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Topic: Cell incubator


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In the News (Thu 31 May 12)

  
  Cell incubator -- Facts, Info, and Encyclopedia article   (Site not responding. Last check: 2007-09-08)
A cell incubator is an apparatus used to grow and maintain (Click link for more info and facts about cell culture) cell cultures.
The incubator keeps cultures at an optimal temperature and humidity.
Cell incubators are essential for much experimental work in (Click link for more info and facts about cell biology) cell biology.
www.absoluteastronomy.com /encyclopedia/c/ce/cell_incubator.htm   (80 words)

  
 Glucose modulates cell death due to normobaric hyperoxia by maintaining cellular ATP -- Allen and White 274 (1): 159 -- ...
Cell DNA was estimated with the Hoechst dye method described by Labarca and Paigen (12).
Glucose consumption was increased greatly in cells exposed to hyperoxia (Fig.
Cell susceptibility to apoptosis by glutamine deprivation and rescue: survival and apoptotic death in cultured lymphoma-leukemia cell lines.
ajplung.physiology.org /cgi/content/full/274/1/L159   (3261 words)

  
 Learn more about Human cloning in the online encyclopedia.   (Site not responding. Last check: 2007-09-08)
Another cell with the genetic material to be cloned is fused with the original cell.
Another way of cloning is by parthenogenesis, where an unfertilized egg cell is induced to divide and grow as if it were fertilized.
After nuclear transfer, the cell would divide to form an embyro and stem cells would be removed.
www.onlineencyclopedia.org /h/hu/human_cloning.html   (2699 words)

  
 Standard Procedures for Culture of Mammalian Cells
whenever cells are moved from one vessel to another or whenever the components of culture medium are mixed together, this is done in a germ free space.
Most recently, the cells which gave rise by division to the cells you're using today, were frozen in 1995 and were kept frozen until a few days ago.
A sample of the cell suspension is applied to the slide and moves between the coverslip and the slide.
www.micro.utexas.edu /courses/bio206/mammaliancells.html   (2243 words)

  
 Growth of Hepatitis A Virus in a Mouse Liver Cell Line -- Feigelstock et al. 79 (5): 2950 -- The Journal of Virology
Cells were split 1:3 weekly; approximately one-third of the collected cells were subjected to three freeze-thaw cycles, cell debris was pelleted, and the supernatant containing HAV was stored at –70°C. HAV was titrated by an endpoint ELISA in 96-well plates containing GL37 cell monolayers.
Cells were split 1:3 weekly; one-third of the collected cells were subjected to three freeze-thaw cycles, cell debris was pelleted, and the supernatant containing HAV was stored at –70°C. HAV was titrated as described in the legend to Fig.
Cells were split 1:3 weekly; approximately one-third of the remaining cells were subjected to three freeze-thaw cycles, cellular debris was pelleted, and the HAV in the supernatant was titrated as described in the legend to Fig.
jvi.asm.org /cgi/content/full/79/5/2950   (4873 words)

  
 Millipore - Technical Library - MultiScreen Caco-2 --Optimization of Caco-2 cell growth...
Once the cells are detached and resuspended in Caco-2 cell culture medium, count the cells to determine the cell number/mL.
Frequent mixing is recommended to minimize the risk of large clumps of cells settling to the bottom of the tube, which could result in an inaccurate distribution of cells across the wells or plates.
To visualize cell growth on the filter at the completion of the TEER and lucifer yellow testing, it may be useful to stain the cell monolayers to confirm the integrity and uniformity of the cells.
www.millipore.com /publications.nsf/docs/pc1060en00   (4049 words)

  
 Measles Lab Manual - Measles Laboratory Network
Optimal growth conditions for the cells are obtained by incubating the culture in a 5% CO incubator at 37 C. (See Note in section II) Cell stocks can be prepared by using standard cryoprotection medium (section III).
Place the flasks in an incubator at 37 C. A CO2 incubator is optimal for cell growth, but if one is not available, the substitution of minimum essential medium (MEM) for DMEM is recommended or DMEM with the addition of hepes buffer and sodium bicarbonate (NaCO3) may improve buffering capacity.
After the adsorption period, observe the cells under the microscope to determine whether the sample was toxic to the cells (rounding of cells, cells floating).
www.cdc.gov /NCIDOD/DVRD/revb/measles/man.htm   (3080 words)

