| | Coupling the cell cycle to cell growth |
 | | For example, whereas the addition of penicillin to a culture of growing Escherichia coli cells inhibits cell division, the cells continue to grow and elongate into filaments, DNA replication continues, and the proper segregation of the daughter chromosomes is not affected (Fig. |
 | | The system is regulated by the conditions under which the cells are grown; that is, the growth medium determines the growth rate of a culture, which in turn dictates the frequency of all cell-cycle-related events (Jensen and Pedersen, 1990; Marr, 1991). |
 | | Not only is it counterintuitive that a cell uses a crude and chemically ill-defined parameter such as cell mass to regulate its progress through the cell cycle, but it seems likely that a central molecular parameter—or set of parameters—that reflects the growth state of the cell is used for such vital and flexible regulatory processes. |
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