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Topic: DAPI

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	Drug and Alcohol Progam (Site not responding. Last check: 2007-10-28)
		In 1995, a Coast Guard Drug and Alcohol Program Inspector (DAPI) position was established in each of the nine Coast Guard Districts in an effort to increase compliance among the marine industry in respect to the chemical testing regulations.
		These DAPI positions were established in the geographical center of the applicable vessel distribution in the District.
		Although it is unrealistic to expect a DAPI to visit each and every marine employer within his or her district, they are expected to respond to any questions or problems you may have regarding your chemical testing program.
	www.uscg.mil /hq/gm/moa/dapihis.htm (4019 words)

	DAPI - Wikipedia, the free encyclopedia
		DAPI or 4',6-diamidino-2-phenylindole is a fluorescent stain that binds strongly to DNA.
		DAPI's blue emission is convenient for microscopists who wish to use multiple fluorescent stains in a single sample.
		Because DAPI readily enters live cells and binds tightly to DNA, it is toxic and mutagenic.
	en.wikipedia.org /wiki/DAPI (240 words)

	DAPI (Site not responding. Last check: 2007-10-28)
		The chemical structure of 4',6-Diamidino-2-phenylindole (DAPI), dimethylsulfoxide is shown at the right.
		This is a graph of the molar extinction coefficient of 4',6-Diamidino-2-phenylindole (DAPI) dissolved in dimethylsulfoxide.
		This is the fluorescence emission spectrum of 4',6-Diamidino-2-phenylindole (DAPI) dissolved in dimethylsulfoxide.
	omlc.ogi.edu /spectra/PhotochemCAD/html/dapi(DMSO).html (193 words)

	Nikon MicroscopyU: Fluorescence Microscopy - Triple Band Excitation Filter Combinations - DAPI-FITC-Texas Red
		DAPI (4',6-diamidino-2-phenylindole) is an ultraviolet light-absorbing dye with an absorption maximum at 358 and an emission maximum in the blue region at 461 nanometers.
		In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and Alexa Fluor 568 conjugated to phalloidin (targeting the cytoskeletal actin network; orange-red emission).
		Rabbit Bone Marrow Tissue with DAPI, Oregon Green 488, and Alexa Fluor 546 - A thin section of rabbit bone marrow tissue was stained for DNA and glycoproteins with DAPI and Oregon Green 488 conjugated to wheat germ agglutinin, respectively.
	www.microscopyu.com /articles/fluorescence/filtercubes/triple/dapifitctexasred/dapifitctexasredindex.html (2180 words)

	Nikon MicroscopyU: Fluorescence Microscopy - Triple Band Excitation Filter Combinations - DAPI-FITC-TRITC
		This filter set is designed for optimal detection of DAPI, FITC (fluorescein isothiocyanate), and TRITC (tetramethylrhodamine isothiocyanate) probes when applied in combination, and incorporates an excitation filter with narrow bandpass windows in the violet (385 to 400 nanometers), blue (475 to 490 nanometers), and green (545 to 565 nanometers) spectral regions.
		The ultraviolet light absorption maximum of DAPI is 358 nanometers and the visible emission maximum occurs at 461 nanometers, while the corresponding values for TMR are 550 and 573 nanometers.
		Canine Kidney Cells with DAPI, Alexa Fluor 568, and FITC - Examine the fluorescence emission intensity from a culture of canine kidney epithelial cells (Madin-Darby; MDCK line) that were immunofluorescently labeled with primary anti-human golgin-97 mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein (through the isothiocyanate, FITC).
	www.microscopyu.com /articles/fluorescence/filtercubes/triple/dapifitctritc/dapifitctritcindex.html (2132 words)

	Selective Nucleosome Disruption by Drugs That Bind in the Minor Groove of DNA -- Fitzgerald and Anderson 274 (38): ...
		DAPI (lanes 1-4, respectively) prior to addition of 300 ng of erthyrocyte nucleosomes.
		DAPI were subjected to reconstitution and gel analysis procedures as described in the legend to Fig.
		NaCl in the presence and absence of DAPI for 1 h at
	www.jbc.org /cgi/content/full/274/38/27128 (7969 words)

