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Topic: Deoxyribonuclease

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	Worthington Deoxyribonuclease II
		Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa.
		The enzyme is a glycoprotein endonuclease with dimeric structure.
		Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966).
	www.worthington-biochem.com /HDAC/cat.html (121 words)

	Deoxyribonuclease - Wikipedia, the free encyclopedia
		A deoxyribonuclease (DNase, for short) is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone.
		A wide variety of deoxyribonucleases are known, which differ in their substrate specificities, chemical mechanisms, and biological functions.
		Deoxyribonuclease I cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides.
	en.wikipedia.org /wiki/Deoxyribonuclease (222 words)

	DFFB - DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
		Expression of caspase-activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the cochlea of cisplatin (CDDP)-treated guinea pigs.
		The induction of the deoxyribonuclease activity was correlated with caspase-3 activation and caspase-3- mediated degradation of inhibitor of caspase-activated deoxyribonuclease.
		Identification of caspase-3 and caspase-activated deoxyribonuclease in rat blastocysts and their implication in the induction of chromatin degradation (but not nuclear fragmentation) by high glucose.
	www.ihop-net.org /UniPub/iHOP/gg/87686.html (4746 words)

	Patent 6,664,379
		Therefore, it is entirely within the scope of the invention to use the inhibitors disclosed herein to treat a composition which may have nucleases in it, or even a composition which a research is confident does not have nucleases in it, but which the researcher wishes to treat out of an abundance of caution.
		In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease, as described elsewhere in the specification.
		Deoxyribonucleases that can be inhibited and/or inactivated using the present invention include, but are not limited to, DNase 1, S1 nuclease, and micrococcal nuclease.
	www.pharmcast.com /Patents100/Yr2003/Dec2003/121603/6664379_Cocktail121603.htm (4781 words)

	Recombinant human deoxyribonuclease for cystic fibrosis
		Recombinant human deoxyribonuclease (rhDNase) was developed to thin out the sputum (phlegm) produced by infections that can block air passages, causing further infection.
		Recombinant human deoxyribonuclease (rhDNase) is currently used to treat pulmonary disease (the major cause of morbidity and mortality) in cystic fibrosis.
		To determine whether the use of rhDNase in cystic fibrosis is associated with improved mortality and morbidity compared to placebo or other mucolytics and to identify any adverse events associated with its use.
	www.cochrane.org /reviews/en/ab001127.html (552 words)

	Inhalt - Frameversion
		Recent data indicate that deoxyribonuclease I (DNase I) is identical to the apoptotic endonuclease responsible for the internucleosomal DNA degradation.
		The expression and distribution of deoxyribonuclease I (DNase I) in rat parotid gland, kidney, small intestine and keratinized epithelium was further analysed at the level of its mRNA by in situ hybridization and correlated to immunohistochemical results using polyclonal anti-DNase I antibodies.
		The expression of deoxyribonuclease I (DNase I) in various rat tissues was screened by use of a cDNA-probe of rat parotid DNase I and monospecific polyclonal antibodies.
	www.ruhr-uni-bochum.de /anat1/pub.htm (13140 words)

	Worthington Deoxyribonuclease I
		Deoxyribonuclease from beef pancreas, DNase I, first crystallized by Kunitz (1950), is an endonuclease, splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'.
		Bollum (1965) reports degradation of synthetic homopolymer complexes by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor (Lindberg and Skoog 1970), which according to Lazarides and Lindberg (1974) is actin.
		Composition: There are four deoxyribonucleases of beef pancreas: A, B, C, and D (Salnikow, Moore, and Stein 1970; Salnikow and Murphy 1973; Liao 1974).
	www.worthington-biochem.com /DNASE (3895 words)

	Detection of Deoxyribonuclease I Along the Secretory Pathway in Paneth Cells of Human Small Intestine -- Shimada et al. ... (Site not responding. Last check: 2007-09-10)
		Nadano D, Yasuda T, Kishi K (1993) Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method.
		Shimada O, Suzuki S, Tosaka–Shimada H, Ishikawa H (in press) Detection of deoxyribonuclease I in a hormone-secretory pathway of pituitary cells in humans and rats.
		Yasuda T, Kishi K, Yanagawa Y, Yoshida A (1995) Structure of the human deoxyribonuclease I (DNase I) gene: identification of the nucleotide substitution that generates its classical genetic polymorphism.
	www.jhc.org /cgi/content/full/46/7/833 (3540 words)