  
 Characterization of Melatonin Antiproliferation Effects in Normal and Neoplastic Epithelial Cells - Research ...
During the assay and when possible, cells are kept on the bottom incubator shelf to insure the least EMF exposure.
EMF fields in cell incubator: All experiments are done in the lower portion of the incubator to minimize exposure to EMF.
Further, this cell incubator has low EMF, and all experiments are done in the same cell incubator to keep EMF exposure constant for all melatonin treatments.
www.niehs.nih.gov /emfrapid/intrmurabs/barrett.html   (1499 words)

  
 Cell culture incubator - Patent 4871676
An incubator for cell culture used in the field of biotechnology, in which a driving mechanism for rotating a tray stock for supporting a plurality of trays for cell culture is provided outside an incubator housing.
The incubator further includes a motor 5, an encoder 6, a coupling 7 and a timing belt 8 which constitute a driving mechanism for driving the tray stock 3 and are disposed outside the incubator housing 1.
In addition, since the motor 5 acting as a source for generating heat is disposed outside the incubator housing 1 and an internal structure of the incubator housing 1 is symmetrical with respect to the rotation axis of the motor 5, temperature distribution in the incubator housing 1 becomes uniform.
www.freepatentsonline.com /4871676.html   (1771 words)

  
 CCR: FAQ about Fibroblast Cell Culture
Coriell Cell Repositories uses Eagle's MEM with Earle's salts for growth medium for many fibroblast cultures (see details for individual cell lines in the Web Catalog at http://ccr.coriell.org/).
If a cell culture is growing slower than expected, our first approach is to switch to a different lot of pre-tested serum and/or to alter the serum concentration by 5%.
If a cell count is required, mix the contents of the flask gently with a 1 ml pipette and remove 0.2 ml for a 1:5 diluted cell count.
locus.umdnj.edu /ccr/faq/fibro.html   (1347 words)

  
 Reducing variation in animal cell-based assays and tissue culture in general
In vitro cell based assays employing animal cells are extremely sensitive to variations in extrinsic chemical and physical parameters.
In order to develop an incubator specifically designed to minimize these variations, we have investigated the effect of temperature, osmolality and pH variations, as controlled by several commercially available carbon dioxide incubators, on cell based assays.
Following a two day incubation period, cell growth variations in culture dishes confirms cells are influenced by their location within the incubator.
www.btc-bti.com /applications/cellbasedassays.htm   (457 words)

  
 FCPPII: Project Outline
The purpose of having a fuel cell to power an incubator is such that it will replace a large and heavy battery pack which has a short run time and lengthy recharge time.
The fuel cell however, could be smaller, lighter, and will produce power as long as a supply of hydrogen will last.
The fuel cell however could have sufficient hydrogen storage such that the run time could be doubled to 6 hours, and the only ‘recharge’ the fuel cell would require is simply replacing the hydrogen supply in a matter of minutes.
www.ece.uvic.ca /499/2004b/group06/outline.html   (420 words)

  
 Cell incubator - Encyclopedia, History, Geography and Biography
Cell incubator - Encyclopedia, History, Geography and Biography
A cell incubator is an apparatus used to grow and maintain cell cultures.
Many will also regulate the carbon dioxide and oxygen content of the atmosphere inside.
www.arikah.net /encyclopedia/Cell_incubator   (102 words)

  
 Water Jacket Incubator for Live Cell Imaging
Give your cells the environmental conditions they need for optimal growth and analysis of cell biological processes during microscopic observation.
Time-lapse imaging, microscopy imaging, incubator imaging, slide, evaporation, proliferation, stable control, controlled conditions, controlled environment, warm chamber, heating stage, heat, heated stage, temperature, ph, co2, growth, curve environmental chamber providing precise and accurate temperature control Cage incubator stabilises focus drift.
An integrated temperature sensor measures the actual air temperature inside the incubator and is used to control the heating device.
www.htechc.com   (817 words)

  
 Human cloning   (Site not responding. Last check: 2007-09-08)
Another cell with the geneticmaterial to be cloned is fused with the original cell.
Another way of cloning is by parthenogenesis, where anunfertilized egg cell is induced to divide and grow as if it were fertilized.
After nuclear transfer, the cell would divide toform an embryo and stem cells would be removed.
www.therfcc.org /human-cloning-7108.html   (2867 words)

