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Topic: DNA polymerase


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DNA
RNA

  
  Monserate Biotechnology Group - Products - Klenow Fragment (3´->5´ exo-), Taq DNA Polymerase, T4 DNA Ligase, ...
Monserate Biotechnology Group - Products - Klenow Fragment (3´->5´ exo-), Taq DNA Polymerase, T4 DNA Ligase, Bacteriophage F29 DNA Polymerase
Midas Value Pack (includes Klenow Fragment (3´->5´ exo-), Taq DNA Polymerase, T4 DNA Ligase, Φ29 DNA Polymerase)
If something you desire is not on the list please contact us atinfo@monseratebiotech.com
www.monseratebiotech.com /products.html   (66 words)

  
  Bathsheba Grossman - DNA Polymerase
The glass piece is a heavy 3 1/4" cube, with the DNA shown as a wireframe model and the enzyme as ribbons, with a translucent molecular surface outlining its form.
This particular polymerase specializes in damaged DNA: it doesn't give the best fidelity for a perfect strand, but it can work through some types of lesion that would stop a normal polymerase.
The DNA Polymerase Crystal comes with clear rubber feet to prevent scratches on your desk or mantel.
www.bathsheba.com /crystal/dnapolymerase   (0 words)

  
  Reference.com/Encyclopedia/DNA polymerase
DNA polymerase is considered to be a holoenzyme since it requires a magnesium ion as a co-factor to function properly.
DNA polymerases have highly-conserved structure, which means that their overall catalytic subunits vary, on a whole, very little from species to species.
η, ι, κ, and Rev1 are Y-family DNA polymerases and Pol ζ is a B-family DNA polymerase.
www.reference.com /browse/wiki/DNA_polymerase   (1181 words)

  
  DNA polymerase Summary
The DNA polymerase γ is encoded by the POLG (polymerase gamma) gene on chromosome 15 and replicates the DNA of the mitochondria.
DNA polymerases have highly-conserved structure, which means that their overall catalytic subunits vary, on a whole, very little from species to species.
DNA polymerase is considered to be a holoenzyme since it requires a Magnesium ion as a co-factor to function properly.
www.bookrags.com /DNA_polymerase   (2449 words)

  
  January 13: Genome Structure and DNA replication
DNA helicase - this enzyme unwinds the helical DNA at replication forks.
DNA polymerase III - this is the polymerase that does the bulk of the DNA replication using its 5'(3' polymerase activity.
DNA polymerase I - the function of this polymerase during replication is to remove the RNA primers (after they have done their job of initiating DNA replication) using a 5'--->3' exonuclease activity.
www.biosci.ohio-state.edu /~mgonzalez/Micro521/04.html   (1217 words)

  
  DNA polymerase - Wikipedia, the free encyclopedia
A DNA polymerase is an enzyme that assists in DNA replication.
DNA polymerase is considered to be a holoenzyme since it requires a Magnesium ion as a co-factor to function properly.
η, ι, κ, and Rev1 are Y-family DNA polymerases and Pol ζ is a B-family DNA polymerase.
en.wikipedia.org /wiki/DNA_polymerase   (1133 words)

  
 Polymerase - Wikipedia, the free encyclopedia
A polymerase (EC 2.7.7.6/7/19/48/49) is an enzyme whose central function is associated with polymers of nucleic acids such as RNA and DNA.
The most well-known function of a polymerase is the catalysis of production of new DNA or RNA from an existing DNA or RNA template, a process known as polymerization.
One particular polymerase, from the thermophilic bacterium, Thermus aquaticus (Taq, pronounced "tack") (PDB 1BGX, EC 2.7.7.7) is of vital commercial importance due to its use in the polymerase chain reaction, a widely-used technique of molecular biology.
en.wikipedia.org /wiki/Polymerase   (239 words)

  
 The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA ...
coli DNA polymerase I is involved primarily in the repair of
DNA whereas T7 DNA polymerase is responsible for the replication
DNA Polymerases that Propagate the Eukaryotic DNA Replication Fork
www.pnas.org /cgi/content/short/94/2/479   (730 words)

  
 Polymerase Chain Reaction - Xeroxing DNA
DNA polymerases, whether from humans, bacteria, or viruses, cannot copy a chain of DNA without a short sequence of nucleotides to "prime" the process, or get it started.
But the primers cannot bind to the DNA strands at such a high temperature, so the vial is cooled to 55 degrees C. At this temperature, the primers bind or "anneal" to the ends of the DNA strands.
Since the Taq polymerase works best at around 75 degrees C (the temperature of the hot springs where the bacterium was discovered), the temperature of the vial is raised.
www.accessexcellence.org /AB/IE/PCR_Xeroxing_DNA.html   (735 words)