	FISH guide - washes, antifade, DAPI
		The solution should be stored in small aliquots (for 20-30 slides) at -20° C or -70° C. The vials should be kept as little as possible at room temperature (enough to get the required amount for the slides) and should be stored back in the freezer.
		Although both methods work, the quality of the banding pattern (DAPI) and the overall quality of the staining (contrast, brightness) is higher when the counterstain is added separately from the antifade.
		As general guidelines, DAPI and propidium iodide staining solutions are used at a concentration of 200 ng/ml DAPI or PI in 2xSSC or PBS.
	info.med.yale.edu /genetics/ward/tavi/fi07.html (1059 words)

	Invitrogen - Molecular Probes - Section 8.1 - Nucleic Acid Stains
		DAPI is thought to employ an intercalating binding mode with RNA that is AU selective.
		A review by Kapuscinski discusses the mechanisms of DAPI binding to nucleic acids, its spectral properties and its uses in flow cytometry and for chromosome staining.
		DAPI is quite soluble in water but has limited solubility in phosphate-buffered saline.
	www.probes.com /handbook/sections/0801.html (7159 words)

	Examples of CCK analysis
		As AMCA and DAPI spectra overlap, simultaneous DAPI counterstaining is not possible.
		Arrows in d indicate the chromosome translocations magnified in image f, in which the reverse DAPI staining is also shown.The colored dots and the corresponding numbers show the 3+3 color chart, which depicts the expected color of every chromosome pair in the first and second image, based on the colors assigned to each channel.
		When the green, red and blue channels were combined, the DAPI channel was shown only at a reduced lightness, in order to avoid interference with the red and green hybridization colors.
	info.med.yale.edu /genetics/ward/tavi/CCKexample.html (1185 words)

	Directory API - Starting and Ending a DAPI Session
		The first one initializes a DAPI session and the last one terminates it.
		However, I was able to use DAPI without it.
		One thing that you should consider when deciding whether to use DAPI or not is the performance drawback of the DAPIStart function.
	www.imibo.com /imidev/exc/dapi/dapistart.htm (1050 words)

	DAPI staining (Site not responding. Last check: 2007-10-28)
		4'-6-Diamidino-2-phenylindole (DAPI) is known to form fluorescent complexes with natural double-stranded DNA, showing a fluorescence specificity for AT, AU and IC clusters.
		Because of this property DAPI is a useful tool in various cytochemical investigations.
		When DAPI binds to DNA, its fluorescence is strongly enhanced, what has been interpreted in terms of a highly energetic and intercalative type of interaction, but there is also evidence that DAPI binds to the minor groove, stabilized by hydrogen bonds beween DAPI and acceptor groups of AT, AU and IC base pairs.
	www.celldeath.de /apometh/dapi.html (229 words)

	Thimerosal Induces DNA Breaks, Caspase-3 Activation, Membrane Damage, and Cell Death in Cultured Human Neurons and ...
		DAPI is a nonintercalating DNA-specific dye with an emission
		Note that the DAPI exclusion test reveals the early appearance of cells with compromised membranes and a stronger DAPI signal compared to the caspase-3 signal.
		Cell were fixed and stained by DAPI (1 µg/ml) to visualize nuclear morphology and apoptotic bodies.
	toxsci.oxfordjournals.org /cgi/content/full/74/2/361 (4672 words)

	Delaware Adolescent Program, Inc. Programs (Site not responding. Last check: 2007-10-28)
		The Delaware Adolescent Program, Inc. (DAPI) is the only statewide comprehensive school based program in the nation that serves pregnant and parenting teens and their families.
		DAPI serves the community with the following programs: Academic Instruction, Social Services, Child Care, and Medical Services.
		Certified teachers are provided to the pregnant and parenting teens served at the Delaware Adolescent Program, Inc. (DAPI).
	www.dapi.org /programs.htm (342 words)

