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Topic: Caulobacter crescentus


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In the News (Thu 17 Dec 09)

  
  Caulobacter - MicrobeWiki
Caulobacter crescentus is a single-celled, small bacterium that divides asymmetrically producing cells that differ in structure and function - it is an ideal model system for the mechanisms of asymmetric cell division and has been studied thoroughly.
The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,016,942 bp encoding for 3,767 genes.
The cell cycle of Caulobacter showing the two daughter cells, one with a stalk and one with a flagellum, that are different in function and structure.
microbewiki.kenyon.edu /index.php/Caulobacter   (1047 words)

  
 Caulobacter
Caulobacter crescentus could not be discerned clearly from Caulobacter vibrioides by a polyphasic approach
Caulobacter maris Poindexter 1964 (Approved Lists 1980) has been tranferred in the genus ¤ Maricaulis Abraham et al.
The type strain of Caulobacter vibrioides Henrici and Johnson 1935 (Approved Lists 1980), which the "Approved Lists of Bacterial Names" list as being "not extant" is in fact held in the DSMZ (strain DSM 9893) and VKM (strain VKM B-1496).
www.bacterio.cict.fr /c/caulobacter.html   (1558 words)

  
 Caulobacter crescentus - Biocrawler   (Site not responding. Last check: 2007-10-20)
Caulobacter crescentus is a gram-negative, oligotrophic bacterium widely distributed in aquatic environments.
The mobile one has a flagellum and swims until it finds a favorable environment, at which point it loses its flagellum and develops a new structure that lets the cell fix itself to a substrate.
The optimal temperature to the growth of Caulobacter is around 30°C and its generational period is 2.5 hours.
www.biocrawler.com /encyclopedia/Caulobacter_crescentus   (133 words)

  
  Genome sequence of the bacterium Caulobacter crescentus   (Site not responding. Last check: 2007-10-20)
crescentus is a simple and highly manipulable single-celled model system to study cellular differentiation, asymmetric division, and their coordination with cell cycle progression," the researchers write in Proceedings of the National Academy of Sciences.
"Caulobacter is an excellent model system to study one of the most important questions in developmental biology: What is the mechanism of an asymmetric cell division?" says Lucy Shapiro, a member of the team and Director of the Beckman Center, Stanford University School of Medicine in California.
crescentus genome and the genome of Rickettsia "may ultimately help to discriminate between the 'core' cell cycle genes required for proper progression of the cell cycle and the 'peripheral' genes that are cell-cycle regulated but not needed for full viability in C.
www.genomenewsnetwork.org /articles/03_01/Caulobacter_crescentus.shtml   (507 words)

  
 Reference.com/Encyclopedia/Caulobacter crescentus
Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams.
Caulobacter is an important model for study of the regulation of the cell cycle and cellular differentiation.
Often surviving in nutrient-poor environs, Caulobacter crescentus is a Gram-negative bacterium ubiquitous in fresh water, soil, and sea water.
www.reference.com /browse/wiki/Caulobacter   (293 words)

  
 caulobacter   (Site not responding. Last check: 2007-10-20)
Caulobacter crescentus dividing into a stalk daughter cell (top) and a motile daughter cell with a flagellum (bottom).
Caulobacter crescentus is a single-celled, small bacterium that divides asymmetrically producing cells that differ in structure and function - it is an ideal model system for the mechanisms of asymmetric cell division and has been studied thoroughly.
The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,016,942 bp encoding for 3,767 genes.
biology.kenyon.edu /Microbial_Biorealm/bacteria/proteobacteria/caulobacter/caulobacter.htm   (1028 words)

