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	Chimera (genetics) - Wikipedia, the free encyclopedia
		Chimeras have either 4 parents (2 fertilized eggs or early embryos are fused together) or 3 parents (a fertilized egg is fused with an unfertilized egg or a fertilized egg is fused with an extra sperm).
		Chimeras should not be confused with hybrids, which are organisms formed from two gametes (each from a different species) which formed a single zygote.
		Chimeras should also not be confused with mosaics, which are organisms with genetically different cell types, but which again originate from a single zygote.
	en.wikipedia.org /wiki/Chimera_(animal) (1002 words)

	PRINCIPAL (Site not responding. Last check: 2007-10-20)
		This chimera maintains both of the hydrolase activity and the affinity for choline, respectively, of its parent proteins.
		The activity of the immobilized chimera was determined with (p-aminophenyl)-beta-D-galactopyranoside, a synthetic substrate of beta-galactosidase.
		Gold electrodes covered with chimera protein were very stable and gave fast and reproducible electrochemical response to the addition of beta-Gal substrate in a conventional flow injection analysis system.
	www.icp.csic.es /bec/fusioneg.html (236 words)

	Cytoplasmic Localization of a Functionally Active Fanconi Anemia Group A-Green Fluorescent Protein Chimera in Human ...
		Predicted protein sizes of the various GFP chimeras were confirmed by immunoblotting, as shown for several FAA mutants in Fig 4.
		Furthermore, the cytoplasmic localization of the two FA proteins adds up to the notion that FA genes may not be directly involved in the repair of cross-linked DNA and suggests the existence of a novel pathway that confers resistance to cross-linking agents.
		Kruyt FAE, Dijkmans LM, Arwert F, Joenje H: Involvement of the Fanconi anemia protein FAC in a pathway that signals to the cyclin B/cdc2 kinase.
	www.bloodjournal.org /cgi/content/full/90/9/3288 (6523 words)

	A Region of the Myosin Rod Important for Interaction With Paramyosin in Caenorhabditis elegans Striated Muscle -- Hoppe ...
		We confirmed that the epitope was absent from chimera 7 by the lack of 5-14 staining in adults and embryos of the genotype myo-3(st386); stEx68 (not shown).
		Chimera 8, with 532 MHC A residues in the
		MHC B sequences in chimera 11 are represented by white regions, and the rod is marked with the 28-residue zones.
	www.genetics.org /cgi/content/full/156/2/631 (8449 words)

	Troponin I chimera analysis of the cardiac myofilament tension response to protein kinase A -- Westfall et al. 280 (2): ...
		Troponin I chimera analysis of the cardiac myofilament tension response to protein kinase A -- Westfall et al.
		cTnI, ssTnI, or a TnI chimera with the amino terminus of ssTnI,
		Effects of phosphorylation of troponin I and C protein in isometric tension and velocity of unloaded shortening in skinned single cardiac myocytes from rats.
	ajpcell.physiology.org /cgi/content/full/280/2/C324 (5890 words)

	Structural Determinants of SHP-2 Function and Specificity in Xenopus Mesoderm Induction -- O'Reilly and Neel 18 (1): ...
		Identification of p130cas as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST.
		Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent pathways.
		Expression, purification, and characterization of SH2-containing protein tyrosine phosphatase, SH-PTP2.
	mcb.asm.org /cgi/content/full/18/1/161 (7760 words)

	Interaction with mLin-7 Alters the Targeting of Endocytosed Transmembrane Proteins in Mammalian Epithelial Cells -- ...
		Basolateral retention of chimera proteins was examined by secondary antibody capture.
		The rate of degradation of chimera protein was determined using a pulse-chase protocol.
		(A) Endocytosis of chimera proteins from the apical side as described in Figure 7, except that 25 µM chloroquine, a weak base used to counter vesicle acidification, was included throughout the course of the experiment.
	www.molbiolcell.org /cgi/content/full/12/5/1329 (7798 words)

	Chimera on Microsoft Windows Platforms
		Since the images displayed by Chimera are designed to be manipulated interactively, hardware three-dimensional graphics acceleration is extremely desirable, especially when visualizing and manipulating large protein structures.
		If there is another version of Python already installed, when you install the Chimera version, it will overwrite the existing Windows file extension associations and that might break applications that use the other version of Python.
		It is also possible to use the command shell's FTYPE command to fix the file extension associations (run it before installing Chimera to find what the current associations are, and then restore them after the Chimera installation).
	www.cgl.ucsf.edu /chimera/1.2029/win32.html (505 words)

	A trans-acting peptide activates the yeast a1 repressor by raising its DNA-binding affinity -- Stark et al. 18 (6): ... (Site not responding. Last check: 2007-10-20)
		The binding conditions are the same as those of Figure 2, except that the incubations of DNA and protein were carried out at 4°C, as was the electrophoresis.
		proteins are present in the lanes indicated at a concentration of ~2 nM.
		proteins are present in the lanes indicated at a concentration of ~80 nM.
	embojournal.npgjournals.com /cgi/content/full/18/6/1621 (6166 words)

