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Topic: Chlamydomonas


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In the News (Mon 6 Jul 09)

  
  Chlamydomonas mating
Chlamydomonas reinhardtii, a unicellular eukaryote, is an attractive system for probing the nature of the intercellular interactions leading to gamete fusion (fertilization).
Chlamydomonas, the mating structures also appear to be covered with a glycoprotein that is involved in the adhesion of these gametes.
Chlamydomonas seems to have retained its primitive state and allows one to begin to understand some of the events involved in fertilization in an organism amenable to genetic analysis.
home.mindspring.com /~charlenef/id1.html   (636 words)

  
  Chlamydomonas mating
Chlamydomonas reinhardtii, a unicellular eukaryote, is an attractive system for probing the nature of the intercellular interactions leading to gamete fusion (fertilization).
Chlamydomonas, the mating structures also appear to be covered with a glycoprotein that is involved in the adhesion of these gametes.
Chlamydomonas seems to have retained its primitive state and allows one to begin to understand some of the events involved in fertilization in an organism amenable to genetic analysis.
www.mindspring.com /~charlenef/id1.html   (636 words)

  
 840.119:Hydrogen Production by Chlamydomonas reinhardtii cells in Sulfur-deprived Environments - OpenWetWare
The [FeFe]-hydrogenase enzymes in Chlamydomonas reinhardtii absorb light from 400-700nm with a light conversion to hydrogen of 13% to 15%.
It has been found that Chlamydomonas reinhardtii also is a low maintenance organism so because of its high efficiency, useful production mechanism, and low maintenance requirements Chlamydomonas reinhardtii appears to be a useful organism for the industrial scale production of molecular hydrogen [4].
With mass production of Chlamydomonas reinhardtii, it would be necessary to take precautions to guard against the dumping of unused algae into local lakes or ponds so that the aquatic life would survive near a plant making hydrogen from algea.
openwetware.org /wiki/840.119:Hydrogen_Production_by_Chlamydomonas_reinhardtii_cells_in_Sulfur-deprived_Environments   (2427 words)

  
 BioMed Central | Full text | Chlamydomonas flamutants reveal a link between deflagellation and intraflagellar transport
Thus, the phenotype of Chlamydomonas long flagella mutants could be a result of either an upregulation of anterograde IFT, or due to a decrease in the rate of disassembly at the tip [8-10].
Fourth, while Chlamydomonas cells resorb their flagella prior to mitosis [24], EM studies have provided evidence that flagella are detached from basal bodies, but not lost from cells, during pre-mitotic resorption [29].
Rosenbaum JL, Moulder JE, Ringo DL: Flagellar elongation and shortening in Chlamydomonas.
www.biomedcentral.com /1471-2121/4/11   (5225 words)

  
 JYI Volume Five Features: Chlamydomonas: A Powerful Tool For Biological Research
Chlamydomonas reinhardtii are unicellular eukaryotes that use two flagella for motility.
This substantially facilitates the isolation of Chlamydomonas that have mutations on interesting genes, such as those that have human counterparts or genes encoding a protein with a critical function in the cell.
Chlamydomonas studies have not yet produced cures for any of the diseases linked to defective cilia or flagella, such as polycystic kidney disease or male infertility caused by sperm flagellar motility defects.
www.jyi.org /volumes/volume5/issue7/features/tang.html   (1057 words)

  
 Vertices Fall94: Chlamydomonas
Chlamydomonas, or Chlamy for short, is seemingly the ideal organism upon which to conduct research.
Mitochondria, responsible for generating energy in the cell, are believed to have come from the alpha-purple sulfur bacterium; chloroplasts, responsible for photosynthesis in plants, are thought to have begun as cyanobacteria.
In collaboration with a visiting Japanese scientist from Sumitomo Chemicals, Ltd., the Boynton and Gillham lab has isolated a gene encoding resistance to a new herbicide developed by Sumitomo that can be used in lower concentrations to kill weeds and is less toxic to mammals.
www.duke.edu /web/vertices/update/fall94/boynton.html   (1466 words)

