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Superinduction of CYP1A1 Gene Expression. REGULATION OF 2,3,7,8-TETRACHLORODIBENZO-p-DIOXIN-INDUCED DEGRADATION OF Ah ... Superinduction of CYP1A1 and inhibition of TCDD-induced down-regulation of AhR by cycloheximide. of AhR by cycloheximide correlates with the superinduction of Inhibition of the synthesis of ADPF by cycloheximide www.jbc.org /cgi/content/full/275/17/12676   (5829 words)

Cycloheximide - Wikipedia, the free encyclopedia Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with peptidyl transferase activity of the 60S ribosome, thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i. en.wikipedia.org /wiki/Cycloheximide   (216 words)

Treatment and prevention of infection with herpes simplex and related viruses - Patent 4427684 Cycloheximide is used in the treatment as well as prophylaxis of infection caused by herpes simplex hominis and related viruses. Cycloheximide is an antibiotic substance isolated from the beers of Streptomyces griseus cultures. Cycloheximide has been used in treating fungi affecting plants such as golf greens, blue grass, as a control agent against the damping off of alfalfa, or control of covered smut of wheat. www.freepatentsonline.com /4427684.html   (2776 words)

Candace Chan   (Site not responding. Last check: 2007-10-18) In the non-deflagellated group, the cycloheximide and untreated groups did not show significant change, with p > 0.05 and the null hypothesis supported, while the colchicine-treated samples showed a decrease from 10 ± 1 µm to 8 ± 2 µm and p < 0.05 calculated using the independent, two-tailed ‘Student’s’ t-test. The graph illustrated that cells treated with cycloheximide grew shorter flagella than the untreated cells because the final lengths were longer in the untreated cells. The cells treated with cycloheximide also regenerated flagella slower than the untreated cells, suggesting that the inhibition of protein synthesis from cycloheximide had a retarding effect on flagellar growth. www.owlnet.rice.edu /~ckchan/Lab1.htm   (2049 words)

The effect of chloramphenicol, ethidium bromide and cycloheximide on mortality and mitochondrial protein synthesis of ...   (Site not responding. Last check: 2007-10-18) The effects of cycloheximide, chloramphenicol and ethidium bromide on the blowfly Calliphora erythrocephala were studied. The effect of cycloheximide however, was much the same in all age groups, with inhibition of protein synthesis being 80--90% of controls. Surprisingly, cycloheximide (1--10 micrograms/fly) had little initial effect on mortality of young flies, despite almost complete blockage in the synthesis of mitochondrial proteins at these concentrations. www.arclab.org /medlineupdates/abstract_7364884.html   (462 words)

Cycloheximide Cycloheximide is an odorless, white, crystalline powder used in hospital and research laboratories as an antibiotic, a protein synthesis inhibitor, or a plant growth regulator. Cycloheximide also has broader agricultural use as a fungicide, but this use is being discontinued due to the recent findings of birth defects at low doses in animals. The highest potential for exposure in the laboratory is during the weighing of cycloheximide and during preparation of solutions of cycloheximide. www.dhs.ca.gov /ohb/HESIS/cyclohex.htm   (923 words)

BGN 12: Effects of cycloheximide treatments on prophase and metaphase cells of a (2X) Hordeum vulgare X (2X) H. ... In some prophase cells, from roots treated with cycloheximide for 2 to 3 hours, a distinct separation of groups of chromosomes was observed. In roots treated for 1 h with cycloheximide and allowed to recover for 2 hours in water metaphase cells were observed with varying numbers of non-congressed chromosomes. These observations suggest that treatment with cycloheximide disturbs the congression of the bulbosum chromosomes onto the metaphase plate and that this may eventually result in the elimination of these chromosomes (Wheatley, Noda and Kasha, unpublished observations). wheat.pw.usda.gov /ggpages/bgn/12/12p72.html   (624 words)

The Influence of Cycloheximide on Na,K-ATPase Activity in Cultured Human Lens Epithelial Cells -- Cui et al. 43 (8): ... Immediately after the preincubation period, the culture medium containing cycloheximide was removed, and the cells were permeabilized with digitonin. The influence of cycloheximide on the abundance of Na,K-ATPase Then the cells were incubated in medium containing nonradioactive methionine, with or without cycloheximide for periods up to 240 minutes. www.iovs.org /cgi/content/full/43/8/2714   (3662 words)

