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Topic: DNA methyltransferase

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	DNA methyltransferase - Wikipedia, the free encyclopedia
		In biochemistry, the DNA methyltransferase (DNA MTase) family of enzymes catalyze the transfer of a methyl group to DNA.
		All the known DNA methyltransferases utilise S-adenosyl methionine (SAM) as the methyl donor.
		Four active DNA methyltransferases have been identified in mammals.
	en.wikipedia.org /wiki/DNA_methyltransferase (596 words)

	Mammalian DNA (Cytosine-5-)-methyltransferase Expressed in Escherichia coli, Purified and Characterized -- Tollefsbol ...
		DNA MTase has been shown in mice to be essential for embryonic survival (21) and has been proposed to play a role in general biological processes such as cellular aging(22), carcinogenesis(23), human genetic diseases(24), and evolution(17, 25).
		DNA was precipitated in 10% trichloroacetic acid, 5 mM sodium pyrophosphate for 15 min at 4 °C and washed 5 times on a Whatman GF/C filter with 5% trichloroacetic acid, 5 mM sodium pyrophosphate, and twice with 100% ethanol(29, 30).
		To demonstrate that the recombinant DNA MTase is indeed active with a preference for hemimethylated DNA, we reacted the partially purified enzyme with oligodeoxynucleotides in the presence of radioactive AdoMet, resolved the samples on agarose gels, and subjected the gels to autoradiography (Fig.
	www.jbc.org /cgi/content/full/270/31/18543 (6652 words)

	BioMed Central \| Full text \| Interactions within the mammalian DNA methyltransferase family
		Contrary to Dnmt3a or Dnmt3b, the isolated C-terminal region of Dnmt1 is catalytically inactive, despite the presence of the sequence motifs typical of active DNA methyltransferases.
		It is unable to methylate DNA in vitro and was shown not to be essential for de novo or maintenance methylation in ES cells even though it carries the key sequence motifs of DNA methyltransferases [11].
		Fatemi M, Hermann A, Pradhan S, Jeltsch A: The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA.
	www.biomedcentral.com /1471-2199/4/7 (4461 words)

	MethylGene :: DNA Methyltransferase (DNMT) (Site not responding. Last check: 2007-10-23)
		The punctuation of the human genome and the placing of methyl groups onto specific locations in DNA is achieved through a process called DNA methylation.
		DNA methylation tells cells which genes need to be expressed.
		The animation above demonstrates the regulation of tumour suppressor gene expression by DNA methyltransferase in the healthy and cancerous state, respectively, and how the disease state could be reversed by inhibiting DNMT.
	www.methylgene.com /content.asp?node=19 (450 words)

	Bioreagents.com: DNA Methyltransferase 1
		DNA methyltransferases (Dnmts) methylate the 5-position of cytosine in the context of CpG dinucleotides.
		DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation.
		Therefore, in addition to DNA methylation maintenance, Dnmt1 is thought to directly target transcriptionally repressive chromatin to the genome during S-phase DNA replication.
	bioreagents.com /index.cfm/fuseaction/products.detail/CatNbr/PA1-880 (266 words)

	A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast -- Yoder and Bestor 7 (2): 279 -- Human ...
		Trace levels of 5-methylcytosine persist in the DNA of mouse embryonic stem cells that are homozygous for null mutations in Dnmt1, the gene for the one previously recognized metazoan DNA methyltransferase.
		The DNA methyltransferases previously isolated from vertebrates (3-7), echinoderms (8) and flowering plants (9) comprise a C-terminal domain that is closely related to bacterial cytosine-5 restriction methyltransferases and a large N-terminal domain that has multiple regulatory functions (10).
		DNMT2 and Dnmt2 already bear the DNA methyltransferase consensus at the catalytic center and would be expected to be active, although activity may require cell type-specific post-transcriptional regulation or modification, the participation of accessory factors or specific alternative secondary structures in the DNA substrate.
	hmg.oxfordjournals.org /cgi/content/full/7/2/279 (4843 words)

