentally regulated Caulobacter flagellar promoter is activated by 3' enhancer and IHF binding elements Mol.
nar.oxfordjournals.org /cgi/content/full/33/8/2603   (6248 words)

  
 Defining Absolute Confidence Limits in the Identification of Caulobacter Proteins by Peptide Mass Mapping   (Site not responding. Last check: 2007-10-05)
This paper presents a systematic study of the impact of guanidination on proteomic analysis of an entire bacterial organelle.
Sixty-two 2-D gel isolated proteins from Caulobacter crescentus stalks were studied.
The advantages of guanidination are illustrated using both experimental and theoretical data.
pubs.acs.org /cgi-bin/abstract.cgi/jprobs/2002/1/i04/abs/pr025518b.html   (167 words)

  
 Publication list Prof. Urs jenal   (Site not responding. Last check: 2007-10-05)
Protease susceptibility of the Caulobacter crescentus flagellar hook-basal body: a possible mechanism of flagellar ejection during cell differentiation.
Fuchs, T., Wiget, P., Osteras, M., and Jenal, U. (2001) Precise amounts of a novel member of a phosphotransferase superfamily are essential for growth and normal morphology in Caulobacter crescentus.
EMBO J. Mohr, C.D., Jenal, U., and Shapiro, L. (1996) Flagellar assembly in Caulobacter crescentus: a basal body P-ring null mutation affects stability of the L-ring protein.
www.biozentrum.unibas.ch /jenal/jenal_pub.html   (840 words)

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