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DNase I Acutely Increases Cystic Fibrosis Sputum Elastase Activity and its Potential to Induce Lung Hemorrhage in Mice ... DNase I Acutely Increases Cystic Fibrosis Sputum Elastase Activity and its Potential to Induce Lung Hemorrhage in Mice The effect of different DNase I concentrations on sputum elastase activity is shown in Figure 2. In summary, the DNase I-mediated increase in CF sputum elastase activity is associated with an increase in the potential of ajrccm.atsjournals.org /cgi/content/full/157/2/464   (3348 words)

Ambion's Tips From the Bench: DNase I Demystified The smallest substrate for DNase I is a trinucleotide. Although DNase I is commonly perceived to cleave DNA nonspecifically, in practice it does show some sequence preference. While DNase I can be removed by phenol extraction, many researchers avoid this method for fear of loss of precious RNA sample during the extraction, and because it is time consuming and requires handling phenol, a hazardous chemical. www.ambion.com /techlib/tips/dnase1demystified.html   (1459 words)

DNase I proteins DNase I is an alpha,beta protein with two 6-stranded beta-pleated sheets packed against each other forming the core of a 'sandwich'-type structure. DNase I binds in the minor groove of the DNA duplex with an exposed loop region forming contacts in and along both sides of the minor groove and extending over a total of 6 base-pairs. As a consequence of DNase I binding, the minor groove opens by about 3 Angstroms and the duplex bends towards the major groove by about 20 degrees. www.biochem.ucl.ac.uk /bsm/prot_dna/family_descriptions/dnase1_family/dnase1_family.html   (156 words)

Recombinant DNase In 1990 Shak and colleagues cloned, sequenced and expressed a recombinant DNase 1 (rhDNase) and suggested that it may reduce the viscosity of CF sputum (Shak et al, 1990). When DNase is inhaled through a compressor and nebuliser, the viscosity of CF sputum is significantly reduced. Effect of aerosolized recombinant human DNase on exacerbations of respiratory symptoms and on pulmonary infection in patients with cystic fibrosis. www.cysticfibrosismedicine.com /htmldocs/CFText/dnase.htm   (0 words)

DNase I (RNase-Free) (M0303), Nucleases, NEB DNase I, (RNase-Free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´ -phosphorylated and 3´ -hydroxylated ends (1,2). Incubation of 100 units of DNase I with 10 ug of double-stranded RNA Ladder for 2 hours at 37°C resulted in the same electrophoretic profile as untreated RNA. Incubation of 2 units of DNase I with 10 ug of single-stranded RNA Ladder for 1 hour at 37°C resulted in the same electrophoretic profile as untreated RNA. www.neb.com /nebecomm/products/productM0303.asp   (232 words)

DNase Test - Gonorrhea - STD information from CDC When DNase is produced by organisms in the QuadFERM+ test, an acidic endproduct is formed and the pH indicator changes from red (alkaline) to yellow (acid) e.g., Figure 1. DNase tests may be performed on plate media which incorporate indicators. The DNase is used only as a supplemental test to aid in the identification of Neisseria and related species which have been characterized in other tests such as acid detection tests. www.cdc.gov /std/Gonorrhea/lab/tests/dnase.htm   (846 words)

[No title] The Dnase I which sedimented with the skeletons was catalytically inactive but could be reactivated by dissociation from the actin. Since the DNase I binding capacity of the skeletons corresponded to the number of actin protofilaments previously estimated by other methods, it seemed likely that the enzyme binding site was confined to one end of the filament. These results imply that the amino-terminal, DNase I-binding domain of the actin protomer is oriented toward the pointed end and is buried along the length of the actin filament. www.nursinglibrary.org /Portal/main.aspx?pageid=4024&sid=1457   (179 words)

Ambion, Inc. - Products Finally, DNase I is purified from bovine pancreas, one of the richest natural sources of RNase A. The threat of contaminating RNase activity in DNase I preparations requires that the enzyme be exhaustively purified. TURBO DNase was developed using a protein engineering approach that introduced amino acid changes into the DNA binding pocket of wild-type DNase I. These changes markedly increase the affinity of the protein for DNA. As a result, TURBO DNase is the enzyme of choice for eliminating plasmid DNA templates from transcription reactions, particularly for the synthesis of RNA standards used in RT-PCR and generating RNA for microinjection and transfection experiments. www.ambion.com /catalog/ProdGrp.html?fkApp=16&fkSubApp=49&fkProdGrp=288   (702 words)