  
 James W. Gober Summary
Muir, R.E. and Gober, J. "Mutations in FlbD that Relieve Flagellum Assembly Alter the Temporal and Spatial Pattern of Developmental Transcription in Caulobacter crescentus" Mol.
Anderson, P.E., and Gober, J.W. "FlbT, the post-transcriptional regulator of flagellin synthesis in Caulobacter crescentus, interacts with the 5' untranslated region of flagellin mRNA." Mol.
Rizzo, M.F., Shapiro, L., and Gober, J.W. "Asymmetric expression of the gyrase B gene from the replication-competent chromosome in the Caulobacter crescentus predivisional cell." J.
www.biochemistry.ucla.edu /biochem/Faculty/Gober   (1032 words)

  
 Crescentin - Wikipedia, the free encyclopedia
Crescentin was recently discovered in the prokaryote Caulobacter crescentus, an aquatic bacterium which uses its crescent-shaped cells for enhanced motility.
The crescentin protein is located on the concave face of these cells and appears to be necessary for their shape, as mutants lacking the protein form rod-shaped cells.
To influence the shape of the Caulobacter cells, the helices of crescentin filaments associate with the cytoplasmic side of the cell membrane on one lateral side of the cell.
en.wikipedia.org /wiki/Crescentin   (482 words)

  
 Caulobacter crescentus Summary
Caulobacter crescentus is a Gram-negative rod-like bacterium that inhabits fresh water.
Caulobacter crescentus can be grown in the laboratory so that all the bacteria in the population undergoes division at the same time.
The optimal temperature to the growth of Caulobacter is around 30 °C and its generational period is 2.5 hours.
www.bookrags.com /Caulobacter_crescentus   (572 words)

  
 Barnard News Center
Caulobacter divides asymmetrically, with one half of the original cell developing a flagellum, a wavy "tail" attached to the cell, and the second half developing a straight "tail," or a stalk (caulis is Latin for "stalk").
The Caulobacter Meeting, along with others like it, was designed as a specialized breakout meeting for those whose research focuses on that particular bacterium.
crescentus and beyond," Poindexter discussed other bacteria that are probably regulated in their cell cycles in much the same way and that probably share many of the same genes.
www.barnard.edu /newnews/news073102.html   (274 words)

  
 Caulobacter crescentus - Wikipedia, the free encyclopedia
Caulobacter crescentus is a gram-negative, oligotrophic bacterium widely distributed in aquatic environments.
The bacterium produces the strongest natural glue known to man, which helps it attach reliably even on wet surfaces like river rocks.
The optimal temperature to the growth of Caulobacter is around 30°C and its generational period is 2.5 hours.
en.wikipedia.org /wiki/Caulobacter_crescentus   (165 words)

  
 The Manila Times Internet Edition | LIFE & TIMES > Bacterium makes nature’s strongest glue
So secure is the adhesive made by Caulobacter crescentus that the bacterium can cling to a surface even when subjected to a force equivalent to four cars balanced on a coin.
Caulobacter crescentus is usually the first organism to colonize any watery surface, from boat hulls, to water pipes, to medical catheters.
Eventually, the researchers worked out that the average C. crescentus requires around 70 newtons a square millimeter to rip it from a surface, a grip about seven times stronger than a gecko’s footpad.
www.manilatimes.net /national/2006/apr/18/yehey/life/20060418lif2.html   (327 words)

  
 Research Abstracts 2000 DOE Human Genome Program
Caulobacter crescentus is a member of the alpha subclass of the proteobacteria which also include Rickettsia, Rhizobium, Agrobacterium and Brucella species.
Caulobacters are being modified for use as a bioremediation agent for removing heavy metals from wastewater.
Caulobacter crescentus has been extensively studied because it exhibits a well-defined developmental pattern that is independent of environmental stress.
www.ornl.gov /sci/techresources/Human_Genome/publicat/00santa/119.html   (234 words)