  
 DNA Replication I
Like all known DNA polymerases, DNA polymerase I requires a primer from which to initiate replication and polymerizes deoxyribonucleotides into DNA in the 5' to 3' direction using the complementary strand as a template.
In 1969, John Cairns isolated a viable mutant (polA) lacking DNA polymerase I activity, an indication that polI is NOT the main enzyme used to replicate DNA.
Interestingly, E. coli temperature-sensitive (conditional lethal) mutants lacking the 5'-to-3' exonuclease activity of DNA polymerase I at non-permissive temperatures (40 degrees C) are NOT viable (remember, the polymerizing activity of DNA polymerase I is dispensible!).
oregonstate.edu /instruction/bb492/lectures/DNAI.html   (1362 words)

  
 DNA Replication
DNA polymerase I is the main polymerase involved in DNA repair, and plays a specialized role in DNA replication, using its 5' to 3' exonuclease activity.
The strand of DNA that is synthesized continuously is called the leading strand, and the strand that is synthesized discontinuously is called the lagging strand.
DNA polymerase I uses its 5' to 3' exonuclease activity to digest away the primer RNA, and replaces the primer with DNA by extending the strand from the adjacent Okazaki fragment.
www.emunix.emich.edu /~rwinning/genetics/replic3.htm   (724 words)

  
 Finnzymes - Phusion™ High-Fidelity DNA Polymerase   (Site not responding. Last check: )
Phusion DNA Polymerase exploits this dramatic increase in processivity, resulting in shorter extension times, more robust and high yield amplification, and the ability to do long templates in a fraction of time.
DNA polymerase was added to the primed template at a molar ratio of ~1:4,000 to initiate DNA synthesis at 72 °C. Samples taken at various times were diluted in gel loading dye, and analyzed on a MJ GeneWorks BaseStation ® (MJ Research).
Because of its robust performance, Phusion DNA Polymerase is capable of processing templates in the presence of additives such as DMSO, or in reactions containing impurities like debris from cell suspensions.
www.finnzymes.fi /products/pcr/phusion_high_fidelity_dna_polymerase.htm   (912 words)

  
 A half DNA ladder is a template for copying the whole.
DNA polymerase III, isolated by my son Tom, is the major enzyme that replicates DNA in E. coli.
DNA and proteins are the molecules of the cell nucleus.
A gene is a discrete sequence of DNA nucleotides.
www.dnaftb.org /dnaftb/text/20/index.html   (2929 words)

  
 DNA polymerase III holoenzyme Summary
The polymerase is able to operate in a continuous fashion on the leading parental strand that is unwinding.
DNA polymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication.
DNA Pol III is a component of the replisome, which is located at the replication fork.
www.bookrags.com /DNA_polymerase_III_holoenzyme   (756 words)

  
 DNA Synthesis
Histone H1 occupies the internucleosomal DNA and is identified as the linker histone.
DNA recombination refers to the phenomenon whereby two parental strands of DNA are spliced together resulting in an exchange of portions of their respective strands.
DNA damage can occur as the result of exposure to environmental stimuli such as alkylating chemicals or ultraviolet or radioactive irradiation and free radicals generated spontaneously in the oxidizing environment of the cell.
www.indstate.edu /thcme/mwking/dna.html   (4312 words)

  
 DNA Replication   (Site not responding. Last check: )
However, one of the newly formed DNAs must be synthesized in a 3' to 5' direction (since there are only two possible directions of synthesis and the two daughter strands are synthesized in opposite directions due to the antiparallel arrangement of the double helix).
Fragments of DNA result from this discontinuous method of DNA replication (i.e., synthesis occurs towards the existing daughter DNA and upon the inevitable collision with this strand the DNA polymerase is neither able to continue polymerizing nor stitch together the two adjacent DNA molecules).
Not only are DNA polymerases incapable of polymerizing DNA in the 3' to 5' direction, but they are also incapable of initiating DNA polymerization in the absence of an existing 3' -OH (this has something to do with the DNA polymerases being optimized toward high fidelity sequence duplication---mutation avoidance---rather than high versatility).
www.mansfield.ohio-state.edu /~sabedon/biol1060.htm   (1409 words)

  
 ChemCases: Cisplatin - 14. DNA
The three-dimensional structure of DNA was deduced in 1953 by James Watson and Francis Crick.
One of these is DNA polymerase I, which catalyzes the step-by-step addition of activated deoxyribonucleotide units to a DNA chain; the activated deoxyribonucleotide units for adenine, guanine, thymine, and cytosine are represented as dATP, dGTP, dTTP, and dCTP, respectively.
DNA is damaged in a variety of ways, and all cells have mechanisms by which to repair the damage.
www.chemcases.com /cisplat/cisplat14.htm   (1201 words)

  
 DNA Synthesis
Histone H1 occupies the internucleosomal DNA and is identified as the linker histone.
DNA recombination refers to the phenomenon whereby two parental strands of DNA are spliced together resulting in an exchange of portions of their respective strands.
DNA damage can occur as the result of exposure to environmental stimuli such as alkylating chemicals or ultraviolet or radioactive irradiation and free radicals generated spontaneously in the oxidizing environment of the cell.
web.indstate.edu /thcme/mwking/dna.html   (4312 words)

