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Topic: DNA construct

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DNA sequences

mitochondrial DNA

DNA DNA hybridisation

DNA polymerases

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junk DNA

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Noncoding DNA

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	[No title] (Site not responding. Last check: 2007-10-29)
		A recombinant DNA construct according to claim 9 wherein the construct is capable of being expressed in the tissue or tissues of a germinating seedling or a developing grain or a plant when the construct is integrated within the grain's or seedling's or plant's genomic DNA.
		A recombinant DNA construct according to claim 21 wherein the construct is capable of being expressed in the tissue or tissues of a germinating seedling or a developing grain or a plant when the construct is within the grain's or seedling's or plant's genomic DNA.
		A recombinant DNA construct according to claim 32 wherein the construct is capable of being expressed in the tissue or tissues of a germinating seedling or a developing grain or a plant when the construct is integrated within the grain's or seedling's or plant's genomic DNA.
	www.mindfully.org /GE/GE4/US-Patent-6228643.txt (14262 words)

	CIPO - Canadian Patent Database - Claims - 2281002
		A DNA construct according to claim 1, wherein said DNA construct comprises a plurality of trait DNA molecules each having a length that is insufficient to impart that trait to plants transformed with that trait DNA molecule.
		A DNA construct according to claim 2, wherein the trait DNA is a viral cDNA molecule and the trait is viral disease resistance.
		A DNA expression vector according to claim 23, wherein said DNA construct comprises a plurality of trait DNA molecules each having a length that is insufficient to impart the trait to plants transformed with that trait DNA molecule.
	patents1.ic.gc.ca /claims?patent_number=2281002&language=EN_CA (2527 words)

	DNA analysis - Hutchinson encyclopedia article about DNA analysis
		It determines the pattern of certain parts of the genetic material DNA that is unique to each individual.
		The world's first national DNA database began operating in the UK in April 1995 in accordance with the 1994 Criminal Justice and Public Order Act, which states that those convicted or suspected of sex offences, serious assaults, or burglaries in England and Wales must provide a sample of saliva or hair for DNA analysis.
		In the UK, DNA testing was used nationally for the first time in 1996, involving the testing of 1,200 persons, in a police hunt for a murderer.
	encyclopedia.farlex.com /DNA+analysis (426 words)

	United States Patent: 5,280,112 (Site not responding. Last check: 2007-10-29)
		An isolated DNA molecular having a nucleotide sequence encoding a bovine adrenotropic hormone receptor wherein the DNA sequence is substantially homologous to the sequence in FIGS.
		A recombinant expression construct comprising the DNA sequence of claim 1, wherein the construct is capable of expressing the bovine adrenocorticotropic hormone receptor in a transformed eukaryotic cell culture.
		DNA which encodes the ACTH.sup.R may be obtained, in view of the instant disclosure, by chemical synthesis, by screening reverse transcripts of mRNA from appropriate cells or cell line cultures, by screening genomic libraries from appropriate cells, or by combinations of these procedures, as illustrated below.
	www.webpatent.com /patents/p5280112.htm (5499 words)

	VIDO - Solutions Through Research
		A DNA construct comprising a first nucleotide sequence encoding interleukin-2 (IL-2), operably linked to a second nucleotide sequence encoding a leukotoxin, wherein said leukotoxin is characterized by having the amino acid sequence G-G-X-G-X-D, where X is K, D, V or N. The DNA construct of claim 1 wherein said IL-2 is bovine IL-2.
		In one embodiment, the present invention is directed to a DNA construct comprising a first nucleotide sequence encoding a cytokine, or an active fragment thereof, operably linked to a second nucleotide sequence encoding at least one epitope of leukotoxin.
		A DNA "coding sequence" or a "nucleotide sequence encoding" a particular protein, is a DNA sequence which is transcribed and translated into a polypeptide in vivo when placed under the control of appropriate regulatory sequences.
	www.vido.org /patents/patent.php?patentnumber=5,238,823 (9512 words)

	Fifth Foresight Conference: DNA Nanotechnology
		DNA is an extremely favorable construction medium: The sticky-ended association of DNA molecules occurs with high specificity, and it results in the formation of B-DNA, whose structure is well known.
		The molecule is constructed from three segments, a duplex consisting of strands L1 and L2, a duplex consisting of strands R1 and R2, and the tetramobile junction, consisting of strands JT and JB.
		[27] Du, S.M. and Seeman, N.C. (1994), The construction of a trefoil knot from a DNA branched junction motif, Biopolymers 34, 31-37.
	www.foresight.org /Conferences/MNT05/Papers/Seeman (9193 words)

	Homolgous Recombination & Knockout Mouse
		The next step is to design and fabricate the DNA construct you want to insert into the chromosome in place of the wild-type allele.
		This construct may contain any DNA sequence of your choosing which means you can insert different alleles (both functional and non-functional ones), different genes or reporter genes (e.g.
		The original engineered construct has taken up the targeted gene of interest but since it cannot replicate in a nucleus, it is lost quickly in dividing cells while the modified chromosome replicates faithfully, including its new insert.
	www.bio.davidson.edu /courses/genomics/method/homolrecomb.html (1036 words)

