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| | Olympus Microscopy Resource Center: Specialized Microscopy Techniques - Phase Contrast Illumination |
 | | To improve visibility and contrast in such specimens, microscopists often reduce the opening size of the substage condenser iris diaphragm, but this maneuver is accompanied by a serious loss of resolution and the introduction of diffraction artifacts. |
 | | Phase contrast was introduced in the 1930's for testing of telescope mirrors, and was adapted by Zeiss laboratories into a commercial microscope several years later. |
 | | Among the specimens illustrated in the phase contrast gallery are fossilized bone thin sections, stained plant tissue sections, butterfly wing scales, cells in tissue culture, algae, protozoa, and histology specimens. |
| www.olympusmicro.com /primer/techniques/phasecontrast/phaseindex.html (488 words) |
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