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Topic: Cell culture

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	Cell culture - Wikipedia, the free encyclopedia
		Related to cell culture are tissue culture and organ culture, which refer to methods for growing pieces of tissue or entire organs removed from an organism in an artificial environment.
		Cell lines that originate with humans are somewhat controversial in bioethics, as they may easily outlive their parent organism and lead to the discovery of lucrative medical treatments.
		Due to the H5N1 pandemic threat, research into using cell culture for flu vaccines is being funded by the United States government in areas as diverse as using the common cold as a vector of adjuvants.
	en.wikipedia.org /wiki/Cell_culture (1118 words)

	Cell (biology) - Wikipedia, the free encyclopedia
		The cell is the structural and functional unit of all living organisms, and is sometimes called the "building block of life." Some organisms, such as bacteria, are unicellular, consisting of a single cell.
		The cytoplasm of a eukaryotic cell is surrounded by a plasma membrane.
		This membrane serves to separate and protect a cell from its surrounding environment and is made mostly from a double layer of lipids (fat-like molecules) and proteins.
	en.wikipedia.org /wiki/Cell_(biology) (3516 words)

	SIVB Terminology Listing
		Cell strain: A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells having specific properties or markers.
		Nurse culture: In the culture of plant cells, the growth of a cell or cells on a contiguous culture of different origin which in turn is in contact with the tissue culture medium.
		Recon: The viable cell reconstructed by the fusion of a karyoplast with a cytoplast.
	www.sivb.org /edu_terminology.asp (4179 words)

	Bioengineering - Cell Culture (Site not responding. Last check: 2007-09-07)
		Cultures normally contain cells of one type although mixed cultures, especially of bacteria, are common in food sciences and wastewater treatment related studies.
		The cells in culture may be genetically identical (homogenous population) or may show some genetic variation (heterogeneous population).
		The original impetus for the development of cell culture was to study, under the microscope, normal physiological events such as nerve development.
	www.bioteach.ubc.ca /Bioengineering/CellCulture (1013 words)

	Beginning Molecular Biology Laboratory Manual
		The cells are grown in an atmosphere of 5-10% CO because the medium used is buffered with sodium bicarbonate/carbonic acid and the pH must be strictly maintained.
		Cultures should be examined daily, observing the morphology, the color of the medium and the density of the cells.
		Prepare a cell suspension, either directly from a cell culture or from a concentrated or diluted suspension (depending on the cell density) and combine 20 μl of cells with 20 μl of trypan blue suspension (0.4%).
	www.research.umbc.edu /~jwolf/method5.htm (2909 words)

	Cell Phone Culture
		I believe that cell phones, by allowing people to insulate their private interactions from the culture around them, will encourage a kind of “walled garden” of micro-cultures that is complex, but exclusive.
		Cell phone penetration is expected to outstrip computer penetration in Asia for several years, and most cell phones are used by people under 25.
		Cell phones seem similar, in that the object itself was not created to fill a specific need, but rather has potentialities that are realized differently by different people or are exploited differently by corporate rivals.
	web.mit.edu /comm-forum/forums/cell_phone_culture.htm (3589 words)

	Cell Culture by Ashley Mack
		Cell cultures are used as a research technique in fundamental cytology to investigate normal cell metabolism.
		Cell cultures have been used as a medium for the growth of viruses, the detection of viruses and the diagnosis of viral types (Woodliff, 1964).
		The aim of culturing cells outside the body is to isolate them from the controlling influence of the organism and to bring them into a suitable state for examination and experimentation (Woodliff, 1964).
	www.samford.edu /~gekeller/mack.html (1177 words)

	Endothelial Cell Culture (Site not responding. Last check: 2007-09-07)
		In general, cells are placed on the outsides of the fiber where they can attach and grow, while cell culture medium is continuously circulated through the interior of the fibers to provide nutrients and oxygenation.
		Endothelial cells cultured under chronic shear stress respond in a physiologic manner and form a monolayer, stop dividing, orient to the flow of medium and form tight junctions.
		Endothelial cells on the inside of the fiber co-cultured with vascular smooth muscle cells on the outside of the fiber demonstrated that as the flow rate was changed, G-protein formation and endothelin receptor expression in the smooth muscle were directly affected.
	www.fibercellsystems.com /app-endothelial.htm (1104 words)

	Cell Culture Services
		The cells we have in the core are grown in media prepared in the media lab and are routinely tested for mycoplasma and Isoenzyme analysis to ensure they are contaminant free.
		Cell culture work will be offered on an individual contractual basis.
		All cell culture is performed in a sterile environment employing aseptic techniques.
	www.lerner.ccf.org /services/cell/cellculture.php (448 words)

	Cell Biology Lab- Tissue Culture Methods
		Primary cell cultures are derived from rat, mouse and human in the Lab.
		The advantages of primary cultures are that the cells have not been "modified" in any way (other than enzymatic or physical dissociation).
		The disadvantages of primary cultures are the mixed nature of each preparation, limited lifespan of the culture and the potential contamination problems.
	anatomy.med.unsw.edu.au /cbl/research/methods/culture.htm (232 words)