Banh, Mol Vis 2003; 9:323-328. Cycloheximide was also used to verify that the induction of Hsp70 takes place in lenses during heat stress. Control with cycloheximide (cycloheximide was dissolved in 0.0001% ethanol and the final concentration in culture medium was 1 μM, n=10); 4. A control group with ethanol was used to ensure that the optical changes in the cycloheximide groups (control and heat shock) were due to the effects of cycloheximide and not due to the effects of ethanol (used to prepare the cycloheximide solution). www.molvis.org /molvis/v9/a45   (3642 words)

[No title] The results indicate a decrease in flagellar length at the end of the 90 minute period for both Colchicine and Cycloheximide, while Medium I retains much of its flagellar length (11.07) when compared with the end value of the control group after 90 minutes (11.23 micrometers; not shown). Cycloheximide also showed a decrease in the flagellar length when nearing the 90 minute mark. Colchicine might have showed the greatest inhibition on the Chlamydomonas because of its speicificity for tubulin which the cycloheximide is not specific for. www2.hawaii.edu /~talaeai/school/flagella.doc   (893 words)

Cx32 mRNA in rat liver: effects of inflammation on poly(A) tail distribution and mRNA degradation -- Theodorakis and de ... Rats were injected intravenously with 1 mg/kg LPS or intraperitoneally with 6 mg/kg cycloheximide; at the indicated times livers were removed for purification of RNA. Rats were injected with saline, cycloheximide (6 mg/kg), or LPS (1 mg/kg). Nuclei isolated from rats treated with cycloheximide or LPS were incubated in presence of radiolabeled UTP. ajpregu.physiology.org /cgi/content/full/276/5/R1249   (5229 words)

Cycloheximide increases glucocorticoid-stimulated alpha -ENaC mRNA in collecting duct cells by p38 MAPK-dependent ... S]methionine in the presence or absence of cycloheximide or serum. To examine the effect of cycloheximide on MAP kinase activation, we measured total and phosphorylated p38 and JNK in MDCK-C7 lysates after treatment with either agent. Cycloheximide is a considerably weaker activator of JNK ajprenal.physiology.org /cgi/content/full/284/4/F778   (5798 words)

Type 2 Iodothyronine Deiodinase in Rat Pituitary Tumor Cells Is Inactivated in Proteasomes -- Steinsapir et al. 102 ... in the presence or absence of cycloheximide or 3,3',5'-triiodothyronine Cycloheximide (100 µM) was added to examine the half-life of D2 activity which decreased rapidly but plateaued at ~ 25% of Effects of rT3 and cycloheximide in the presence or absence of MG132 on D2 activity in GH4C1 cells. www.jci.org /cgi/content/full/102/11/1895   (4050 words)

Nonsense-Mediated Decay of Human HEXA mRNA -- Rajavel and Neufeld 21 (16): 5512 -- Molecular and Cellular Biology Stabilization of the nonsense TSD mRNA with cycloheximide. Lymphoblasts, before or after a 4-h treatment with cycloheximide, were lysed with buffer containing 0.5% NP-40 and loaded onto a 0.4 M sucrose pad to pellet nuclei. Cells were untreated, treated with cycloheximide for 4 h, or treated and then released from cycloheximide for 5 h. mcb.asm.org /cgi/content/full/21/16/5512   (5302 words)

Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal ... Cells were incubated with VP for 1 h to induce V2R-GFP internalization in the presence or absence of cycloheximide (10 µg/ml), a protein synthesis blocker. In the presence VP and cycloheximide for 0 min (G), 3 h (H), or 5 h (I), V2R-GFP was internalized and the intracellular vesicles were more loosely scattered around the nucleus (H and I) than observed in cells not treated with VP. and this reappearance was not inhibited by cycloheximide. ajpcell.physiology.org /cgi/content/full/288/6/C1390   (7888 words)

Characterization of natriuretic peptide production by adult heart atria -- Ogawa et al. 276 (6): 1977 -- AJP - Heart ... Pretreatment with cycloheximide was started at 30 min and continued to end of experiment (360 min). Effect of cycloheximide pretreatment on monensin-inhibited ANF and BNP release from isolated atria. Cycloheximide treatment of unstimulated atria resulted in a significant decrease in ANF and BNP secretion, thus showing that ajpheart.physiology.org /cgi/content/full/276/6/H1977   (5383 words)

