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Topic: Clathrin

In the News (Tue 23 Jul 19)

  Botany online: Intracellular Movements - Cytosceletons - Clathrin - Coated Vesicles
Clathrin is a protein with an extraordinary structure.
aggregation of the clathrin molecules at the surface of a coated vesicle.
Top left corner (5): insertion of clathrin into the plasmalemma, the prerequisite for the formation of new coated vesicles (according to B. If a vesicle pinches off an endomembrane and fuses with the plasmalemma, is its content exported out of the cell, the process is called exocytosis.
www.biologie.uni-hamburg.de /b-online/e25/25d.htm   (471 words)

 Receptor Mediated Endocytosis
The coated pit is a specialized region of the membrane that is coated with clathrin (for stability, to aid the transport process).
The assembly of the clathrin molecules on the pit appears to drive the pit to invaginate.
Clathrin coated pits may move in the plane of the membrane, however recent studies show that there is an "organized movement" as if the pits are tethered to cytoskeletal elements.
cellbio.utmb.edu /cellbio/recend.htm   (1989 words)

 [No title]
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Clathrin has a triskeleton structure composed of three heavy chains and three light chains that are the basic subunits of the clathrin coat.
The N-terminal of the heavy chain is known as the globular domain, and is composed of seven repeats which form a beta propeller [1].
www.ebi.ac.uk /interpro/IEntry?ac=IPR001473   (587 words)

 Bilayered Clathrin Coats on Endosomal Vacuoles Are Involved in Protein Sorting toward Lysosomes -- Sachse et al. 13 ...
Clathrin heavy chain (10-nm gold) is found on the flat, coated region of an EE.
The clathrin coat consists of a narrow electron-dense layer opposed to the endosomal limiting membrane (arrows) and a fuzzier layer facing the cytoplasm (arrowheads).
In contrast, association of clathrin with cytoplasmic vesicles and tubules is unperturbed (arrowheads).
www.molbiolcell.org /cgi/content/full/13/4/1313   (8170 words)

 Epsin Binds to Clathrin by Associating Directly with the Clathrin-terminal Domain. EVIDENCE FOR COOPERATIVE BINDING ...
and clathrin in a phosphorylation-dependent manner (19, 20).
The µ2 signal in lane h of the blot is obscured by the co-migrating GST fusion protein.
AP180 was identified on the basis of clathrin binding and polymerization properties (30).
www.jbc.org /cgi/content/full/275/9/6479   (6971 words)

 Clathrin Structure
Clathrin is a protein that plays a major role in the creation of vesicles (membrane-bound transport packages) in cells.
At the Macromolecular Crystallography Facility at the ALS, a group of scientists from the University of California, San Francisco, and MEMOREC GmbH have solved the structure of a key part of this protein involved in the lattice assembly.
The clathrin molecule (above, left) is known to take the shape of a triskelion, a figure with three bent legs.
www-als.lbl.gov /als/science/sci_archive/clathrin.html   (301 words)

 BioMed Central | Full text | Association of the Hermansky-Pudlak syndrome type-3 protein with clathrin
This consensus was determined by amino acid sequence alignment of the clathrin-binding regions in the beta subunits of adaptor proteins 1, 2, and 3 (β1, β2, β3A, and β3B), arrestin 3, and amphiphysin I and II This 'clathrin box' is sufficient for binding to the amino terminal domain of the clathrin heavy chain [29].
Clathrin is the main component of protein coats that assist in the formation of vesicles budding from the trans-Golgi network (TGN), plasma membrane, and endosomes.
Clathrin, detected by antibodies to its heavy chain, co-immunoprecipitated with HPS3 in the normal lysates but not in the HPS3 null lysates (Figure 1a, right panel).
www.biomedcentral.com /1471-2121/6/33   (4477 words)