  
 CiteULike: Three-dimensional reconstruction of the flagellar filament of Caulobacter crescentus. A flagellin lacking ...   (Site not responding. Last check: 2007-10-20)
CiteULike: Three-dimensional reconstruction of the flagellar filament of Caulobacter crescentus.
Three-dimensional reconstruction of the flagellar filament of Caulobacter crescentus.
Alignment of the primary amino acid sequences of Salmonella typhimurium (serotype i) and C. crescentus (29,000 Mr) flagellins shows that whereas there is homology at the amino and carboxyterminal ends of the two sequences, the central segment of the S. typhimurium sequence has no homology to that of C. crescentus.
www.citeulike.org /user/choonpeng/article/249018   (434 words)

  
 Low Flagellar Motor Torque and High Swimming Efficiency of Caulobacter crescentus Swarmer Cells Biophysical Journal - ...
We determined the torque of the flagellar motor of Caulobacter crescentus for different motor rotation rates by measuring the rotation rate and swimming speed of the cell body and found it to be remarkably different from that of other bacteria, such as Eschehchia coli and Vibrio alginolyticus.
Caulobacter is a bacterium that survives in very low nutrient environments (12,13).
Coulabacter crescentus CB15 wild-type and a mutant-lacking pilus.
www.findarticles.com /p/articles/mi_qa3938/is_200610/ai_n16756093   (884 words)

  
 The Future of Things (TFOT) - Nature's Super Glue
Caulobacter crescentus bacterium asymmetrically dividing into a mobile daughter cell with a flagellum (right) and a stalk daughter cell (left)
crescentus) is a gram-negative, rod-like bacterium that lives in all sorts of water sources, including lakes, streams, sea water, waste water, and even bottled spring water, attaching itself to solid substrates such as plants, other microorganisms, glass, stone, and metal.
crescentus mutants, which lacked these sugar molecules at the tip of the stalk, were no longer able to bind to surfaces.
www.tfot.info /content/view/86/57   (1107 words)

  
 Microbiology & Immunology - John Smit
Caulobacters have a two-dimensional crystalline array on their surface composed of a single secreted protein.
Secretion of the Caulobacter crescentus S-layer protein: further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins.
Linker mutagenesis of the Caulobacter crescentus S-layer protein: toward a definition of an N-terminal anchoring region and a C-terminal secretion signal and the potential for heterologous protein secretion.
www.microbiology.ubc.ca /jsmit.htm   (485 words)

  
 DOE Genomes to Life Contractor-Grantee Workshop 2005
Caulobacter crescentus is an oligotrophic α-proteobacterium with a complex cell cycle involving sessile stalked and piliated, flagellated swarmer cells.
crescentus is proposed: For both biofilm forms, the holdfast structure at the tip of a stalked cell is crucial for mediating the initial attachment.
crescentus population into a sessile, as ‘stem cells’ acting subpopulation (monolayer biofilm), which generates progeny cells capable of exploring the aqueous, oligotrophic environment by swimming motility, and a subpopulation accumulating in large mushroom structures.
doegenomestolife.org /pubs/2005abstracts/html/file85.shtml   (315 words)

  
 Indiana University Department of Biology
Yves Brun and colleagues' discovery of nature's strongest glue produced by the bacterium Caulobacter crescentus was selected as one of the Top 2006 Stories in Science by the online science magazine The Future of Things.
The discovery was also highlighted by the National Science Foundation in their "Discovery 2006: Year in Review".
Yves Brun and colleague Jay Tang from Brown University have received a $1.25 million grant from the National Institutes of Health to study the mechanisms of bacterial adhesion to surfaces, including the biosynthesis of the Caulobacter crescentus adhesive holdfast, the strongest known glue of biological origin.
www.bio.indiana.edu   (462 words)

  
 [No title]   (Site not responding. Last check: 2007-10-20)
CLEAVAGE OF CAULOBACTER PRODUCED RECOMBINANT FUSION PROTEINS FIELD OF INVENTION This invention relates to the expression and secretion of recombinant fusion proteins from Caulobacter wherein a heterologous polypeptide is fused with all or part of the surface layer protein (S-layer protein) of the bacterium.
In Caulobacter, the S-layer protein is synthesized by the cell in large quantities and the S-layer completely envelops the cell and thus appears to be a protective layer.
Caulobacter are natural inhabitants of most soil and freshwater environments and may persist in waste water treatment systems and effluents.
www.wipo.int /cgi-pct/guest/getbykey5?KEY=00/04170.000127&ELEMENT_SET=DECL   (6372 words)