  
 BioMed Central | Full text | A mutation in the centriole-associated protein centrin causes genomic instability via ...
Chlamydomonas (Chlamydomonas refers to Chlamydomonas reinhardti throughout) is a unicellular green alga with genetics similar to yeast, but which, unlike yeast, has centrioles (known as basal bodies) that are virtually identical to centrioles of vertebrate cells.
Synchronized cultures of haploid Chlamydomonas cells were embedded in agarose pads under a coverslip and imaged during G1 (as judged by time relative to the light-dark cycle and by the presence of flagella on most cells) by phase contrast microscopy as previously described [24].
Indeed, in the specific case of the Chlamydomonas vfl2 mutant, it has previously been reported that even though the vfl2 mutation causes centrioles to detach from the spindle poles, the acentriolar spindles are nonetheless still bipolar [19].
www.biomedcentral.com /1741-7007/3/15   (3829 words)

  
 Chlamydomonas
Chlamydomonas reinhardtii is a unicellular green alga that has proven of great utility as a model system for the analysis of many cellular activities and especially for
Chlamydomonas may be grown in large quantities (see below), the flagella detached, isolated and used as the starting material for the purification of individual flagellar components.
Chlamydomonas cells exhibit complex swimming behaviors in response to various light stimuli allowing for the analysis flagellar regulatory pathways.
www2.uchc.edu /~king/Chlamydomonas.html   (312 words)

  
 www.seaweed.ie
Chlamydomonas is a very large genus of unicellular, motile flagellates occurring in stagnant water and on damp soil, in freshwater, seawater, and even in snow as "snow algae".
Chlamydomonas is a large genus and there is a lot of variation in reproduciton in the different species.
Chlamydomonas speices are widespread in freshwater but marine species are seeingly much less common.
seaweed.ucg.ie /Algae/chlamydomonas.lasso   (385 words)

  
 Selenoproteins and selenocysteine insertion system in the model plant cell system, Chlamydomonas reinhardtii
Chlamydomonas EST and genomic databases were analyzed for the presence of homologs of all known selenoproteins.
However, SECISearch could not be directly applied for identifying Chlamydomonas selenoprotein genes because of the differences in the length of helix 2, in the nucleotides in the apical loop and in the nucleotide preceding the core (quartet) between Chlamydomonas and animal SECIS elements.
Interestingly, analysis of the Chlamydomonas EST database revealed that the alga had at least four additional glutathione peroxidases, in which cysteine was present in place of the catalytic Sec (data not shown).
www.nature.com /emboj/journal/v21/n14/full/7594591a.html   (7220 words)

  
 Chlamydomonas reinhardtii genome sequence
Chlamydomonas plants have been useful to science for a century in both agriculture and energy research.
One species of chlamydomonas sports a red pigment -- as protection from solar damage -- and is found in alpine and arctic regions.
Completing the chlamydomonas chloroplast genome sequence is part of a larger chlamydomonas genomic initiative, spearheaded by Arthur Grossman of Stanford University, working at the Carnegie Institution of Washington.
www.news.cornell.edu /Chronicle/02/11.7.02/chlamy-Stern.html   (735 words)

  
 Chlamydomonas reinhardtii - Wikipedia, the free encyclopedia
Chlamydomonas reinhardtii is a motile single celled green alga about 10 micrometres in diameter that swims with two flagella.
Chlamydomonas is used as a model organism for research on fundamental questions in cell and molecular biology such as:
These mutants are useful tools for studying a variety of biological processes, including flagellar motility, photosynthesis, protein synthesis, etc. The attractiveness of the alga as a model organism has recently increased with the release of several genomic resources to the public domain.
en.wikipedia.org /wiki/Chlamydomonas_reinhardtii   (605 words)

  
 Centrin Scaffold in Chlamydomonas reinhardtii Revealed by Immunoelectron Microscopy -- Geimer and Melkonian 4 (7): 1253 ...
The ultrastructure of the Chlamydomonas reinhardtii basal apparatus
of centrin in the basal apparatus of Chlamydomonas reinhardtii
The ultrastructure of the Chlamydomonas reinhardtii basal apparatus: identification of an early marker of radial asymmetry inherent in the basal body.
ec.asm.org /cgi/content/full/4/7/1253   (7255 words)