	A Dnmt2-like protein mediates DNA methylation in Drosophila -- Kunert et al. 130 (21): 5083 -- Development
		DNA methylation in Drosophila is mediated by Dnmt2.
		The signal for DNA was not affected by the Su(var)3-9 mutation or by 5-azacytidine.
		of 5-methylcytosine in the genomic DNA from Dnmt2-overexpressing
	dev.biologists.org /cgi/content/full/130/21/5083 (4720 words)

	Section on Eukaryotic Gene Regulation (Site not responding. Last check: 2007-10-23)
		Differential gene expression correlated with differential DNA methylation, resulting in sharp transitions from methylated to unmethylated DNA at the open (active) promoter in both primary cells and tissues as well as in established cell lines.
		Moreover, treatment of cells with an inhibitor of DNA methyltransferase stimulated expression of the repressed gene.
		Therefore, DNA methylation was not the primary determinant of Sgy/Tead2 expression.
	nichddirsage.nichd.nih.gov:8080 /ar2004/pages/lmgr/segr.htm (2044 words)

	Expression of an Alternative Dnmt1 Isoform during Muscle Differentiation -- Aguirre-Arteta et al. 11 (10): 551 -- Cell ...
		Cardoso M. C., Leonhardt H. DNA methyltransferase is actively retained in the cytoplasm during early development.
		Szyf M., Rouleau J., Theberge J., Bozovic V. Induction of myogenic differentiation by an expression vector encoding the DNA methyltransferase cDNA sequence in the antisense orientation.
		Liu Y., Sun L., Jost J. In differentiating mouse myoblasts DNA methyltransferase is posttranscriptionally and posttranslationally regulated.
	cgd.aacrjournals.org /cgi/content/full/11/10/551 (5219 words)

	DNA Methylation -- Singal and Ginder 93 (12): 4059 -- Blood
		DNA methylation to oncogenesis appears to be mediated by one or
		DNA methylation clearly plays an important regulatory role in vertebrates, as evidenced by its vital role in embryonic development.
		Weiss A, Keshet I, Razin A, Cedar H: DNA demethylation in vitro: involvement of RNA.
	www.bloodjournal.org /cgi/content/full/93/12/4059 (6332 words)

	Bioreagents.com: DNA Methyltransferase 3b (Site not responding. Last check: 2007-10-23)
		DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides.
		Hypermethylation of DNA has also been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions.
		PA1-884 detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b.
	bioreagents.com /index.cfm/fuseaction/products.detail/CatNbr/PA1-884 (184 words)

	Arabidopsis MET1 Cytosine Methyltransferase Mutants -- Kankel et al. 163 (3): 1109 -- Genetics
		Genomic DNA samples prepared from wild-type MET1, ddm1-2, met1-1, and met1-2 homozygotes (Columbia strain) were digested with either MspI or HpaII and size fractionated by gel electrophoresis.
		After transfer to a nylon membrane, the DNA samples were hybridized sequentially with probes corresponding to the 180-bp centromere repeats (A), rRNA genes (B), or the MHC9.7/9.8 locus (C).
		After transfer to a nylon membrane, the DNA samples were hybridized sequentially with probes corresponding to the 180-bp centromere repeats (A) and the rRNA genes (B).
	www.genetics.org /cgi/content/full/163/3/1109 (6327 words)

	ipedia.com: Cytosine Article (Site not responding. Last check: 2007-10-23)
		Cytosine can be found as part of DNA, RNA or as a part of a nucleotide.
		In DNA and RNA, cytosine is paired with guanine.
		Cytosine can also be methylated into 5-methylcytosine by an enzyme called DNA methyltransferase.
	www.ipedia.com /cytosine.html (242 words)

	Abnormal Regulation of DNA Methyltransferase Expression during Colorectal Carcinogenesis -- De Marzo et al. 59 (16): ... (Site not responding. Last check: 2007-10-23)
		Kautiainen T. L., Jones P. DNA methyltransferase levels in tumorigenic and nontumorigenic cells in culture.
		Liu Y., Oakeley E. J., Sun L., Jost J. Multiple domains are involved in the targeting of the mouse DNA methyltransferase to the DNA replication foci.
		Rouleau J., MacLeod A. yf M. Regulation of the DNA methyltransferase by the Ras-AP-1 signaling pathway.
	cancerres.aacrjournals.org /cgi/content/full/59/16/3855 (3423 words)

	Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations
		At least two levels of regulation of the mouse DNA MeTase have been shown: at the transcriptional level, via its promoter, and at the post transcriptional level in a cell cycle dependent fashion.
		Using a probe generated by PCR of the human DNA MeTase cDNA, a human genomic library was screened and a clone of approximately 22 kilobases (kb) was isolated.
		First, the proposed catalytic domain of the human DNA MeTase is extremely homologous to all other cytosine DNA MeTases, even to those that are found in bacteria, and this catalytic domain is conserved within one complete exon in the human gene.
	www.osti.gov /energycitations/product.biblio.jsp?osti_id=133861 (370 words)

	Methylation Matters: Modeling a Manageable Genome -- Ordway and Curran 13 (4): 149 -- Cell Growth & Differentiation
		DNA in mouse cells (65), and it is concentrated at methylated
		Flynn J., Reich N. Murine DNA (cytosine-5-)-methyltransferase: steady-state and substrate trapping analyses of the kinetic mechanism.
		Kouzminova E., Selker E. dim-2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora.
	cgd.aacrjournals.org /cgi/content/full/13/4/149 (7956 words)

	CpG Island Hypermethylation in Human Colorectal Tumors Is Not Associated with DNA Methyltransferase Overexpression -- ...
		DNA methyltransferases correlate with the de novo methylation
		Teubner B., Schulz W. Regulation of DNA methyltransferase during differentiation of F9 mouse embryonal carcinoma cells.
		A. GROOPMAN, and A. DNA Methylation as a Cancer-Specific Biomarker: From Molecules to Populations
	cancerres.aacrjournals.org /cgi/content/full/59/10/2302 (4876 words)

	MGMT - O-6-methylguanine-DNA methyltransferase - Cancer GeneticsWeb
		Activity of O(6)-methylguanine-DNA methyltransferase in relation to p53 status and therapeutic response in ovarian cancer.
		O6-methylguanine-DNA methyltransferase in ovarian malignancy and its correlation with postoperative response to chemotherapy.
		Novel genetic polymorphisms in DNA repair genes: O(6)-methylguanine-DNA methyltransferase (MGMT) and N-methylpurine-DNA glycosylase (MPG) in lung cancer patients from Poland.
	www.cancerindex.org /geneweb/MGMT.htm (320 words)

	Role of the DNA Methyltransferase Variant DNMT3b3 in DNA Methylation -- Weisenberger et al. 2 (1): 62 -- Molecular ... (Site not responding. Last check: 2007-10-23)
		in vitro for unmethylated and hemimethylated DNA (10).
		DNA is mediated by the conserved cysteine-proline doublet in
		Soejima, K., Fang, W., and Rollins, B. DNA methyltransferase 3b contributes to oncogenic transformation induced by SV40T antigen and activated Ras.
	mcr.aacrjournals.org /cgi/content/full/2/1/62 (7278 words)

	Monoclonal Antibody to Dnmt (DNA methyltransferase) 3b
		Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development.
		Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality.
		Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation.
	www.imgenex.com /antibody_details.php?catalog=IMG-184 (658 words)

	Trial shows which brain cancer patients benefit from temozolomide
		There was a 46% survival rate at two years for the 46 patients in the group who had a methylated MGMT promoter (silent MGMT gene) but for the 60 patients with non-methylated promoter (active MGMT gene) status the two-year survival rate was only 13.8%, a statistically highly significant difference.
		So, it is bad news if the patient has non-methylated status because the DNA in these rogue cancer cells is being repaired as fast as the drug causes damage.
		This means the cancer cells are able to survive the drug's onslaught," said Dr. Hegi, who is also project leader at the national Centre of Competence in Research Molecular Oncology at ISREC in Epalinges, Switzerland.
	www.medicalnewstoday.com /medicalnews.php?newsid=14153 (844 words)

	A Dnmt2-like protein mediates DNA methylation in Drosophila -- Kunert et al. 130 (21): 5083 -- Development
		A Dnmt2-like protein mediates DNA methylation in Drosophila -- Kunert et al.
		DNA methyltransferase gene that has been termed Dnmt2.
		DNA Methyltransferase Gene dDnmt2 and Longevity of Drosophila
	dev.biologists.org /cgi/content/abstract/130/21/5083 (397 words)