	Cellular Characterization of Adenylate Kinase and Its Isoform: Two-Photon Excitation Fluorescence Imaging and ...
		Table 1 summarizes the averaged diffusion coefficients of EGFP and its fusion proteins in aqueous solution and in the cell.
		integral proteins or are anchored to the lipid bilayer by a covalently
		Identification and purification of an adenylate kinase-associated protein that influences the thermolability of adenylate kinase from a temperature-sensitive adk mutant of Escherichia coli.
	www.biophysj.org /cgi/content/full/83/6/3177 (5770 words)

	A basolateral sorting signal is encoded in the alpha -subunit of Na-K-ATPase -- Muth et al. 274 (3): 688 -- AJP - Cell ...
		this chimera and accompanies it to the basolateral surface.
		Proteins were separated by electrophoresis on a 10% polyacrylamide gel.
		Biotinylated proteins were recovered on streptavidin-agarose beads, separated by electrophoresis on a 10% SDS-polyacrylamide gel, and then transferred to nitrocellulose.
	ajpcell.physiology.org /cgi/content/full/274/3/C688 (4891 words)

	Characterization and Dynamics of Aggresome Formation by a Cytosolic GFP-Chimera -- García-Mata et al. 146 (6): ...
		Chimeras containing full-length p115 (GFP-FL; A and B) or lacking a small region of the COOH terminus (GFP-766; C and D) colocalized with giantin in the Golgi region.
		overexpression of the polytopic membrane protein CFTR or inhibition
		De Silva, A., Braakman, I., Helenius, A. Posttranslational folding of vesicular stomatitis virus G protein in the ER: involvement of noncovalent and covalent complexes.
	www.jcb.org /cgi/content/full/146/6/1239 (7538 words)

	The Bacteriophage T4 Transcription Activator MotA Interacts with the Far-C-Terminal Region of the {sigma}70 Subunit of ...
		The MotA protein binds as a monomer (6, 33) to a 9-bp element
		Samples were then subjected to electrophoresis in a 6% polyacrylamide native protein gel, and proteins were detected after staining with colloidal Coomassie blue (Invitrogen).
		The fraction used in lane 2 was purified in a manner similar to that of the fraction used in lane 3.
	jb.asm.org /cgi/content/full/184/14/3957 (5047 words)

	New Insights into the Pleiotropic Drug Resistance Network from Genome-Wide Characterization of the YRR1 Transcription ...
		The end of the Pdr1p chimera is shown with the beginning of the first inhibitory region for comparison.
		The upper part of the diagram presents the results of six independent experiments in which a strain constitutively producing the gain-of-function mutant Yrr1p protein was compared with the SEY strain used as a control.
		The lower part of the diagram shows the results of three independent experiments in which the strain with the Yrr1*GAD construct was subjected to galactose induction for 4 h and compared with the wild-type FY strain, used as a control.
	mcb.asm.org /cgi/content/full/22/8/2642 (4935 words)

	Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated ...
		Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, and a dual-flavi immune response in mice -- Mathenge et al.
		Kofler, R. M., Heinz, F. and Mandl, C. Capsid protein C of tick-borne encephalitis virus tolerates large internal deletions and is a favourable target for attenuation of virulence.
		Lad, V. and Gupta, A. Inhibition of Japanese encephalitis virus maturation and transport in PS cells to cell surface by brefeldin A. Acta Virol 46, 187–190.
	jgv.sgmjournals.org /cgi/content/full/85/9/2503 (5574 words)

	Jaideep - NanoTechnology (Site not responding. Last check: 2007-10-20)
		New Route to Nanotube Synthesis for the SPRING 2003 Poster Presentation along with Lana Agha.
		Protein Images I created for Mike Kozlov using Material Studio and Chimera.
		Protein (Green1 Green2 Green3) Protein (Blue) Protein (Light Blue).
	www.utdallas.edu /~jaideep/nanotech.htm (124 words)

	Trafficking, Assembly, and Function of a Connexin43-Green Fluorescent Protein Chimera in Live Mammalian Cells -- Jordan ...
		Beyer, E.C., and Steinberg, T.H. Evidence that the gap junction protein connexin-43 is the ATP-induced pore of mouse macrophages.
		Crow, D.S., Beyer, E.C., Paul, D.L., Kobe, S.S., and Lau, A.F. Phosphorylation of connexin43 gap junction protein in uninfected and Rous sarcoma virus-transformed mammalian fibroblasts.
		Fallon, R.F., and Goodenough, D.A. Five-hour half-life of mouse liver gap-junction protein.
	www.molbiolcell.org /cgi/content/full/10/6/2033 (7447 words)

