DNA DNA hybridisation

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Topic: DNA DNA hybridisation

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	DNA-DNA hybridisation - Wikipedia, the free encyclopedia
		DNA-DNA hybridization is a method in genetics to measure the degree of genetic similarity between DNA sequences.
		The technique is usually used to determine the genetic "distance" between two species.
	en.wikipedia.org /wiki/DNA-DNA_hybridisation (264 words)

	Natural History Collections: DNA-DNA Hybridisation
		The technique of DNA-DNA hybridisation measures the degree of similarity between the genomes of different species, and therefore predicts genealogies of extant species by comparing the genetic resemblance between lineages that diverged millions of years ago.
		As a rule of thumb, a difference in melting temperature of one degree is equivalent to a difference in DNA sequence of one percent, (i.e, one nucleotide in a sequence of one hundred) (Lewin 1997).
		The extent to which this occurs is measured by gradually heating the 'hybrid DNA' formed by the association of the two species, until the hydrogen bonds between the strands become so strained that they break, or melt, yielding single strands once more.
	www.nhc.ed.ac.uk /index.php?page=236.438.446 (414 words)

	Sibley-Monroe checklist 18 - Wikipedia, the free encyclopedia
		It drew on extensive DNA-DNA hybridisation studies to reassess the relationships between modern birds.
		The Sibley-Monroe checklist was a landmark document in the study of birds.
	en.wikipedia.org /wiki/Sibley-Monroe_checklist_18 (34 words)

	Comparison of 16S rDNA-based PCR and checkerboard DNA-DNA hybridisation for detection of selected endodontic pathogens -- SIQUEIRA et al. 51 (12): 1090 -- Journal of Medical Microbiology
		gingivalis DNA; 4, A. actinomycetemcomitans DNA; 5, Por.
		Non-isotopic DNA probes for the identification of subgingival microorganisms.
		DNA probe and enzyme analysis in periodontal diagnostics.
	jmm.sgmjournals.org /cgi/content/full/51/12/1090 (3257 words)

	BioMed Central Full text Inter-familial relationships of the shorebirds (Aves: Charadriiformes) based on nuclear DNA sequence data
		The DNA sequence data suggests that the order Charadriiformes is monophyletic, and that the sandgrouses and bustards are not part of it.
		Johnson KP, Clayton DH: A molecular phylogeny of the dove genus Zenaida: Mitochondrial and nuclear DNA sequences.
		As with many other systematic relationships supported by the DNA sequence data analysed herein, the grouping of the thick-knee with the sheathbill is unexpected when morphology and general behaviour are taken into consideration.
	www.biomedcentral.com /1471-2148/3/16 (5971 words)

	ScienceDaily: Dna dna hybridisation
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	www.sciencedaily.com /encyclopedia/dna_dna_hybridisation (905 words)

	Natural History Collections: DNA Sequencing
		The use of DNA sequencing has rendered distance measures such as immunodiffusion and DNA-DNA hybridisation largely obsolete, as these older methods cannot provide the detailed character state information of DNA sequences.
		Having been cloned, the DNA is sequenced, revealing the chain of nucleotides that constitute its code (Paterson 1979).
		When first developed, DNA sequencing depended on cloning - inserting the portion of DNA to be studied into a bacterial virus, which then replicated the DNA fragments.
	www.nhc.ed.ac.uk /index.php?page=236.438.448 (215 words)

	HSC Online
		By comparing the mitochondrial DNA from living primates it is possible to calculate a molecular clock based on a constant rate of mutation.
		In DNA sequencing genetic and biochemical tests are used to sequence the bases in a portion of DNA.
		Most of the DNA in a cell is found in the nucleus but a small amount of DNA is found in the mitochondria.
	hsc.csu.edu.au /biology/options/human_story/2938/Bio982_18Mar04.html (2297 words)

	7luku.html
		Dot-blot DNA-DNA hybridisation was found to be a good screening method that could be used for the selection of strains for quantitative DNA-DNA reassociation studies.
		For routine purposes we strongly recommend the use of highly standardised SDS-PAGE protein profile analysis for identification of culturable "gastrospirilla", since the results obtained were in excellent agreement with both the dot-blot, and quantitative DNA-DNA hybridisation analyses.
		Its application to the taxonomy of these bacteria has been confusing and the true genetic relationship between cultured and uncultured animal and human "gastrospirilla" remains to be solved by more discriminative methods such as DNA-DNA hybridisation.
	ethesis.helsinki.fi /julkaisut/ela/elint/vk/jalava/7luku.html (520 words)

	Irestedt, M
		The agreement between data from the nuclear DNA and other sources, along with the fact that neither of the studied genes showed sign of saturation, indicate the great potential of these two nuclear genes to resolve very old divergences in birds.
		Nuclear DNA data also unambiguously group the lyrebirds Menura with the oscines.
		Irestedt, M., Johansson, U. S., Parsons, T. and Ericson, P. Phylogeny of major lineages of suboscines (Passeriformes) analysed by nuclear DNA sequence data.
	www.oikos.ekol.lu.se /Jab.32.1.abstract/2611.html (286 words)

