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Contamination in Sequence Databases |
 | | Screens for vector contamination may also be conducted by running a sequence similarity search, such as BLAST, against other vector sequence databases, for example NCBI's vector database, or the EMVEC vector database from the European Bioinformatics Institute (EBI). |
 | | The ability to detect vector contamination using this approach alone is limited, however, because it is hard to distinguish between a single cloning site and a naturally occurring restriction site, and because the cloning process does not always recreate the sites used for cloning. |
 | | VecScreen can be used to detect contamination with many of the adapters, linkers, and PCR primers used in the most popular cDNA cloning strategies because the UniVec database includes the sequences for such oligonucleotides. |
| www.ncbi.nlm.nih.gov /VecScreen/contam.html (1053 words) |
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