| |
| | Method and apparatus for identifying, classifying, or quantifying protein sequences in a sample without sequencing US ... (Site not responding. Last check: 2007-10-14) |
 | | Thereby, those colonies bearing the cDNA of interest are found and isolated, the cDNA harvested and subject to sequencing. |
 | | The DNA oligomer probes for the unknown gene used for colony selection are synthesized to hybridize, preferably, only with the cDNA for the gene of interest. |
 | | Creation of these libraries is time consuming, costly, and introduces bias into the process, as it requires the cDNA in the vector to be transformed into bacteria, the bacteria arrayed as clonal colonies, and finally the growth of the individual transformed colonies. |
| patents.nimblewisdom.com /patent/6453245-Method-and-apparatus-for-identifying-classifying-or-quantifying-protein- (9410 words) |
|