	[No title]
		In that article, Dr. Axel described a process called "co\_ transformation", where a mammalian cell is transformed with the gene of interest (introduced into the cell on one DNA construct), and, at the same tim e, is transformed with a second gene known as a "marker gene" (introduced on a second DNA construct).
		The claims also claimed co\_ transformation using a single DNA construct on which the marker gene and the gene of interest were linked prior to i ncorporation into the cell.
		To distinguish Axel, the applicants specifically argued that their invention involved linking of the beta interferon gene and the marker gene on a single DNA construct and that the Axel publi c ation described using two unlinked constructs, one carrying the interferon gene and one carrying the marker gene.
	www.law.harvard.edu /academics/registrar/exams_99-00/rtf/lee01.rtf (2160 words)

	Utilization of MHC Class I Transgenic Mice for Development of Minigene DNA Vaccines Encoding Multiple HLA-Restricted ...
		DNA immunization to that of the Theradigm-HBV lipopeptide, a vaccine
		Construction of multiepitope minigene and HBV Pol DNA plasmids
		DNA multi-CTL epitope vaccines for HIV and Plasmodium falciparum: immunogenicity in mice.
	www.jimmunol.org /cgi/content/full/162/7/3915 (7954 words)

	[No title]
		PI responsibilities: PI agrees to provide the DNA construct, cell lines, or mice, and as well as all disclosures and approvals required under this Agreement and by all UR regulatory requirements.
		DNA Construct or Cell Line Disclosure, Institutional Approvals, Restricted Genetic Materials & Condition of Genetic Materials A.
		PI hereby represents and warrants that the DNA construct(s) or cell line(s) will not produce any infectious condition that may be harmful to other animals, humans or the environment and that experimentation does not require containment conditions greater than those required under NIH RAC BL-2 standards.
	www.urmc.rochester.edu /research/transgenic/word/agreement.doc (568 words)

	Karolinska Center for Transgene Technologies
		For overexpression, a promoter/enhancer that directs expression to a particular organ or timepoint during development is coupled to the gene or cDNA of interest, and for gene regulation studies, the promoter/enhancer of interest is coupled to a reporter gene.
		A purified DNA construct is injected into fertilized mouse eggs at the one-cell stage.
		Cells that have integrated the introduced DNA in their genome are selected using a drug that kills cells which have not taken up the marker gene.
	www.kctt.ki.se /dok/flow.htm (558 words)

	Metastasis-inducing DNA Regulates the Expression of the Osteopontin Gene by Binding the Transcription Factor Tcf-4 -- ...
		construct and fragments of one metastasis-inducing DNA (Met-DNA)
		The relative CAT activity is the CAT activity with different DNAs and/or transfected cells relative to that with the promoter-reporter construct alone in Rama 37 cells, normalized as described in "Materials and Methods." Results are the mean ± SD of three separate experiments, but for clarity the minus SD bars have been omitted.
		the C9-Met-DNA fragments and the opn promoter-reporter construct
	cancerres.aacrjournals.org /cgi/content/full/61/14/5619 (8325 words)

	Function and Assembly of the Bacteriophage T4 DNA Replication Complex: INTERACTIONS OF THE T4 POLYMERASE WITH VARIOUS ...
		values for the binding of T4 DNA polymerase to various DNA p-t junction constructs that differ in the length of the single-stranded part, ranging from 1 nt (for the 15–16-mer construct) to 8 nt (for the 15–23-mer construct), as measured by steady-state fluorescence anisotropy at 20 °C in the same buffer as in Figs.
		the DNA polymerase to a p-t junction in the presence of the
		the affinity of the polymerase for the p-t junction DNA by
	www.jbc.org /cgi/content/full/278/28/25435 (9227 words)

	umtmcf (Site not responding. Last check: 2007-10-29)
		Transgenic mice are constructed by injecting cloned DNA into the pronucleus of fertilized mouse eggs; those eggs that survive the injection are implanted in foster females to develop to term.
		In particular, the purity of the DNA construct to be injected is very important to avoid toxic effects to the embryos, and the DNA must be at the proper concentration for injection.
		The DNA construct must be purified away from vector sequences.
	www.umanitoba.ca /faculties/medicine/physiology/transgenic/umtmcf.htm (625 words)

	Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates ...
		Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates disease after challenge with influenza A virus -- Heinen et al.
		The expression vector was constructed from pVR1012, a mammalian expression vector containing the HCMV immediate-early transcriptional enhancer, promoter and intron A regulatory elements and the poly(A) signal and transcription termination sequences of the bovine growth hormone in a pUC18 backbone.
		Construction, preparation and analysis of the M2eNP DNA construct.
	vir.sgmjournals.org /cgi/content/full/83/8/1851 (5142 words)