	Forsburg Lab pombe pages: yeast pictures
		Wild type and mutant diploids, stained with DAPI and backlit by phase.
		Homothallic cells (h90) that have undergone mating, stained with DAPI and backlit by phase.
		These DAPI stained cells clearly show the difference between an interphase nucleus (with the "bite" of the nucleolus taken out) and the condensed mitotic nuclei.
	www-rcf.usc.edu /~forsburg/pictures.html (222 words)

	Unambiguous Identification of Implanted Cells After Cellular Cardiomyoplasty: A Critical Issue * Response -- Borenstein ...
		We conclude that re-uptake of DAPI disqualifies the use of this
		of DAPI by dead cells and its re-uptake by live cells is a minor
		DAPI labeling is actually used for tracking grafted cells and
	circ.ahajournals.org /cgi/content/full/109/18/e209 (928 words)

	Olympus Microscopy Resource Center: Fluorescence Microscopy Image Gallery - 3T3 Fibroblasts
		Nuclei of 3T3 cells grown in culture were stained with with the fluorophore DAPI and imaged utilizing a combination of fluorescence and phase contrast illumination.
		Incident light fluorescence microscopy is growing rapidly in importance as an investigational tool in the fields of medical and biological research.
		The specimen is a semi-confluent layer of 3T3 cells in tissue culture that were fixed and stained using DAPI.
	www.olympusmicro.com /galleries/fluorescence/pages/3t3dapismall.html (252 words)

	Portland/DAPI IRC meeting \| www.kdedevelopers.org (Site not responding. Last check: 2007-10-28)
		We had an IRC meeting about Portland's DAPI today and while most people seemed to have joined to discuss API related things, it more or less turned into a session to clarify positioning in relation with other projects cleaning up some misconceptions.
		Currently there is only one single file, covering all available methods of DAPI at this point.
		There were a couple of people worried that DAPI would require a "man in the middle" even when all functionality would be available through the environments already running processes.
	www.kdedevelopers.org /node/2550 (1040 words)

	DAPI (Site not responding. Last check: 2007-10-28)
		The chemical structure of 4',6-Diamidino-2-phenylindole (DAPI) is shown at the right.
		This is a graph of the molar extinction coefficient of 4',6-Diamidino-2-phenylindole (DAPI) dissolved in water.
		This is the fluorescence emission spectrum of 4',6-Diamidino-2-phenylindole (DAPI) dissolved in water.
	omlc.ogi.edu /spectra/PhotochemCAD/html/dapi(H2O).html (204 words)

	Instructions (Site not responding. Last check: 2007-10-28)
		For the Fifth District, DAPI functions and program oversight responsibility reside within the Marine Safety Division.
		The intent of the chemical testing regulations is to provide a means to minimize the use of intoxicants by merchant marine personnel and to promote a drug free and safe work environment.
		Provide information to the D5 DAPI regarding meetings, seminars and events of interest and coordinate D5 DAPI attendance and participation if needed.
	www.uscg.mil /d5/msafety/dapiweb/instructions.htm (959 words)

	DAPI (Site not responding. Last check: 2007-10-28)
		Dapi is used to stain the DNA in nuclei, fluorescent, and have seen it used on both fixed (formalin and acetone) tissues, cells and bacteria.
		You can actually buy a mounting media containing DAPI, a nice way to counterstain the nuclei a bluish fluorescence with another immunofluorescent label, Vectashield with DAPI from Vector.
		Biotechnics and Histochemistry journal has had several articles on DAPI, Vector@vectorlabs.com (ask for Craig) may also have references concerning the use of DAPI.
	www.histosearch.com /histonet/Jul00A/DAPIA.html (118 words)

	The Use of DAPI As a Replacement for Ethidium Bromide in Forensic DNA Analysis (Site not responding. Last check: 2007-10-28)
		,6-diamidino-2-phenylindole dihydrochloride (DAPI), can be used in place of ethidium bromide for yield and restriction gels, and to stain the visual marker of analytical gels.
		A low concentration of DAPI is added directly to the DNA.
		Most of the stain is immediately bound to the DNA and remains bound during electrophoresis.
	www.astm.org /JOURNALS/FORENSIC/PAGES/207.htm (283 words)