  
 A dynamically localized histidine kinase controls the asymmetric distribution of polar pili proteins   (Site not responding. Last check: 2007-10-20)
The dimorphic bacterium Caulobacter crescentus undergoes an asymmetric cell division, yielding a motile swarmer cell and a sessile stalked cell.
Caulobacter crescentus cells bear an adhesive organelle at the tip of the stalk, the holdfast, raising the possibility that holdfast components may be secreted from the CpaC channel located at the tip of the stalk.
In Caulobacter, PleC resides at the piliated pole in swarmer cells and, later, is released from the pole at the swarmer-to-stalked cell transition.
www.nature.com /emboj/journal/v21/n17/full/7594672a.html   (7278 words)

  
 MIT Department of Biology: Michael Laub
Caulobacter crescentus is a powerful model for studying questions of regulation as cells are easily synchronized, cell cycle progression can be tracked by monitoring a series of morphological transitions, and a complete suite of genetic tools is available.
Although many of the major regulators in Caulobacter are known, it remains a major challenge to identify their connectivity and to understand the complete circuit which accounts for cell cycle oscillations.
The Caulobacter genome encodes 64 histidine kinases and 42 response regulators.
mit.edu /biology/www/facultyareas/facresearch/laub.html   (1136 words)

  
 Proteomics Database of Caulobacter crescentus, Experimental Procedures
A global approach was used to analyze protein synthesis and stability during the cell cycle of the bacterium Caulobacter crescentus.
This might be due to the overall low abundance of most flagellar components in this uniflagellated organism and to the fact that a large fraction of flagellar components and all chemoreceptors are membrane-integral proteins, which, due to their low solubility, could not be resolved in the first dimension of the 2-D gels.
By extrapolating this conclusion to the entire C. crescentus proteome, an estimated 5% of the cell's proteins have a half life of one cell cycle equivalent or shorter.
proteom.biomed.cas.cz /caulob/experimental.php   (9546 words)

  
 Caulobacter Crescentus   (Site not responding. Last check: 2007-10-20)
The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes.
Included are those involved in chemotaxis, outer membrane channel function, degradation of aromatic ring compounds, and the breakdown of plant-derived carbon sources, in addition to many extracytoplasmic function sigma factors, providing the organism with the ability to respond to a wide range of environmental fluctuations.
crescentus is, to our knowledge, the first free-living -class proteobacterium to be sequenced and will serve as a foundation for exploring the biology of this group of bacteria, which includes the obligate endosymbiont and human pathogen Rickettsia prowazekii, the plant pathogen Agrobacterium tumefaciens, and the bovine and human pathogen Brucella abortus.
www.biol.sc.edu /~elygen/abtract20.html   (253 words)

  
 Gulf Ecology Division
Expression of Degradative Genes of Pseudomonas putida in Caulobacter crescentus.
The recombinant plasmid RP4-TOL was transferred into Caulobacter crescentus at a high frequency, and the plasmid was maintained for at least 50 generations.
Reciprocal transfers from C. crescentus donor to P. putida or Escherichia coli recipients were less efficient and occurred at frequencies of approximately 10-3.
www.epa.gov /ged/publica/c1794.htm   (244 words)

  
 Nature's strongest glue could be used as a medical adhesive
crescentus is so small, the pulling force of 1 micronewton generates a huge stress of 70 newtons per square millimeter.
Caulobacter crescentus affixes itself to solid objects with its stalk and holdfast.
crescentus has evolved an ability to live in extremely nutrient-poor conditions, which explains its existence as a common fixture in tap water.
newsinfo.iu.edu /news/page/normal/3258.html   (527 words)

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