 CLTC (clathrin heavy polypeptide)
Clathrin is the major protein constituent of the coat that surrounds organelles (cytoplasmic vesicles) to mediate selective protein transport.
Clathrin coats are involved in receptor-mediated endocytosis and intracellular trafficking and recycling of receptors, which accounts for its characteristic punctate cytoplasmic and perinuclear cellular distribution.
Structurally, clathrin is a triskelion (three-legged) shaped protein complex that is composed of a trimer of heavy chains (CLTC) each bound to a single light chain.
atlasgeneticsoncology.org /Genes/CLTCID360.html   (741 words)

 Traub Laboratory Publications
This sequence, related to the known clathrin-binding sequences in the adaptor beta subunits, amphiphysin, and beta-arrestin, facilitates the association of epsin 1 with the terminal domain of the clathrin heavy chain.
Clathrin coat formation on lysosomes is temperature dependent, displays an absolute requirement for ATP, and occurs in both semi-intact cells and onpurified lysosomes, suggesting that clathrin-coated vesicles might regulate retrograde membrane traffic out of the lysosomal compartment.
Recruitment of cytosolic clathrin onto the Golgi membranes was strictly dependent on the presence of intact AP-1.
www.pitt.edu /~traub/publications.html   (4219 words)

 Exchange of clathrin, AP2 and epsin on clathrin-coated pits in permeabilized tissue culture cells -- Yim et al. 118 ...
Clathrin (b,f) was stained with CHC5.9 antibody and AP2 (c,g) was stained with AP.6 antibody.
are responsible for the dissociation of clathrin by cytosol.
Heuser, J. and Steer, C. Trimeric binding of the 70-kD uncoating ATPase to the vertices of clathrin triskelia: a candidate intermediate in the vesicle uncoating reaction.
jcs.biologists.org /cgi/content/full/118/11/2405   (5282 words)

 Clathrin in mitotic spindles -- Okamoto et al. 279 (2): C369 -- AJP - Cell Physiology
The localization of clathrin light chain to the mitotic spindle is not affected by BFA (arrowheads).
Identification of clathrin and clathrin adaptors on tubulovesicles of gastric acid secretory (oxyntic) cells.
Clathrin self-assembly is mediated by a tandemly repeated superhelix.
ajpcell.physiology.org /cgi/content/full/279/2/C369   (3356 words)

 Real-time analysis of clathrin-mediated endocytosis during cell migration -- Rappoport and Simon 116 (5): 847 -- ...
of clathrin and transferrin at the plasma membrane (Fig.
Because the clathrin and dynamin2 are colocalized and are roughly proportional
machinery (clathrin and dynamin2) and an endocytic cargo (transferrin)
jcs.biologists.org /cgi/content/full/116/5/847   (5237 words)

 Protein Spotlight Issue 42: The Bubble's Bend
Clathrin molecules form a spherical scaffolding and accompany the membrane as it moves inwards, thus forming a spherical shell: the clathrin-coated vesicle.
The C-terminal portion of epsin binds to Eps15, its central portion binds both to clathrin adaptor protein AP2 and clathrin itself, and the ENTH domain binds to phosphatidyl inositols, with a clear preference for phosphatidylinositol-4,5-bisposphates or PtdIns(4,5)P2.
As research progresses, this specific domain is showing signs of recruiting and bridging vesicle coat components other than clathrin, and could well have a role not only in forming the container in which cargo is transported but also in transporting the cargo itself.
www.expasy.org /spotlight/back_issues/sptlt042.shtml   (1042 words)

 A Conserved Clathrin Assembly Motif Essential for Synaptic Vesicle Endocytosis -- Morgan et al. 20 (23): 8667 -- ...
C, The clathrin assembly activity of the AP180 mutants was a linear function of the number of DLL motifs.
is attributable to the inhibition of clathrin assembly in vivo.
Ye W, Lafer EM (1995a) Clathrin binding and assembly activities of expressed domains of the synapse-specific clathrin assembly protein AP-3.
www.jneurosci.org /cgi/content/full/20/23/8667   (7311 words)