	Techniques for preparing specimens for bacterial assays - US Patent 5364763
		The method of claim 8 wherein said deoxyribonuclease is deoxyribonuclease I. The method of claim 9 wherein said deoxyribonuclease I is purified from bovine pancreas.
		The method of claim 24 wherein said deoxyribonuclease is deoxyribonuclease I. The method of claim 25 wherein said deoxyribonuclease I is purified from bovine pancreas.
		A kit for assaying a mucoid secretion specimen for the presence of a selected bacterial species, including in a first container a composition comprising a disulfide bond reducing agent and in a second container a composition comprising a DNA digestion agent for liquifying said specimen; and means for determining the presence of said bacterial species.
	www.patentstorm.us /patents/5364763.html (6777 words)

	Human protein: P24855 - Deoxyribonuclease-1 precursor (EC ) (Deoxyribonuclease I) (DNase I) (Dornase alfa). EMBL ...
		Deoxyribonuclease-1 precursor (EC 3.1.21.1) (Deoxyribonuclease I) (DNase I) (Dornase alfa).
		Structure of the human deoxyribonuclease I (DNase I) gene: identification of the nucleotide substitution that generates its classical genetic polymorphism.
		The molecular basis for genetic polymorphism of human deoxyribonuclease I: identification of the nucleotide substitution that generates the fourth allele.
	harvester.embl.de /harvester/P248/P24855.htm (1269 words)

	Journal of Cerebral Blood Flow & Metabolism - Abstract of article: Induction of Caspase-Activated Deoxyribonuclease ...
		Deoxyribonucleic acid fragmentation at nucleosomal junctions is a hallmark of neuronal apoptosis in ischemic brain injury, for which the mechanism is not fully understood.
		This in vitro deoxyribonuclease activity was completely inhibited by purified inhibitor of caspase-activated deoxyribonuclease protein, the specific endogenous inhibitor of caspase-activated deoxyribonuclease, or by caspase-activated deoxyribonuclease immunodepletion.
		The induction of the deoxyribonuclease activity was correlated with caspase-3 activation and caspase-3−mediated degradation of inhibitor of caspase-activated deoxyribonuclease.
	www.nature.com /jcbfm/journal/v22/n1/abs/9591187a.html (256 words)

	[No title] (Site not responding. Last check: 2007-09-10)
		This alternate endonuclease was identified as deoxyribonuclease II (DNase II; Barry, M. and Eastman, A. Archives of
		Thus, it remains to be determined which specific endonuclease is involved in apoptosis, The enzyme referred to herein as deoxyribonuclease IIa (DNA IIa) was isolated and purified and the amino acid sequence determined (PCT/US97/18262).
		DETAILED DESCRIPTION OF THE INVENTION The existence of a deoxyribonuclease II (DNase II) enzyme as a protein of lysosomal origin that is involved in cellular digestion of foreign DNA has been known for many years.
	www.wipo.int /cgi-pct/guest/getbykey5?KEY=01/75082.011011&ELEMENT_SET=DECL (2431 words)

	CHEST: Efficacy of Recombinant Human Deoxyribonuclease I in the Hospital Management of Respiratory Syncytial Virus ... (Site not responding. Last check: 2007-09-10)
		Objective: To evaluate the effect of recombinant human deoxyribonuclease I (rhDNase) in shortening the length of the hospitalization and improving the chest radiographs (CXRs) in hospitalized infants with respiratory syncytial virus (RSV) infection as a result of its mucolytic properties.
		Some airways become partially or completely occluded, leading to air trapping and hyperinflation or atelectasis.[10] Due to lysis of inflammatory cells, DNA is present in large amounts (3 to 14 mg/mL) in the mucous plugs.
		We hypothesized that therapy with rhDNase may result in shorter length of hospitalization, improved clinical scores, and improved chest radiographs (CXRs) in hospitalized infants with RSV infection as a result of its mucolytic properties.
	www.findarticles.com /p/articles/mi_m0984/is_1_120/ai_76994066 (1253 words)

