| | AD Diagnostic Patent (Site not responding. Last check: 2007-10-12) |
 | | The protein composition of cerebral spinal fluid is largely derived from serum proteins which leak into the subarachnoid space through imperfections in the blood brain barrier, such as the area postrema, and perhaps across the richly vascular choroid plexus, through which cerebral spinal fluid is generated as an ultrafiltrate. |
 | | By the present invention, it is preferable to photolabel a small 10 to 30 microliter aliquot of cerebral spinal fluid with radioactively-labeled photoaffinity probe (i.e., [.sup.32 P]2N.sub.3 ATP or [.sup.32 P]8N.sub.3 ATP) resulting in an approximate final concentration of 10 to 30.mu.M for detection of the Alzheimer's disease-specific 42,000M.sub.r protein. |
 | | The cerebral spinal fluid from Alzheimer's disease patients was photolabeled and the protein fraction was precipitated by ammonium sulfate at 20 to 40% saturation. |
| www.altcorp.com /AffinityLaboratory/diagnosticpat.htm (10314 words) |