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Nikon MicroscopyU: Phase Contrast Microscopy - Introduction |
 | | For arguments in phase contrast microscopy, the role of the specimen in altering the optical path length (in effect, the relative phase shift) of waves passing through is of paramount importance. |
 | | Contrast is modulated by varying the properties of the phase plate, including the absorption of the metallic film (or anti-reflective coatings), the refractive index of the phase retarding material, and the thickness of the phase plate. |
 | | In effect, a series of internal cellular organelles having increasing density, such as vacuoles, cytoplasm, the interphase nucleus, and the nucleolus (or mitotic chromosomes), are typically visualized as progressively darker objects relative to a fixed reference, such as the background. |
| www.microscopyu.com /articles/phasecontrast/phasemicroscopy.html (7881 words) |
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