DNase I Deoxyribonuclease I (RNase free) is a highly purified preparation of bovine pancreatic deoxyribonuclease I (DNase I). DNase I is activated by bivalent metal ions. In the presence of Mn2+, DNase I cleaves both strands of the DNA at approximately the same site yielding fragments of DNA that are blunt-ended or have protruding termini of only one or two nucleotides in length. www.rpbiotech.com /Mol_bio_reagents/DNase_I.htm   (265 words)

Hemoglobin Switching in Sheep: Only the gamma Gene is in the Active Conformation in Fetal Liver but all the {beta} and ... The molecular mechanism of differential expression of these genes in nuclei of fetal and adult erythroid cells has been investigated by analysis of their susceptibility to digestion by DNase I (genes that are in the conformation associated with active transcription are sensitive to this nuclease). The concentration of globin gene sequences in DNA from control and DNase I-digested nuclei was determined by annealing to synthetic DNAs and analogous cDNA probes derived from recombinant plasmids containing one of the sheep globin genes. Digestion by DNase I did not decrease the globin gene sequence concentration in residual DNA of spleen nuclei. www.pnas.org /cgi/content/abstract/75/12/5884   (399 words)

Higher Order Chromatin Structures in Maize and Arabidopsis -- Paul and Ferl 10 (8): 1349 -- THE PLANT CELL by exogenous DNase I in the subsequent lanes. DNA in lanes 2 to 6 was treated with 0.1, 0.2, 0.5, 1.0, and 2.0 µg/mL DNase I, respectively. DNA in lanes 2 to 5 was treated with 0.1, 0.5, 1.0, and 2.0 µg/mL DNase I, respectively. www.plantcell.org /cgi/content/full/10/8/1349   (6422 words)

RNase-Free DNase I | UBI.ca DNase I digestion is not required for RNA purified with HiBind® spin columns for applications such as Northern-Blot since this silica-based spin column technology efficiently removes the majority of the DNA. One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0.001 per minute per milliliter at 25°C, pH 5.0, with highly polymerized DNA as the substrate (1). DNase I is very sensitive and is subject to physical denaturation, so do not vortex this DNase I mixture. www.ubi.ca /cart/index.php/cPath/27_46_206   (0 words)

Cofilin and DNase I Affect the Conformation of the Small Domain of Actin -- Dedova et al. 82 (6): 3134 -- Biophysical ... DNase I binds to residues 38 to 52 in subdomain 2 DNase I binds to subdomains 2 and 4 (Kabsch et al., 1990 of proteolytic cleavage of the DNase I-binding loop (Strzelecka-Golaszewska www.biophysj.org /cgi/content/full/82/6/3134   (5699 words)

Identification of Amino Acids That Modulate Mannose Phosphorylation of Mouse DNase I, a Secretory Glycoprotein -- ... Analysis of the oligosaccharides of the immunoprecipitated DNase I and the calculation of the percent of the oligosaccharides Effect of single amino acid substitutions on oligosaccharide phosphorylation of murine DNase I. Constructs were prepared that replaced specific amino acids in murine DNase I with the equivalent residue present in bovine DNase I or with an alanine. The DNase I in the medium was immunoprecipitated, and one-half of the material was analyzed by SDS-PAGE. www.jbc.org /cgi/content/full/274/27/19309   (4281 words)

Human protein: P24855 - Deoxyribonuclease-1 precursor (EC ) (Deoxyribonuclease I) (DNase I) (Dornase alfa). EMBL ... Deoxyribonuclease-1 precursor (EC 3.1.21.1) (Deoxyribonuclease I) (DNase I) (Dornase alfa). Structure of the human deoxyribonuclease I (DNase I) gene: identification of the nucleotide substitution that generates its classical genetic polymorphism. DNase I mediates internucleosomal DNA degradation in human cells undergoing drug-induced apoptosis. harvester.embl.de /harvester/P248/P24855.htm   (1210 words)

DNase-Free Certification - Mo Bio Labs - Services A nuclease is an enzyme that degrades nucleic acids; DNase is a nuclease that degrades DNA. A DNA standard is exposed to DNase is used to represent the positive control. The positive control should be very smeared, indicating the effect of DNase contamination on the DNA standard pool that is used for all the reactions. www.mobio.com /services/services_view.php?id=3   (0 words)