  
 Environmental Magnetic Fields and Human Breast Cancer
In this assay we follow growth over a 7-8 day period in cell culture in the presence or absence of melatonin, an oncostatic hormone that is known to inhibit breast cancer cell growth.
The use of a mu metal chamber is critical since it acts as a shield against both electric and magnetic fields that are generated during the operation of the cell incubator.
This response should be within standard prescribed limits defined by our historical data and this establishes that the cells have the proper biological response to drug treatment - cells that are unresponsive to drug/hormone treatment fail this biological check and are not used in the magnetic field experiments.
infoventures.com /emf/federal/rapid/nov95res/libur-ca.html   (1701 words)

  
 Cell incubator: Definition and Links by Encyclopedian.com - All about Cell incubator
Cell incubator: Definition and Links by Encyclopedian.com - All about Cell incubator
A cell incubator is an apparatus used grow and keep cell cultures[?].
Cell incubators are essential for much experimental work in the medical field.
www.encyclopedian.com /ce/Cell-incubator.html   (80 words)

  
 BIOGENICS   (Site not responding. Last check: 2007-09-08)
The BioTherm INC-12V is a portable incubator designed for maintaining live cell cultures, gametes and tissues at appropriate metabolic temperatures, with less than 0.05°C temperature fluctuation or variation between specimens.
The incubator can maintain specimens at a constant temperature; temperature regulation is maintainable for periods exceeding 30 hours away from mains power.
The incubator is rugged and secure, and built to be transported by third parties.
www.biogenics.com /html/products/p_05_02.html   (206 words)

  
 Medical Equipment Designer - Cost-Effective Airflow Sensors Protect Cell Incubator - May 98   (Site not responding. Last check: 2007-09-08)
The system uses sterile, disposable cassettes to grow therapeutic cells under consistent conditions, yielding a full dose of cells after 12 days of culture.
The incubator chambers are warmed to 37°C ±1°C by heat circulated from a 100-watt element through a duct by a fan.
Total fan failure would create overheating problems for both the cells and equipment, and a fan beginning to fail slows down, denying cells at the far edges of the compartment the controlled environment they need to reproduce.
www.manufacturingcenter.com /med/archives/0598/598api.htm   (444 words)

  
 Human cloning
After nuclear transfer, the cell would divide to form an embryo and stem cells would be removed.
In 2004, a group of scientists led by Woo Suk Hwang of Seoul National University in Korea claimed to have grown 30 cloned human embryos to the one-week stage, and then successfully harvested stem cells from them.
Advanced Cell Technology: The company that performed the first cloning of a human embryo
www.brainyencyclopedia.com /encyclopedia/h/hu/human_cloning.html   (2960 words)

  
 The Center for Fuel Cell Systems and Powertrain Integration   (Site not responding. Last check: 2007-09-08)
Fuel Cell Business Incubator Ramping Up key component of the Fuel Cell Center is the business incubator that will enable individuals or companies with promising related technologies to grow their ideas into commercially successful products and services.
Having already studied fuel cell technology in the classroom with Kettering Professors Etim Ubong, Craig Hoff, and Brenda Lemke, this was Ryan’s chance to explore the challenges of transferring that technology to a vehicle.
A 0-4 kW multi-stack fuel cell test stand was purchased from Schatz Energy Research Laboratory of Arcata California, and a 0-5kW Solid Oxide test stand was purchased from Advanced Measurements of Calgary, Alberta, Canada.
fuelcells.kettering.edu /meetings.html   (2175 words)

  
 Environmental Magnetic Fields and Human Breast Cancer - Research Information on the EMFRAPID Program (1994 -1998)
Thus, the growth curves of non-treated cells should be nearly identical across all incubators, which can be used as a QA check.
In addition, we have taken the important precaution to grow and maintain cells for these experiments in a well-defined "low-level" magnetic field environment established by one of our exposure systems before using any cells in experiments; thus, we know the "past EMF history" of our cells prior to use in experiments.
In the human T-cell Jurkat cell line, we have data supporting the finding that receptor-ligand binding is enhanced in the present of these magnetic fields.
www.niehs.nih.gov /emfrapid/extrmurabs/liburdy2.html   (2073 words)

