
	Where results make sense

Topic: Cell line

Related Topics

cell membrane

red blood cell

cell nucleus

white blood cell

stem cell

fuel cell

cell phones

cell division

solar cell

T cells

cell wall

biological cell

In the News (Thu 31 May 12)

EXECUTIVE POWER

Iran

ANALYSIS

Pakistan

Family compact

	Altogen Biosystems - Cell Transfection Technology Company
		Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell line specific transfection reagents for intracellular delivery of biomolecules.
		Our cell culture scientists can transform your cell line to stably express vector or gene of interest.
		Unfortunately, no single delivery method or transfection reagent can be applied to all types of cells; cellular cytotoxicity and transfection efficiencies vary dramatically depending on the reagent, protocol and cell type being utilized.
	www.altogen.com (367 words)

	CCR: Cell Type Descriptions (Site not responding. Last check: )
		Cell morphology of a fibroblast cell line will vary somewhat with the culture conditions and with the age of the culture or the age of the cell line, but generally the fibroblastic morphology is spindle shaped (bipolar) or stellate (multipolar); usually arranged in parallel arrays at confluence in contact-inhibited cultures.
		B-Lymphoblast cell lines are established at CCR by transformation of B-lymphocytes isolated as peripheral blood mononuclear cells with Epstein-Barr virus (EBV) using phytohemaglutinin as a mitogen or identified by a submitter as a B-lymphoblast cell line.
		T-Lymphoblast cell lines are established at CCR by transformation of T-lymphocytes isolated as peripheral blood mononuclear cells with human T-cell leukemic virus (HTLV) using interleukin-2 as a mitogen or identified by a submitter as a T-lymphoblast cell line.
	ccr.coriell.org /ccr/help/celltypedesc.html (954 words)

	IMGT/HLA Database
		The cell query form is split into two main sections, general cell queries and HLA typing and serology queries.
		Whether the cell donor is homozygous for the HLA region.
		This field details the type of cell line that was used, and is available from the various cell banks.
	www.ebi.ac.uk /imgt/hla/help/cell_help.html (763 words)

	Nikon MicroscopyU: Digital Video Gallery - Live Cell Imaging: Cell Motility
		Bovine Pulmonary Artery Endothelial Cells (BPAE Line) - The BPAE cell line was initiated in 1979 from the main stem of a pulmonary artery belonging to a young cow (Bos taurus).
		The cells are susceptibled to bovine diarrhea virus, vesicular stomatitis (Indiana strain), infectious bovine rhinotracheitis virus, bovine parvovirus, bovine adenovirus I and III, and parainfluenza virus 3.
		Cell biologists hypothesize that the ability of fibroblasts to grow so readily outside of the body is associated with their central role in the healing of wounds, which necessitates their proliferation when confronted with injury or other less than optimal conditions.
	www.microscopyu.com /moviegallery/livecellimaging/index.html (1236 words)

	4.4 Cell culture problems: identification and elimination
		Cell degeneration may lead to rounding up of cells and their detachment from the surface of the culture vessel.
		Cell cultures are grown in very rich media that can support the growth of a number of contaminants, from microbiological to other cell lines.
		After removal of the cell cultures from the cabinet, the cabinet should be swabbed down with a suitable disinfectant and the cabinet run for five minutes before introduction of another cell line.
	www.who.int /vaccines/en/poliolab/webhelp/Chapter_04/4_4_Cell_culture_problems_identification_and_elimination.htm (900 words)

	Glossary [Stem Cell Information]
		The blastocyst is a sphere made up of an outer layer of cells (the trophoblast), a fluid-filled cavity (the blastocoel), and a cluster of cells on the interior (the inner cell mass).
		When it is used to refer to cells grown in a tissue culture dish, a clone is a line of cells that is genetically identical to the originating cell.
		By combining a patient's somatic cell nucleus and an enucleated egg, a scientist may harvest embryonic stem cells from the resulting embryo that can be used to generate tissues that match a patient's body.
	stemcells.nih.gov /info/glossary.asp (1853 words)

	Cell Culture Techniques Introduction
		A cell line manufactured from a phenotypically characterized donor, who possibly has a rare genetic disorder, can be used to provide an almost limitless supply of DNA for distribution to research groups worldwide.
		Cell line DNA is typically of high quality and a viable cell offers the opportunity to examine other types of molecule, such as RNA or cellular proteins, if required at a later date.
		A further 60 cell lines from the 13th IHW collection are due to be added later this year, along with a number of recently banked lines that were originally part of the 10th IHW collection.
	www.sigmaaldrich.com /Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook.html (1040 words)

	FAQs [Stem Cell Information]
		Cells from these embryos can be used to create pluripotent stem cell "lines" —cell cultures that can be grown indefinitely in the laboratory.
		This means that embryonic stem cells may be pluripotent—that is, able to give rise to cells found in all tissues of the embryo except for germ cells rather than being merely multipotent—restricted to specific subpopulations of cell types, as adult stem cells are thought to be.
		Many of the cell lines have been characterized as embryonic stem cells by detecting expression of surface antigen markers specific to embryonic stem cells, determining if the cells are pluripotent, and demonstrating that the cells are undifferentiated.
	stemcells.nih.gov /StemCells/Templates/StemCellContentPage.aspx?NRMODE=Published&NRNODEGUID={A604DCCE-2E5F-4395-8954-FCE1C05BECED}&NRORIGINALURL=/info/faqs.asp&NRCACHEHINT=NoModifyGuest (3680 words)