	Monoclonal Antibody to Dnmt (DNA methyltransferase) 1
		Decrease of DNA methyltransferase 1 expression relative to cell proliferation in transitional cell carcinoma.
		Induction of DNA methylation and gene silencing by short interfering RNAs in human cells. Hiroaki Kawasaki, Kazunari Taira.
		Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development.
	www.imgenex.com /antibody_details.php?catalog=IMG-261 (964 words)

	5-Aza-Deoxycytidine Induces Selective Degradation of DNA Methyltransferase 1 by a Proteasomal Pathway That Requires the ...
		5-Aza-Deoxycytidine Induces Selective Degradation of DNA Methyltransferase 1 by a Proteasomal Pathway That Requires the KEN Box, Bromo-Adjacent Homology Domain, and Nuclear Localization Signal -- Ghoshal et al.
		DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), the maintenance
		methyltransferase, DNMT1, was rapidly degraded by the proteasomal
	mcb.asm.org /cgi/content/abstract/25/11/4727 (408 words)

	Physical and Functional Interactions between Daxx and DNA Methyltransferase 1-Associated Protein, DMAP1 -- Muromoto et ...
		DNA sequencing analysis revealed that one of the positive clones
		DNA methylation is known to contribute to transcriptional
		Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription.
	www.jimmunol.org /cgi/content/full/172/5/2985 (4930 words)

	Peptide Mapping of the Murine DNA Methyltransferase Reveals a Major Phosphorylation Site and the Start of Translation ...
		Peptide Mapping of the Murine DNA Methyltransferase Reveals a Major Phosphorylation Site and the Start of Translation
		The murine DNA methyltransferase catalyzes the transfer of methyl groups from S-adenosylmethionine to cytosines within d(CpG)
		Preferential Methylation of Unmethylated DNA by Mammalian de Novo DNA Methyltransferase Dnmt3a
	www.jbc.org /cgi/content/abstract/272/28/17851 (431 words)

	Role of the DNA Methyltransferase Variant DNMT3b3 in DNA Methylation -- Weisenberger et al. 2 (1): 62 -- Molecular ...
		Role of the DNA Methyltransferase Variant DNMT3b3 in DNA Methylation -- Weisenberger et al.
		Role of the DNA Methyltransferase Variant DNMT3b3 in DNA Methylation
		STAT3- and DNA methyltransferase 1-mediated epigenetic silencing of SHP-1 tyrosine phosphatase tumor suppressor gene in malignant T lymphocytes
	mcr.aacrjournals.org /cgi/content/abstract/2/1/62 (386 words)

	Recruitment of DNA methyltransferase I to DNA repair sites -- Mortusewicz et al., 10.1073/pnas.0501034102 -- ...
		Recruitment of DNA methyltransferase I to DNA repair sites -- Mortusewicz et al., 10.1073/pnas.0501034102 -- Proceedings of the National Academy of Sciences
		DNA lesions at defined subnuclear sites, we tested whether the
		major DNA methyltransferase (Dnmt1) or one of the two de novo
	www.pnas.org /cgi/content/abstract/0501034102v1 (253 words)

	Cytosine Molecule
		As cytosine triphosphate (CTP), it can act as a co-factor to enzymes, and can transfer a phosphate to convert
		In DNA and RNA, cytosine is paired with
		However, it is inherently unstable, and can change into uracil (
	www.worldofmolecules.com /life/cytosine.htm (92 words)

	News - Promoter Agent Indicates Which Patients Will Benefit from Temozolomide Therapy: Presented at AANS (Site not responding. Last check: 2007-10-23)
		Previous in vitro research showed that there is a synergistic effect between temozolomide and radiation, Dr. Hegi said.
		Tumor that carry a protein known as MGMT (O6-methylguanine-DNA methyltransferase) have also been previous shown to be resistant to temozolomide.
		Hegi and colleagues analyzed 206 tumor samples to determine their MGMT status.
	www.docguide.com /news/content.nsf/news/8525697700573E1885256FEB007FFC0D (702 words)

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