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Topic: Chlamydomonas reinhardtii


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In the News (Fri 4 Dec 09)

  
  Chlamydomonas reinhardtii - Wikipedia, the free encyclopedia
Chlamydomonas reinhardtii is a motile single celled green alga about 10 micrometres in diameter that swims with two flagella.
The origin of the C. reinhardtii WT laboratory strain c137 (mt+) is uncertain, but it is thought to have been collected from a field in New England in the 1940s.
Chlamydomonas is used as a model organism for research on fundamental questions in cell and molecular biology such as:
en.wikipedia.org /wiki/Chlamydomonas_reinhardtii   (632 words)

  
 Chlamydomonas reinhardtii at the Crossroads of Genomics -- Grossman et al. 2 (6): 1137 -- Eukaryotic Cell
The chloroplast atpA gene cluster in Chlamydomonas reinhardtii.
Grossman, A. Chlamydomonas reinhardtii and photosynthesis: genetics to genomics.
Cotranscription of the wild-type chloroplast atpE gene encoding the CF1/CF0 epsilon subunit with the 3' half of the rps7 gene in Chlamydomonas reinhardtii and characterization of frameshift mutations in atpE.
ec.asm.org /cgi/content/full/2/6/1137   (7325 words)

  
 Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome -- Kathir et al. 2 (2): 362 -- Eukaryotic Cell
Mutants of Chlamydomonas reinhardtii with aberrant responses to sulfur deprivation.
Identification of the gene encoding the tryptophan synthase beta-subunit from Chlamydomonas reinhardtii.
Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase.
ec.asm.org /cgi/content/full/2/2/362   (6815 words)

  
 Chlamydomonas reinhardtii genome sequence
A group led by Anastasios Melis, a professor at the University of California-Berkeley, is exploring the use of chlamydomonas as a renewable hydrogen source.
Chlamydomonas plants have been useful to science for a century in both agriculture and energy research.
Completing the chlamydomonas chloroplast genome sequence is part of a larger chlamydomonas genomic initiative, spearheaded by Arthur Grossman of Stanford University, working at the Carnegie Institution of Washington.
www.news.cornell.edu /Chronicle/02/11.7.02/chlamy-Stern.html   (735 words)

  
 The Deletion of petG in Chlamydomonas reinhardtii Disrupts the Cytochrome bf Complex -- Berthold et al. 270 (49): 29293 ...   (Site not responding. Last check: 2007-10-08)
Chlamydomonas chloroplast DNA was isolated on a cesium chloride gradient using bisbenzimide dye(11).
Chlamydomonas DNA either was isolated using a DNA miniprep (18) or was recovered from the LiCl supernatant of the RNA preparation described above.
In Chlamydomonas photosystem II, disruption of psbI, which encodes a 4.8-kDa polypeptide, causes photosensitivity of the organism and an 80-90% loss of photosystem II complex relative to wild-type levels, although photoautotrophic growth is still possible (33).
www.jbc.org /cgi/content/full/270/49/29293   (5610 words)

  
 Sequencing Genomic clones from Chlamydomonas reinhardtii at the University of Oklahoma   (Site not responding. Last check: 2007-10-08)
Sequencing Genomic clones from Chlamydomonas reinhardtii at the University of Oklahoma
Chlamydomonas reinhardtii is a unicellular green alga which swims with two flagella and has chloroplasts for photosynthesis.
Because Chlamydomonas reinhardtii is quite GC rich (~65% G+C), we have begun a pilot project to sequence several BACs in collaboration with Dr.
www.genome.ou.edu /chlamy.html   (304 words)

  
 The Circadian Clock in Chlamydomonas reinhardtii. What Is It For? What Is It Similar To? -- Mittag et al. 137 (2): 399 ...
Chlamydomonas cultures were plated onto agar medium and treated with equal amounts of UV light at different phases of a 12-h-light/12-h-dark cycle.
Bruce VG (1972) Mutants of the biological clock in Chlamydomonas reinhardtii.
Savard F, Richard C, Guertin M (1996) The Chlamydomonas reinhardtii LI818 gene represents a distant relative of the cab I/II genes that is regulated during the cell cycle and in response to illumination.
www.plantphysiol.org /cgi/content/full/137/2/399   (5347 words)

