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DNA analysis |
 | | First, the basis for virtually all DNA sequencing was the dideoxy-chain terminating reaction, developed by Sanger and for which he received his second Nobel prize. |
 | | DNA polymerase and deoxynucleotides are added to the mixture, and the enzyme catalyzes the synthesis of the complementary DNA strand, starting at the primer and extending in the 3' direction. |
 | | When the sequencing reaction proceeds in the presence of a small amount of the dideoxynucleotide, the resulting chain-extension reaction products will consist of a set of molecules, all starting at the primer, all extending in the 3' direction, but terminating wherever the dideoxynucleotide happened to be incorporated, opposite its complementary nucleotide in the template. |
| faculty.washington.edu /dovichi/UBUBTpage/research/Methods/DNA_analysis.html (641 words) |
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