	Cell Culture Techniques 12.0
		Cell culture discard in chloros (10,000) ppm must be kept in the cabinet for a minimum of two hours (preferably overnight) prior to discarding down the sink with copious amounts of water.
		Examine the cells using an inverted microscope to ensure that all the cells are detached and floating.
		But for lines that grow in clumps it may be necessary to bring the cells into a single cell suspension by centrifugation and resuspension by pipetting in a smaller volume before counting.
	www.sigmaaldrich.com /Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_12.html (5183 words)

	Stem Cell Research at UCSF \| Glossary
		Cells found mainly in the bone marrow that give rise to a number of tissues, including bone, cartilage (the lining of joints), muscle, fat and connective tissue (tissue that is in between organs and structures in the body).
		The embryonic stem cells taken from the embryo in the culture dish will be genetically identical to the body cell from which the nucleus was derived.
		Totipotent stem cells give rise to all cell types that are found in an embryo, fetus or adult organism, as well as the embryonic components of the amniotic sac and placenta.
	stemcellfacts.ucsf.edu /glossary.php (606 words)

	Cell Culture Technologies news (Site not responding. Last check: )
		Cell Culture Technologies is glad to announce the successful selection, routine maintenance, banking, authentication and certification of the murine myeloma NS0 cell line in its TurboDoma® minimal medium.
		The Jurkat E6.1 and U937 cell lines required a slower serum-withdrawal prior to cultivation in Hektor medium, and the concentration of viable cells necessary for starting a culture in said minimal medium was higher.
		Cell viability of MOLT-4/8 and PM-1 cells in Hektor minimal medium was about 92-95% throughout the culture experiment, whereas Jurkat and Jurkat E6.1 showed an average viability of 88-90%.
	www.cellculture.com /news.html (2145 words)

	ATCC: Frequently Asked Questions
		However, some cell lines never reach 100% confluency but grow in colonies or domes and should be subcultured when the cells appear to have reached a maximum density for that particular cell line and vessel.
		Answer: Suspension cell lines can be fed by the simple addition of fresh medium to the culture (if room is available) or by separating the cells from the old medium by centrifugation (125 x g for 5 min) with subsequent resuspension of the cell pellet in fresh medium.
		To recover the cell suspension from a glass ampule, the neck is nicked with a small file, the ampule is washed with 70% ethanol, wrapped between several folds of a sterile towel or gauze and snapped open.
	www.atcc.org /TechnicalInfo/faqCellBiology.cfm (8118 words)

	ScienceDaily: Human Embryonic Stem Cell Line Produced Without Use Of Animal Products
		Human embryonic stem cell lines are derived from specialized cells from embryos donated by infertile couples that specifically consented for their excess embryos to be used in stem cell research.
		A stem cell line is composed of a population of cells that can replicate themselves for long periods of time in vitro, meaning out of the body.
		A stem cell line is able to generate all the cells in the three basic embryonic germ layers.
	www.sciencedaily.com /releases/2006/01/060123164248.htm (806 words)

	MMRRC ES Cell Clones
		This is the minimal service, and does not include verification of the identity of the ES cell line by DNA sequencing.
		As we have occasionally encountered incorrectly identified ES cell clones, this option should be considered only if you are in a hurry and you are confident in your own ability to verify the identity of the ES cell clone.
		A total of $400, plus shipping, is charged per ES cell line that has been verified by repeat DNA sequencing ($225 for distribution of an expanded ES cell line, plus $175 for sequence confirmation).
	www.mmrrc.org /distribution/cellLines.html (301 words)

	Nikon MicroscopyU Digital Video Gallery: 3T3 Cell Motility
		When the 3T3 line was established in the early 1960s by George Todaro and Howard Green, the cells were considered somewhat of an anomaly.
		The majority of cell lines at that time induced tumors when they were injected into animal models, and it was generally accepted that for cells to be immortal, they must also be oncogenic.
		The fact that 3T3 cells could apparently grow indefinitely, while being unable to instigate tumor growth, helped scientists delineate for the first time the differences between cell mortality and a cell's ability to undergo oncogenic transformation.
	www.microscopyu.com /moviegallery/livecellimaging/3t3/index.html (847 words)

	Bioethics: Stem Cell Glossary
		Blastocyst: After the nucleus of a somatic cell is inserted into the place of the evacuated egg cell nucleus, the resulting cell begins to grow and divide.
		Clone: A line of cells that is genetically identical to the originating cell -- in this case, a stem cell.
		Somatic cell nuclear transfer: Process in which the nucleus containing half a genome is removed from a human egg cell and replaced with a nucleus containing a full genome from an adult cell of any kind from a donor.
	ncronline.org /NCR_Online/archives2/2004b/061804/061804t.htm (718 words)

