
	Where results make sense

Topic: Cointegrate

In the News (Sun 27 Dec 09)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	Markerless gene replacement in Escherichia coli stimulated by a double- strand break in the chromosome -- Posfai et ...
		This cointegrate can be resolved by spontaneous recombination of the allele pair resulting in cells with either a wt or a mutant allele in the chromosome (1-3).
		Since the resolution event is rare, effective counterselection is needed to eliminate cells which retain the cointegrate structure and carry a counterselectable gene located on the inserted plasmid (4-6).
		Construction of large, chromosomal deletions is difficult because reversal of the cointegrate to wt is heavily favored due to the physical distance differences between the repeat sequences.
	nar.oxfordjournals.org /cgi/content/full/27/22/4409 (5221 words)

	Process for the incorporation of foreign DNA into the genome of dicotyledonous plants using stable cointegrate plasmids ...
		Cointegrate plasmids are obtained by mobilisation of the octopine Ti plasmid pTiB6 with the Inc.P-1 type plasmid, R772, as described by Hooykaas et al, Plasmid 4, 64-75 (1980).
		This is in conformity with expectations, because the mutation was not localised in the Ti component of the cointegrate plasmid.
		The cointegrate plasmid pAL1831 was transferred from E. coli to A. tumefaciens, whereupon for one of the transcojugants the tumour inducing capacity with different plant species was studied.
	www.freepatentsonline.com /4693976.html (4854 words)

	[No title]
		Cointegration may also take plac between more than two I(1) variables, in the sense that some linear combination of those variables is stationary.
		Cointegrating regression ADF test (CRADF): The residuals from the cointegrating mechanism are assumed to follow a first oder autoregressive process: EMBED Equation.2 where vt is a white noise process.
		Follow the EG procedure except that the static cointegrating regression is replaced by the estimation of a general ARDL model in the levels and lags of those levels of X and Y. The estimated model can be solved for its long run solution, and the residuals tested for stationarity.
	homepages.strath.ac.uk /~hbs96127/Rennes4a.doc (1321 words)

	Construction of co-integrate plasmids from plasmids of Streptomyces and Escherichia - Patent 4332900
		Cointegrate plasmid pUC1019, characterized as shown by the restriction map in FIG.
		Cointegrate plasmid pUC1020 characterized as shown by the restriction map in FIG.
		Cointegrate plasmids pUC1019 and pUC1020, and restructured plasmid pUC1024, can be isolated from their E. coli hosts by well known procedures, e.g., using the cleared lysate-isopycnic density gradient procedures described above.
	www.freepatentsonline.com /4332900.html (4346 words)

	[No title]
		Cointegration may also take place between more than two I(1) variables, in the sense that some linear combination of those variables is stationary.
		The intuitive meaning of cointegration Consider two non-stationary I(1) variables, X and Y. These may or may not contain trends in their means.
		Cointegrating regression ADF test (CRADF): The residuals from the cointegrating mechanism are assumed to follow a first order autoregressive process: EMBED Equation.2 where vt is a white noise process.
	homepages.strath.ac.uk /~hbs96127/cointeg.doc (900 words)

	Identification and Characterization of Tn4656, a Novel Class II Transposon Carrying a Set of Toluene-Degrading Genes ...
		Previous papers have clarified (i) the interchangeability of the TnpR functions among Tn21, Tn1722, and Tn4653, (ii) the identity of the amino acid sequences of the Tn4653 and Tn1722 resolvases, (iii) the interchangeability of the TnpA functions between Tn1722 and Tn4653, and (iv) the cointegration of Tn4655 by the Tn1722 or Tn4653 transposase (29, 30).
		Note that Tn1404 and Tn4653 are defective in cointegrate resolution because of a lack of crossover points (23, 30).
		transposase catalyzed cointegration of the tnpA mutants of Tn4653,
	jb.asm.org /cgi/content/full/183/21/6215 (4963 words)

	Potential of Conjugal Transfer as a Strategy for the Introduction of Recombinant Genetic Material into Strains of ...
		Cointegrates generated between a plasmid pIP501 deletion derivative (pVA797) and nonconjugative shuttle vector pSA3 were confirmed
		cointegrates were never recovered, however, and no transfer to
		Transfer of a cointegrate plasmid to strains of L.
	aem.asm.org /cgi/content/full/65/5/1910 (2296 words)