Protein-DNA interactions DNase I has a small degree of specificity, so that it does not cut uniformly at all sites. Other agents that break or damage DNA can be used in addition to DNase I. Dimethyl sulfate (DMS) methylates G residues, creating an adduct that can be broken chemically. DNase I can only be used under conditions that support its activity (presence of Mg and Ca ions). www.biochem.arizona.edu /classes/bioc568/protein_dna_interactions.htm   (1973 words)

Antitumor agent and dnase invention [0007] However, that a DNase per se is used as an antitumor agent, and that a DNase does not act on normal cells but specifically acts on cancer cells, is unknown. Furthermore, although a restriction enzyme is known to digest a DNA at a specific recognition site, that a restriction enzyme per se is used as an antitumor agent, and that a restriction enzyme does not act on normal cells but specifically acts on cancer cells, is unknown. [0012] According to a preferred embodiment of the present invention, the DNase is used in the form of a complex of the DNase and a liposome. www.freshpatents.com /Antitumor-agent-and-dnase-dt20061012ptan20060228347.php   (834 words)

AMPD1 DNase I Amplification Grade Because PCR can detect even a single molecule of DNA, RNA samples should be digested with DNase I before RT-PCR, and parallel reactions should be run without reverse transcriptase to check for amplification of contaminating DNA. DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Many commercial DNase I formulations are contaminated with residual RNases. www.sigmaaldrich.com /catalog/search/ProductDetail/SIGMA/AMPD1   (223 words)

Ambion TechNotes 9(5): An Animal-Free DNase I Recombinant DNase I (rDNase I) Digestion of DNA to undetectable levels is important in many molecular biology applications. The two DNase I treatments gave essentially identical results and were therefore averaged and compared to the untreated sample. The results revealed that "...with the exception of Ambion's RNase-free DNase I, the integrity of cRNA from in vitro transcription reactions was compromised and was still contaminated with DNA. www.ambion.com /techlib/tn/95/9515.html   (779 words)

Deoxyribonuclease I - Worthington Biochemical Product Catalog The average chain length of a limit digest is a tetranucleotide. DNase is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. Recommended storage temperature for all grades of Worthington DNases is 2-8°C. Product code DPRFS may be stored at -20°C. For long term storage in solution, Product Codes D and DPFF may be dissolved in 5mM acetate, 1mM calcium, pH 4.5 and stored in single use aliquots at -20°C or -70°C for up to one year. www.worthington-biochem.com /DNASE/cat.html   (556 words)

RNase-Free DNase I, EPICENTRE® Biotechnologies RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation. Unit Definition: Using the standard 50 µl assay volume, one Molecular Biology Unit (MBU) of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutes at 37°C in 33 mM Tris-acetate (pH 7.8), 66 mM potassium acetate, 10 mM magnesium acetate, 0.5 mM DTT, and 2 mM CaCl DNA removal from in vitro transcription reactions using RNase-Free DNase I. A linearized DNA template was transcribed using T7 RNA polymerase according to standard in vitro transcription conditions. www.epibio.com /item.asp?ID=348&CatID=2&SubCatID=92   (297 words)

DNase I-Senstive Ultrastructural Probes of Active DNA Sites within Human Intact Cells. DNase I-Senstive Ultrastructural Probes of Active DNA Sites within Human Intact Cells. DNA sites active for gene transcription are sensitive to DNase I digestion. Frenster JH, "Single-Cell Analysis of DNase I-Sensitive Sites during Neoplastic and Normal Cell Differentiation within Human Bone Marrow". www.euchromatin.org /DNase01.htm   (718 words)

A distant evolutionary relationship between bacterial sphingomyelinase and mammalian DNase I -- MATSUO et al. 5 (12): ... A distant evolutionary relationship between bacterial sphingomyelinase and mammalian DNase I -- MATSUO et al. A distant evolutionary relationship between bacterial sphingomyelinase and mammalian DNase I specific to mammalian DNase I and bacterial SMase. www.proteinscience.org /cgi/content/abstract/5/12/2459   (650 words)

MSD - entry 2dnj XRAY Structure of DEOXYRIBONUCLEASE I (DNASE I) (E.C.3.1.21.1) COMPLEXED WITH DNA (5'-D(GP CP GP AP TP CP GP CP)-3') at 2 A resolution. 2 A structure of a DNase I-octamer complex. DEOXYRIBONUCLEASE I (DNASE I) (E.C.3.1.21.1) COMPLEXED WITH DNA (5'-D(GP CP GP AP TP CP GP CP)-3') www.ebi.ac.uk /msd-srv/msdlite/atlas/summary/2dnj.html   (97 words)

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