	BASIC CELL CULTURE TECHNIQUES
		Cells in culture or in vitro are a useful model for studying the activity of cells in the whole organism or in vivo.
		The number of giant cells will in—crease as a culture ages or declines in "well-being." The frequency of giant cells should be relatively low and con—stant under uniform culture conditions.
		Cell populations are usually expressed as # cells/ml or # cells/culture.
	www.sci.sdsu.edu /classes/chemistry/chem467l/mardahl/basiccell.html (1605 words)

	Cell Culture Techniques Introduction
		The European Collection of Cell Cultures (ECACC) was first established within CAMR, Salisbury, UK in 1984 and has grown to be one of the largest animal cell culture collections in the world.
		Cell line DNA is typically of high quality and a viable cell offers the opportunity to examine other types of molecule, such as RNA or cellular proteins, if required at a later date.
		A further 60 cell lines from the 13th IHW collection are due to be added later this year, along with a number of recently banked lines that were originally part of the 10th IHW collection.
	www.sigmaaldrich.com /Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook.html (1040 words)

	Biodiversity - Plant Cell Culture (Site not responding. Last check: 2007-09-07)
		The main culture of interest is the cell culture, which allows for the culture of isolated cells from a very small cluster dispersed in a liquid media.
		The cell suspension is then placed on a shaker to allow the cell aggregates to disperse to form smaller clumps and single cells that are equally distributed throughout the liquid media.
		Cell cultures are also useful for the secondary metabolites they produce.
	www.bioteach.ubc.ca /Biodiversity/plantculture (1528 words)

	Official Petaka Cell Culture FAQ Huvek Hela Cell Human Monocytes - Human Epidermocytes (Site not responding. Last check: 2007-09-07)
		The Petaka™ cell culture device isolates ambient air and moisture conditions from the cells growing in the inside chamber, avoiding excess of oxygen and slowing down of pH changes.
		The experimental average yield from tests performed in laboratories is about 750,000 cells /ml of media, equal or slightly better than the average yields of the classic T-flask cell culture device which yields 600,000 cells / ml of media.
		While cells cultured in a Petaka can be incubated in a water bath, a regular dry incubator without controlled CO2, or in humid incubators with controlled CO2, optimizing cell growth may may require an incubator to maintain more consistent temperature control.
	www.celartia.com /petaka_faq.html (1231 words)

	Cell Culture Freezing Media Protocol
		Cells were stored in a liquid nitrogen refrigerator, thawed quickly in a 37°C circulating water bath, diluted into complete media, pelleted for 5 minutes at 1000 rpm at 4°C, resuspended in complete media, and plated into the appropriate size culture vessel.
		Adherent cells were allowed to plate for 16 hours at which time the media was collected and centrifuged to collect the "floating cells" that did not plate.
		The cells were counted on a hemocytometer and the % viability was determined as described for suspension cells.
	www.specialtymedia.com /05Resources/Protocols/cell_freezing.htm (467 words)

	Neuronal Cell Culture (Site not responding. Last check: 2007-09-07)
		Cultures of Purkinje cells or granule cells can be maintained for up to four days without a media change at 37°C in a 95% air, 5% CO2 (v/v) humidified atmosphere.
		Primary culture of identified neurons from the visual cortex of postnatal rats.
		Purkinje cell survival is differentially regulated by metabotropic and ionotropic excitatory amino acid receptors.
	www.ionyz.com /culture.html (2070 words)

	Cell Culture Technologies news (Site not responding. Last check: 2007-09-07)
		Cell Culture Technologies is happy to announce a contribution by Ferruccio Messi, Rene Fischer and Liz Penn on the use of protein- and peptide-free minimal culture media for the maintenance and preservation of well-established mammalian cell lines.
		Cell Culture Technologies presents its fourth Starter_Kit™ and announces the successful selection, routine maintenance and banking of the monkey kidney SV40 transformed COS-1 cell line in chemically defined protein- and peptide-free InVitrus™ medium.
		Cell Culture Technologies has extended the license agreement with Merial, the worldwide leader for the manufacturing of veterinary vaccines, continuing the companies' research relationship for a minimum of two more years.
	www.cellculture.com /news.html (5591 words)

	Forum examines 'Cell Phone Culture' - MIT News Office
		Cell phones have transformed the micro-culture of classrooms and may shape the macro-culture of global youth, according to two experts in mobile communications and cultural change who gave talks at an MIT Communications Forum, "Cell Phone Culture," held on Thursday, Nov. 17, in Bartos Theater.
		The "Cell Phone" panelists were James Katz, professor of communications at Rutgers University and director of the Center for Mobile Communications Studies, and Jing Wang, S. Fang Professor of Chinese Languages and Culture at MIT.
		Wang portrayed a different cultural landscape in China, where cell phones are widely used in place of standard telephones and where transnational businesses are working to create a profitable youth music market.
	web.mit.edu /newsoffice/2005/cellphone-1130.html (519 words)