	3 Application: Money Demand
		The explanatory variables considered in the money demand equation might cointegrate not only to a money demand function but also to a stationary spread between long and short term interest rates and a stationary real interest rate.
		That's why the reduced form regression is used to identify the long-run relationships (cointegration relationships) while in a second step the model is re-written to yield a structural form, as described above.
		When the true coefficient is zero then the we would also have to assume that there is no money demand in Europe altogether and the cointegrating relationship should better be rewritten in such a way that it is normalised on a variable in whose equation the error correction term enters significantly.
	www.stat.nus.edu.sg /mirror/xplore/tutorials/seqnode4.html (1312 words)

	Natural Genomic Design in Sinorhizobium meliloti: Novel Genomic Architectures -- Guo et al. 13 (8): 1810 -- Genome ...
		To detect cointegration by the PCR method (see text), the following primers are used: for cointegration by a and b elements, primers 1 and 4 or 2 and 3; for cointegration by a and c elements, primers 1 and 5 or 2 and 6.
		The sites of cointegration of pSymB and the chromosome in strain CFNX602 and the site of cointegration of pSymA into the cointegrate formed by pSymB and the chromosome (represented in green) in strain CFNX611 are not known.
		Oligonucleotides bordering the reiterated elements participating in cointegration and excision events are indicated by arrows and numbers.
	www.genome.org /cgi/content/full/13/8/1810 (4125 words)

	The pCoo Plasmid of Enterotoxigenic Escherichia coli Is a Mosaic Cointegrate -- Froehlich et al. 187 (18): 6509 -- The ...
		The pCoo Plasmid of Enterotoxigenic Escherichia coli Is a Mosaic Cointegrate -- Froehlich et al.
		The pCoo Plasmid of Enterotoxigenic Escherichia coli Is a Mosaic Cointegrate
		the cointegrate is expected to be frequent because of the length
	jb.asm.org /cgi/content/full/187/18/6509 (5134 words)

	Welcome
		Concentrating on Tn3, cointegrate formation requires the action of the enzyme transposase, encoded by the tnpA gene, and resolution requires resolvase, encoded by the tnpR gene, and also the presence on the transposon of a resolution site and intact terminal inverted repeats.
		The full cointegrate is also illustrated in both isometric and topological view to show the relationship of the two transposons to the overall conformation of the cointegrate.
		In the case of Tn951 it is suggested by Cornelis, 1979, that it is the action of cointegrate formation that is influenced by the temperature variation.
	www.freya.u-net.com /transposon/transpson.html (4312 words)

	The pCoo plasmid of enterotoxigenic Escherichia coli is a mosaic cointegrate.
		We find that the pCoo plasmid is a cointegrate of two functional replicons, related to R64 and R100, which are joined at a 1,953-bp direct repeat of IS100.
		Recombination between these repeats in the cointegrate generates the two smaller replicons which coexist with the cointegrate in the culture.
		Subcloning of dioxygenase genes involved in biphenyl catabolism confirmed the localization of the bph genes on the wild-type plasmid and the RP4 cointegrate plasmid.
	lib.bioinfo.pl /pmid:16159784 (2918 words)

	BioMed Central \| Full text \| Random mutagenesis in Corynebacterium glutamicumATCC 13032 using an IS6100-based ...
		The two possible orientations of the cointegrate with respect to the directions of replication or transcription are present in nearly equal proportions indicating that neither orientation is favoured.
		In all cases the presence of the cointegrate was verified (data not shown).
		Nevertheless, cointegrate resolution involving either the two identical IS6100 copies or the 8-bp direct repeats might be caused by homologous recombination or by replication slippage resulting in the loss of the vector part and one or both copies of the IS6100 element.
	www.biomedcentral.com /1471-2164/7/205 (8332 words)