  
 Replication of DNA
DNA polymerase is described as being "template dependent" in that it will "read" the sequence of bases on the template strand and then "synthesize" the complementary strand.
DNA polymerase catalyzes the formation of the hydrogen bonds between each arriving nucleotide and the nucleotides on the template strand.
In addition to catalyzing the formation of Hydrogen bonds between complementary bases on the template and newly synthesized strands, DNA polymerase also catalyzes the reaction between the 5' phosphate on an incoming nucleotide and the free 3' OH on the growing polynucleotide (what we know is called a phosphodiester bond!).
www.ncc.gmu.edu /dna/replicat.htm   (909 words)

  
 SparkNotes: DNA Transcription: Prokaryotic Initiation
This process is different from that in DNA replication in which the parent helix remains separated until replication is done.
DNA transcription is also regulated, but it is triggered by different signals from those used to control DNA replication.
The sigma subunit of RNA polymerase is the part of the enzyme responsible for recognizing the signal on the DNA strand that tells the polymerase to begin synthesizing RNA.
www.sparknotes.com /biology/molecular/dnatranscription/section1.html   (1018 words)

  
 Bacterial DNA polymerase(s)
DNA polymerase I catalyzes the addition of a complementary dNTP to the 3'OH end of a polydeoxynucleotide chain.
However, as DNA polymerase I was characterized further, it became clear that the properties of this enzyme were not suitable for an enzyme with a central role in DNA replication:
The DNA polymerase III holoenzyme is the principal replicative enzyme in E.
www.mun.ca /biochem/courses/3107/Topics/DNA_polymerases.html   (2266 words)

  
 DNA Structure, Replication and Eukaryotic Chromatin Structure>
DNA replication is semi-conservative, one strand serves as the template for the second strand.
DNA single-stranded binding proteins - These proteins bind to the DNA as a tetramer and stabilize the single-stranded structure that is generated by the action of the helicases.
DNA Ligase - Nicks occur in the developing molecule because the RNA primer is removed and synthesis proceeds in a discontinuous manner on the lagging strand.
www.ndsu.nodak.edu /instruct/mcclean/plsc431/eukarychrom/eukaryo2.htm   (727 words)

  
 DNA Virus Replication
DNA replication is bidirectional (There are two replication forks per circular DNA genome and replication involves leading/lagging strands, Okazaki fragments, DNA ligase, etc.).
This process of DNA replication is very similar to that which occurs in the host cell - which is not surprising as the virus is using mainly host machinery except for the involvement of the T antigen.
However, although adenoviruses code for their own DNA polymerase, they use host factors in addition to viral proteins for DNA replication, and they use host RNA polymerase and RNA modification systems and so nucleic acid synthesis needs to be in the nucleus.
pathmicro.med.sc.edu /mhunt/dna1.htm   (3111 words)

  
 Techniques : Polymerase Chain Reaction
The location at which the primer bonds to the DNA determines the beginning of the section to be replicated.
The solution was to use Taq polymerase, derived from Thermus aquaticus, a bacterium that is native to the hot springs of Yellowstone.
Occasionally, molecules are improperly paired together, for example an A with a G or C. When this occurs, polymerase with the proofreading ability, attempts to remove incorrectly bonded molecules and fix such mistakes.
library.thinkquest.org /24355/data/details/techniques/polymerase.html   (379 words)

  
 BioMed Central | Full text | The DNA polymerase activity of Pol epsilon holoenzyme is required for rapid and efficient ...
Moreover, despite the decreased DNA synthesis, excess amounts of Pol α; are loaded onto the chromatin template in Pol ε-depleted extracts, indicative of the failure of proper assembly of DNA synthesis machinery at the fork [22].
Because this portion of the enzyme includes all known DNA polymerase and exonuclease motifs, these results suggest that the DNA polymerase activity of Pol ε; is dispensable for chromosomal DNA replication in yeast [15,16,27].
However, because the amino-terminal portion of Pol ε;, that is required for its DNA polymerase- and exonuclease activities, is dispensable for yeast DNA replication, repair, and viability [15,16,27], the role of Pol ε; during DNA replication has remained obscure.
www.biomedcentral.com /1471-2091/7/21   (5218 words)

  
 Recombinant Taq DNA Polymerase - ENZ-308
Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus.
DNA polymerase I, thermostable (EC (Taq polymerase 1).
Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of taq polymerase by the disaccharide trehalose.
www.prospec.co.il /~prospec/cart/catalog/rTaqDNA.html   (502 words)

  
 Engineered Enzyme Accelerates DNA Sequencing
Polymerases, which in their natural form help cells replicate genetic material, are used in sequencing to incorporate fluorescent tags into DNA to identify the locations of specific chemical units.
But Stanley Tabor and Charles Richardson of Harvard Medical School, with Office of Science support, applied fundamental discoveries of the structure and mechanisms of polymerases to create a new DNA polymerase, in which a single chemical change eliminated the bias against fluorescent bases—thereby producing more usable data than was generated previously.
Reference: Tabor, S., and Richardson, C. (1995) "A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides," Proc.
www.er.doe.gov /Sub/Accomplishments/Decades_Discovery/79.html   (414 words)

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