| | Complementary base pairing and the origin of substitution mutations |
 | | On the basis of chemical considerations and model building, the Watson−Crick concept of complementary base pairing is extended to a wider range of DNA pairs than A−T and G−C (including A−C, G−T, A−A, G−G and G−A) by invoking imino or enol tautomers (or protonated species) and syn isomers. |
 | | The virtual absence of these additional base pairs from DNA is explained in terms of the low frequency with which these unfavoured forms occur and the two-step mechanism of DNA synthesis, whereby residues are first incorporated by the DNA polymerase and then checked. |
 | | This base-pairing hypothesis is used to explain the origin, nature and level of spontaneous substitution mutations, their enhancement by base analogues, and the unique effects of certain mutator alleles. |
| www.nature.com /nature/journal/v263/n5575/abs/263285a0.html (379 words) |