Cycloheximide, Streptomyces griseus by A.G. Scientific, Biochemical Manufacturer   (Site not responding. Last check: 2007-10-18) Cycloheximide is an antibiotic which acts as an inhibitor of protein synthesis in eukaryotes but not prokaryotes. Cycloheximide is used primarily in the selection wild type CAN1, CYH2 and LYS2 genes. Cycloheximide is soluble in Chloroform, Ethanol and Methanol. www.agscientific.com /Item/C1189.htm   (111 words)

Environmental Protection Agency Signs and symptoms of acute exposure to cycloheximide may include increased salivation, nausea, vomiting, diarrhea, and dark, bloodstained stools. If elapsed time since ingestion of cycloheximide is unknown or suspected to be greater than 30 minutes, do not induce vomiting and proceed to Step 4. Warning: Ingestion of cycloheximide may result in sudden onset of seizures or loss of consciousness. yosemite.epa.gov /oswer/CeppoEHS.nsf/firstaid/66-81-9?OpenDocument   (662 words)

GDNF Protection against 6-OHDA: Time Dependence and Requirement for Protein Synthesis -- Kearns et al. 17 (18): 7111 -- ... Next, the effects of cycloheximide on the neuroprotective effects of GDNF were studied. Effects of cycloheximide on GDNF administration in the substantia nigra and ventral tegmental area. Tornheim K, O'Dell RG, Prosky L (1969) Effects of cycloheximide on DNA and protein synthesis in rat liver. www.jneurosci.org /cgi/content/full/17/18/7111   (4711 words)

Neuroprotective Action of Cycloheximide Involves Induction of Bcl-2 and Antioxidant Pathways -- Furukawa et al. 136 ... Effects of cycloheximide on delayed neuronal death in rat hippocampus. Cycloheximide and actinomycin D block the toxic effect of glutamic acid on PC12 cells. Dose-related effects of cycloheximide on delayed neuronal death in the gerbil hippocampus after bilateral transitory forebrain ischemia. www.jcb.org /cgi/content/full/136/5/1137   (6702 words)

Inhibition of Intracellular Degradation Increases Secretion of a Mutant Form of alpha 1-Antitrypsin Associated with ... Effect of cycloheximide and the proteasome inhibitor lactacystin on intracellular transport of Z to the extracellular compartment in the presence of cycloheximide. In the absence of cycloheximide, ~ 25% of the synthesized Z www.jci.org /cgi/content/full/101/12/2693   (4757 words)

CYCLOHEXIMIDE (ACTIDIONE) Cycloheximide (also known as actidione) is used as an selective antibiotic. But cycloheximide is highly toxic is not suitable for human use as a therapeutic antibiotic. Agricultural application also is decreasing due to the health risks. www.chemicalland21.com /lifescience/phar/CYCLOHEXIMIDE.htm   (197 words)

[No title] The presence of a protein-synthesis inhibitor, cycloheximide, in the culture medium did not significantly influence the PDGF A-chain mRNA level in normal mesothelial and malignant mesothelioma cell lines. The second peak of MAP kinase activity is markedly attenuated by the protein synthesis inhibitor cycloheximide and, consequently, is paralleled by a marked de-novo synthesis of p42 and p44 MAP kinases, as measured by immunoprecipitation of [ 35S]methionine-labeled mesangial cells and by a 700% increase in total MAP kinase protein, as detected by Western-blot analysis. The protein synthesis inhibitors S cycloheximide and puromycin reversed the glucocorticoid-mediated inhibition and suggested that new protein synthesis was necessary. www.cs.brandeis.edu /~marc/medstract/inhibitors/inhibitor.html   (3061 words)

Uveal Melanoma Expression of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) Receptors and ... Melanoma cells were incubated in cycloheximide (10 µg/mL), recombinant human TRAIL (300 ng/mL), medium, or a combination of cycloheximide and TRAIL for 36 hours at 37°C. Apoptosis was determined by flow cytometry with annexin V staining used as an indicator of apoptosis. Effect of cycloheximide treatment on TRAIL-induced apoptosis in cell lines from metastases arising in patients with uveal melanoma. OCM1 and OCM3 cells were incubated for 36 hours at 37°C in the presence of medium alone, cycloheximide (10 µg/mL) alone, or cycloheximide (10 µg/mL) and recombinant human TRAIL (300 ng/mL). www.iovs.org /cgi/content/full/45/4/1162   (3259 words)

