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Topic: DNA holoenzyme

Related Topics

PolIII holoenzyme

DNA polymerase

DNA replication

DNA ligase

Nuclease

Phosphodiester bond

RNase H

Wills Point, Texas

Little Conemaugh River

Koryo, Shimane

BLT sandwich

Margaret Okayo

	DNA Synthesis
		Histone H1 occupies the internucleosomal DNA and is identified as the linker histone.
		DNA recombination refers to the phenomenon whereby two parental strands of DNA are spliced together resulting in an exchange of portions of their respective strands.
		DNA damage can occur as the result of exposure to environmental stimuli such as alkylating chemicals or ultraviolet or radioactive irradiation and free radicals generated spontaneously in the oxidizing environment of the cell.
	web.indstate.edu /thcme/mwking/dna.html (4122 words)

	Devoted to the lagging strand[mdash]the [chi] subunit of DNA polymerase III holoenzyme contacts SSB to promote ...
		DNA polymerase III holoenzyme (Pol III holoenzyme) is the multisubunit replicase of the Escherichia coli chromosome.
		SSB-113 is a conditional lethal mutant with a pleiotropic phenotype having defects in replication and recombination (Chase, 1984; Chase and Williams, 1986; Meyer and Laine, 1990; Lohman and Ferrari, 1994).
		In Figure 9A, the initiation complex of the holoenzyme with DNA was formed in a pre-incubation at low ionic strength, then NaCl was added and replication was initiated upon adding the remaining two dNTPs.
	www.nature.com /emboj/journal/v17/n8/full/7590953a.html (8293 words)

	Interaction of the Plasmid-Encoded Quinolone Resistance Protein Qnr with Escherichia coli DNA Gyrase -- Tran et al. 49 ...
		DNA but not unbound DNA was retained on filters (19, 31, 32).
		The antibiotic microcin B17 is a DNA gyrase poison: characterisation of the mode of inhibition.
		DNA gyrase-mediated wrapping of the DNA strand is required for the replication fork arrest by the DNA gyrase-quinolone-DNA ternary complex.
	aac.asm.org /cgi/content/full/49/1/118 (4584 words)

	biology - DNA polymerase III holoenzyme
		DNA polymerase III holoenzyme or Pol III is a holoenzyme that aids in DNA replication.
		2 β units which act as sliding DNA clamps.
		DNA Polymerase III can quickly conduct 5' to 3' replication.
	www.biologydaily.com /biology/DNA_holoenzyme (125 words)

	Creating a dynamic picture of the sliding clamp during T4 DNA polymerase holoenzyme assembly by using fluorescence ...
		The coordinated assembly of the DNA polymerase (gp43), the sliding clamp (gp45), and the clamp loader (gp44/62) to form the
		and gp44/62 and ATP and DNA with gp43.
		Many other proteins also used in DNA replication and recombination have been identified as toroid-shaped molecules (45).
	www.pnas.org /cgi/content/full/98/15/8368 (5040 words)

	The delta and delta ' Subunits of the DNA Polymerase III Holoenzyme Are Essential for Initiation Complex Formation and ...
		DNA was melted by heating at 90 °C for 10 min.
		Holoenzyme Reconstitution Activity Assay-- The replication activity of reconstituted holoenzyme was determined by measuring DNA synthesis from a primed M13
		DNA in the absence of SSB and filtered through a Bio-Gel A-5m column as described under "Experimental Procedures." Coomassie Blue-stained 4-20% gradient SDS-polyacrylamide gels of isolated ICs formed with holoenzyme reconstituted with
	www.jbc.org /cgi/content/full/276/37/35165 (5083 words)

	Amino-terminal sequences of sigma N (sigma 54) inhibit RNA polymerase isomerization -- Cannon et al. 13 (3): 357 -- ...
		The percentage of DNA shifted is indicated in the histogram.
		The interaction of holoenzymes with melted DNA in stable complexes was examined by use of S1 nuclease.
		in holoenzyme that is needed to unmask a holoenzyme single-strand
	www.genesdev.org /cgi/content/full/13/3/357 (5386 words)

	David Swigon's Research Interests
		The accumulation of such charges on one side of DNA is expected to lead to a partial neutralization of the negative charges of the DNA backbone and induce global bending of DNA.
		I have employed the method to study equilibrium configurations of a DNA o-ring [10], which is a miniplasmid that has a stress-free configuration in which the axial curve is a circle and hence mimics the highly curved structure of the kinetoplast DNA from a trypanosome.
		For extreme cases of twist reduction the agent causes untwisting of DNA that localizes at a site that is antipodal to the binding site of the agent, thereby producing an action at a distance of the type suggested by spectroscopic studies of the sequence-dependent structure of DNA block copolymers.
	rutchem.rutgers.edu /~swigon/research.html (1708 words)

	Ch432_Lec_6March
		This is particularly useful for removing RNA base-paired to DNA and for removing segments of DNA involving errors (such as thymine dimers formed by exposure to UV light).
		The actual replication of DNA occurs in the replisome, a complex including two Pol III enzymes, one to synthesize the leading strand and one to synthesize the lagging strand.
		This problem is solved by synthesising a sufficient length of DNA to allow the lagging single strand to loop around and come in parallel, instead of anti-parallel, to the leading strand.
	www.humboldt.edu /~rap1/C432.S02/C432Notes/C432n06Mar.html (948 words)