	Machen TE, et al. Cystic Fibrosis Transmembrane Conductance Regulator and H+ Permeability in Regulation of Golgi pH. ...
		We generated a plasmid encoding a modified green fluorescence protein-sialyltransferase (GFP-ST) chimera protein that was pH-sensitive and localized to the Golgi when transfected into HeLa cells and also CF and normal or cystic fibrosis transmembrane conductance regulator- (CFTR)-corrected airway epithelial cells [1].
		The chimera was extremely sensitive to pH when excited at 495 nm, and relatively insensitive to pH at about 440 nm (emission greater than 520 nm).
		A pH-sensitive, fluorescein-coupled biotin derivative (Flubi-2) was targeted to the Golgi lumen of HeLa cells by expressing an avidin-sialyltransferase chimera protein [2].
	www.joplink.net /prev/200107/15.html (3051 words)

	Entry of Newly Synthesized GLUT4 into the Insulin-responsive Storage Compartment Is Dependent upon Both the Amino ...
		trafficking proteins such as the transferrin receptor (16, 17).
		The nomenclature of the chimeras is described under "Experimental Procedures." A, GLUT4 and GLUT1 wild type constructs; B, GLUT4/GLUT1 chimeras with GLUT4 backbone (loss-of-function chimeras); C, GLUT4/GLUT1 chimeras with GLUT1 backbone (gain-of-function chimeras).
		Differentiated 3T3L1 adipocytes were electroporated with 50 µg of EGFP fusion chimeras 4–144 (A), 4–414 (B), 4–441 (C), or 4–114 (D).
	www.jbc.org /cgi/content/full/279/36/37505 (4587 words)

	LiveWare G-Protein Cells & Plasmids
		Most are based on a series of G-protein chimeras that have been developed by Bruce Conklin and colleagues at the Gladstone Institute, UCSF.
		The chimeras consist of the alpha subunit of a G
		Host Cells: CHO cells stably transfected with one of the G-protein chimera genes or the G
	www.moleculardevices.com /pages/reagents/liveware.html (404 words)

	Dex-Mtx CID
		DHFR is a well-behaved, small, monomeric protein, and the interaction between Mtx and DHFR is extensively characterized both biochemically and structurally.
		A cell-permeable heterodimeric ligand (Dex-Mtx) bridges a DNA-binding protein-receptor protein chimera (LexA-eDHFR) and a receptor-transcription activation protein chimera (rGR2-B42), effectively reconstituting a transcriptional activator and stimulating transcription of a
		Columns marked 3H- are negative controls containing one of the following sets of protein chimeras: LexA-eDHFR and B42, LexA and B42-rGR2, or LexA and B42.
	www.columbia.edu /cu/chemistry/groups/cornish/DexMtx.html (268 words)

	Available CGL Software
		Our Chimera extensible molecular modeling system has supplanted Midasplus and we are no longer distributing this software.
		One library is written in the C programming language, and other in the C++ programming language.
		ksdssp is an open source implementation of the algorithm described in Wolfgang Kabsch and Christian Sander, "Dictionary of Protein Secondary Structure: Pattern Recognition of Hydrogen-Bonded and Geometrical Features," Biopolymers, 22, 2577-2637 (1983).
	www.cgl.ucsf.edu /Overview/software.html (462 words)

	Cytoplasmic Localization of a Functionally Active Fanconi Anemia Group A-Green Fluorescent Protein Chimera in Human ...
		Articles by Kruyt, F. Articles by Joenje, H. Cytoplasmic Localization of a Functionally Active Fanconi Anemia Group A-Green Fluorescent Protein Chimera in Human 293 Cells
		fluorescent protein (GFP) hybrid resides in the cytoplasmic compartment
		FA proteins to be active in a yet to be elucidated cytoplasmic
	www.bloodjournal.org /cgi/content/abstract/90/9/3288 (780 words)

	[No title] (Site not responding. Last check: 2007-10-20)
		Ghigo JM, Weiss DS, Chen JC, Yarrow JC, Beckwith J. Localization of FtsL to the Escherichia coli septal ring.
		proteins localize to the Z ring and are required for proper septum formation in E.
		FtsL::GFP chimera protein localized as a ring in vivo.
	www.umass.edu /microbio/chime/pipe/ftsz/present/ftszinvivo.htm (60 words)

	Human TGF-beta Receptor - Biocompare Buyer's Guide (Site not responding. Last check: 2007-10-20)
		Home » Cell Biology »...(3) » Human Receptor Proteins » Human TGF-beta Receptor
		Human TGF-beta RII Recombinant Protein (Fc Chimera) (Carrier Free)
		Human TGF-beta RIIb Recombinant Protein (Fc Chimera) (Carrier Free)
	www.biocompare.com /matrix.asp?catid=10360 (69 words)

	Mouse Leptin - Biocompare Buyer's Guide (Site not responding. Last check: 2007-10-20)
		Home » Cell Biology »...(2) » Mouse Proteins & Antigens » Mouse Leptin
		Filter by Company - see product listings for ONLY companies checkmarked below
		Mouse Leptin R Recombinant Protein (Fc Chimera) (Carrier Free)
	www.biocompare.com /matrix.asp?catid=1747 (72 words)

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