	[No title]
		Deoxyribonuclease I (DNase I) (EC:3.1.21.1) [1] is a vertebrate enzyme which catalyzes the endonucleolytic cleavage of double-stranded DNA to 5'- phosphodinucleotide and 5'-phosphooligonucleotide end-products.
		O55070 Deoxyribonuclease gamma precursor (EC 3.1.21.-) (DNase gamma) (Deoxyribonuclease I-like 3) (DNase I homolog protein DHP2) (Liver and spleen DNase) (LS-DNase) (LSD)
		P24855 Deoxyribonuclease-1 precursor (EC 3.1.21.1) (Deoxyribonuclease I) (DNase I) (Dornase alfa)
	www.ebi.ac.uk /interpro/IEntry?ac=IPR008185 (554 words)

	Deoxyribonuclease II is required during the phagocytic phase of apoptosis and its loss causes perinatal lethality
		Deoxyribonuclease II is required during the phagocytic phase of apoptosis and its loss causes perinatal lethality
		Deoxyribonuclease II (DNase II) is one of many endonucleases implicated in DNA digestion during apoptosis.
		These results demonstrate that DNase II is not required for the generation of the characterisitic DNA fragmentation that occurs during apoptosis but is required for degrading DNA of dying cells and this function is necessary for proper fetal development.
	www.nature.com /cdd/journal/v9/n9/abs/4401056a.html (339 words)

	Sp1 and Sp3 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 ... (Site not responding. Last check: 2007-09-10)
		Yasuda, T., Nadano, D., Sawazaki, K. and Kishi, K. (1992) Genetic polymorphism of human deoxyribonuclease II (DNase II): low activity levels in urine and leukocytes are due to an autosomal recessive allele.
		Barry, M.A. and Eastman, A. (1993) Identification of deoxyribonuclease II as an endonuclease involved in apoptosis.
		Krieser, R.J., MacLea, K.S., Longnecker, D.S., Fields, J.L., Fiering, S. and Eastman, A. (2002) Deoxyribonuclease II alpha is required during the phagocytic phase of apoptosis and its loss causes perinatal lethality.
	content.febsjournal.org /cgi/content/full/270/8/1855 (4467 words)

	Chest: Effects of Streptokinase and Deoxyribonuclease on Viscosity of Human Surgical and Empyema Pus().@ HighBeam ... (Site not responding. Last check: 2007-09-10)*
		Effects of Streptokinase and Deoxyribonuclease on Viscosity of Human Surgical and Empyema Pus(*).
		Study objective: To investigate the effects of streptokinase and deoxyribonuclease (DNase) on the viscosity of pus to assess whether the DNase in the old preparation of streptokinase-streptodornase used intrapleurally to treat empyema was contributing to easier drainage of pus compared with purified streptokinase.
		Patients: Pus from three patients with surgically drained soft tissue abscesses and from six patients with empyema thoracis of varying etiology was studied.
	www.highbeam.com /library/doc0.asp?DOCID=1G1:63255012&refid=holomed_1 (199 words)

	Nucleases: Deoxyribonuclease I (RNase free)
		Deoxyribonuclease I (DNase I) is an endonuclease that digests single- and double-stranded DNA.
		It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5-phosphate and 3'-OH groups.
		Studies of DNA-protein interactions by Deoxyribonuclease I footprinting (1).
	www.fermentas.com /catalog/modifyingenzymes/dribonui.htm (371 words)

	Effects of deoxyribonuclease I on murine melano...
		Introduction: Importance of the nucleases in the regulation of cellular growth (through the apoptosis) makes necessary to investigate their utility to control tumoral growth.
		Objective: We evaluated the effect that deoxyribonuclease (DNase) had on cells of the melanoma murine B16
		Material and methods: the cells were incubated at different times and concentrations of the DNase and then their viability, growth and damage to the DNA were evaluated.
	www.imbiomed.com.mx /INER/Inv14n2/english/Zin012-01.html (229 words)

	Effects of Streptokinase and Deoxyribonuclease on Viscosity of Human Surgical and Empyema Pus -- Simpson et al. 117 ... (Site not responding. Last check: 2007-09-10)
		Effects of Streptokinase and Deoxyribonuclease on Viscosity of Human Surgical and Empyema Pus -- Simpson et al.
		and deoxyribonuclease (DNase) on the viscosity of pus to assess
		Tillett, WS, Sherry, S (1949) The effect in patients of streptococcal fibrinolysin (streptokinase) and streptococcal deoxyribonuclease on fibrinous, purulent and sanguineous pleural exudations.
	www.chestjournal.org /cgi/content/full/117/6/1728 (2408 words)