  
 Endogenous Fluctuations of DNA Topology in the Chloroplast of Chlamydomonas reinhardtii -- Salvador et al. 18 (12): ...   (Site not responding. Last check: 2007-10-14)
Endogenous Fluctuations of DNA Topology in the Chloroplast of Chlamydomonas reinhardtii
Endogenous changes of DNA conformation in the 5' regions of the Chlamydomonas chloroplast atpB and psaB genes in cells grown in a 12-h dark-24-h light cycle, as determined by the cross-linking assay.
The sequence of the chloroplast atpB gene and its flanking regions in Chlamydomonas reinhardtii.
mcb.asm.org /cgi/content/full/18/12/7235   (5890 words)

  
 Chlamydomonas and Arabidopsis. A Dynamic Duo -- Gutman and Niyogi 135 (2): 607 -- PLANT PHYSIOLOGY
yeast, Chlamydomonas is unicellular, haploid, and amenable to
Maul JE, Lilly JW, Cui L, dePamphilis CW, Miller W, Harris EH, Stern DB (2002) The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats.
Sager R (1954) Mendelian and non-mendelian inheritance of streptomycin resistance in Chlamydomonas reinhardi.
www.plantphysiol.org /cgi/content/full/135/2/607   (2637 words)

  
 chlamydomonas - Search Results - MSN Encarta
This is well demonstrated in the life cycle of the alga Chlamydomonas.
Chlamydomonas reinhardtii is a motile single celled green alga about 10 micrometres in diameter that swims with two flagella.
Chlamydomonas FAQ Introductory Reference Article Lefebvre, P.A. and C.D. Silflow (1999) Chlamydomonas:
encarta.msn.com /chlamydomonas.html   (115 words)

  
 Chlamydomonas pulvinata ~ C. sp.   (Site not responding. Last check: 2007-10-14)
222 Chlamydomonas segnis Ettl [EandS] formerly Chlamydomonas intermedia (Starr and Zeikus 1987):
1638 Chlamydomonas segnis Ettl [EandS] formerly Chlamydomonas gymnogama (Starr and Zeikus 1987):
1905 Chlamydomonas segnis Ettl [EandS] formerly Chlamydomonas pallidostigmatica (Starr and Zeikus 1987):
www.zo.utexas.edu /research/utex/genus/c7.html   (1316 words)

  
 Chlamydomonas
Chlamydomonas is a genus of unicellular green algae (Chlorophyta).
These algae are found all over the world, in soil, fresh water, oceans, and even in snow on mountaintops.
Chlamydomonas is used as a model system for research on many fundamental questions in cell and molecular biology: how do cells move?
www.salk.edu /labs/pbio-u/chlamy.html   (217 words)

  
 Nikon MicroscopyU: Confocal Image Gallery - Chlamydomonas (Green Algae)
Chlamydomonas is a large genus of common green algae that are equipped with two relatively long whip-like appendages used for locomotion.
Chlamydomonas cells are comprised of a spherical cellulose membrane termed the theca and a superficially located stigma, which is sensitive to light.
Chlamydomonas move by propelling themselves with whip-like movements using two flagella or thread-like appendages, which are especially helpful during certain reproductive phases.
www.microscopyu.com /galleries/confocal/chlamydomonas.html   (396 words)

  
 The Chlamydomonas Fus1 Protein Is Present on the Mating Type plus Fusion Organelle and Required for a Critical Membrane ...
The Chlamydomonas Fus1 Protein Is Present on the Mating Type plus Fusion Organelle and Required for a Critical Membrane Adhesion Event during Fusion with minus Gametes -- Misamore et al.
Chlamydomonas, adhesion and fusion of the plasma membranes
Hunnicutt, G.R., Kosfiszer, M.G., and Snell, W.J. Cell body and flagellar agglutinins in Chlamydomonas reinhardtii: the cell body plasma membrane is a reservoir for agglutinins whose migration to the flagella is regulated by a functional barrier.
www.molbiolcell.org /cgi/content/full/14/6/2530   (7290 words)

  
 The Chloroplastic GrpE Homolog of Chlamydomonas: Two Isoforms Generated by Differential Splicing -- Schroda et al. 13 ...
Chlamydomonas chloroplasts were isolated and treated for 10 min with the uncoupler FCCP (20 µM) before lysis and with apyrase (2 units/mL) after lysis to eliminate endogenous ATP.
Chlamydomonas chloroplasts were isolated to high purity, and one-half was incubated with 20 µM FCCP.
A Chlamydomonas culture was split into two fractions, one of which was left at 25°C in continuous light (CL; lanes 1 and 2), the other heat-shocked at 40°C for 55 min (HS; lanes 3 to 6).
www.plantcell.org /cgi/content/full/13/12/2823   (8103 words)