  
 LabAntibiotics.com - Fresh antibiotics for in vitro laboratory use   (Site not responding. Last check: 2007-09-08)
Inexpensive digital incubator that allows for the incubation of microplates,...
Shelves for the Bellco CO2 Water Jacketed Incubator.
Incubator is supplied with six but can hold up...
labantibiotics.com /CategoryItem.asp?CCode=INCU   (75 words)

  
 FCPPII: Progress Report 1
This project is to design of a power conditioning system to integrate a fuel cell to power a pre-mature infant incubator transport unit.
The fuel cell however, could be smaller, lighter, and can produce power as long as a supply of hydrogen will last.
The fuel cell however could have a run time doubled to 6 hours, and the only ‘recharge’ the fuel cell would require is simply replacing the hydrogen supply in a matter of minutes.
www.ece.uvic.ca /499/2004b/group06/pr1.html   (558 words)

  
 Cell Incubator Encyclopedia Article, Definition, History, Biography   (Site not responding. Last check: 2007-09-08)
Looking For cell incubator - Find cell incubator and more at Lycos Search.
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Search for cell incubator - Find results for cell incubator and anything else you are looking for instantly!
www.karr.net /encyclopedia/Cell_incubator   (249 words)

  
 Slice overlay assay   (Site not responding. Last check: 2007-09-08)
After enzymatic dissociation of E18 rat cortex (see dissociation protocol), cells were labeled with the carbocyanine fluorescent dye DiI (Molecular Probes; diluted 10 mg/ml in 100 percent ethanol).
The pellet is gently resuspended in 5 mls of fresh, prewarmed cell culture medium.
Shake the plate in X and Y axis gently three or four times and then put the plate in a cell culture incubator.
www.hopkinsmedicine.org /ghosh/protocols/overlayassay.html   (342 words)

  
 Stoelting Co.   (Site not responding. Last check: 2007-09-08)
The "cell fixing" mode permits the researcher to pass a high current through the culture, to "fix" the cells instantly.
Using the Delta T-4™ on the "source" side of the Quixell™ Cell Transfer System (55260) keeps cells at 37°C throughout the experiment and transfer.
When using the Delta T-4™ with the Quixell™ Cell Transfer System, order the 55265 Quixell™ Cell Incubator Package, which includes the Delta T-4™ Controller, the Quixell™ Adaptor Plate, and 20 Delta T-4™ Culture Dishes.
www.stoeltingco.com /physio/store/ViewLevel3.asp?keyword3=445   (381 words)

  
 398 Form Pages _
This includes nanoscale synthesis, analysis and characterization, single-molecule/single cell microscopy and spectroscopy, and cell culture operation.
The entire microscope system is located inside a CO cell-culture incubator that allows us to directly observe cell growth and division while imaging and characterization of nanoprobes.
water-jacketed incubator for cell culture (Farma), two microbial cell incubators (Fisher), one cell culture chamber (purifier class II safety cabinet with UV radiation), and one environ- shaker (Lab-line Orbit) for cell culture.
www.odu.edu /sci/xu/fr.htm   (1261 words)

  
 Thermo Electron Corporation --Steri-Cult R Automated CO2 Incubator   (Site not responding. Last check: 2007-09-08)
The new Steri-Cult R Automated CO2 Incubator is a winning combination of a sophisticated, robot-compatible, automated storage and plate handling system and our well known Steri-Cult CO2 Incubator - the name that brought leading edge technology, such as infrared CO2 sensors, microprocessor control, directed airflow, and HEPA filtration, to cell culture incubators.
By uniting our cell culture incubator engineering and manufacturing expertise, highly regarded laboratory automation systems experience, and the talent of a leading automation designer, Thermo Electron Corporation was able to develop a new specialized drug discovery product that reflects years of solid research and rigorous testing.
The Steri-Cult R automated system, with its unique Class 100 HEPA chamber air, filtered external humidification system, and general ease of maintenance, is ideal for your demanding ADME/Tox assays, such as Caco2, as well as other cell-based assays and general automation compatible incubation applications.
www.orionres.com /com/cda/product/detail/1,1055,22679,00.html   (255 words)

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