  
 Chlamydomonas - Wikipedia, the free encyclopedia
Chlamydomonas is used as a model organism for molecular biology, especially studies of flagellar motility and chloroplast dynamics, biogenesis, and genetics.
One of the many striking features of Chlamydomonas is that it contains ion channels that are directly activated by light, such as channelrhodopsin.
This page was last modified 18:53, 20 May 2006.
en.wikipedia.org /wiki/Chlamydomonas   (80 words)

  
 Mobile Self-Splicing Group I Introns from the psbA Gene of Chlamydomonas reinhardtii: Highly Efficient Homing of an ...
The chloroplast genome of the unicellular chlorophytes Chlamydomonas spp.
Sequence of the chloroplast DNA region of Chlamydomonas reinhardtii containing the gene of the large subunit of ribulose bisphosphate carboxylase and parts of its flanking genes.
Chloroplast ribosomal intron of Chlamydomonas reinhardtii: in vitro self-splicing, DNA endonuclease activity, and in vivo mobility.
mcb.asm.org /cgi/content/full/21/10/3472   (6569 words)

  
 JYI: A Putative Pyruvate: Ferredoxin Oxidoreductase Gene in Chlamydomonas reinhardtii is Expressed under Hydrogen ...   (Site not responding. Last check: 2007-10-08)
Chlamydomonas reinhardtii strain cc849 (cell wall-less) was grown in 1-liter Roux flasks photoheterotropically in TAP liquid medium (Harris 1989) supplemented with sterile 5% CO2 in air.
The C. reinhardtii putative PFOR gene sequence and the sequence of five orthologous organisms show high levels of similarity covering ~50-68% of the total protein sequence (Figure 3).
Experimental evidence that a larger percentage of pyruvate is decarboxylated to acetyl-coA in C. reinhardtii fermentative metabolism led Healey (1970) to theorize that the oxidative decarboxylation of pyruvate to acetyl-coA produces hydrogenase reductant (Bamberger et al.
www.jyi.org /volumes/volume11/issue1/articles/meuser.html   (3177 words)

  
 Chlamydomonas Center - Educational   (Site not responding. Last check: 2007-10-08)
Chlamydomonas is a genus of unicellular green algae (Chlorophyta).
Chlamydomonas is used as a model system for research on many very fundamental questions in cell and molecular biology: how do cells move?
Mike Adams is developing a teaching center for Chlamydomonas, with tested laboratory protocols, methods, resources, and links to other educational sites.
www.chlamy.org /info.html   (410 words)

  
 Chlamydomonas FAQ
Chlamydomonas swims with two flagella, and has chloroplasts for photosynthesis.
Chlamydomonas has nothing to do with Chlamydia, by the way.
Chlamydomonas is the only organism in which the nuclear, mitochondrial, and chloroplast genomes can all be specifically transformed.
www.yale.edu /rosenbaum/green_yeast.html   (366 words)

  
 Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii -- Posewitz et ...   (Site not responding. Last check: 2007-10-08)
Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii -- Posewitz et al.
Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii
Rochaix, J.D. Chlamydomonas reinhardtii as the photosynthetic yeast.
www.plantcell.org /cgi/content/full/16/8/2151   (6937 words)

  
 Phosphoregulation of an Inner Dynein Arm Complex in Chlamydomonas reinhardtii Is Altered in Phototactic Mutant Strains ...   (Site not responding. Last check: 2007-10-08)
Chlamydomonas reinhardtii strains were examined for their ability to perform positive phototaxis, which is the orientation
Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii.
Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms.
www.jcb.org /cgi/content/full/136/1/177   (8802 words)

  
 Chlamydomonas   (Site not responding. Last check: 2007-10-08)
In a lot of labs Chlamydomonas reinhardtii is used for the experiments.
Due to the fact that starch is the only carbon storage form of importance and that sucrose metabolism does not seem to play an important role, Chlamydomonas reinhardtii can be used as a simplified model for starch metabolism in higher plants.
In addition chlamydomonas shows some very interesting features that can't be directly compared with systems in higher plants, one is the so called CCM (carbon concentrating mechanism).
www-user.rhrk.uni-kl.de /~thyssen/chlamy.htm   (229 words)