	Davis Biology 220 Spring 1998
		When transposition occurs between two circular DNA molecules an intermediate known as a cointegrate is believed to be formed.
		In this process, the transposon is replicated and briefly a large cointegrate (which is composed of both circular DNAs and both transposons) is formed by the transposase enzyme.
		The cointegrate is then resolved by an enzyme known as resolvase into two circular DNAs each containing a copy of the transposon.
	bioweb.wku.edu /courses/Biol220/DavisLecture34.html (822 words)

	Amazon.com: "cointegrate formation": Key Phrase page (Site not responding. Last check: 2007-10-13)
		See all pages with references to cointegrate formation.
		Cointegrate formation between the S factor and pSUP2021 can be monitored by de- manding both Kn (Tn5) and Tc (pSUP plasmid) resistance.
		Two stages of Tn3 transposition: the formation of the donor-target cointegrate and...
	www.amazon.com /phrase/cointegrate-formation (542 words)

	Handoff from recombinase to replisome: Insights from transposition -- Nakai et al. 98 (15): 8247 -- Proceedings of the ...
		MRF was originally identified as host factors needed in addition to the eight-protein system to convert STC1 to cointegrates.
		In the reaction catalyzed with crude MRF, greater than 95% of STC1 was converted to cointegrate.
		CO, position of the cointegrate; STC, position of the strand exchange product (not radiolabeled and therefore not visible).
	www.pnas.org /cgi/content/full/98/15/8247 (5708 words)

	Linker insertion mutagenesis based on IS21 transposition: isolation of an AMP-insensitive variant of catabolic ...
		The three IS21–IS21 junction constructs formed cointegrates with R751 in the presence of the helper pME3913; cointegration frequencies are calculated as previously described (Schmid et al.
		After cointegrate formation, plasmid minipreparations are digested with KpnI and XbaI, to map the pME3659::pME6 cointegrates.
		An aliquot of the cointegrate preparation is used for sequencing the IS21–arcB borders.
	peds.oxfordjournals.org /cgi/content/full/13/5/329 (3690 words)

	Active Genetic Elements Present in the Locus of Enterocyte Effacement in Escherichia coli O26 and Their Role in ...
		PCR amplimers obtained to confirm cointegrate formation obtained either with the respective recombinant plasmid or after insertion into the chromosome.
		In the upper panel, the PCR amplimers obtained confirm the cointegrate formation with plasmid pMMG-7.
		Cointegrate formation occurs between plasmid substrates containing the DR elements.
	iai.asm.org /cgi/content/full/74/7/4190 (5096 words)

	Genetic Material
		Transposition may fuse a donor and recipient into a cointegrate which later requires resolution.
		Mu transposition generates a crossover structure, which is converted by replication into a cointegrate.
		The tnpA gene codes for the transposase which sponsors the initial crossover replication and cointegrate formation.
	bioweb.wku.edu /courses/biol495G/Transposons21/Transposition3.html (330 words)

	Naturally Occurring Lactococcal Plasmid pAH90 Links Bacteriophage Resistance and Mobility Functions to a Food-Grade ...
		Shaded region corresponds to DNA derived from pAH33, and nonshaded region corresponds to DNA derived from pAH82.
		regions encoded by cointegrate pAH90 are derived from the progenitor
		was the inability to demonstrate plasmid cointegration in the
	aem.asm.org /cgi/content/full/67/2/929 (5209 words)

	SSRN-Are Inflation Expectations Rational in New Zealand? by Andrew Yeh
		Put differently, there should be no long-run difference between these two series, and innovations in one of these series should match innovations in the other.
		The subsequent tests focus on the presence or absence of a cointegrating link between actual and expectational inflation.
		A number of cointegration tests show that most New Zealand inflation expectations cointegrate with actual inflation.
	papers.ssrn.com /sol3/papers.cfm?abstract_id=671243 (408 words)

	Expression Plasmids:
		This integration forms a cointegrate plasmid that is eventually introduced into a plant cell via transformation.
		The recombinant plasmids cointegrate are selected for by plating the A.
		Incubate the wounded plant with the genetically altered A.
	www.bio.davidson.edu /people/kabernd/seminar/2002/method/msmeth/ms.htm (728 words)