	Science Museum Your genes DNA hybridisation
		DNA has a unique quality that all DNA experiments rely on - its natural ability to stick to a 'complementary copy' of itself, a process called DNA hybridisation.
		The chemical letter A will always pair up with a T, and a C with a G. DNA hybridisation.
	www.sciencemuseum.org.uk /exhibitions/genes/139.asp (55 words)

	B200 Human Genome Lectures
		However, they are still based around the techniques which we have studied in B200, DNA-DNA hybridisation, the Polymerase Chain Reaction and the dideoxy chain termination method of DNA sequencing.
		By asking in which hybrid lines is a DNA locus present, (by PCR or by Southern blotting) and by correlating this with the karyotypes of the hybrid lines it is possible to deduce mapping information.
		Metaphase chromosome spreads are made by conventional techniques, the spread chromosomes are treated to denature the DNA of the chromosomes and the probe is allowed to hybridise to the chromosomes.
	www.ucl.ac.uk /~ucbhjow/b200/b200.html (2368 words)

	eLookup Encyclopedia
		DNA DNA-DNA_hybridisation DNA_chip DNA_computing DNA_electrophoresis DNA_fingerprint DNA_fingerprinting DNA_ladder DNA_ligase DNA_microarray DNA_polymerase DNA_replication DNA_sequencer DNA_test DNA_testing DNA_topology DNA_vaccination DNA_virus DNF DNIX DNR DNS DNS/BIND DNS/MaraDNS DNS/djbdns DNSBL DNS_Server Dna
		The text of this article is licensed under the GFDL.
	www.ebroadcast.com.au /lookup/encyclopedia/dn (48 words)

	DNA hybridisation - OneLook Dictionary Search
		DNA hybridisation : The On-line Medical Dictionary [home, info]
		Tip: Click on the first link on a line below to go directly to a page where "DNA hybridisation" is defined.
		We found one dictionary with English definitions that includes the word DNA hybridisation:
	www.onelook.com /?w=DNA+hybridisation&ls=all (71 words)

	M003: MOLECULAR EPIDEMIOLOGY OF BURKHOLDERIA (PSEUDOMONAS) CEPACIA .
		Identification of the isolates at the species level was only obtained by numerical analysis of SDS-PAGE electrophoregrams and DNA:DNA hybridisation.
		Any colony resembling B.cepacia was fully identified by traditional biochemical tests, the API-ZYM system, gas-liquid chromatography of bacterial fatty acid methyl esters (FAME), SDS-polyacrylamide gel electrophoresis of bacterial proteins (SDS-PAGE) and, for some strains, DNA:DNA hybridisation.
		The results of this comparative study showed that the commercial B.cepacia Selectatab medium was the best selective medium for growing B.cepacia.
	www.ecfsoc.org /brussels/microbiol/m00395.html (1076 words)

	DSMZ
		DNA - DNA hybridisation per pair of strains
		Preparation of DNA for DNA - DNA hybridization
		Full phylogenetic study by complete 16S rDNA sequence analysis
	www.dsmz.de /identification/main.php?contentleft_id=5 (36 words)

	Babbler - Art History Online Reference and Guide
		Prior to the development of modern molecular research methods (notably DNA-DNA hybridisation) the members of the Pomatostomidae were included in the Timaliidae.
	www.arthistoryclub.com /art_history/Babbler (74 words)

	Misidentification of a genomovar of Burkholderia cepacia by recA restriction fragment length polymorphism -- Moore et al. 55 (4): 309 -- Journal of Clinical Pathology
		DNA–DNA hybridisation to classify them as a separate species.
		This is mainly the result of (1) poor quality genomic DNA for
		on visible differences in DNA banding patterns on agarose gels.
	jcp.bmjjournals.com /cgi/content/full/55/4/309 (1678 words)

	MPP: A microarray-to-phylogeny pipeline for gene content analysis
		MPP is a microarray analysis tool, providing a novel approach to infer phylogeny directly from raw DNA:DNA hybridisation microarray data.
		Three steps control the flow of data through the system: (a) ExcelParser / CSVParser - these classes process Microsoft Excel (.xls) and flat file text (.csv /.gpr) data respectively.
	www.iscb.org /ismb2004/posters/robert.daveyATbbsrc.ac.uk_168.html (231 words)

	Lund University, Doctoral Dissertation
		Comparison of the 16S rDNA sequence showed that the organism was phylogenetically closely related to Nesterenkonia species, although differences in G+C content and low DNA-DNA hybridisation demonstrated that the organism represents a new species, which was named Nesterenkonia abyssinica.
		The alkaline protease was produced by the organism in alkaline medium (pH 10), using feather as the only sole source of carbon and nitrogen.
		The organism grew at 37°C, pH 10, and 1M NaCl.
	www.lub.lu.se /cgi-bin/show_diss.pl/tec_821.html (589 words)