 Sorting it out: AP-2 and alternate clathrin adaptors in endocytic cargo selection -- Traub 163 (2): 203 -- The Journal ...
AP-2 and clathrin (Mishra et al., 2002a; Morris and Cooper, 2001).
and clathrin by AP180 in the nucleation of clathrin lattices on membranes.
Clathrin functions in the absence of heterotetrameric adaptors and AP180-related proteins in yeast.
www.jcb.org /cgi/content/full/163/2/203   (4488 words)

 Does clathrin pull the fission trigger? -- Di Paolo and De Camilli 100 (9): 4981 -- Proceedings of the National Academy ...
This study suggests a role for clathrin heavy chain in the regulation of dynamin expression and function.
the unit elements of the clathrin lattice (20).
the "foot" of the clathrin triskelion with the clathrin adaptors.
www.pnas.org /cgi/content/full/100/9/4981   (2367 words)

 A Dominant-negative Clathrin Mutant Differentially Affects Trafficking of Molecules with Distinct Sorting Motifs in the ...
A Dominant-negative Clathrin Mutant Differentially Affects Trafficking of Molecules with Distinct Sorting Motifs in the Class II Major Histocompatibility Complex (MHC) Pathway
The role of clathrin in intracellular sorting was investigated by expression of a dominant-negative mutant form of clathrin,
The role of clathrin and routes of transport in targeting I chain-class II MHC complexes and HLA-DM to the endocytic pathway.
www.jcb.org /cgi/content/full/140/5/1023   (7521 words)

 CLATHRIN PROTEIN MOVIE SAMPLES   (Site not responding. Last check: 2007-11-04)
Clathrin protein movie - medium window, average quality (443 Kbs)
Clathrin protein movie - large window, good quality (1.9 MB)
To view the Clathrin movie at a higher quality, you may wish to download the file to your desktop.
www.hms.harvard.edu /news/clathrin   (108 words)

 Clathrin and its interactions with AP180
AP180 is a brain enriched protein essential for the recycling of synaptic vesicles after exocytosis and CALM is a ubiquitous homologue.
The adaptor binding is discussed further in other parts of these Web pages but it is not as strong as the clathrin binding, and the dominant phenotype of overexpression is a disruption of clathrin-assembly.
in the picture above point to the fact that all the clathrin is recruited to the membrane and in experiments where we titrate in more clathrin then it continues to be all recruited until there is a 1:1 stoichiometry between AP180 and clathrin on the membrane.
www.endocytosis.org /AP180/Clathrin.html   (383 words)

 [No title]
Two different adaptor protein complexes link the clathrin lattice either to the plasma membrane or to the trans golgi network.
Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat.
In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium.
www.ebi.ac.uk /interpro/IEntry?ac=IPR000547   (478 words)

 A Role for the Clathrin Assembly Domain of AP180 in Synaptic Vesicle Endocytosis -- Morgan et al. 19 (23): 10201 -- ...
Morphology of the clathrin cages was assessed by
Norris FA, Ungewickell E, Majerus PW (1995) Inositol hexakisphosphate binds to clathrin assembly protein 3 (AP-3/AP180) and inhibits clathrin cage assembly in vitro.
Ye W, Lafer EM (1995b) Clathrin binding and assembly activities of expressed domains of the synapse-specific clathrin assembly protein AP-3.
www.jneurosci.org /cgi/content/full/19/23/10201   (7172 words)