  
 The Chlamydomonas reinhardtii Plastid Chromosome: Islands of Genes in a Sea of Repeats -- Maul et al. 14 (11): 2659 -- ...
The Chlamydomonas reinhardtii Plastid Chromosome: Islands of Genes in a Sea of Repeats -- Maul et al.
Chlamydomonas reinhardtii is a unicellular eukaryotic alga possessing
Boudreau, E., and Turmel, M. Gene rearrangements in Chlamydomonas chloroplast DNAs are accounted for by inversions and by the expansion/contraction of the inverted repeat.
www.plantcell.org /cgi/content/full/14/11/2659   (8406 words)

  
 The Chlamydomonas reinhardtii ODA3 Gene Encodes a Protein of the Outer Dynein Arm Docking Complex -- Koutoulis et al. ...
The Chlamydomonas reinhardtii ODA3 Gene Encodes a Protein of the Outer Dynein Arm Docking Complex -- Koutoulis et al.
Chlamydomonas miniprep DNA was obtained by digesting ~0.3 ml of packed cells with 0.5 ml of 1 mg/ml proteinase K (Boehringer
Purification and polypeptide composition of dynein ATPases from Chlamydomonas flagella.
www.jcb.org /cgi/content/full/137/5/1069   (7716 words)

  
 Characterization of Chlamydomonas reinhardtii Zygote-Specific cDNAs That Encode Novel Proteins Containing Ankyrin ...   (Site not responding. Last check: 2007-10-08)
Chlamydomonas reinhardtii is a unicellular, isogamous green alga, the sexual life cycle of which is controlled by genetically
Armbrust EV, Ferris PJ, Goodenough UW (1993) A mating type-linked gene cluster expressed in Chlamydomonas zygotes participates in the uniparental inheritance of the chloroplast genome.
Snell WJ (1982) Study of the release of cell wall degrading enzymes during adhesion of Chlamydomonas gametes.
www.plantphysiol.org /cgi/content/full/119/3/873   (6570 words)

  
 Title page for ETD etd-04082005-141028
The regulation of the photorespiratory pathway of Chlamydomonas reinhardtii during a shift from high to low CO2 conditions was investigated.
To this end, a set of C. reinhardtii cDNA sequences for known photorespiratory enzymes was assembled using the C. reinhardtii EST data and primary sequencing data.
In addition, the expression of these photorespiratory genes was reduced in cia5, a C. reinhardtii strain that lacks a transcription factor required for the induction of genes involved in the CCM.
etd.lsu.edu /docs/available/etd-04082005-141028   (309 words)

  
 Anti-Aging Medicine & Science Blog: CHLAMYDOMONAS REINHARDTII - A FUTURE HYDROGEN SOURCE?
One of the algae that has been getting lots of attention and been the focus of many resource projects is Chlamydomonas reinhardtii, a unicellular, biflagellated green algae belong to the order Volvocales.
This essay tries to give the answer to in which respect Chlamydomonas reinhardtii can be used for this purpose and what has already been done.
It is true that in the two-stage-process of producing hydrogen there is a need to increase the yield by a factor of hundred or more.
anti-ageing.us /2004/11/chlamydomonas-reinhardtii-future.html   (912 words)

  
 Portrait of a Species: Chlamydomonas reinhardtii -- Pröschold et al. 170 (4): 1601 -- Genetics   (Site not responding. Last check: 2007-10-08)
reinhardtii subclones sequenced; as indicated by the arrows, in C smithii +, one subclone had a variant position, compensatory for pairing, in helix II, while all C.
reinhardtii at the circled position in helix I. Shown in addition to the "long" form of helix I is the alternative "short" form of helix I, found in differing proportions in the standard C.
, 2003 Chlamydomonas reinhardtii at the crossroads of genomics.
www.genetics.org /cgi/content/full/170/4/1601   (5241 words)