	NOVEL CHIMERIC DNA CONSTRUCT THAT ELICITS MHC CLASS II RESTRICTED CD4+ T CELL RESPONSES WITH POTENTIAL APPLICATIONS IN ... (Site not responding. Last check: 2007-10-29)
		In vivo Studies using ESAT-6 antigen from M. Tuberculosis ESAT-6, a secretory protein from M. tuberculosis that provides protection against challenge by the bacterium in mice at levels similar to the BCG vaccine, was fused to the different CD1 targeting sequences and the resultant DNA construct used as a vaccine.
		To further determine the biological relevance the resultant MART-1 specific responses induced through DNA vaccination with respect to tumor growth progression, 4-5 mice representing each experiment group were inoculated with B16 melanoma cells upon completion of the immunization protocol.
		Results indicate that tumor growth was slowest in mice immunized either with the fusion construct or the adenovirus transduced DC which exhibited similar capabilities.
	www.research.ucla.edu /tech/ucla02-146.htm (620 words)

	Ectopic Expression of DNA Encoding IFN-{alpha}1 in the Cornea Protects Mice from Herpes Simplex Virus Type 1-Induced ...
		DNA on the establishment of HSV-1 latency and reactivation,
		DNA immunization against herpes simplex virus: enhanced efficacy using a Sindbis virus-based vector.
		DNA from bacteria, but not vertebrates, induces interferons, activates natural killer cells, and inhibits tumor growth.
	www.jimmunol.org /cgi/content/full/162/7/4184 (4763 words)

	Gene-gun DNA vaccination aggravates respiratory syncytial virus-induced pneumonitis -- Bartholdy et al. 85 (10): 3017 ...
		DNA vaccination also led to enhanced disease during RSV challenge.
		DNA was coated on to 1·6 nm gold particles at a
		Pertmer, T. M., Roberts, T. and Haynes, J. Influenza virus nucleoprotein-specific immunoglobulin G subclass and cytokine responses elicited by DNA vaccination are dependent on the route of vector DNA delivery.
	vir.sgmjournals.org /cgi/content/full/85/10/3017 (4249 words)

	(WO 00/09708) DNA CONSTRUCTS COMPRISING PROTEASE ENCODING SEQUENCES OR INHIBITORS THEREOF (Site not responding. Last check: 2007-10-29)
		DNA CONSTRUCTS COMPRISING PROTEASE ENCODING SEQUENCES OR INHIBITORS THEREOF
		These constructs are preferably rendered reversible by the presence of further elements.
		They can be used in plant or mammalian cells for disruption of cell function, controlling senescence and modifying the metabolism of stored proteins.
	wipo.int /cgi-pct/guest/getbykey5?KEY=00/09708.000224&ELEMENT_SET=DECL (165 words)

	PG-IV: P1305 - A Multi-Functional T-DNA Construct for Transposon Mutagenesis and Plant Genome Analysis (Site not responding. Last check: 2007-10-29)
		A Multi-Functional T-DNA Construct for Transposon Mutagenesis and Plant Genome Analysis
		We introduce a new multifunctional T-DNA construct for transposon tagging and map-based cloning in the plant genome.
		Besides the in vivo induction of chromosomal rearrangements, the system provides a way to construct a physical map of the region around the T-DNA integration site and the possibility to clone the rearranged segment of genomic DNA by using in vitro site-specific manipulation of DNA.
	www.intl-pag.org /4/abstracts/p305.html (288 words)

	[No title] (Site not responding. Last check: 2007-10-29)
		DNA FRAGMENT INFORMATION: Investigator:___________________________________________ Construct Name:________________________________________ Total DNA fragment length:_______________________________ Fragment Purified by:____________________________________ DNA stock Concentration:_________________________________ DNA construct should be dissolved in 10mM Tris/0.25mM EDTA, PH 7.5 that has been filtered with a.02 mm filter.
		If you have followed guidelines we will do a second round of injections with a new prep of your construct at no charge.
		PCR assays should be done at least twice showing positive controls working before we do a second injection session. If the second sessions show no positives the construct should be re-evaluated and discussed.
	www.ahsc.arizona.edu /transgenicmouse/Application.doc (334 words)

	Large construct DNA modification (Site not responding. Last check: 2007-10-29)
		For example, you can make insertions of several kilobases to your clone and study how extra DNA modifies the pathway or the disease phenotype.
		Alternatively, you can delete fragments of DNA for transgenic analysis and discover the effects of loss of function in cells.
		With conventional PCR based site-directed- mutagenesis the whole vector construct including the target sequence has to be amplified by PCR which is not technically feasible.
	www.biost.com /DNA-modification.htm (420 words)

	Construct DNA plasmid for QPCR - BioForum
		Using DNA and RNA as quantitative standard make alot of different.
		Quantification of viral RNA using DNA as standard is not valid.
		Are you trying to determine the number of DNA intermediate or latent infaction of retrovirus.
	www.protocol-online.org /forums/index.php?showtopic=7075 (561 words)

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