	Cell Culture Labs
		Trypsin is a proteolytic enzyme which breaks the attachment between the cells and the growing surface of the culture flasks.
		That is, you will be detaching the cells from the bottom of the flask, suspending them in 10 ml of fresh growth medium, and transferring the cells into two new, sterile culture flasks.
		The cells should be long and thin and adhered to the bottom of the flask.
	cos.colstate.edu /stokes/cell_culture.htm (907 words)

	Cell Culture Technologies welcome
		Cell Culture Technologies is a biotech company which specializes in the development and application of mammalian cell culture systems for the production of biologicals in chemically defined protein- and peptide-free culture media.
		Cell Culture Technologies is glad to announce the successful selection, routine maintenance and banking of the Syrian hamster kidney BHK21C13-2P cell line and the African green monkey kidney COS-7 cell line in the chemically defined protein- and peptide-free InVitrus™ medium.
		Cell Culture Technologies is happy to announce the outstanding results of the study published on Nature Biotechnology by Prof.
	www.cellculture.com (1761 words)

	Cell Culture Media and Cell Research by Celartia
		A scientist that has devoted more than thirty years to cell biology and medical research conceived of a company devoted to creating progressive tools and procedures for culturing cells and their byproducts.
		Using an in-depth knowledge of research methodology and an understanding of the industrial and medical needs for cultured cells, Celartia scientists concentrate their efforts in cell biology, and more specifically, creating cutting-edge tools and procedures for culturing cells in the most scientific, consistent, and efficient manner.
		Now, in the 21st century, cells are used as laboratories for biological research, as factories of desired molecules, and as keystones of tissue repair and tissue engineering.
	www.celartia.com (281 words)

	Animal Cell Culture
		The objective of this course is to teach the student the practical aspects of animal cell culture techniques which include, media preparation, aseptic techniques, routine cell maintenance and the freezing of cells for storage.
		The emphasis of this course will be on the practical aspects of cell culture techniques.
		The term paper will be a synopsia dealing with a specific area of cell culture based upon information from at least 5 original research articles from current scientific journals.
	www.cst.cmich.edu /users/mccor1fm/animal.htm (322 words)

	Cell culture
		Secondary cells were originally explanted from a donor organism, and given the correct culture conditions, divide and grow for some time in vitro, e.g.
		MRC5 cells are secondary human lung fibroblasts which undergo between 60-70 doublings before senescence.
		Unlike primary and secondary cells, immortalized cells continue to grow and divide indefinitely in vitro for as long as the correct culture conditions are maintained.
	www-micro.msb.le.ac.uk /video/culture.html (930 words)

	ATCC: The Global Bioresource Center
		Phosphatase Detection Kit for Embryonic Stem Cells offers a reliable, sensitive, and stable assay to determine if stem cells are undifferentiated or are starting to differentiate.
		ATCC announces its award of a contract from the National Cancer Institute to design and implement a system to collect and store biological specimens and associated clinical data for use in cancer research.
		ATCC stem cell research products include fully-characterized human and mouse embryonic cells, lineage- and tissue-specific neonatally derived cells, feeder layer cells, media, sera and reagents as well as detailed protocols and scientific support.
	www.atcc.org (411 words)

	Cell Culture Solutions, LLC - Home Page
		We are a contract development company with expertise in mammalian cell culture.
		We emphasize the development and application of cell culture processes to the biopharmaceutical industry.
		Cell Culture Solutions, LLC is a company that provides contract development services in mammalian cell culture techniques, cellular process development and process optimization to the biopharmaceutical industry
	cellculturesolutions.com (106 words)

	Cell Trends, Inc. - A cell culture-based contract services laboratory.
		Cell Trends is a GLP-audited contract research laboratory with a 14-year reputation for providing quality work, prompt service, and competitive pricing.
		A broad range of contract laboratory services, utilizing tissue culture and virology-based techniques, is available to support your research objectives.
		Whether you are seeking process validation services, viral clearance services, research and development services, or a custom research project, our Laboratory team is always ready to provide exceptional personalized service with dedicated customer support.
	www.celltrends.com (151 words)

	Cell Culture LIMS (Site not responding. Last check: 2007-09-07)
		Manager Cell Culture Production, National Cell Culture Center
		The Cell Culture Laboratory Information Management System (CCLims™) is an all emcompasing solution that manages all facets of your Cell Culture Laboratory.
		No other LIMS comes close to supporting Cell Culture the way the CCLims does.
	www.cclims.com (205 words)

	Cell Culture: Your Source for Cell Culture Products and Cell Culture Solutions.
		Cell Culture: Your Source for Cell Culture Products and Cell Culture Solutions.
		These include novel animal component-free media (for CHO, hybridoma, HEK 293 cells, stem cells) for protein or antibody expression, gene therapy and tissue engineering applications.
		Both an educational and problem-solving tool, the Media Expert® is the most comprehensive source of information on cell culture media components and formulation issues.
	www.sigmaaldrich.com /Area_of_Interest/Life_Science/Cell_Culture.html (292 words)

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