	Staphylococcal tetracycline-MLSB resistance plasmid pSTE2 is the product of an RSA-mediated in vivo recombination -- ...
		cointegrates might play a role as intermediates in the evolution
		led to the cointegrate formation displayed in Figure 1.
		Staphylococcal plasmid cointegrates are formed by host- and phage-mediated rec systems that act on short regions of homology.
	jac.oxfordjournals.org /cgi/content/full/56/2/399 (1666 words)

	Assembly of large genomic segments in artificial chromosomes by homologous recombination in Escherichia coli -- Sosio ...
		The first crossover event between pMAB2 and pPAD1 leads to formation of the cointegrate (after recombination via fragment A), while the second crossover event (via fragment B) leads to the resolved cointegrate.
		the cointegrate was resolved, all the Km Cm Tc colonies we
		of growth for the resolution of cointegrates and the segregation
	nar.oxfordjournals.org /cgi/content/full/29/7/e37 (3701 words)

	Are Our FEERs Justified?
		We focus on unit root and cointegration techniques both at the individual country level and jointly using panel based estimation.
		Specifically, we test whether real exchange rates cointegrate with time series for the FEER with a coefficient of unity, so that the difference between the FEER and the real exchange rate is stationary.
		Overall the results suggest that, with the possible exception of the US, the FEER approach represents an improvement over PPP in explaining medium to long term trends in the real exchange rates of the major industrialised countries.
	ideas.repec.org /p/fth/exetec/00-02.html (368 words)

	Agrobacterium-mediated Transformation
		tumefaciens - cointegrate transformation and binary vector transformation.
		Cointegrate transformation requires homologous recombination between your tDNA/genes of interest construct and the large Agrobacterium plasmid prior to plant infection.
		Binary vectors, in which the tDNA resides on one plasmid and the Vir genes on another, came into common use in the 1980s.
	hordeum.oscs.montana.edu /class/agro.htm (833 words)

	Amazon.com: "cointegrate resolution": Key Phrase page (Site not responding. Last check: 2007-10-13)
		See all pages with references to cointegrate resolution.
		D D Transposition cointegrate r Cointegrate resolution to release transposition product and regenerate donor molecule...
		Multimer Cointegrate Resolution Systems Cointegrates are produced by replicative transposition (see Fig.
	www.amazon.com /phrase/cointegrate-resolution (463 words)

	Behavior of Tn3 Resolvase in Solution and Its Interaction with res -- Nöllmann et al. 89 (3): 1920 -- Biophysical ...
		as a cointegrate, which contains two copies of the transposon.
		the resolution of the cointegrate is achieved by a serine recombinase,
		Grindley, N. The movement of Tn3-like elements: transposition and cointegrate resolution.
	www.biophysj.org /cgi/content/full/89/3/1920 (6015 words)

	Worldwide Disseminated armA Aminoglycoside Resistance Methylase Gene Is Borne by Composite Transposon Tn1548 -- ...
		The cointegrates generated by IS6 were stable in a recA
		which led to the formation of cointegrates and which generated
		Structure and stability of Tn9-mediated cointegrates, evidence for two pathways of transposition.
	aac.asm.org /cgi/content/full/49/7/2949 (2245 words)

	Mode of Replicon Fusion Mediated by the Duplicated Insertion Sequence IS21 in Escherichia coli -- Reimmann and Haas 115 ... (Site not responding. Last check: 2007-10-13)
		formation of IS21 tandem duplications was observed in cointegrates
		in R68.45) was 100 times more active in cointegrate formation
		transposase), whereas the outer termini were not required for cointegrate
	intl.genetics.org /cgi/content/abstract/115/4/619 (432 words)

	11
		TE replicated – cointegrate structure, mlcls a and b fused together via crossing over of target sequence and TE, 2 copies
		Cointegrate formation, 1) transpoase enzyme makes single stranded breaks at each end of the transposable element and target sequence, 2) crossing over, 3) replication occurs on xover single strands
		After cointergrate formation, tresolution occurs (xover btwn sites located w/in transposon) – resolvase enzymes
	www.msu.edu /~boehmer1/Transposableelements.html (357 words)

Try your search on: Qwika (all wikis)