 Sharing Responsibility for Clathrin Coat Assembly
Clathrin molecules—three-pronged pinwheel-shaped proteins—form an elaborate lattice coat around the vesicles, which ultimately bud off from the membrane and transport their cargo to their cellular destination.
In a new study, Eva Schmid, Marijn Ford, McMahon, and colleagues use an impressive array of tools—biophysical, biochemical, structural, and cell biological—to shed light on the network dynamics of this “endocytic interactome.” APs orchestrate the process of cargo recruitment and assembly of the nascent vesicle and are the first hub of the endocytic network.
The accessory proteins recruit clathrin, which interacts with β-appendages and displaces accessory proteins as it accumulates and self-assembles during coat formation.
www.rxpgnews.com /research/cytology/article_4848.shtml   (895 words)

 Role of Cyclin G-associated Kinase in Uncoating Clathrin-coated Vesicles from Non-neuronal Cells -- Greener et al. 275 ...
The clathrin dissociated by Hsc70 from the coated vesicles was determined by quantifying the clathrin in the supernatant after centrifugation.
Clathrin released in 15 min from 0.4 µ
B, clathrin heavy chain released in 15 min, under identical conditions as in A, plotted as a function of increasing GAK concentration for GAK62 (square) and GAK62
www.jbc.org /cgi/content/full/275/2/1365   (4656 words)

 Clathrin-Coated Pit-Associated Proteins Are Required for Alveolar Macrophage Phagocytosis -- Perry et al. 162 (1): 380 ...
Clathrin has been implicated as a necessary component of phagocytosis (15).
pAb and endogenous clathrin HC, the molar ratio was 1 mol clathrin
Clathrin heavy chain is required for pinocytosis, the presence of large vacuoles, and development in Dictyostelium.
www.jimmunol.org /cgi/content/full/162/1/380   (5519 words)

 Clathrin-mediated endocytosis: Coated vesicle assembly
Clathrin can be recruited to membranes by a variety of proteins including AP180, epsin, amphiphysin and adaptors.
AP180 is illustrated here because this is a very efficient recruitment protein that binds to PiP2 in the plasma membrane and also induces the polymerisation of clathrin into a lattice (see AP180 pages).
As illustrated for epsin, this protein dips into the membrane and displaces lipids in one leaflet and also binds to clathrin (see Epsin pages).
www2.mrc-lmb.cam.ac.uk /groups/hmm/Endocytosis/CCVAssembly.html   (311 words)

 Spontaneous-Curvature Theory of Clathrin-Coated Membranes -- Mashl and Bruinsma 74 (6): 2862 -- Biophysical Journal
microcages of clathrin that nucleate near or at the edges of a
FIGURE 4 A clathrin network initially at neutral pH is acidified to pH 5.5-6.0, resulting in the formation of "microcages," which are devoid of cell membrane and inhibit endocytosis.
Defect-free membranes are energetically allowed to vesiculate in regions I and II, whereas dislocation unbinding is permitted in clathrin networks in regions II and III.
www.biophysj.org /cgi/content/full/74/6/2862   (6137 words)

 UniProtKB/Swiss-Prot entry P49951 [CLH1_BOVIN] Clathrin heavy chain 1
: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat.
www.expasy.org /uniprot/P49951   (355 words)

 Chemicon - Product #CBL188 - Anti-Clathrin, clone CHC5.9
This antibody reacts with the clathrin heavy chain from a diverse range of species.
In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions.
Identification of a distinct 9S form of soluble clathrin in cultured cells and tissues.
www.chemicon.com /Product/ProductDataSheet.asp?ProductItem=CBL188   (354 words)

 AP180, CALM and clathrin-mediated endocytosis
zones in the membrane and there promotes clathrin polymerisation into 'caps' or domains that approximate to the diameter of a coated vesicle
(see background, binding to membranes and polymerisation of clathrin).
and clathrin by AP180 causes nucleation of clathrin lattices on membranes.
www.endocytosis.org /AP180/AP180.html   (335 words)

 Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities ...
in the genes encoding Picalm, clathrin, or components of the
AP180 and PICALM function in the recruitment of clathrin and
The major binding partner of human PICALM is clathrin
www.pnas.org /cgi/content/full/100/14/8360   (4849 words)

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