	Influence of disulfide-reducing agents on fractionation of the chromatin complex by endogenous nucleases and ... (Site not responding. Last check: 2007-09-10)
		Influence of disulfide-reducing agents on fractionation of the chromatin complex by endogenous nucleases and deoxyribonuclease I in aging mice.
		The amount of DNA that could be released from the pellet fraction by disulfide-reducing agents was significantly greater with old than with young adult mice.
		The combined results suggest the existence of a supranucleosomal alteration in chromatin structure during aging.
	www.arclab.org /medlineupdates/abstract_7130639.html (142 words)

	Deoxyribonuclease I (DNase I) Typing from Semen Stains: Low Enzyme Activity in Vaginal Fluids Does Not Interfere with ...
		Deoxyribonuclease I (DNase I) Typing from Semen Stains: Low Enzyme Activity in Vaginal Fluids Does Not Interfere with Seminal DNase I Typing from Mixture Stains
		We describe the use of deoxyribonuclease I (DNase I) polymorphism for individualization of semen in body fluid stain mixtures, as a means of providing new and more useful information to practicing forensic biologists as a genetic marker.
		An evaluation of DNase I typing by IEF-PAGE and DAFO was also performed on casework samples submitted to our laboratory, and the results showed that DNase I was expected to be one of the most useful individualization marker of semen in practical application.
	www.astm.org /JOURNALS/FORENSIC/PAGES/2000.htm (380 words)

	Deoxyribonuclease I-like 1, Homo sapiens - UniGene Hs.401929
		Deoxyribonuclease I-like 1, Homo sapiens - UniGene Hs.401929
		Tissues and development stages from this gene's sequences survey gene expression.
		Homo sapiens deoxyribonuclease I-like 1 (DNASE1L1), transcript variant 1, mRNA
	www.ncbi.nlm.nih.gov /UniGene/clust.cgi?ORG=Hs&CID=401929 (267 words)

	Serum deoxyribonuclease I activity can be used as a sensitive marker for detection of transient myocardial ischaemia ... (Site not responding. Last check: 2007-09-10)
		Serum deoxyribonuclease I activity can be used as a sensitive marker for detection of transient myocardial ischaemia induced by percutaneous coronary intervention -- Arakawa et al., 10.1093/eurheartj/ehi228 -- European Heart Journal
		Serum deoxyribonuclease I activity can be used as a sensitive marker for detection of transient myocardial ischaemia induced by percutaneous coronary intervention
		deoxyribonuclease I (DNase I) activity in the early stage of
	eurheartj.oxfordjournals.org /cgi/content/abstract/ehi228v1 (379 words)

	Bioline International Official Site (site up-dated regularly) (Site not responding. Last check: 2007-09-10)
		The substrate was obtained by radioactive PCR and it was found to be more sensitive in the detection of deoxyribonuclease activities (more than 10-5 units of a commercial DNAse I in 20 min of reaction) than the non-radioactive substrates commonly used for these purposes.
		Current analytical quality control methods for the detection of undesirable deoxyribonuclease activities in final preparations of biological reagents such as restriction and modifying enzymes, acetylated BSA, etc, involve the use of different DNA substrates [1-3].
		Enough substrate to test 50-70 samples for the presence of contaminant deoxyribonuclease activities could be obtained with only one PCR reaction.
	www.bioline.org.br /request?ba99007 (1152 words)

	Androgen Ablation Leads to an Upregulation and Intranuclear Accumulation of Deoxyribonuclease I in Rat Prostate ... (Site not responding. Last check: 2007-09-10)
		Identification of deoxyribonuclease II as an endonuclease involved in apoptosis.
		Human serum deoxyribonuclease I (DNase I) polymorphism: pattern similarities among isoenzymes from serum, urine, kidney, liver, and pancreas.
		Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method.
	www.jcb.org /cgi/content/full/137/4/909 (7635 words)

	BD - Diagnostic Systems: DNase Test Agar with Toluidine Blue
		DNase Test Agar with Toluidine Blue is used to aid in the differentiation of Serratia species, isolated from clinical sources, which may be incorrectly identified as Enterobacter and Klebsiella species.
		The DNase Test Agar medium was recommended for the detection of extracellular deoxyribonuclease activity of fungi and bacteria, especially staphylococci, enteric bacilli, and pseudomonads.
		Examine plates after 18 to 24 h for amount of growth and presence and intensity of zones of deoxyribonuclease activity.
	www.bd.com /ds/productCenter/221856.asp (556 words)

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