Quinones increase gamma -glutamyl transpeptidase expression by multiple mechanisms in rat lung epithelial cells -- Liu ... Cycloheximide was purchased from Sigma (St. Louis, MO). Cells were treated with DMSO (vehicle control), 5 µM DMNQ, 1 or 10 µg/ml of cycloheximide, or DMNQ plus cycloheximide for 6 h. We conclude that the superinduction of GGT by cycloheximide in ajplung.physiology.org /cgi/content/full/274/3/L330   (4425 words)

Activation of Interferon Response Factor-3 in Human Cells Infected with Herpes Simplex Virus Type 1 or Human ... Monolayers were mock infected (M) or infected with 5 PFU of HSV-1 (strain 17) or 1 PFU of HCMV per cell in the presence or absence of 100 µg of cycloheximide (CH) per ml. RNA was extracted and analyzed by hybridization to ISG54- and GAPDH-specific probes, and the medium from the monolayers was centrifuged to remove virus. BJ cell monolayers were infected with 5 PFU of HSV-1 (strain 17) per cell or mock infected in the presence of 1 µg of actinomycin D (AMD) or 100 µg of cycloheximide (CH) per ml. jvi.asm.org /cgi/content/full/75/19/8909   (6694 words)

Glioma Cells Deficient in Urokinase Plaminogen Activator Receptor Expression Are Susceptible to Tumor Necrosis ... Cells were stained with propidium iodide after treatment with TRAIL, cycloheximide, or both and subjected to FACS analysis of apoptosis. Cycloheximide (cyc) was used at 1 µg/ml, and TRAIL was used at 20 ng/ml. Treatment with cycloheximide inhibited JNK activity in parental and vector cells but not in antisense uPAR cells. clincancerres.aacrjournals.org /cgi/content/full/7/12/4195   (3848 words)

Evidence for Fungal Dominance of Denitrification and Codenitrification in a Grassland Soil -- Laughlin and Stevens 66 ... was 58% due to cycloheximide and 7% due to streptomycin (Fig. cycloheximide was 65%, so 35% of the respiration was due to Confirmation of the efficacy of cycloheximide in this soil was soil.scijournals.org /cgi/content/full/66/5/1540   (4551 words)

Rapid turnover of GATA-2 via ubiquitin-proteasome protein degradation pathway -- Minegishi et al. 10 (7): 693 -- Genes ... After incubation with the medium with or without interleukin-3 for 20 h, cytokine-dependent Ba/F3 cells were treated with cycloheximide and analyzed as described in (A). Cells were treated with or without MG132 for 1 h and then treated with cycloheximide (10 µg/mL) or 30 J UV-C for 4 h. DMSO was used as a vehicle for cycloheximide and MG132. www.genestocellsonline.org /cgi/content/full/10/7/693   (4687 words)

Resistance to Fas-mediated apoptosis in human lung fibroblast -- Tanaka et al. 20 (2): 359 -- European Respiratory ... 4.— Apoptosis of WI-38 cells induced by CH-11 with cycloheximide stained with phycoerythrin-labelled Apo2.7 (a, c and e) or propidium iodide (PI) (b, d and f). The precursor form of procaspase-3, -8, and -9 in cell lysates of untreated cells (lane 1), cells at 48 h after cycloheximide alone (lane 2), CH-11 alone (lane 3), or CH-11 with cycloheximide administration (lane 4). 8.— Apoptosis of primary lung fibroblasts induced by CH-11 and cycloheximide, stained with Annexin-V fluorescein isothiocyanate. erj.ersjournals.com /cgi/content/full/20/2/359   (4134 words)

Yeast Cycloheximide-resistant crl Mutants Are Proteasome Mutants Defective in Protein Degradation -- Gerlinger et al. 8 ... Cycloheximide is a potent inhibitor of the ribosome. Data presented in this work show that cycloheximide resistance depends on defects in protein degradation. of cycloheximide but not when applying other kinds of inhibitors. www.molbiolcell.org /cgi/content/full/8/12/2487   (6295 words)

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