  
 White Mutants of Chlamydomonas reinhardtii Are Defective in Phytoene Synthase -- McCarthy et al. 168 (3): 1249 -- ...
White Mutants of Chlamydomonas reinhardtii Are Defective in Phytoene Synthase -- McCarthy et al.
the PSY gene from the Chlamydomonas wild-type and mutant alleles,
Isolation of a Chlamydomonas pds mutant by reverse
www.genetics.org /cgi/content/full/168/3/1249   (4040 words)

  
 PID - Chlamydomonas Appearance   (Site not responding. Last check: 2007-10-14)
Chlamydomonas cells usually are encountered either as thin-walled vegetative cells or thick-walled cysts.
The cells are solitary or aggregated into randomly-organized "palmelloid" colonies; algae with any nonrandom colonial organization at any stage in the life history belong to other genera.
Chlamydomonas species, especially those adapted to soil or subaerial (e.g.
megasun.bch.umontreal.ca /protists/chlamy/appearance.html   (317 words)

  
 Analysis of microtubule sliding patterns in Chlamydomonas flagellar axonemes reveals dynein activity on specific ...
Chlamydomonas strains cited in the text are listed in Table 1.
DC3, the smallest subunit of the Chlamydomonas flagellar outer dynein arm-docking complex, is a redox-sensitive Ca -binding protein.
Hyams, J. and Borisy, G. Isolated flagellar apparatus of Chlamydomonas: characterization of forward swimming and alteration of waveform and reversal of motion by Ca ions in vitro.
jcs.biologists.org /cgi/content/full/117/12/2533   (7363 words)

  
 Research in the Niyogi Lab
The unicellular green alga Chlamydomonas reinhardtii is our model organism of choice for elucidating the detailed functions of genes involved in photosynthesis and photoprotection.
An international Chlamydomonas genome project is identifying hundreds of new genes with potential roles in photosynthesis, and a challenge for the next decade is to determine the specific functions of these genes.
Following insertional mutagenesis of the Chlamydomonas nuclear genome, we are using a battery of phenotypic screens to isolate a large collection of mutants that are impaired in photosynthesis, photoprotection, and responses to ROS.
plantbio.berkeley.edu /~niyogi/genomics.html   (313 words)

  
 Chlamydomonas Sensory Rhodopsins A and B: Cellular Content and Role in Photophobic Responses -- Govorunova et al. 86 ...   (Site not responding. Last check: 2007-10-14)
Chlamydomonas Sensory Rhodopsins A and B: Cellular Content and Role in Photophobic Responses -- Govorunova et al.
A rhodopsin is the functional photoreceptor for phototaxis in the unicelullar eukaryote Chlamydomonas.
Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: simultaneous measurements of phototactic and photophobic responses.
www.biophysj.org /cgi/content/full/86/4/2342   (3826 words)

  
 A Sperm-Associated WD Repeat Protein Orthologous to Chlamydomonas PF20 Associates with Spag6, the Mammalian Orthologue ...   (Site not responding. Last check: 2007-10-14)
The 78,000 Mr intermediate chain of Chlamydomonas outer arm dynein is a microtubule-binding protein.
Sperm antigen 6 is the murine homologue of the Chlamydomonas reinhardtii central apparatus protein encoded by the PF16 locus.
Cloning and sequence analysis of the Chlamydomonas gene coding for radial spoke protein 3: flagellar mutation pf-14 is an ochre allele.
mcb.asm.org /cgi/content/full/22/22/7993   (5262 words)

  
 Research in the Niyogi Lab
The unicellular green alga Chlamydomonas reinhardtii is our model organism of choice for elucidating the detailed functions of genes involved in photosynthesis and photoprotection.
An international Chlamydomonas genome project is identifying hundreds of new genes with potential roles in photosynthesis, and a challenge for the next decade is to determine the specific functions of these genes.
Following insertional mutagenesis of the Chlamydomonas nuclear genome, we are using a battery of phenotypic screens to isolate a large collection of mutants that are impaired in photosynthesis, photoprotection, and responses to ROS.
plantbio.berkeley.edu:16080 /~niyogi/genomics.html   (313 words)

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