	D8 DAPI (via CobWeb/3.1 planetlab1.cs.wisc.edu) (Site not responding. Last check: 2007-10-28)
		The District DAPI is responsible for the implementation of the Department of Transportations and the United Stated Coast Guard drug and alcohol testing regulations.
		The DAPI for District Eight is located at the District Office in New Orleans.
		The DAPI works as a liaison between all District units, Coast Guard Headquarters, the Drug Testing Industry, and the Marine Community to ensure that all areas of the drug testing program are adequately met.
	www.uscg.mil.cob-web.org:8888 /d8/Divs/M/DAPI.htm (134 words)

	Chemicon - Product #S7113 - DAPI/Antifade Solution, Ready to Use
		Liquid PBS pH 8.0 containing ~50% glycerol and 0.4μg/mL of DAPI and 2-7mM phenylenediamine.
		Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.
		DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987).
	www.chemicon.com /browse/productdetail.asp?ProductID=S7113 (214 words)

	U3 Developer Blog » Blog Archive » U3 Smart vs U3 Aware: Where to go with the DAPI? (via CobWeb/3.1 ... (Site not responding. Last check: 2007-10-28)
		U3 aware application is any application, U3 smart or otherwise, that uses the U3 Device API (DAPI) to monitor device connect and disconnect events, perform discovery and control operations on a U3 smart drive that is connected to the host on which the application is running.
		We need to hear from software developers where they think we should be extending the DAPI so that innovative third party solutions are enabled.
		This entry was posted on Monday, August 7th, 2006 at 11:14 am and is filed under Technical Resources.
	devblog.u3.com.cob-web.org:8888 /?p=21 (453 words)

	DAPI/Distamycin A Staining (Site not responding. Last check: 2007-10-28)
		Distamycin A tends to lack stability in aqueous solution unless it is frozen.
		DAPI stock solution can be frozen or refrigerated for several weeks without detectable deterioration.
		The stained preparations tend to fade rapidly, so photography should be immediate.
	homepage.mac.com /wildlifeweb/cyto/text/DAPI.html (346 words)

	Rapid DAPI Screen (Site not responding. Last check: 2007-10-28)
		A rapid screen for chromosome missegregation mutants by DAPI staining
		Put water in the wells using a squashing bottle gently.
		Turn upside down, put on the stage and examine using a lens for long distance focus.
	www.ciwemb.edu /labs/koshland/Protocols/MICROSCOPY/missegscreen.html (128 words)

	kbAlertz: (165931) - Creating a custom recipient programmatically requires the use of the DAPI functions. Using ... (Site not responding. Last check: 2007-10-28)
		Using DAPIStart to create a DAPI session is the first step to making this work.
		Creating a custom recipient programmatically requires the use of the DAPI functions.
		Create a DAPI session using the DAPIStart function.
	www.kbalertz.com /kb_165931.aspx (700 words)

	DAPI Staining Acetabularia (Site not responding. Last check: 2007-10-28)
		Place the cells in the dark at room temperature and allow to incubae for 4 hours.
		Use the dibasic to adjust the pH of the monobasic.
		NOTE: DAPI stock solution, IFS with DAPI, and cells that have been stained with DAPI must be refrigerated in the dark.
	faculty.washington.edu /mandoli/methods/genetics/Adapi.html (268 words)

	wrconsulting.com
		I have shown how attributes of a MS Exchange Directory item may be retrieved with DAPI functions, and dumped to the screen.
		Among other things I initialize the base point in the directory tree and a container (these two members tell DAPI where to look for entries).
		Note that base point in your case will be different because it is a distinguished name containing organization and site names.
	www.wrconsulting.com /Software/Publications/Exchange/Dir/DAPIWrite.htm (789 words)

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