	Nucleotide-dependent interactions between a fork junction-RNA polymerase complex and an AAA+ transcriptional activator ...
		In (B), the position of the native DNA fork junction structure (at –11) and extra o-CuP reactivity at positions –14 and –13 are indicated by an arrow and unfilled arrowheads, respectively.
		Reactions were essentially as in Figure 3 but salmon sperm DNA was not included in the binding reactions.
		C of the clamp loader complex, involved in DNA replication (38,39).
	nar.oxfordjournals.org /cgi/content/full/32/15/4596 (5523 words)

	Transcription Initiation-Defective Forms of sigma 54 That Differ in Ability To Function with a Heteroduplex DNA ...
		Two different DNA probes were used for the gel mobility shift assays.
		The sequence that is cross-linked to promoter DNA upon UV irradiation (5) is indicated, as is a putative helix-turn-helix motif that appears to be involved in DNA binding (23).
		Cells were grown in a modified E medium that lacked sodium ammonium phosphate and was supplemented with acid-hydrolyzed Casamino Acids at 1 mg/liter as described previously (18).
	jb.asm.org /cgi/content/full/182/22/6503 (3346 words)

	Evidence against a Simple Tethering Model for Enhancement of Herpes Simplex Virus DNA Polymerase Processivity by ... (Site not responding. Last check: 2007-11-04)
		UL42-specific antiserum and by DNA polymerase assay (for Pol
		At time zero, excess unlabeled activated calf thymus DNA trap was added and the amount of labeled DNA-protein complexes remaining as a function of time was determined by nitrocellulose filter-binding activity as described in the legend to Fig.
		The effect of the UL42 protein on the DNA polymerase activity of the catalytic subunit of the DNA polymerase encoded by herpes simplex virus type 1.
	jvi.asm.org /cgi/content/full/76/20/10270 (7805 words)

	E. coli DNA Polymerase I (Site not responding. Last check: 2007-11-04)
		The E. coli DNA polymerase I is a DNA-dependent DNA polymerase that possesses both 3' -> 5' and 5' -> 3' exonuclease activities.
		Each of its three enzymatic activities are encapsulated into distinct domains of the holoenzyme, such that proteolytic deletions can be generated that lack one or more of the activities.
		DNA polymerase I was used frequently in the early days of recombinant DNA technology for radiolabeling DNA and synthesizing cDNA.
	arbl.cvmbs.colostate.edu /hbooks/genetics/biotech/enzymes/dnap1.html (162 words)

	Repression of GCN5 Histone Acetyltransferase Activity via Bromodomain-Mediated Binding and Phosphorylation by the ...
		The absence of Ku70/80 and/or DNA in the reaction abolished
		DNA template, transcription of nearby genes must be repressed.
		Assembly and DNA binding of recombinant Ku (p70/80) autoantigen defined by a novel monoclonal antibody specific for p70/80 heterodimers.
	mcb.asm.org /cgi/content/full/18/3/1349 (7516 words)

	Sergei Borukhov (Site not responding. Last check: 2007-11-04)
		In bacteria, the catalytically active multisubunit core enzyme (subunit composition: a2bb'w with the total molecular mass of ~400 kDa) joins s factor to form the holoenzyme (a2bb'ws), which is capable of specific promoter recognition and efficient initiation of transcription.
		The biochemical activities of this multifunctional enzyme include recognition and specific binding of promoter DNA, melting of the DNA double helix, binding of the nucleotide substrates and formation of the first phosphodiester bond, RNA chain extension and translocation along the DNA template, recognition of signals for pausing, RNA termination and antitermination.
		The latest major advance was the high-resolution X-ray structures of bacterial holoenzyme from extreme thermophiles, Thermus thermophilus (Tth) and Thermus aquaticus (Taq), and the structure of Taq holoenzyme-promoter DNA complex.
	www.downstate.edu /molecularbiology_genetics/s.borukhov.html (1052 words)

	Gyrase 2000G
		Incubation of 1 µg of catenated KDNA or supercoiled pUC19 DNA for 4 hrs.
		One unit of gyrase activity will supercoil 0.5 ug pBR-322 DNA in 30 min at 37 C. A 5X assay buffer and dilution buffer is included with the enzyme.
		The enzyme is shipped on dry ice and should be stored at -70 C. We also recommend that the enzyme be aliquoted after the first thaw (repeated rounds of freeze/thaw will lead to loss of activity); the enzyme activity is stable for 1-2 days (not longer) on ice.
	www.topogen.com /html/gyrase.html (275 words)

	Binding of transcriptional activators to sigma 54 in the presence of the transition state analog ADP-aluminum fluoride: ... (Site not responding. Last check: 2007-11-04)
		The arrow on the gel indicates the holoenzyme trapped activator complex in lane 6.
		O overnight at 37°C. Equal amounts of DNA were denatured and then electrophoresed through a 10% denaturing gel.
		DNA, even when initiating nucleotide was present (data not shown).
	www.genesdev.org /cgi/content/full/15/17/2282 (5721 words)

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