	Partnership for Action Against Wildlife Crime (PAW) - Publications Defra
		Methods: PCR; DNA DNA hybridisation; DNA RNA hybridisation; Western blotting.
		Methods: Microsatellite and mitochondrial DNA analysis for species and individual recognition, including PCR amplification of trace quantities, primer development, sequencing and assignment of parentage.
		Blood, Ussue and DNA bank for the Zoological Society of London.
	www.defra.gov.uk /paw/publications/dnadir/dir_i.htm (393 words)

	CSIRO PUBLISHING - Australian Journal of Zoology
		DNA/DNA hybridisation analysis was undertaken to resolve the phylogenetic relationships within the chromosomally diverse genus Petrogale.
		Phylogeny of the rock-wallabies, Petrogale (Marsupialia : Macropodidae) based on DNA/DNA hybridisation
	www.publish.csiro.au /nid/90/paper/ZO01034.htm (186 words)

	pelmat.txt
		DNA-DNA Hybridisation Matrices - PHYLIP format Abbreviations & Expansions: van Tuinen et al.
	www.evogenomics.org /publications/data/flamingo/pelmat.txt (13 words)

	The Centre for BIOARRAY Innovation: Projects
		Developing electrochemical DNA hybridisation sensors, enzyme-linked immunoassay-based micro-electrode array sensors and the development of arrays with individually addressable groupings of micro-electrodes.
		Demonstrating that microarrays of oligonucleotide probes, suitable for use as DNA chips, can be spontaneously and reproducibly assembled from colloidal particles coated with oligonucleotide probes.
		Developing a method for quantitative DNA/RNA analysis based on gold particle aggregation, and integrating gold nanoparticle DNA analysis on a chip.
	www.postgenomeconsortium.com /bioarray/projects.html (675 words)

	DNA-DNA hybridisation -- Facts, Info, and Encyclopedia article
		The single-stranded DNA is now allowed to anneal with the DNA pieces of the other species.
		The DNA double strand is then separated by heating into two single strands.
		These results in a profile from which the amount of similar DNA, and thus the degree of genetic similarity, can be determined.
	www.absoluteastronomy.com /encyclopedia/d/dn/dna-dna_hybridisation5.htm (369 words)

	ENS microarray platform: DNA microarray principle
		Prior to hybridisation, DNA is denatured to obtained a single strand DNA on the microarray, this will allow the probe to bind to the complementary strand from the target.
		DNA microarrays allow for rapid measurement and visualisation of differential expression between genes at the whole genome scale.
		DNA fragments amplified by PCR technique are spotted on a microscopic glass slide coated with polylysine prior to spotting process.
	www.transcriptome.ens.fr /sgdb/presentation/principle.php (585 words)

	article.html
		Hybridisation of DNA, or formation of a DNA double helix by mutual recognition of two single_stranded DNA strands that have complementary sequences to one another.
		During the early development of DNA sequence analysis, genomic DNA was used as a species-specific probe and was hybridised to DNA extracted from meat samples [1, 7, 9, 26].
		DNA can be extracted from meat samples by using one of three methods: rapid alkaline lysis (Protocol 1), a similar procedure to Protocol 1 but with extra purification steps (Protocol 2), the common proteinase K/sodium dodecyl sulphate (SDS) procedure (Protocol 4).
	www.vetscite.org /publish/articles/000012/article.html (4187 words)

	PAG-XII: (P79) GENOME-SPECIFIC DNA FRAGMENTATION AND ELIMINATION DURING EARLY EMBRYOGENSIS AFTER WHEAT AND PEARL MILLET CROSSING
		Insight into the mechanisms underlying the selective elimination of pearl millet DNA was obtained by combination of in situ hybridisation and terminal deoxyribonucleotidyl transferase (TdT)-mediated dUTP-fluorescein nick end-labelling (TUNEL) method on developing embryos of wheat and pearl millet crosses.
		However, we have first evidence that uniparental DNA can also be removed from the nucleus by a novel micronucleation mechanism that is initiated by genome-specific fragmentation of DNA during the interphase.
		Given the complexity of the eukaryotic genome the ability of the cell to cleave pearl millet-DNA with specificity and to discriminate it from the wheat genome is unlikely.
	www.intl-pag.org /12/abstracts/P04_PAG12_79.html (4187 words)

	Applied Molecular Biology
		DNA-structure/ genomic sequencing/ DNA methylation/ DNA-protein interaction/ PCR/ yeast two-hybrid system/ site-directed mutagenesis/ differential display/ DNA arrays/ in situ hybridization and PCR/ FISH/ cDNA libraries/ RACE/ mega-molecular biology/ PFGE/ genome projects/ biocomputing/ GCG software /molecular biology on the internet
		In this interfaculty lecture course, key methods in molecular biology will be discussed, including relevant background information and current protocols.
		The lectures will be in English; dicussion and questions in German are possible.
	www.cx.unibe.ch /ikp/angewmolbio.html (4187 words)

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Topic: DNA DNA hybridisation

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