  
 ASU SoLS Faculty: Andrew N. Webber
The chloroplast genome of Chlamydomonas can be easily transformed using a gene gun to shoot modified genes, carried on sub-micron sized gold particles, into the chloroplast.
These site-specific mutants of Chlamydomonas are characterized using a range of molecular, biochemical and biophysical techniques.
Ramesh, V.M. and Webber, A.N. Rapid isolation and purification of photosystem I (PSI) chlorophyll - binding protein from Chlamydomonas reinhardtii.
sols.asu.edu /faculty/awebber.php   (1092 words)

  
 Eyespot-Assembly Mutants in Chlamydomonas reinhardtii -- Lamb et al. 153 (2): 721 -- Genetics
Eyespot-Assembly Mutants in Chlamydomonas reinhardtii -- Lamb et al.
Communicating editor: M. Chlamydomonas reinhardtii is a single-celled green alga that
of Chlamydomonas to be characterized was an eyeless strain.
www.genetics.org /cgi/content/full/153/2/721   (4071 words)

  
 [No title]
EMBO J. Fischer, N. and Rochaix, J.D. (2001) The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii.
Plant J. Boudreau, E., Nickelsen, J., Lemaire, S. Ossenbühl, F., and Rochaix, J.-D. (2000)The Nac2 gene of Chlamydomonas reinhardtii encodes a chloroplast TPR protein involved in psbD mRNA stability, processing and/or translation.
EMBO J. Rochaix, J.-D., Fischer, N. and Hippler, M. (2000) Chloroplast site-directed mutagenesis of photosystem I in Chlamydomonas: electron transfer reactions and light sensitivity.
www.molbio.unige.ch /rochaix/papers.php   (563 words)

  
 cis- and trans-Acting Determinants for Translation of psbD mRNA in Chlamydomonas reinhardtii -- Ossenbühl and ...   (Site not responding. Last check: 2007-10-08)
A dominant mutation in the Chlamydomonas reinhardtii nuclear gene SIM30 suppresses translational defects caused by initiation codon mutations in chloroplast genes.
Nuclear mutations specifically affect the synthesis and/or degradation of the chloroplast-encoded D2 polypeptide of photosystem II in Chlamydomonas reinhardtii.
Low density membranes are associated with RNA binding proteins and thylakoids in the chloroplast of Chlamydomonas reinhardtii.
mcb.asm.org /cgi/content/full/20/21/8134   (5481 words)

  
 Chlamydomonas reinhardtii
The Chlamydomonas reinhardtii Gene Index (ChrGI) from The Institute of Genomic Research (TIGR) integrates research data from international gene research projects.
The Chlamydomonas reinhardtii EST Index is made available on the Web by the Department of Plant Gene Research at the Kazusa DNA Research Institute, Japan.
The Chlamydomonas reinhardtii EST dataset contains almost 38,000 expressed sequence tags that can be searched by similarity or by keyword.
bioresearch.ac.uk /browse/mesh/D016825.html   (195 words)

  
 JGI Chlamy v2.0 Home
Normally deriving energy from photosynthesis, with an alternative carbon source, C. reinhardtii can also thrive in total darkness.
The relative adaptability and quick generation time has made Chlamydomonas an important model for biological research.
Over the years, studies of Chlamydomonas have provided major research contributions in the areas of photosynthesis and molecular biology.
genome.jgi-psf.org /chlre2/chlre2.home.html   (66 words)

  
 [No title]
Chlamydomonas reinhardtii P450 fragments from ESTdb Sept. 19, 2001 D. Nelson Revised Dec. 9, 2002 In this set, there are 11 unique N-terminals, 8 unique C-terminals and one mid region sequence.
My estimate is that there will be at least 11 P450s in Chlamydomonas.
AV627084.1 Chlamydomonas reinhardtii 5% to 0.04% CO2 cDNA clone LCL020g12_r 5'.
drnelson.utmem.edu /chlamy.fasta.old.html   (744 words)

  
 Title page for ETD etd-0828103-114026
Chlamydomonas reinhardtii is a photosynthetic alga that has the ability to concentrate CO2 around Rubisco to achieve enhanced rates of photosynthesis in a low CO2 environment.
This dissertation presents results obtained from the generation and analysis of four high CO2 requiring mutants of C. reinhardtii.
The use of reverse genetics is a very powerful tool to dissect out the individual components of metabolic pathways.
etd.lsu.edu /docs/available/etd-0828103-114026   (291 words)

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