	Cell Online
		Cell's Leading Edge highlights not only the most exciting research advances in biology but also the social, political, economic, and ethical concerns surrounding biomedical research worldwide.
		In keeping with Cell's emphasis on the complete story, Leading Edge article formats allow leading scientists and policy makers to present in-depth, thought-provoking evaluations of important research trends and science policies.
		These are the Top 20 Papers by download from the Cell web site for the last 30 days.
	www.cell.com (626 words)

	Cell Culture Technologies welcome (Site not responding. Last check: )
		Cell Culture Technologies is a biotech company which specializes in the development and application of mammalian cell culture systems for the production of biologicals in chemically defined protein- and peptide-free culture media.
		Cell Culture Technologies extends the ChoMaster® System license agreement with Merial S.A.S., Lyon, France, one of the worldwide leaders in the field of veterinary vaccines.
		The technology allows Merial’s scientists to routinely maintain CHO cells and to express recombinant proteins in minimal culture media free of animal derived components, proteins and peptides (news 1 – February 2006).
	www.cellculture.com /index.html (567 words)

	:: BioReliance ::
		Cell Line Characterization is a series of tests intended to confirm the identity and purity of the cell substrate.
		The testing must also verify the absence of cross-contamination and determine if the cells are prone to genetic instability.
		If the product is to be marketed internationally, then initial efforts should be placed on developing cell line characterization and testing programs that satisfy regulatory requirements and recommendations of the U.S. Food and Drug Administration (FDA), the European Medicines Evaluation Agency (EMEA), and the Japanese Ministry of Health and Welfare.
	www.bioreliance.com /cell_line_characterization_intro.html (405 words)

	Brain area, age and viral vector-specific glial cell-line-derived neurotrophic factor expression and transport in rat. ...
		Glial cell-line-derived neurotrophic factor overexpression was induced by injections of recombinant vectors derived from serotype 2 adeno-associated virus or lentivirus.
		We found that recombinant adeno-associated viral vector was able to effectively transduce mitral cells of the olfactory bulb and pyramidal cells of CA1, resulting in transport of glial cell-line-derived neurotrophic factor to the piriform cortex and to the contralateral CA1 area, respectively.
		These data suggest that the recombinant adeno-associated viral vector vector system is an optimal alternative for therapeutic glial cell-line-derived neurotrophic factor gene transduction and transport of the protein to the epileptogenic brain areas.
	www.restorativeneurology.se /publications/Kanter-Schlifke2007 (254 words)

	Privateline.com: Cellular Telephone Basics
		WFI's Mark goes on to talk about cells and sectors and the kind of antennas needed: "These days most cells are divided into sectors.
		It selects the cell to serve you by measuring signal strength, matching your mobile to the cell that has picked up the strongest signal.
		Managing handoffs or handovers, that is, moving from cell to cell, is handled in a similar manner.
	www.privateline.com /Cellbasics/Cellbasics02.html (1802 words)

	The Leukemia-Lymphoma Cell Line Factsbook by Hans G. Drexler, ISBN 0122219708 - Sanyo cell phones
		Cell Towers cuts through the rhetoric to address the complex issues of low-level RF.
		Cell phones and "instant messaging" notwithstanding, our longing for meaningful connections seems to increase in direct proportion to our accessibility.
		Cell of Cells: The Global Race to Capture and Control the Stem Cell
	www.topscellphonesstore.com /The_Leukemia-Lymphoma_Cell_Line_Factsbook_by_Hans_G._Drexler,_ISBN_0122219708/articles/909098 (1433 words)

	Cancer Genome Project
		For decades, human immortal cancer cell lines have constituted an accessible, easily usable set of biological models with which to investigate cancer biology and to explore the potential efficacy of anticancer drugs.
		In order to improve their utility the Cancer Genome Project has embarked on a systematic characterisation of the genetics and genomics of large numbers of cancer cell lines.
		Prior knowledge of their genetic abnormalities may allow more informed choice of cancer cell lines in biological experiments and drug testing and more informed interpretation of results.
	www.sanger.ac.uk /genetics/CGP/CellLines (199 words)

	ISSCR :: Stem Cell Science : Human ES Cell Lines
		With exception of the lines listed in the NIH Registry and a few stated exceptions, cell lines listed here are not eligible for NIH funding.
		Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to august 9, 2001.
		Differentiation of human embryonic stem cells to dopaminergic neurons in serum-free suspension culture.
	www.isscr.org /science/sclines.htm (441 words)

	Xcellerex - cell line development
		High-throughput (HT) clone screening is a platform which allows a variety of cell types to be rapidly screened to select high producers.
		Millions of cells can be screened, reducing cell line development time and increasing the odds of isolating the highest producers.
		Using Supercell™ and a proprietary vector, the customer’s gene of interest can be integrated into a tagged “hotspot” in a CHO DG44 cell line.
	www.xcellerex.com /cellLineDevelopment.htm (88 words)

Try your search on: Qwika (all wikis)