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| | NEUROSPORA IN TEACHING |
 | | Such isolation will not be attempted as a part of this exercise, but a partial characterization and assay of the compound will be made on the basis of paper chromatography and biological activity. |
 | | Using a glass capillary, apply a spot of the medium from flask c to a previously marked position on a line 3/4 inch from one of the long edges of a 6 x I 1-inch sheet of Whatman No. 1 filter paper. |
 | | For paper chromatography place 5 ul of 0.1% orotic acid neutralized with NaOH on Whatman No. 1 paper, develop the descending chromatogram with a solution containing 50 ml of n-butanol, 15.8 ml of 95% ethanol, 11.4 ml of formic acid and 22.8 ml of water. |
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